Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9JYJ3 (GSHB_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:NMB1559
OrganismNeisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]
Taxonomic identifier122586 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197470

Regions

Domain129 – 314186ATP-grasp
Nucleotide binding155 – 21157ATP By similarity

Sites

Metal binding2851Magnesium or manganese By similarity
Metal binding2871Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JYJ3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 327C0F342F09C2F0

FASTA31935,146
        10         20         30         40         50         60 
MMKVLFIADP MASFKTYKDT TYAMMREMAK RGWRLFHTLS GELSVNGGLV TAQASAFEFL 

        70         80         90        100        110        120 
GAKNDDDHAW FKSADKVQTA LEAFDAVIMR TDPPFDMQYL YATQLLTLAE QQGAKVFNSG 

       130        140        150        160        170        180 
QAMRDFNEKL AILNFSRFIA PTLVTTRSAD VRTFLKEHGD IIIKPLDGMG GMGIFRLTEK 

       190        200        210        220        230        240 
DPNIGSILET LMQLDSRTIM AQRYIPEIVH GDKRILIIGG EVVPYALARI PQNGETRGNL 

       250        260        270        280        290        300 
AAGGRGVAQE LGGRDREIAE TLAPELKRRG ILLAGLDVIG SNLTEVNVTS PTGFQEIMKQ 

       310 
KGFDVAAMFA DAVAAWSVR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE002098 Genomic DNA. Translation: AAF41913.1.
PIRD81069.
RefSeqNP_274566.1. NC_003112.2.

3D structure databases

ProteinModelPortalQ9JYJ3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING122586.NMB1559.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF41913; AAF41913; NMB1559.
GeneID904122.
KEGGnme:NMB1559.
PATRIC20358974. VBINeiMen85645_2006.

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.

Enzyme and pathway databases

BioCycNMEN122586:GHGG-1600-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_NEIMB
AccessionPrimary (citable) accession number: Q9JYJ3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways