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Protein

Epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthase

Gene

SCO5223

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of the sesquiterpenoid antibiotic albaflavenone. Catalyzes the two-step allylic oxidation of epi-isozizaene to albaflavenone. First carries out a non-stereo-specific oxidation of epi-isozizaene to give a mixture of the albaflavenol epimers ((5R)-albaflavenol and (5S)-albaflavenol), each of which can serve as substrate for the second oxidation to yield albaflavenone. This is quite different from most other P450s which catalyze regio- and stereospecific oxidation. Displays also a farnesene synthase activity with farnesyl diphosphate (FPP) as substrate.2 Publications

Catalytic activityi

+-epi-isozizaene + 2 NADPH + 2 O2 = albaflavenone + 2 NADP+ + 3 H2O.1 Publication
(2E,6E)-farnesyl diphosphate = (E)-beta-farnesene + diphosphate.1 Publication

Cofactori

Mg2+1 Publication, Ca2+1 Publication, Mn2+1 PublicationNote: Divalent metal cations. Mg2+, Ca2+ or Mn2+ ions for farnesene synthase activity.1 Publication

Kineticsi

Kcat is 0.019 s(-1) with farnesyl diphosphate (FPP) as substrate (in the presence of magnesium ions at 30 degrees Celsius and at pH 5.5).

  1. KM=16.8 µM for FPP (in the presence of magnesium ions at 30 degrees Celsius and at pH 5.5)1 Publication

    pH dependencei

    Optimum pH is between 7 and 8.2 for monooxygenase activity and between 5.5 and 6.5 for farnesene synthase activity.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi410Iron (heme axial ligand)1

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Magnesium, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13905.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthase (EC:1.14.13.106, EC:4.2.3.47)
    Alternative name(s):
    Cytochrome P450 170A1
    Short name:
    CYP170A1
    Gene namesi
    Ordered Locus Names:SCO5223
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    Proteomesi
    • UP000001973 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Pathology & Biotechi

    Disruption phenotypei

    Disruption abolishes biosynthesis of both albaflavenone and the albaflavenols, but not epi-isozizaene.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi253D → A: Loss of any farnesene synthase activity, whereas the P450 monooxygenase activity is retained. 1 Publication1
    Mutagenesisi254D → A: Loss of any farnesene synthase activity, whereas the P450 monooxygenase activity is retained. 1 Publication1
    Mutagenesisi257D → A: Loss of any farnesene synthase activity, whereas the P450 monooxygenase activity is retained. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004186201 – 461Epi-isozizaene 5-monooxygenase/(E)-beta-farnesene synthaseAdd BLAST461

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi100226.SCO5223.

    Structurei

    Secondary structure

    1461
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi35 – 42Combined sources8
    Helixi44 – 51Combined sources8
    Helixi52 – 54Combined sources3
    Beta strandi56 – 62Combined sources7
    Beta strandi65 – 70Combined sources6
    Helixi73 – 81Combined sources9
    Beta strandi85 – 87Combined sources3
    Turni89 – 92Combined sources4
    Helixi93 – 96Combined sources4
    Turni97 – 101Combined sources5
    Beta strandi102 – 104Combined sources3
    Helixi117 – 119Combined sources3
    Helixi120 – 123Combined sources4
    Turni125 – 127Combined sources3
    Turni129 – 131Combined sources3
    Helixi132 – 145Combined sources14
    Helixi154 – 170Combined sources17
    Helixi177 – 192Combined sources16
    Helixi220 – 234Combined sources15
    Helixi244 – 248Combined sources5
    Helixi261 – 292Combined sources32
    Helixi294 – 308Combined sources15
    Helixi315 – 318Combined sources4
    Helixi322 – 334Combined sources13
    Beta strandi341 – 344Combined sources4
    Beta strandi349 – 351Combined sources3
    Beta strandi354 – 356Combined sources3
    Beta strandi361 – 364Combined sources4
    Helixi366 – 370Combined sources5
    Turni373 – 375Combined sources3
    Turni377 – 380Combined sources4
    Helixi384 – 387Combined sources4
    Helixi389 – 392Combined sources4
    Beta strandi405 – 409Combined sources5
    Helixi413 – 430Combined sources18
    Beta strandi431 – 435Combined sources5
    Beta strandi440 – 453Combined sources14
    Beta strandi455 – 460Combined sources6

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3DBGX-ray2.60A/B1-461[»]
    3EL3X-ray3.30A/B1-461[»]
    ProteinModelPortaliQ9K498.
    SMRiQ9K498.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9K498.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiENOG41068W2. Bacteria.
    COG2124. LUCA.
    HOGENOMiHOG000043114.
    InParanoidiQ9K498.
    KOiK12645.
    OMAiEMSEWAS.
    OrthoDBiPOG091H09CE.
    PhylomeDBiQ9K498.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9K498-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTVESVNPET RAPAAPGAPE LREPPVAGGG VPLLGHGWRL ARDPLAFMSQ
    60 70 80 90 100
    LRDHGDVVRI KLGPKTVYAV TNPELTGALA LNPDYHIAGP LWESLEGLLG
    110 120 130 140 150
    KEGVATANGP LHRRQRRTIQ PAFRLDAIPA YGPIMEEEAH ALTERWQPGK
    160 170 180 190 200
    TVDATSESFR VAVRVAARCL LRGQYMDERA ERLCVALATV FRGMYRRMVV
    210 220 230 240 250
    PLGPLYRLPL PANRRFNDAL ADLHLLVDEI IAERRASGQK PDDLLTALLE
    260 270 280 290 300
    AKDDNGDPIG EQEIHDQVVA ILTPGSETIA STIMWLLQAL ADHPEHADRI
    310 320 330 340 350
    RDEVEAVTGG RPVAFEDVRK LRHTGNVIVE AMRLRPAVWV LTRRAVAESE
    360 370 380 390 400
    LGGYRIPAGA DIIYSPYAIQ RDPKSYDDNL EFDPDRWLPE RAANVPKYAM
    410 420 430 440 450
    KPFSAGKRKC PSDHFSMAQL TLITAALATK YRFEQVAGSN DAVRVGITLR
    460
    PHDLLVRPVA R
    Length:461
    Mass (Da):50,938
    Last modified:October 1, 2000 - v1
    Checksum:i10282E36A405B57E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94608.1.
    RefSeqiNP_629370.1. NC_003888.3.
    WP_011030120.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB94608; CAB94608; CAB94608.
    GeneIDi1100664.
    KEGGisco:SCO5223.
    PATRICi23740298. VBIStrCoe124346_5307.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL939123 Genomic DNA. Translation: CAB94608.1.
    RefSeqiNP_629370.1. NC_003888.3.
    WP_011030120.1. NC_003888.3.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3DBGX-ray2.60A/B1-461[»]
    3EL3X-ray3.30A/B1-461[»]
    ProteinModelPortaliQ9K498.
    SMRiQ9K498.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi100226.SCO5223.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiCAB94608; CAB94608; CAB94608.
    GeneIDi1100664.
    KEGGisco:SCO5223.
    PATRICi23740298. VBIStrCoe124346_5307.

    Phylogenomic databases

    eggNOGiENOG41068W2. Bacteria.
    COG2124. LUCA.
    HOGENOMiHOG000043114.
    InParanoidiQ9K498.
    KOiK12645.
    OMAiEMSEWAS.
    OrthoDBiPOG091H09CE.
    PhylomeDBiQ9K498.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13905.

    Miscellaneous databases

    EvolutionaryTraceiQ9K498.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiEIZFM_STRCO
    AccessioniPrimary (citable) accession number: Q9K498
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: October 1, 2000
    Last modified: November 2, 2016
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.