Q9K081 (HPRK_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HPr kinase/phosphorylase
Short name=HPrK/P
EC=2.7.11.-
EC=2.7.4.-

Alternative name(s):
HPr(Ser) kinase/phosphorylase

Gene names

Name:	hprK
Ordered Locus Names:	NMB0737

Organism

Neisseria meningitidis serogroup B

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ATP- as well as the pyrophosphate-dependent phosphorylation of a specific serine residue in HPr, a phosphocarrier protein of the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS). HprK/P also catalyzes the pyrophosphate-producing, inorganic phosphate-dependent dephosphorylation (phosphorolysis) of seryl-phosphorylated HPr (P-Ser-HPr) By similarity. HAMAP MF_01249
Catalytic activity	ATP + HPr = ADP + P-Ser-HPr. HAMAP MF_01249 P-Ser-HPr + phosphate = HPr + diphosphate. HAMAP MF_01249
Cofactor	Magnesium By similarity. HAMAP MF_01249
Subunit structure	Homohexamer By similarity. HAMAP MF_01249
Domain	The Walker A ATP-binding motif also binds Pi and PPi By similarity. HAMAP MF_01249
Miscellaneous	Both phosphorylation and phosphorolysis are carried out by the same active site and suggest a common mechanism for both reactions By similarity. HAMAP MF_01249
Sequence similarities	Belongs to the HPrK/P family.

Ontologies

Keywords
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	regulation of carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	HPr kinase/phosphorylase HAMAP MF_01249		PRO_0000058976
Regions
	Nucleotide binding	156 – 163	8	ATP By similarity
	Region	204 – 213	10	Important for the catalytic mechanism of both phosphorylation and dephosphorylation By similarity
	Region	269 – 274	6	Important for the catalytic mechanism of dephosphorylation By similarity
Sites
	Active site	141	1	By similarity
	Active site	162	1	By similarity
	Active site	180	1	Proton acceptor; for phosphorylation activity. Proton donor; for dephosphorylation activity By similarity
	Active site	248	1	By similarity
	Metal binding	163	1	Magnesium Potential
	Metal binding	205	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9K081 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F57DED8C8DD02A13	FASTA	320	35,800
	10 20 30 40 50 60 MPSISVRRLF DDNQYKLQLA WAAGNSGADN RIGVEADKPV LALVGHLNFI HPNQIQVVGL 70 80 90 100 110 120 AESEYLNRLE SGETGYQFGD LFDISMSLVI VANGLPVSPG LRDYCHKNDI PLLTSKLESP 130 140 150 160 170 180 YLMDVLRIYL QRTLAASSVK HGVFLDVFEI GVLITGHSGL GKSELALELI SRGHSLIADD 190 200 210 220 230 240 AVELFRIGPE TLEGRCSPML RDFLEVRGLG ILNIRHIFGE TSIRPKKILQ LIINLVEADD 250 260 270 280 290 300 EYMKQLDRLS IRTETESILN VNVRSVTLPV AVGRNLAVLV EAAVRNYILQ LRGKDSTREF 310 320 LERHQTQLKE NEQHNEDRPD

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF41150.1.
PIR	A81165.
RefSeq	NP_273779.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9K081.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBNEIT00000009083; EBNEIP00000008703; EBNEIG00000009083.
GeneID	902850.
GenomeReviews	Gene locus NMB0737 in contig AE002098_GR.
KEGG	nme:NMB0737.
PATRIC	20356833. VBINeiMen85645_0937.
TIGR	NMB0737.
Phylogenomic databases
GeneTree	EBGT00050000020223.
HOGENOM	HBG566466.
OMA	GIGKSEC.
ProtClustDB	PRK05428.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_0737-MONOMER.
Family and domain databases
HAMAP	MF_01249. HPr_kinase. [Tree]
InterPro	IPR003755. Sig_transdc_Ser_kin/Pase_Hpr. IPR011104. Sig_transdc_Ser_kin/Pase_Hpr_C. IPR011126. Sig_transdc_Ser_kin/Pase_Hpr_N. [Graphical view]
KO	K06023.
Pfam	PF07475. Hpr_kinase_C. 1 hit. PF02603. Hpr_kinase_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HPRK_NEIMB

Accession

Primary (citable) accession number: Q9K081

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program