Q9K1B4 (NUOH_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit H
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit H
NDH-1 subunit H

Gene names

Name:	nuoH
Ordered Locus Names:	NMB0250

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient. This subunit may bind ubiquinone By similarity. HAMAP MF_01350
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01350
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential HAMAP MF_01350.
Sequence similarities	Belongs to the complex I subunit 1 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADH dehydrogenase (quinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	NADH-quinone oxidoreductase subunit H HAMAP MF_01350		PRO_0000240088
Regions
	Transmembrane	20 – 40	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	128 – 148	21	Helical; Potential
	Transmembrane	168 – 188	21	Helical; Potential
	Transmembrane	206 – 226	21	Helical; Potential
	Transmembrane	253 – 273	21	Helical; Potential
	Transmembrane	295 – 315	21	Helical; Potential
	Transmembrane	334 – 354	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K1B4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 26C6B7881C64EF66	FASTA	358	39,851
	10 20 30 40 50 60 MQEWFQNLFA ATLGLGDLGI TVGLVVSVIV KIVIILIPLI LTVAYLTYFE RKVIGFMQLR 70 80 90 100 110 120 VGPNVTGPWG LIQPFADVFK LLFKEVTRPK LSNKALFYIG PIMSLAPSFA AWAVIPFNEE 130 140 150 160 170 180 WVLTNINIGL LYILMITSLS VYGVIIAGWA SNSKYSFLGA MRASAQSISY EIAMSAALVC 190 200 210 220 230 240 VVMVSGSMNF SDIVAAQAKG IAGGSVFSWN WLPLFPIFIV YLISAVAETN RAPFDVAEGE 250 260 270 280 290 300 SEIVAGHHVE YSGFAFALFF LAEYIFMILI AALTSLMFLG GWLSPFPQSW GIVGTPSAFW 310 320 330 340 350 MFAKMAAVLY WYLWIRATFP RYRYDQIMRL GWKVLIPIGF AYIVILGVWM ISPLNLWK

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40704.1.
PIR	E81219.
RefSeq	NP_273306.1. NC_003112.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009482; EBNEIP00000009102; EBNEIG00000009482.
GeneID	902361.
GenomeReviews	Gene locus NMB0250 in contig AE002098_GR.
KEGG	nme:NMB0250.
NMPDR	fig|122586.1.peg.240.
PATRIC	20355578. VBINeiMen85645_0313.
TIGR	NMB0250.
Phylogenomic databases
GeneTree	EBGT00050000020449.
HOGENOM	HBG727670.
OMA	YLWVRAT.
ProtClustDB	CLSK877460.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0250-MONOMER.
Family and domain databases
HAMAP	MF_01350. NDH1_NuoH. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1/FPO. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
KO	K00337.
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. False negative. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOH_NEIMB

Accession

Primary (citable) accession number: Q9K1B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program