Q9K1F9 (PDXA_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxythreonine-4-phosphate dehydrogenase
EC=1.1.1.262

Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase

Gene names

Name:	pdxA
Ordered Locus Names:	NMB0195

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP MF_00536
Catalytic activity	4-(phosphonooxy)-L-threonine + NAD+ = (2S)-2-amino-3-oxo-4-phosphonooxybutanoate + NADH. HAMAP MF_00536
Cofactor	Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.
Pathway	Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP MF_00536
Subunit structure	Homodimer By similarity. HAMAP MF_00536
Subcellular location	Cytoplasm By similarity HAMAP MF_00536.
Miscellaneous	The active site is located at the dimer interface By similarity. HAMAP MF_00536
Sequence similarities	Belongs to the pdxA family.
Sequence caution	The sequence AAF40652.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Pyridoxine biosynthesis
Cellular component	Cytoplasm
Ligand	Cobalt Magnesium Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-hydroxythreonine-4-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	4-hydroxythreonine-4-phosphate dehydrogenase HAMAP MF_00536		PRO_0000188812
Sites
	Metal binding	166	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	211	1	Divalent metal cation; shared with dimeric partner By similarity
	Metal binding	266	1	Divalent metal cation; shared with dimeric partner By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	274	1	Substrate By similarity
	Binding site	283	1	Substrate By similarity
	Binding site	292	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K1F9 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: 0126A0A7D7B16DDC	FASTA	329	34,505
	10 20 30 40 50 60 MKQPVFAVTS GEPAGIGPDI CLDLAFARLP CRCAVLGDKN LLRARAEALG KSVVLRDFDP 70 80 90 100 110 120 ESGGAAYGEL EVLHIPAVEA VEAGKLNPAN AAYVLQLLDT ALAGISDGIF DGIVTAPLHK 130 140 150 160 170 180 GIINDARAST GFFSGHTEYL AEKSGTGQVV MMLAGKGLRV ALVTTHLPLK DVAAAITQPL 190 200 210 220 230 240 IESVARILHH DLKHKFGIKN PKILVAGLNP HAGEGGHLGH EETDTIIPAL ENLRREGINL 250 260 270 280 290 300 AGPYPADTLF QPFMLEGADA VLAMYHDQGL PVLKYHSFGQ GVNITLGLPF IRTSVDHGTA 310 320 LDLAATGRAD SGSLITAVET AVEMARGSL

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40652.1. Different initiation.
PIR	H81227.
RefSeq	NP_273253.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K1F9.
SMR	Q9K1F9. Positions 1-327.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009208; EBNEIP00000008828; EBNEIG00000009208.
GeneID	902303.
GenomeReviews	Gene locus NMB0195 in contig AE002098_GR.
KEGG	nme:NMB0195.
NMPDR	fig|122586.1.peg.187.
PATRIC	20355423. VBINeiMen85645_0240.
TIGR	NMB0195.
Phylogenomic databases
GeneTree	EBGT00050000021408.
HOGENOM	HBG451136.
OMA	INLAGPY.
ProtClustDB	PRK00232.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0195-MONOMER.
Family and domain databases
HAMAP	MF_00536. PdxA. [Tree]
InterPro	IPR005255. PyrdxlP_synth_PdxA. [Graphical view]
KO	K00097.
Pfam	PF04166. PdxA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00557. PdxA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PDXA_NEIMB

Accession

Primary (citable) accession number: Q9K1F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	January 25, 2012
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program