Q9K085 (BIOD_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	NMB0733

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187979
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
	Nucleotide binding	115 – 118	4	ATP By similarity
	Nucleotide binding	175 – 176	2	ATP By similarity
Sites
	Metal binding	13	1	Magnesium 1 By similarity
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	50	1	Magnesium 2 By similarity
	Metal binding	115	1	Magnesium 2 By similarity
	Binding site	42	1	Substrate By similarity
	Binding site	50	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9K085 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E1F98EB99D5DEE90	FASTA	215	23,701
	10 20 30 40 50 60 MKGVYFVSGI DTDIGKTVAT GVLAKQLLQQ GKSVITQKPV QTGCQNIADD IAVHRKIMGI 70 80 90 100 110 120 PMQEADKRRL TMPEIFSYPA SPHLAARLDG RALDLDKIRT ATQELAAQYE VVLVEGAGGL 130 140 150 160 170 180 MVPLTENLLT IDYIRQQGYP VILVTSGRLG SINHTLLSFA ALKQYGIRLH SLVFNHIHDS 190 200 210 RDAHIAQDSL SYLKCRLKAD FSEAEWMELA KTDAV

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF41146.1.
PIR	E81164.
RefSeq	NP_273775.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K085.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000010008; EBNEIP00000009628; EBNEIG00000010008.
GeneID	902846.
GenomeReviews	Gene locus NMB0733 in contig AE002098_GR.
KEGG	nme:NMB0733.
NMPDR	fig|122586.1.peg.709.
PATRIC	20356825. VBINeiMen85645_0933.
TIGR	NMB0733.
Phylogenomic databases
GeneTree	EBGT00050000021719.
HOGENOM	HBG650065.
OMA	IDTGIGK.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0733-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_NEIMB

Accession

Primary (citable) accession number: Q9K085

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program