Q9JZQ3 (METF_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5,10-methylenetetrahydrofolate reductase
EC=1.5.1.20

Gene names

Name:	metF
Ordered Locus Names:	NMB0943

Organism

Neisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]

Taxonomic identifier

122586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup B

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.
Cofactor	FAD By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Miscellaneous	Present in outer membrane vesicle formulations which are used as vaccines in human.
Sequence similarities	Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	methylenetetrahydrofolate reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	5,10-methylenetetrahydrofolate reductase		PRO_0000320271

Sequences

	Sequence		Length	Mass (Da)
	Q9JZQ3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 32B22282A681CB1F	FASTA	292	33,056
	10 20 30 40 50 60 MNYAKEINAL NNSLSDLKGD INVSFEFFPP KNEQMETMLW DSIHRLQTLH PKFVSVTYGA 70 80 90 100 110 120 NSGERDRTHG IVKRIKQETG LEAAPHLTGI DASPDELRQI AKDYWDSGIR RIVALRGDEP 130 140 150 160 170 180 AGYEKKPFYA EDLVKLLRSV ADFDISVAAY PEVHPEAKSA QADLINLKRK IDAGANHVIT 190 200 210 220 230 240 QFFFDVERYL RFRDRCVMLG IDVEIVPGIL PVTNFKQLGK MAQVTNVKIP SWLSQMYEGL 250 260 270 280 290 DDDQGTRNLV AASIAIDMVK VLSREGVKDF HFYTLNRSEL TYAICHILGV RP

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58.

[2]

"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254." Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X. Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: NZ98/254 / Serogroup B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF41349.1.
PIR	D81140.
RefSeq	NP_273981.1. NC_003112.2.
3D structure databases
HSSP	HSSP built from PDB template 2FMO based on UniProtKB P0AEZ1.
ProteinModelPortal	Q9JZQ3.
SMR	Q9JZQ3. Positions 3-292.
ModBase	Search...
Protein-protein interaction databases
STRING	122586.NMB0943.
Proteomic databases
PRIDE	Q9JZQ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF41349; AAF41349; NMB0943.
GeneID	903063.
KEGG	nme:NMB0943.
PATRIC	20357367. VBINeiMen85645_1198.
Phylogenomic databases
eggNOG	COG0685.
HOGENOM	HOG000246232.
KO	K00297.
OMA	SITQMFF.
ProtClustDB	PRK09432.
Enzyme and pathway databases
BioCyc	NMEN122586:GHGG-943-MONOMER.
UniPathway	UPA00193.
Family and domain databases
InterPro	IPR003171. Mehydrof_redctse. IPR004620. MTHF_reductase_bac. [Graphical view]
Pfam	PF02219. MTHFR. 1 hit. [Graphical view]
TIGRFAMs	TIGR00676. fadh2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METF_NEIMB

Accession

Primary (citable) accession number: Q9JZQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program