Q9K0V3 (AMIC_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylmuramoyl-L-alanine amidase AmiC
EC=3.5.1.28

Gene names

Name:	amiC
Ordered Locus Names:	NMB0456

Organism

Neisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]

Taxonomic identifier

122586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup B

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cell-wall hydrolase involved in septum cleavage during cell division By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Periplasm By similarity.
Miscellaneous	Present in outer membrane vesicle formulations which are used as vaccines in human.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Periplasm
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 416	390	N-acetylmuramoyl-L-alanine amidase AmiC		PRO_0000320265

Sequences

	Sequence		Length	Mass (Da)
	Q9K0V3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5E89E0A60DE8AD21	FASTA	416	45,190
	10 20 30 40 50 60 MIKLTRRQII RRTAGTLFAL SPIASAVAKT VRAPQFTAAR IWPSHTYTRL TLESTAALKY 70 80 90 100 110 120 QHFTLDNPGR LVVDIQNANI NTVLHGLSQK VMADDPFIRS IRAGQNTPTT VRLVIDLKQP 130 140 150 160 170 180 THAQVFALPP VGGFKNRLVV DLYPHGMDAD DPMMALLNGS LNKTLRGSPE ADLAQNTTPQ 190 200 210 220 230 240 PGRGRNGRRP VIMLDPGHGG EDPGAISPGG LQEKHVVLSI ARETKNQLEA LGYNVFMTRN 250 260 270 280 290 300 EDVFIPLGVR VAKGRARRAD VFVSIHADAF TSPSARGTGV YMLNTKGATS SAAKFLEQTQ 310 320 330 340 350 360 NNADAVGGVP TSGNRNVDTA LLDMTQTATL RDSRKLGKLV LEELGRLNHL HKGRVDEANF 370 380 390 400 410 AVLRAPDMPS ILVETAFLSN PAEEKLLGSE SFRRQCAQSI ASGVQRYINT SVLKRG

References

[1]	"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58.
[2]	"Characterization of the protein content of a meningococcal outer membrane vesicle vaccine by polyacrylamide gel electrophoresis and mass spectrometry." Vipond C., Wheeler J.X., Jones C., Feavers I.M., Suker J. Hum. Vaccin. 1:80-84(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[3]	"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: NZ98/254 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF40893.1.
PIR	B81198.
RefSeq	NP_273503.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K0V3.
ModBase	Search...
Protein-protein interaction databases
STRING	122586.NMB0456.
Protocols and materials databases
DNASU	902572.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF40893; AAF40893; NMB0456.
GeneID	902572.
KEGG	nme:NMB0456.
PATRIC	20356126. VBINeiMen85645_0579.
Phylogenomic databases
eggNOG	COG0860.
HOGENOM	HOG000263827.
KO	K01448.
OMA	ENESELM.
ProtClustDB	CLSK877567.
Enzyme and pathway databases
BioCyc	NMEN122586:GHGG-457-MONOMER.
Family and domain databases
Gene3D	3.40.630.40. 2 hits.
InterPro	IPR021731. AMIN_dom. IPR002508. CW_Hdrlase/autolysin_cat. [Graphical view]
Pfam	PF01520. Amidase_3. 1 hit. PF11741. AMIN. 1 hit. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIC_NEIMB

Accession

Primary (citable) accession number: Q9K0V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program