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Q9JZQ3 (METF_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Last modified December 11, 2013. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5,10-methylenetetrahydrofolate reductase

EC=1.5.1.20
Gene names
Name:metF
Ordered Locus Names:NMB0943
OrganismNeisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]
Taxonomic identifier122586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length292 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactor

FAD By similarity.

Pathway

One-carbon metabolism; tetrahydrofolate interconversion.

Miscellaneous

Present in outer membrane vesicle formulations which are used as vaccines in human.

Sequence similarities

Belongs to the methylenetetrahydrofolate reductase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2922925,10-methylenetetrahydrofolate reductase
PRO_0000320271

Regions

Nucleotide binding26 – 316NAD By similarity
Nucleotide binding57 – 604FAD By similarity
Nucleotide binding57 – 582NAD By similarity
Nucleotide binding116 – 1183FAD By similarity
Nucleotide binding129 – 1302FAD By similarity
Nucleotide binding154 – 1574FAD By similarity
Nucleotide binding163 – 1708FAD By similarity

Sites

Active site261Proton donor/acceptor By similarity
Binding site861FAD By similarity
Binding site1181Substrate By similarity
Binding site1501FAD By similarity
Binding site1811Substrate By similarity
Binding site2171Substrate By similarity
Binding site2731Substrate By similarity
Binding site2771Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JZQ3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 32B22282A681CB1F

FASTA29233,056
        10         20         30         40         50         60 
MNYAKEINAL NNSLSDLKGD INVSFEFFPP KNEQMETMLW DSIHRLQTLH PKFVSVTYGA 

        70         80         90        100        110        120 
NSGERDRTHG IVKRIKQETG LEAAPHLTGI DASPDELRQI AKDYWDSGIR RIVALRGDEP 

       130        140        150        160        170        180 
AGYEKKPFYA EDLVKLLRSV ADFDISVAAY PEVHPEAKSA QADLINLKRK IDAGANHVIT 

       190        200        210        220        230        240 
QFFFDVERYL RFRDRCVMLG IDVEIVPGIL PVTNFKQLGK MAQVTNVKIP SWLSQMYEGL 

       250        260        270        280        290 
DDDQGTRNLV AASIAIDMVK VLSREGVKDF HFYTLNRSEL TYAICHILGV RP 

« Hide

References

[1]"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58."
Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. expand/collapse author list , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C.
Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MC58.
[2]"Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254."
Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.
Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: NZ98/254 / Serogroup B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE002098 Genomic DNA. Translation: AAF41349.1.
PIRD81140.
RefSeqNP_273981.1. NC_003112.2.

3D structure databases

ProteinModelPortalQ9JZQ3.
SMRQ9JZQ3. Positions 3-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING122586.NMB0943.

Proteomic databases

PRIDEQ9JZQ3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF41349; AAF41349; NMB0943.
GeneID903063.
KEGGnme:NMB0943.
PATRIC20357367. VBINeiMen85645_1198.

Phylogenomic databases

eggNOGCOG0685.
HOGENOMHOG000246232.
KOK00297.
OMAFIRAETG.
OrthoDBEOG6D2KVW.
ProtClustDBPRK09432.

Enzyme and pathway databases

BioCycNMEN122586:GHGG-980-MONOMER.
UniPathwayUPA00193.

Family and domain databases

InterProIPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamPF02219. MTHFR. 1 hit.
[Graphical view]
TIGRFAMsTIGR00676. fadh2. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMETF_NEIMB
AccessionPrimary (citable) accession number: Q9JZQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: December 11, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways