Q9JXA3 (PUR2_NEIMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	NMB2151

Organism

Neisseria meningitidis serogroup B (strain MC58) [Reference proteome] [HAMAP]

Taxonomic identifier

122586 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup B

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138		PRO_0000151467
Regions
	Domain	107 – 314	208	ATP-grasp
	Nucleotide binding	133 – 194	62	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JXA3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7203DDCAEC2D38E2	FASTA	423	44,975
	10 20 30 40 50 60 MKLLVIGNGG REHALAWKLA QSPKVETVFV APGNAGTAIE PKLQNIDLTA HQDLIEFCRK 70 80 90 100 110 120 ENIVFTVVGP EAPLAAGIVD DFRAAGLKIF GPTQYAAQLE SSKDFAKAFM AKYNIPTAQY 130 140 150 160 170 180 QTFENADAAH DYVNQKGAPI VIKADGLAAG KGVIVAMTLD EAHAAIDDML LDNKMGNAGA 190 200 210 220 230 240 RVVIEDFLQG EEASFIVMVD GNNVLPMATS QDHKRLLDGD KGLNTGGMGA YSPAPVVTPV 250 260 270 280 290 300 VYERAMNEII LPTVAGMKAE GHEFTGFLYA GLMIDQSGAP YTIEFNCRFG DPETQPIMSR 310 320 330 340 350 360 LNSDLSDLVE AAIDGKLDSV TAEWSPQTAV GVVLAAQNYP ETPKKGDIIS GLDAANQIGK 370 380 390 400 410 420 VFHAGTTANE KGDVLTNGGR VLCVVGLGDN VAQAKAKAYG ALEKISFDGM QYRKDIADKA INR

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE002098 Genomic DNA. Translation: AAF42459.1.
PIR	E81000.
RefSeq	NP_275136.1. NC_003112.2.
3D structure databases
ProteinModelPortal	Q9JXA3.
SMR	Q9JXA3. Positions 1-423.
ModBase	Search...
Protein-protein interaction databases
STRING	122586.NMB2151.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF42459; AAF42459; NMB2151.
GeneID	903221.
KEGG	nme:NMB2151.
PATRIC	20360498. VBINeiMen85645_2745.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033463.
KO	K01945.
OMA	MGAYTPL.
ProtClustDB	PRK00885.
Enzyme and pathway databases
BioCyc	NMEN122586:GHGG-2146-MONOMER.
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
HAMAP	MF_00138. GARS.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_NEIMB

Accession

Primary (citable) accession number: Q9JXA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program