Q9K303 (BLAB3_FLAME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbapenem-hydrolyzing beta-lactamase BlaB-3
Short name=CHbetaL-3
EC=3.5.2.6

Alternative name(s):
Class B carbapenemase BlaB-3

Gene names

Name:	blaB3
Synonyms:	blaB

Organism

Flavobacterium meningosepticum (Chryseobacterium meningosepticum) (Elizabethkingia meningoseptica)

Taxonomic identifier

238 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Elizabethkingia

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes penicillins, cephalosporins (including cefoxitin), carbapenems and 6-beta-iodopenicillanate By similarity.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the class-B beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Gene Ontology (GO)
Biological_process	antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	beta-lactamase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 249	227	Carbapenem-hydrolyzing beta-lactamase BlaB-3		PRO_0000016951
Sites
	Metal binding	98	1	Zinc 1 By similarity
	Metal binding	100	1	Zinc 1 By similarity
	Metal binding	102	1	Zinc 2 By similarity
	Metal binding	161	1	Zinc 1 By similarity
	Metal binding	180	1	Zinc 2 By similarity
	Metal binding	222	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K303 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 65C437D44A143276	FASTA	249	28,044
	10 20 30 40 50 60 MLKKIKISLI LALGLTSLQA FGQENPDVKI EKLKDNLYVY TTYNTFNGTK YAANAVYLVT 70 80 90 100 110 120 DKGVVVIDCP WGEDKFKSFT DEIYKKHGKK VIINIATHSH DDRAGGLEYF GKIGAKTYST 130 140 150 160 170 180 KMTDSILAKE NKPRAQYTFD NNKSFKVGKS EFQVYYPGKG HTADNVVVWF PKEKVLVGGC 190 200 210 220 230 240 IIKSADSKDL GYIGEAYVND WTQSVHNIQQ KFSGAQYVVA GHDDWKDQTS IQHTLDLISE YQQKQKASN

References

[1]

"Molecular and biochemical heterogeneity of class B carbapenem-hydrolyzing beta-lactamases in Chryseobacterium meningosepticum." Bellais S., Aubert D., Naas T., Nordmann P. Antimicrob. Agents Chemother. 44:1878-1886(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13255 / CIP 6059 / CCUG 4321 / LMG 12873 / NCTC 10586 and GEO.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF189299 Genomic DNA. Translation: AAF89155.1. AF189301 Genomic DNA. Translation: AAF89157.1.
3D structure databases
ProteinModelPortal	Q9K303.
SMR	Q9K303. Positions 27-245.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.60.15.10. 1 hit.
InterPro	IPR001279. Beta-lactamas-like. IPR001018. Beta-lactamase_class-B_CS. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
PROSITE	PS00743. BETA_LACTAMASE_B_1. 1 hit. PS00744. BETA_LACTAMASE_B_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLAB3_FLAME

Accession

Primary (citable) accession number: Q9K303

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	May 29, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program