Q9K3L9 (RLMG_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	SCO1041
ORF Names:	SCG20A.21

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.
Sequence caution	The sequence CAB96026.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA methylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366528

Sequences

	Sequence		Length	Mass (Da)
	Q9K3L9 [UniParc]. Last modified March 24, 2009. Version 2. Checksum: 4B0B9358A11F2419	FASTA	377	41,282
	10 20 30 40 50 60 MTTPWGERVL SRFPEDPRDR LRAWDASDEY LLGHLAEREV PLSGTVVVVG DRWGALVTAL 70 80 90 100 110 120 APHRPVQITD SHLAREATRV NLERSGVEPG SVRLLTTQDP PPDRVDVLLV RVPKSLALLE 130 140 150 160 170 180 DQLLRLAPAL HEGTVVVGTG MVKEIHTSTL RLFERIVGPT RTSLAVKKAR LIFAEPDPAL 190 200 210 220 230 240 KRPANPWPLG YRLPDDVGRL SGRPVVNHAG VFCADRLDIG TRFFLRHLPA PGRFRRVVDL 250 260 270 280 290 300 GCGNGVVGTA VSLADPDAEL LFTDESFQAV ASARATYRAN EVAGQAEFRV GDGLAGVPDG 310 320 330 340 350 360 SVDLVLNNPP FHSHQATTGA TAWRMFTGAR RVLRPGGELW VVGNRHLGYH VRLRRLFGNS 370 ELVAGDRKFV VLKAVKE

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939107 Genomic DNA. Translation: CAB96026.1. Different initiation.
RefSeq	NP_625336.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9K3L9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1096464.
GenomeReviews	Gene locus SCO1041 in contig AL645882_GR.
KEGG	sco:SCO1041.
NMPDR	fig|100226.1.peg.1008.
PATRIC	23731628. VBIStrCoe124346_1036.
Phylogenomic databases
HOGENOM	HBG321931.
PhylomeDB	Q9K3L9.
ProtClustDB	CLSK635276.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K00564.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
PRINTS	PR00508. S21N4MTFRASE.
ProtoNet	Search...

Entry information

Entry name

RLMG_STRCO

Accession

Primary (citable) accession number: Q9K3L9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	March 24, 2009
Last modified:	January 25, 2012
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program