SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9JZQ3

- METF_NEIMB

UniProt

Q9JZQ3 - METF_NEIMB

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
5,10-methylenetetrahydrofolate reductase
Gene
metF, NMB0943
Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-methyltetrahydrofolate + NAD(P)+ = 5,10-methylenetetrahydrofolate + NAD(P)H.

Cofactori

FAD By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei26 – 261Proton donor/acceptor By similarity
Binding sitei86 – 861FAD By similarity
Binding sitei118 – 1181Substrate By similarity
Binding sitei150 – 1501FAD By similarity
Binding sitei181 – 1811Substrate By similarity
Binding sitei217 – 2171Substrate By similarity
Binding sitei273 – 2731Substrate By similarity
Binding sitei277 – 2771Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 316NAD By similarity
Nucleotide bindingi57 – 604FAD By similarity
Nucleotide bindingi57 – 582NAD By similarity
Nucleotide bindingi116 – 1183FAD By similarity
Nucleotide bindingi129 – 1302FAD By similarity
Nucleotide bindingi154 – 1574FAD By similarity
Nucleotide bindingi163 – 1708FAD By similarity

GO - Molecular functioni

  1. methylenetetrahydrofolate reductase (NAD(P)H) activity Source: UniProtKB-EC

GO - Biological processi

  1. methionine biosynthetic process Source: UniProtKB-KW
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

FAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciNMEN122586:GHGG-980-MONOMER.
UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
5,10-methylenetetrahydrofolate reductase (EC:1.5.1.20)
Gene namesi
Name:metF
Ordered Locus Names:NMB0943
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000425: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2922925,10-methylenetetrahydrofolate reductase
PRO_0000320271Add
BLAST

Proteomic databases

PRIDEiQ9JZQ3.

Interactioni

Protein-protein interaction databases

STRINGi122586.NMB0943.

Structurei

3D structure databases

ProteinModelPortaliQ9JZQ3.
SMRiQ9JZQ3. Positions 3-292.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0685.
HOGENOMiHOG000246232.
KOiK00297.
OMAiLAMTHIL.
OrthoDBiEOG6D2KVW.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiIPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view]
PfamiPF02219. MTHFR. 1 hit.
[Graphical view]
SUPFAMiSSF51730. SSF51730. 1 hit.
TIGRFAMsiTIGR00676. fadh2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JZQ3-1 [UniParc]FASTAAdd to Basket

« Hide

MNYAKEINAL NNSLSDLKGD INVSFEFFPP KNEQMETMLW DSIHRLQTLH    50
PKFVSVTYGA NSGERDRTHG IVKRIKQETG LEAAPHLTGI DASPDELRQI 100
AKDYWDSGIR RIVALRGDEP AGYEKKPFYA EDLVKLLRSV ADFDISVAAY 150
PEVHPEAKSA QADLINLKRK IDAGANHVIT QFFFDVERYL RFRDRCVMLG 200
IDVEIVPGIL PVTNFKQLGK MAQVTNVKIP SWLSQMYEGL DDDQGTRNLV 250
AASIAIDMVK VLSREGVKDF HFYTLNRSEL TYAICHILGV RP 292
Length:292
Mass (Da):33,056
Last modified:October 1, 2000 - v1
Checksum:i32B22282A681CB1F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002098 Genomic DNA. Translation: AAF41349.1.
PIRiD81140.
RefSeqiNP_273981.1. NC_003112.2.

Genome annotation databases

EnsemblBacteriaiAAF41349; AAF41349; NMB0943.
GeneIDi903063.
KEGGinme:NMB0943.
PATRICi20357367. VBINeiMen85645_1198.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE002098 Genomic DNA. Translation: AAF41349.1 .
PIRi D81140.
RefSeqi NP_273981.1. NC_003112.2.

3D structure databases

ProteinModelPortali Q9JZQ3.
SMRi Q9JZQ3. Positions 3-292.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 122586.NMB0943.

Proteomic databases

PRIDEi Q9JZQ3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF41349 ; AAF41349 ; NMB0943 .
GeneIDi 903063.
KEGGi nme:NMB0943.
PATRICi 20357367. VBINeiMen85645_1198.

Phylogenomic databases

eggNOGi COG0685.
HOGENOMi HOG000246232.
KOi K00297.
OMAi LAMTHIL.
OrthoDBi EOG6D2KVW.

Enzyme and pathway databases

UniPathwayi UPA00193 .
BioCyci NMEN122586:GHGG-980-MONOMER.

Family and domain databases

Gene3Di 3.20.20.220. 1 hit.
InterProi IPR029041. FAD-linked_oxidoreductase-like.
IPR003171. Mehydrof_redctse.
IPR004620. MTHF_reductase_bac.
[Graphical view ]
Pfami PF02219. MTHFR. 1 hit.
[Graphical view ]
SUPFAMi SSF51730. SSF51730. 1 hit.
TIGRFAMsi TIGR00676. fadh2. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC58.
  2. "Proteomic analysis of a meningococcal outer membrane vesicle vaccine prepared from the group B strain NZ98/254."
    Vipond C., Suker J., Jones C., Tang C., Feavers I.M., Wheeler J.X.
    Proteomics 6:3400-3413(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: NZ98/254 / Serogroup B.

Entry informationi

Entry nameiMETF_NEIMB
AccessioniPrimary (citable) accession number: Q9JZQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Present in outer membrane vesicle formulations which are used as vaccines in human.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi