Reviewed, UniProtKB/Swiss-Prot Q9JXA3 (PUR2_NEIMB)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	NMB2151

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151467
Regions
	Domain	107 – 314	208	ATP-grasp
	Nucleotide binding	133 – 194	62	ATP By similarity
Sites
	Metal binding	284	1	Magnesium or manganese By similarity
	Metal binding	286	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JXA3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7203DDCAEC2D38E2	FASTA	423	44,975
	10 20 30 40 50 60 MKLLVIGNGG REHALAWKLA QSPKVETVFV APGNAGTAIE PKLQNIDLTA HQDLIEFCRK 70 80 90 100 110 120 ENIVFTVVGP EAPLAAGIVD DFRAAGLKIF GPTQYAAQLE SSKDFAKAFM AKYNIPTAQY 130 140 150 160 170 180 QTFENADAAH DYVNQKGAPI VIKADGLAAG KGVIVAMTLD EAHAAIDDML LDNKMGNAGA 190 200 210 220 230 240 RVVIEDFLQG EEASFIVMVD GNNVLPMATS QDHKRLLDGD KGLNTGGMGA YSPAPVVTPV 250 260 270 280 290 300 VYERAMNEII LPTVAGMKAE GHEFTGFLYA GLMIDQSGAP YTIEFNCRFG DPETQPIMSR 310 320 330 340 350 360 LNSDLSDLVE AAIDGKLDSV TAEWSPQTAV GVVLAAQNYP ETPKKGDIIS GLDAANQIGK 370 380 390 400 410 420 VFHAGTTANE KGDVLTNGGR VLCVVGLGDN VAQAKAKAYG ALEKISFDGM QYRKDIADKA INR

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002098 Genomic DNA. Translation: AAF42459.1.
PIR	E81000.
RefSeq	NP_275136.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	903221.
GenomeReviews	Gene locus NMB2151 in contig AE002098_GR.
KEGG	nme:NMB2151.
NMPDR	fig|122586.1.peg.2070.
TIGR	NMB2151.
Phylogenomic databases
HOGENOM	Q9JXA3.
OMA	IEYNCRF.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_2151-MON.
BRENDA	6.3.4.13. 293828.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_NEIMB

Accession

Primary (citable) accession number: Q9JXA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)