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Q9JZQ3

- METF_NEIMB

UniProt

Q9JZQ3 - METF_NEIMB

Protein

5,10-methylenetetrahydrofolate reductase

Gene

metF

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

    Cofactori

    FAD.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei26 – 261Proton donor/acceptorBy similarity
    Binding sitei86 – 861FADBy similarity
    Binding sitei118 – 1181SubstrateBy similarity
    Binding sitei150 – 1501FADBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei217 – 2171SubstrateBy similarity
    Binding sitei273 – 2731SubstrateBy similarity
    Binding sitei277 – 2771SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 316NADBy similarity
    Nucleotide bindingi57 – 604FADBy similarity
    Nucleotide bindingi57 – 582NADBy similarity
    Nucleotide bindingi116 – 1183FADBy similarity
    Nucleotide bindingi129 – 1302FADBy similarity
    Nucleotide bindingi154 – 1574FADBy similarity
    Nucleotide bindingi163 – 1708FADBy similarity

    GO - Molecular functioni

    1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

    GO - Biological processi

    1. methionine biosynthetic process Source: UniProtKB-KW
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Methionine biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein, NAD, NADP

    Enzyme and pathway databases

    BioCyciNMEN122586:GHGG-980-MONOMER.
    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
    Gene namesi
    Name:metF
    Ordered Locus Names:NMB0943
    OrganismiNeisseria meningitidis serogroup B (strain MC58)
    Taxonomic identifieri122586 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
    ProteomesiUP000000425: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2922925,10-methylenetetrahydrofolate reductasePRO_0000320271Add
    BLAST

    Proteomic databases

    PRIDEiQ9JZQ3.

    Interactioni

    Protein-protein interaction databases

    STRINGi122586.NMB0943.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JZQ3.
    SMRiQ9JZQ3. Positions 3-292.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0685.
    HOGENOMiHOG000246232.
    KOiK00297.
    OMAiLAMTHIL.
    OrthoDBiEOG6D2KVW.

    Family and domain databases

    Gene3Di3.20.20.220. 1 hit.
    InterProiIPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view]
    PfamiPF02219. MTHFR. 1 hit.
    [Graphical view]
    SUPFAMiSSF51730. SSF51730. 1 hit.
    TIGRFAMsiTIGR00676. fadh2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9JZQ3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNYAKEINAL NNSLSDLKGD INVSFEFFPP KNEQMETMLW DSIHRLQTLH    50
    PKFVSVTYGA NSGERDRTHG IVKRIKQETG LEAAPHLTGI DASPDELRQI 100
    AKDYWDSGIR RIVALRGDEP AGYEKKPFYA EDLVKLLRSV ADFDISVAAY 150
    PEVHPEAKSA QADLINLKRK IDAGANHVIT QFFFDVERYL RFRDRCVMLG 200
    IDVEIVPGIL PVTNFKQLGK MAQVTNVKIP SWLSQMYEGL DDDQGTRNLV 250
    AASIAIDMVK VLSREGVKDF HFYTLNRSEL TYAICHILGV RP 292
    Length:292
    Mass (Da):33,056
    Last modified:October 1, 2000 - v1
    Checksum:i32B22282A681CB1F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE002098 Genomic DNA. Translation: AAF41349.1.
    PIRiD81140.
    RefSeqiNP_273981.1. NC_003112.2.

    Genome annotation databases

    EnsemblBacteriaiAAF41349; AAF41349; NMB0943.
    GeneIDi903063.
    KEGGinme:NMB0943.
    PATRICi20357367. VBINeiMen85645_1198.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE002098 Genomic DNA. Translation: AAF41349.1 .
    PIRi D81140.
    RefSeqi NP_273981.1. NC_003112.2.

    3D structure databases

    ProteinModelPortali Q9JZQ3.
    SMRi Q9JZQ3. Positions 3-292.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 122586.NMB0943.

    Proteomic databases

    PRIDEi Q9JZQ3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF41349 ; AAF41349 ; NMB0943 .
    GeneIDi 903063.
    KEGGi nme:NMB0943.
    PATRICi 20357367. VBINeiMen85645_1198.

    Phylogenomic databases

    eggNOGi COG0685.
    HOGENOMi HOG000246232.
    KOi K00297.
    OMAi LAMTHIL.
    OrthoDBi EOG6D2KVW.

    Enzyme and pathway databases

    UniPathwayi UPA00193 .
    BioCyci NMEN122586:GHGG-980-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.220. 1 hit.
    InterProi IPR029041. FAD-linked_oxidoreductase-like.
    IPR003171. Mehydrof_redctse.
    IPR004620. MTHF_reductase_bac.
    [Graphical view ]
    Pfami PF02219. MTHFR. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51730. SSF51730. 1 hit.
    TIGRFAMsi TIGR00676. fadh2. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MC58.
    2. "Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254."
      Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.
      Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: NZ98/254 / Serogroup B.

    Entry informationi

    Entry nameiMETF_NEIMB
    AccessioniPrimary (citable) accession number: Q9JZQ3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present in outer membrane vesicle formulations which are used as vaccines in human.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3