Reviewed, UniProtKB/Swiss-Prot Q9K107 (NADB_NEIMB)

Names and origin

Protein names

Recommended name:
    L-aspartate oxidase
      Short name=LASPO
    EC=1.4.3.16
Alternative name(s):
    Quinolinate synthetase B

Gene names

Name:	nadB
Ordered Locus Names:	NMB0392

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of L-aspartate to iminoaspartate.
Catalytic activity	L-aspartate + O2 = iminosuccinate + H2O2.
Cofactor	FAD.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (oxidase route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-aspartate oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	L-aspartate oxidase		PRO_0000184392
Regions
	Nucleotide binding	8 – 22	15	FAD Potential
Sites
	Active site	228	1	By similarity
	Active site	249	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K107-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 644152CC3753E294	FASTA	502	54,563
	10 20 30 40 50 60 MQTDCDVLIA GNGLAALTLA LSLPESFRIV ILCKNRLDDT ASRHAQGGIA AAWSGEDDIE 70 80 90 100 110 120 KHVADTLEAG AGLCDEAAVR AILSQGKPAI EWLLAQGVAF DRNHNGLHLT REGGHTCRRI 130 140 150 160 170 180 AHVADYTGEA VMQSLIAQIR RRPNIRVCER QMALDIQTES GAACGLTVLD CRTQETYRIR 190 200 210 220 230 240 ARHTVLAGGG LGQIYAATTT PPECTGDAIA MAIRAGCAVG NLEFIQFHPT GLARPSENGR 250 260 270 280 290 300 TFLISEAVRG EGGILTNQAG ERFMPHYDRR AELAPRDIVA RAIAAEIAKQ TQDFVSLDIS 310 320 330 340 350 360 HQPAAFVRRH FPSIHRHCLS QCGLDITRQA IPVRPVQHYT CGGIQTDPCG RTSLPQLYAL 370 380 390 400 410 420 GETACTGLHG ANRLASNSLL ECVVTARLCA QAIADGQAFQ AEPFQRPSES LSAEAGIFSD 430 440 450 460 470 480 DLQNTFSRPV LQTFNQRHLG ILRNDTGLRR AIAQLQLWKQ NQAEPHTASE YENRNLLECS 490 500 LAVAQAAYRR RQNIGAHFNS DC

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40832.1.
PIR	H81203.
RefSeq	NP_273441.1.
3D structure databases
SMR	Q9K107. Positions 3-502.
ModBase	Search...
Genome annotation databases
GeneID	902507.
GenomeReviews	Gene locus NMB0392 in contig AE002098_GR.
KEGG	nme:NMB0392.
NMPDR	fig|122586.1.peg.375.
TIGR	NMB0392.
Phylogenomic databases
HOGENOM	HBG293998.
OMA	GAYIWNR.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0392-MONOMER.
BRENDA	1.4.3.16. 293828.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR015939. Fum_Rdtase/Succ_DH_flav-like_C. IPR004112. Fum_Rdtase/Succ_DH_flav_C. IPR005288. NadB. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00551. nadB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADB_NEIMB

Accession

Primary (citable) accession number: Q9K107

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)