Reviewed, UniProtKB/Swiss-Prot Q9K0V3 (AMIC_NEIMB)

Names and origin

Protein names

Recommended name:
    N-acetylmuramoyl-L-alanine amidase amiC
    EC=3.5.1.28

Gene names

Name:	amiC
Ordered Locus Names:	NMB0456

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cell-wall hydrolase probably involved in cell-wall hydrolysis, septation or recycling By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Miscellaneous	Present in outer membrane vesicle formulations which are used as vaccines in human.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 416	390	N-acetylmuramoyl-L-alanine amidase amiC		PRO_0000320265

Sequences

	Sequence		Length	Mass (Da)
	Q9K0V3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5E89E0A60DE8AD21	FASTA	416	45,190
	10 20 30 40 50 60 MIKLTRRQII RRTAGTLFAL SPIASAVAKT VRAPQFTAAR IWPSHTYTRL TLESTAALKY 70 80 90 100 110 120 QHFTLDNPGR LVVDIQNANI NTVLHGLSQK VMADDPFIRS IRAGQNTPTT VRLVIDLKQP 130 140 150 160 170 180 THAQVFALPP VGGFKNRLVV DLYPHGMDAD DPMMALLNGS LNKTLRGSPE ADLAQNTTPQ 190 200 210 220 230 240 PGRGRNGRRP VIMLDPGHGG EDPGAISPGG LQEKHVVLSI ARETKNQLEA LGYNVFMTRN 250 260 270 280 290 300 EDVFIPLGVR VAKGRARRAD VFVSIHADAF TSPSARGTGV YMLNTKGATS SAAKFLEQTQ 310 320 330 340 350 360 NNADAVGGVP TSGNRNVDTA LLDMTQTATL RDSRKLGKLV LEELGRLNHL HKGRVDEANF 370 380 390 400 410 AVLRAPDMPS ILVETAFLSN PAEEKLLGSE SFRRQCAQSI ASGVQRYINT SVLKRG

References

[1]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.
[2]	"Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry." Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J. Hum. Vaccin. 1:80-84(2005) [PubMed: 17038831] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[3]	"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed: 16645985] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40893.1.
PIR	B81198.
RefSeq	NP_273503.1.
3D structure databases
SMR	Q9K0V3. Positions 190-410.
ModBase	Search...
Genome annotation databases
GeneID	902572.
GenomeReviews	Gene locus NMB0456 in contig AE002098_GR.
KEGG	nme:NMB0456.
NMPDR	fig|122586.1.peg.437.
TIGR	NMB0456.
Phylogenomic databases
HOGENOM	HBG656726.
OMA	LWRAPDN.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0456-MONOMER.
BRENDA	3.5.1.28. 293828.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIC_NEIMB

Accession

Primary (citable) accession number: Q9K0V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)