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Protein

Malate dehydrogenase

Gene

mdh

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate. Exhibits remarkably higher catalytic efficiency for oxaloacetate reduction than for malate oxidation in vitro. Shows a high specificity for NAD(H), being almost inactive with NADP(H).1 Publication

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation1 Publication

Enzyme regulationi

Activity is inhibited by Zn2+ and Co2+. No activity in the presence of Fe2+.1 Publication

Kineticsi

kcat is 1870 sec(-1) with oxaloacetate as substrate. kcat is 542 sec(-1) with NADH as substrate. kcat is 4.71 sec(-1) with L-malate as substrate. kcat is 3.66 sec(-1) with NAD+ as substrate.1 Publication
  1. KM=0.189 mM for oxaloacetate1 Publication
  2. KM=0.083 mM for NADH1 Publication
  3. KM=0.494 mM for L-malate1 Publication
  4. KM=0.150 mM for NAD+1 Publication
  1. Vmax=1600 µmol/min/mg enzyme toward oxaloacetate1 Publication
  2. Vmax=464 µmol/min/mg enzyme toward NADH1 Publication
  3. Vmax=4.02 µmol/min/mg enzyme toward L-malate1 Publication
  4. Vmax=3.14 µmol/min/mg enzyme toward NAD+1 Publication

pH dependencei

Optimum pH is 6.8 at 50 degrees Celsius and 8.5 at 30 degrees Celsius.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei93SubstrateUniRule annotation1
Binding sitei99SubstrateUniRule annotation1
Binding sitei106NADUniRule annotation1
Binding sitei113NADUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Binding sitei163SubstrateUniRule annotation1
Active sitei188Proton acceptorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 18NADUniRule annotation7
Nucleotide bindingi130 – 132NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processTricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.37 5998

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase1 PublicationUniRule annotation (EC:1.1.1.37UniRule annotation1 Publication)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:SCO4827
ORF Names:SC2A6.12
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001133941 – 329Malate dehydrogenaseAdd BLAST329

Proteomic databases

PRIDEiQ9K3J3

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi100226.SCO4827

Structurei

3D structure databases

ProteinModelPortaliQ9K3J3
SMRiQ9K3J3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105D9Z Bacteria
COG0039 LUCA
HOGENOMiHOG000220953
InParanoidiQ9K3J3
KOiK00024
OMAiRPRTKGM
OrthoDBiPOG091H03R4
PhylomeDBiQ9K3J3

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_01517 Malate_dehydrog_2, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010945 Malate_DH_type2
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR23382 PTHR23382, 1 hit
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01759 MalateDH-SF1, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9K3J3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRTPVNVTV TGAAGQIGYA LLFRIASGQL LGADVPVKLR LLEITPALKA
60 70 80 90 100
AEGTAMELDD CAFPLLQGIE ITDDPNVAFD GANVALLVGA RPRTKGMERG
110 120 130 140 150
DLLEANGGIF KPQGKAINDH AADDIKVLVV GNPANTNALI AQAAAPDVPA
160 170 180 190 200
ERFTAMTRLD HNRALTQLAK KTGSTVADIK RLTIWGNHSA TQYPDIFHAT
210 220 230 240 250
VAGKNAAETV NDEKWLADEF IPTVAKRGAA IIEARGASSA ASAANAAIDH
260 270 280 290 300
VYTWVNGTAE GDWTSMGIPS DGSYGVPEGI ISSFPVTTKD GSYEIVQGLD
310 320
INEFSRARID ASVKELSEER EAVRGLGLI
Length:329
Mass (Da):34,643
Last modified:October 1, 2000 - v1
Checksum:i76E5F74769CCCAF4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939121 Genomic DNA Translation: CAB97430.1
RefSeqiNP_628983.1, NC_003888.3
WP_003974145.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB97430; CAB97430; CAB97430
GeneIDi1100268
KEGGisco:SCO4827
PATRICifig|100226.15.peg.4903

Similar proteinsi

Entry informationi

Entry nameiMDH_STRCO
AccessioniPrimary (citable) accession number: Q9K3J3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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