Q9K0Y4 (MURD_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	NMB0420

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	445 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 445	445	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000109049
Regions
	Nucleotide binding	117 – 123	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K0Y4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9B30F5F67A060393	FASTA	445	48,163
	10 20 30 40 50 60 MTFQNKKILV AGLGGTGISM IAYLRKNGAE VAAYDAELKP ERVSQIGKMF DGLVFYTGRL 70 80 90 100 110 120 KDALDNGFDI LALSPGISER QPDIEAFKQN GGRVLGDIEL LADIVNRRDD KVIAITGSNG 130 140 150 160 170 180 KTTVTSLVGY LCIKCGLDTV IAGNIGTPVL EAEWQREGKK ADVWVLELSS FQLENTESLR 190 200 210 220 230 240 PTAATVLNIS EDHLDRYDDL LDYAHTKAKI FRGDGVQVLN ADDAFCRAMK RAGREVKWFS 250 260 270 280 290 300 LEHEADFWLE RETGRLKQGN EDLIVTQDIP LQGLHNAANV MAAVALCEAI GLSREALLEH 310 320 330 340 350 360 VKTFQGLPHR VEKIGEKNGV VFIDDSKGTN VGATAAAIAG LQNPLFVILG GMGKGQDFTP 370 380 390 400 410 420 LRDALVGKAK GVFLIGVDAP QIRRDLDGCG LNMTDCATLG EAVQTAYAQA EAGDIVLLSP 430 440 ACASFDMFKG YAHRSEVFIE AFKAL

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40858.1.
PIR	G81200.
RefSeq	NP_273468.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K0Y4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000010373; EBNEIP00000009993; EBNEIG00000010373.
GeneID	902536.
GenomeReviews	Gene locus NMB0420 in contig AE002098_GR.
KEGG	nme:NMB0420.
NMPDR	fig|122586.1.peg.402.
PATRIC	20356034. VBINeiMen85645_0532.
TIGR	NMB0420.
Phylogenomic databases
GeneTree	EBGT00050000020542.
HOGENOM	HBG750024.
OMA	ACASWDM.
ProtClustDB	PRK04308.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0420-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_NEIMB

Accession

Primary (citable) accession number: Q9K0Y4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program