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Protein

Glutathione synthetase

Gene

gshB

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gshB)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi285Magnesium or manganeseUniRule annotation1
Metal bindingi287Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi155 – 211ATPUniRule annotationAdd BLAST57

GO - Molecular functioni

Keywordsi

Molecular functionLigase
Biological processGlutathione biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNMEN122586:G1G1B-1507-MONOMER
UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Alternative name(s):
GSH synthetaseUniRule annotation
Short name:
GSH-SUniRule annotation
Short name:
GSHaseUniRule annotation
Glutathione synthaseUniRule annotation
Gene namesi
Name:gshBUniRule annotation
Ordered Locus Names:NMB1559
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001974701 – 319Glutathione synthetaseAdd BLAST319

Proteomic databases

PaxDbiQ9JYJ3
PRIDEiQ9JYJ3

Interactioni

Protein-protein interaction databases

STRINGi122586.NMB1559

Structurei

3D structure databases

ProteinModelPortaliQ9JYJ3
SMRiQ9JYJ3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini129 – 314ATP-graspUniRule annotationAdd BLAST186

Sequence similaritiesi

Belongs to the prokaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105D7Z Bacteria
COG0189 LUCA
HOGENOMiHOG000265022
KOiK01920
OMAiWMRKDPP
OrthoDBiPOG091H04J6

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00162 GSH_S, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR006284 Glut_synth_pro
IPR004218 GSHS_ATP-bd
IPR004215 GSHS_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR21621:SF4 PTHR21621:SF4, 1 hit
PfamiView protein in Pfam
PF02955 GSH-S_ATP, 1 hit
PF02951 GSH-S_N, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01380 glut_syn, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JYJ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKVLFIADP MASFKTYKDT TYAMMREMAK RGWRLFHTLS GELSVNGGLV
60 70 80 90 100
TAQASAFEFL GAKNDDDHAW FKSADKVQTA LEAFDAVIMR TDPPFDMQYL
110 120 130 140 150
YATQLLTLAE QQGAKVFNSG QAMRDFNEKL AILNFSRFIA PTLVTTRSAD
160 170 180 190 200
VRTFLKEHGD IIIKPLDGMG GMGIFRLTEK DPNIGSILET LMQLDSRTIM
210 220 230 240 250
AQRYIPEIVH GDKRILIIGG EVVPYALARI PQNGETRGNL AAGGRGVAQE
260 270 280 290 300
LGGRDREIAE TLAPELKRRG ILLAGLDVIG SNLTEVNVTS PTGFQEIMKQ
310
KGFDVAAMFA DAVAAWSVR
Length:319
Mass (Da):35,146
Last modified:October 1, 2000 - v1
Checksum:i327C0F342F09C2F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA Translation: AAF41913.1
PIRiD81069
RefSeqiNP_274566.1, NC_003112.2
WP_002225038.1, NC_003112.2

Genome annotation databases

EnsemblBacteriaiAAF41913; AAF41913; NMB1559
GeneIDi904122
KEGGinme:NMB1559
PATRICifig|122586.8.peg.2006

Entry informationi

Entry nameiGSHB_NEIMB
AccessioniPrimary (citable) accession number: Q9JYJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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