Q9K1J1 (RPOC_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	NMB0133

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	1391 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1391	1391	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067768

Sequences

	Sequence		Length	Mass (Da)
	Q9K1J1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 18FCEAE6CAC5D50E	FASTA	1,391	153,762
	10 20 30 40 50 60 MNLLNLFNPL QTAGMEEEFD AIKIGIASPE TIRSWSYGEV KKPETINYRT FKPERDGLFC 70 80 90 100 110 120 AKIFGPVKDY ECLCGKYKRL KFKGVTCEKC GVEVTLSKVR RERMGHIELA APVAHIWFLK 130 140 150 160 170 180 SLPSRLGMVL DMTLRDIERV LYFEAFVVTD PGMTPLQRRQ LLTEDDYYNK LDEYGDDFDA 190 200 210 220 230 240 KMGAEGIREL LRTLNVAGEI EILRQELEST GSDTKIKKIA KRLKVLEAFH RSGMKLEWMI 250 260 270 280 290 300 MDVLPVLPPD LRPLVPLDGG RFATSDLNDL YRRVINRNNR LKRLLELHAP DIIVRNEKRM 310 320 330 340 350 360 LQEAVDSLLD NGRRGKAMTG ANKRPLKSLA DMIKGKGGRF RQNLLGKRVD YSGRSVITVG 370 380 390 400 410 420 PYLRLHQCGL PKKMALELFK PFIFHKLEKQ GLASTVKAAK KLVEQEVPEV WDILEEVIRE 430 440 450 460 470 480 HPIMLNRAPT LHRLGIQAFE PILIEGKAIQ LHPLVCAAFN ADFDGDQMAV HVPLSLEAQM 490 500 510 520 530 540 EARTLMLASN NVLSPANGEP IIVPSQDIVL GLYYMTRDRI NAKGEGSLFA DVKEVHRAYH 550 560 570 580 590 600 TKQVELGTKI TVRLREWVKN EAGEFEPVVN RYETTVGRAL LSEILPKGLP FEYVNKALKK 610 620 630 640 650 660 KEISKLINAS FRLCGLRDTV IFADHLMYTG FGFAAKGGIS IAVDDMEIPK EKAALLAEAN 670 680 690 700 710 720 AEVKEIEDQY RQGLVTNGER YNKVVDIWGR AGDKIAKAMM DNLSKQKVID RAGNEVDQES 730 740 750 760 770 780 FNSIYMMADS GARGSAAQIK QLSGMRGLMA KPDGSIIETP ITSNFREGLT VLQYFIATHG 790 800 810 820 830 840 ARKGLADTAL KTANSGYLTR RLVDVTQDLV VVEDDCGTSD GFVMKAVVQG GDVIEALRDR 850 860 870 880 890 900 ILGRVTASDV VDPSSGETLV EAGTLLTEKL VDMIDQSGVD EVKVRTPITC KTRHGLCAHC 910 920 930 940 950 960 YGRDLARGKL VNAGEAVGVI AAQSIGEPGT QLTMRTFHIG GAASRAAAAS QVEAKSNGTA 970 980 990 1000 1010 1020 RFSSQMRYVA NNKGELVVIG RSCEVVIHDD IGRERERHKV PYGAILLVQD GMAIKAGQTL 1030 1040 1050 1060 1070 1080 ATWDPHTRPM ITEHAGMVKF ENVEEGVTVA KQTDDVTGLS TLVVIDGKRR SSSASKLLRP 1090 1100 1110 1120 1130 1140 TVKLLDENGV EICIPGTSTP VSMAFPVGAV ITVREGQEIG KGDVLARIPQ ASSKTRDITG 1150 1160 1170 1180 1190 1200 GLPRVAELFE ARVPKDAGML AEITGTVSFG KETKGKQRLI VTDVDGVAYE TLISKEKQIL 1210 1220 1230 1240 1250 1260 VHDGQVVNRG ETIVDGAVDP HDILRLQGIE ALARYIVQEV QEVYRLQGVK ISDKHIEVII 1270 1280 1290 1300 1310 1320 RQMLRRVNIA DAGETGFITG EQVERGDVMA ANEKALEEGK EPARYENVLL GITKASLSTD 1330 1340 1350 1360 1370 1380 SFISAASFQE TTRVLTEAAI MGKQDELRGL KENVIVGRLI PAGTGLTYHR SRHQQWQEVE 1390 QETAETQVTD E

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40592.1.
PIR	F81233.
RefSeq	NP_273191.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K1J1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009428; EBNEIP00000009048; EBNEIG00000009428.
GeneID	902241.
GenomeReviews	Gene locus NMB0133 in contig AE002098_GR.
KEGG	nme:NMB0133.
NMPDR	fig|122586.1.peg.125.
PATRIC	20355287. VBINeiMen85645_0173.
TIGR	NMB0133.
Phylogenomic databases
GeneTree	EBGT00050000020766.
HOGENOM	HBG621785.
OMA	DINDKHI.
ProtClustDB	PRK00566.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0133-MONOMER.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RPOC_NEIMB

Accession

Primary (citable) accession number: Q9K1J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program