Reviewed, UniProtKB/Swiss-Prot Q9JXF1 (BICOA_NEIMB)

Names and origin

Protein names

Recommended name:
    Bifunctional enzyme birA/coaX
Including the following 2 domains:
    1- Recommended name:
            Biotin--[acetyl-CoA-carboxylase] synthetase
              EC=6.3.4.15
        Alternative name(s):
            Biotin--protein ligase
    2- Recommended name:
            Type III pantothenate kinase
              EC=2.7.1.33
        Alternative name(s):
            Pantothenic acid kinase
            PanK-III

Gene names

Name:	birA/coaX
Ordered Locus Names:	NMB2075

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Activates biotin to form biotinyl-5'-adenylate and transfers the biotin moiety to biotin-accepting proteins By similarity. Catalyzes the phosphorylation of pantothenate (Pan), the first step in CoA biosynthesis By similarity.
Catalytic activity	ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]. HAMAP MF_01274 ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate. HAMAP MF_01274
Cofactor	Monovalent cations By similarity.
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5. HAMAP MF_01274
Subcellular location	Cytoplasm Potential.
Sequence similarities	In the N-terminal section; belongs to the biotin--protein ligase family. In the C-terminal section; belongs to the type III pantothenate kinase family.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Biotin Nucleotide-binding
Molecular function	Kinase Ligase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	coenzyme A biosynthetic process Inferred from electronic annotation. Source: HAMAP positive regulation of transcription Inferred from electronic annotation. Source: InterPro protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP biotin-[acetyl-CoA-carboxylase] ligase activity Inferred from electronic annotation. Source: EC pantothenate kinase activity Inferred from electronic annotation. Source: HAMAP transcription activator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Bifunctional enzyme birA/coaX HAMAP MF_01274		PRO_0000270884
Regions
	Nucleotide binding	344 – 351	8	ATP By similarity
	Region	1 – 329	329	Biotin--protein ligase HAMAP MF_01274
	Region	336 – 592	257	Type III pantothenate kinase HAMAP MF_01274
	Region	433 – 436	4	Substrate binding By similarity
Sites
	Active site	435	1	Proton acceptor Potential
	Binding site	426	1	Substrate By similarity
	Binding site	458	1	ATP Potential
	Binding site	508	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JXF1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 9E27421DA2B41DE3	FASTA	592	64,701
	10 20 30 40 50 60 MTVLKLSHWR VLAELADGLP QHVSQLARMA DMKPQQLNGF WQQMPAHIRG LLRQHDGYWR 70 80 90 100 110 120 LVRPLAVFDA EGLRELGERS GFQTALKHEC ASSNDEILEL ARIAPDKAHK TICVTHLQSK 130 140 150 160 170 180 GRGRQGRKWS HRLGECLMFS FGWVFDRPQY ELGSLSPVAA VACRRALSRL GLDVQIKWPN 190 200 210 220 230 240 DLVVGRDKLG GILIETVRTG GKTVAVVGIG INFVLPKEVE NAASVQSLFQ TASRRGNADA 250 260 270 280 290 300 AVLLETLLVE LDAVLLQYAR DGFAPFVAEY QAANRDHGKA VLLLRDGETV FEGTVKGVDG 310 320 330 340 350 360 QGVLHLETAE GKQTVVSGEI SLRSDDRPVS VPKRRDSERF LLLDGGNSRL KWAWVENGTF 370 380 390 400 410 420 ATVGSAPYRD LSPLGAEWAE KADGNVRIVG CAVCGEFKKA QVQEQLARKI EWLPSSAQAL 430 440 450 460 470 480 GIRNHYRHPE EHGSDRWFNA LGSRRFSRNA CVVVSCGTAV TVDALTDDGH YLGGTIMPGF 490 500 510 520 530 540 HLMKESLAVR TANLNRHAGK RYPFPTTTGN AVASGMMDAV CGSVMMMHGR LKEKTGAGKP 550 560 570 580 590 VDVIITGGGA AKVAEALPPA FLAENTVRVA DNLVIYGLLN MIAAEGREYE HI

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002098 Genomic DNA. Translation: AAF42394.1.
PIR	B81009.
RefSeq	NP_275065.1.
3D structure databases
HSSP	HSSP built from PDB template 1BIA based on UniProtKB P06709.
ModBase	Search...
Genome annotation databases
GeneID	903989.
GenomeReviews	Gene locus NMB2075 in contig AE002098_GR.
KEGG	nme:NMB2075.
NMPDR	fig|122586.1.peg.1999.
TIGR	NMB2075.
Phylogenomic databases
HOGENOM	Q9JXF1.
OMA	HECASSN.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_2075-MON.
BRENDA	2.7.1.33. 293828. 6.3.4.15. 293828.
Family and domain databases
HAMAP	MF_01274. Fused. [Tree]
InterPro	IPR004619. Baf. IPR004408. Biotin_CoA_COase_ligase. IPR003142. BPL_C. IPR004143. BPL_LipA_LipB. [Graphical view]
PANTHER	PTHR12835. BirA_ligase. 1 hit.
Pfam	PF02237. BPL_C. 1 hit. PF03099. BPL_LipA_LipB. 1 hit. PF03309. Bvg_acc_factor. 1 hit. [Graphical view]
TIGRFAMs	TIGR00671. baf. 1 hit. TIGR00121. birA_ligase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BICOA_NEIMB

Accession

Primary (citable) accession number: Q9JXF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)