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Protein

Argininosuccinate synthase

Gene

argG

Organism
Neisseria meningitidis serogroup B (strain MC58)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei46 – 461ATP; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei102 – 1021CitrullineBy similarity
Binding sitei132 – 1321ATP; via amide nitrogenBy similarity
Binding sitei134 – 1341AspartateBy similarity
Binding sitei134 – 1341ATPBy similarity
Binding sitei138 – 1381AspartateBy similarity
Binding sitei138 – 1381CitrullineBy similarity
Binding sitei139 – 1391AspartateBy similarity
Binding sitei139 – 1391ATPBy similarity
Binding sitei142 – 1421CitrullineBy similarity
Binding sitei195 – 1951CitrullineBy similarity
Binding sitei197 – 1971ATPBy similarity
Binding sitei204 – 2041CitrullineBy similarity
Binding sitei206 – 2061CitrullineBy similarity
Binding sitei283 – 2831CitrullineBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 289ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciNMEN122586:GHGG-2194-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthase (EC:6.3.4.5)
Alternative name(s):
Citrulline--aspartate ligase
Gene namesi
Name:argG
Ordered Locus Names:NMB2129
OrganismiNeisseria meningitidis serogroup B (strain MC58)
Taxonomic identifieri122586 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000425 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447Argininosuccinate synthasePRO_0000148702Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi122586.NMB2129.

Structurei

3D structure databases

ProteinModelPortaliQ9JXC1.
SMRiQ9JXC1. Positions 6-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JXC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQNHTILQS LPVGQKVGIA FSGGLDTSAA LLWMKLKGAL PYAYTANLGQ
60 70 80 90 100
PDEDDYNAIP KKAMEYGAEN ARLIDCRAQL AHEGIAAIQC GAFHVSTGGI
110 120 130 140 150
AYFNTTPLGR AVTGTMLVSA MKEDDVNIWG DGSTYKGNDI ERFYRYGLLT
160 170 180 190 200
NPALKIYKPW LDQQFIDELG GRHEMSEFLI ANGFNYKMSV EKAYSTDSNM
210 220 230 240 250
LGATHEAKDL EFLNSGIKIV KPIMGVAFWD ENVEVSPEEV SVRFEEGVPV
260 270 280 290 300
ALNGKEYADP VELFLEANRI GGRHGLGMSD QIENRIIEAK SRGIYEAPGM
310 320 330 340 350
ALFHIAYERL VTGIHNEDTI EQYRINGLRL GRLLYQGRWF DSQALMLRET
360 370 380 390 400
AQRWVAKAVT GEVTLELRRG NDYSILNTES PNLTYQPERL SMEKVEDAAF
410 420 430 440
TPLDRIGQLT MRNLDITDTR VKLGIYSQSG LLSLGEGSVL PQLGNKQ
Length:447
Mass (Da):49,664
Last modified:October 1, 2000 - v1
Checksum:i8A11C502DB4C0506
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA. Translation: AAF42437.1.
PIRiB81003.
RefSeqiNP_275114.1. NC_003112.2.
WP_002223213.1. NC_003112.2.

Genome annotation databases

EnsemblBacteriaiAAF42437; AAF42437; NMB2129.
GeneIDi903396.
KEGGinme:NMB2129.
PATRICi20360434. VBINeiMen85645_2713.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE002098 Genomic DNA. Translation: AAF42437.1.
PIRiB81003.
RefSeqiNP_275114.1. NC_003112.2.
WP_002223213.1. NC_003112.2.

3D structure databases

ProteinModelPortaliQ9JXC1.
SMRiQ9JXC1. Positions 6-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi122586.NMB2129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF42437; AAF42437; NMB2129.
GeneIDi903396.
KEGGinme:NMB2129.
PATRICi20360434. VBINeiMen85645_2713.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230094.
KOiK01940.
OMAiMRNLDIA.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciNMEN122586:GHGG-2194-MONOMER.

Family and domain databases

Gene3Di1.10.287.400. 1 hit.
3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00581. Arg_succ_synth_type2.
InterProiIPR023437. Arg_succ_synth_type2_subfam.
IPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR024074. AS_cat/multimer_dom_body.
IPR024073. AS_multimer_C_tail.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MC58.

Entry informationi

Entry nameiASSY_NEIMB
AccessioniPrimary (citable) accession number: Q9JXC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.