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Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathway: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
Binding sitei95 – 951Substrate; via amide nitrogen and carbonyl oxygenBy similarity
Active sitei147 – 1471Proton acceptorBy similarity
Active sitei157 – 1571Proton acceptorBy similarity
Binding sitei164 – 1641NADBy similarity
Sitei206 – 2061Involved in acyl-ACP bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NADBy similarity
Nucleotide bindingi64 – 652NADBy similarity
Nucleotide bindingi193 – 1975NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciNMEN122586:GHGG-357-MONOMER.
UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:NMB0336
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000425 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 261261Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000320269Add
BLAST

Proteomic databases

PRIDEiQ9K151.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi122586.NMB0336.

Structurei

3D structure databases

ProteinModelPortaliQ9K151.
SMRiQ9K151. Positions 2-258.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiRQFRLTQ.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.

Sequencei

Sequence statusi: Complete.

Q9K151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLQGKKIL ITGMISERSI AYGIAKACRE QGAELAFTYV VDKLEERVRK
60 70 80 90 100
MAAELDSELV FRCDVASDDE INQVFADLGK HWDGLDGLVH SIGFAPKEAL
110 120 130 140 150
SGDFLDSISR EAFNTAHEIS AYSLPALAKA ARPMMRGRNS AIVALSYLGA
160 170 180 190 200
VRAIPNYNVM GMAKASLEAG IRFTAACLGK EGIRCNGISA GPIKTLAASG
210 220 230 240 250
IADFGKLLGH VAAHNPLRRN VTIEEVGNTA AFLLSDLSSG ITGEITYVDG
260
GYSINALSTE G
Length:261
Mass (Da):27,691
Last modified:October 1, 2000 - v1
Checksum:iAF2D0C70F550253D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA. Translation: AAF40779.1.
PIRiC81211.
RefSeqiNP_273385.1. NC_003112.2.
WP_002223367.1. NC_003112.2.

Genome annotation databases

EnsemblBacteriaiAAF40779; AAF40779; NMB0336.
GeneIDi902451.
KEGGinme:NMB0336.
PATRICi20355813. VBINeiMen85645_0425.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA. Translation: AAF40779.1.
PIRiC81211.
RefSeqiNP_273385.1. NC_003112.2.
WP_002223367.1. NC_003112.2.

3D structure databases

ProteinModelPortaliQ9K151.
SMRiQ9K151. Positions 2-258.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi122586.NMB0336.

Proteomic databases

PRIDEiQ9K151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF40779; AAF40779; NMB0336.
GeneIDi902451.
KEGGinme:NMB0336.
PATRICi20355813. VBINeiMen85645_0425.

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiRQFRLTQ.
OrthoDBiEOG6HF644.

Enzyme and pathway databases

UniPathwayiUPA00094.
BioCyciNMEN122586:GHGG-357-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
PRINTSiPR00081. GDHRDH.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC58.
  2. "Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254."
    Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.
    Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: NZ98/254 / Serogroup B.

Entry informationi

Entry nameiFABI_NEIMB
AccessioniPrimary (citable) accession number: Q9K151
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Present in outer membrane vesicle formulations which are used as vaccines in human.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.