Q9K0H4 (HIS6_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Imidazole glycerol phosphate synthase subunit HisF
EC=4.1.3.-

Alternative name(s):
IGP synthase cyclase subunit
IGP synthase subunit HisF
ImGP synthase subunit HisF
Short name=IGPS subunit HisF

Gene names

Name:	hisF
Ordered Locus Names:	NMB0628

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit By similarity. HAMAP MF_01013
Catalytic activity	5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4-carboxamide + L-glutamine = imidazole-glycerol phosphate + 5-aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H2O. HAMAP MF_01013
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. HAMAP MF_01013
Subunit structure	Heterodimer of HisH and HisF By similarity. HAMAP MF_01013
Subcellular location	Cytoplasm By similarity HAMAP MF_01013.
Sequence similarities	Belongs to the HisA/HisF family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	histidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazoleglycerol-phosphate synthase activity Inferred from electronic annotation. Source: InterPro lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Imidazole glycerol phosphate synthase subunit HisF HAMAP MF_01013		PRO_0000142188
Sites
	Active site	12	1	Potential
	Active site	131	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K0H4 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EAABB234BF30E23C	FASTA	255	27,001
	10 20 30 40 50 60 MALAKRIIPC LDVKDGRVVK GVNFIGLRDA GDPVEAAKRY NGEGADELTF LDITASSDNR 70 80 90 100 110 120 DTILHIIEEV AGQVFIPLTV GGGVRTVADI RRLLNAGADK VSINTAAVTR PDLIDEAAGF 130 140 150 160 170 180 FGSQAIVAAV DAKAANPENT RWEIFTHGGR NPTGLDAVEW AVEMQKRGAG EILLTGMDRD 190 200 210 220 230 240 GTKQGFNLPL TRAVAEAVDI PVIASGGVGN VRHLIEGITE GKADAVLAAG IFHFGEIAIR 250 EAKRAMREAG IEVRL

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF41053.1.
PIR	H81176.
RefSeq	NP_273671.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K0H4.
SMR	Q9K0H4. Positions 2-255.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009147; EBNEIP00000008767; EBNEIG00000009147.
GeneID	902741.
GenomeReviews	Gene locus NMB0628 in contig AE002098_GR.
KEGG	nme:NMB0628.
NMPDR	fig|122586.1.peg.605.
PATRIC	20356553. VBINeiMen85645_0796.
TIGR	NMB0628.
Phylogenomic databases
GeneTree	EBGT00050000020429.
HOGENOM	HBG541613.
OMA	RVVKGTN.
ProtClustDB	PRK02083.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0628-MONOMER.
Family and domain databases
HAMAP	MF_01013. HisF. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR004651. HisF. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K02500.
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00735. HisF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS6_NEIMB

Accession

Primary (citable) accession number: Q9K0H4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program