Q9JWJ3 (SYC_NEIMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Ordered Locus Names:	NMA0347

Organism

Neisseria meningitidis serogroup A / serotype 4A (strain Z2491) [Complete proteome] [HAMAP]

Taxonomic identifier

122587 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria › Neisseria meningitidis › Neisseria meningitidis serogroup A

Protein attributes

Sequence length	699 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP-Rule MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 699	699	Cysteine--tRNA ligase HAMAP-Rule MF_00041		PRO_0000159443
Regions
	Region	1 – 226	226	Unknown HAMAP-Rule MF_00041
	Motif	256 – 266	11	"HIGH" region HAMAP-Rule MF_00041
	Motif	508 – 512	5	"KMSKS" region HAMAP-Rule MF_00041
Sites
	Metal binding	254	1	Zinc By similarity
	Metal binding	435	1	Zinc By similarity
	Metal binding	460	1	Zinc By similarity
	Metal binding	464	1	Zinc By similarity
	Binding site	511	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JWJ3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6C69AEDB9103ABEB	FASTA	699	78,973
	10 20 30 40 50 60 MTTITEKRLT FAFPEDYYVT KYDEWEHYKI FQNSCNLRNK IDTNEKGKNG INQSVDDDNG 70 80 90 100 110 120 SSGVDIIALH ESTLWLIEIK DYYRLGLEPN AQSIDEKLSD LPYLIARKIR DSLAGLVSAK 130 140 150 160 170 180 FKAEKQEEKD FSRLALDCNE IKIVLHIEMP SIRSKLYPSS SDLANLLKDK FKLSEFTKNF 190 200 210 220 230 240 ANCYAEPIFT NISHINNPQL RNVPWSVSTG TEQKLSSEQQ RLIHNPMTTI YNTLTRQKEP 250 260 270 280 290 300 FAPIDPKNVR MYVCGMTVYD YCHLGHARVM VVFDMIARWL RECGYPLTYV RNITDIDDKI 310 320 330 340 350 360 IARAAENGET IGELTARFIQ AMHEDADALG VLRPDIEPKA TENIPQMIAM IETLIQNGKA 370 380 390 400 410 420 YPAANGDVYY AVREFSAYGQ LSGKSLDDLR AGERVEVDGF KRDPLDFVLW KAAKAGEPAW 430 440 450 460 470 480 ESPWGNGRPG WHIECSAMSE NLFGNTFDIH GGGADLQFPH HENEIAQSVG ATGHTCGHHH 490 500 510 520 530 540 AQTHHGQSIA SHVKYWLHNG FIRVDGEKMS KSLGNFFTIR EVLKQYDPEV VRFFILRAHY 550 560 570 580 590 600 RSPLNYSDAH LDDAKGALTR LYTTLKNTPA AEFDLSENVN DYTRRFYAAM NDDFGTVEAV 610 620 630 640 650 660 AVLFELAGEV NKTNDAQLAG CLKALGGIIG LLQRDPTEFL QGGAASDGLS NEEIEDLIAR 670 680 690 RKQARADKNW AESDRIRDLL NEHKIILEDN AGGTTWRRG

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL157959 Genomic DNA. Translation: CAM07647.1.
PIR	H82030.
RefSeq	YP_002341860.1. NC_003116.1.
3D structure databases
ProteinModelPortal	Q9JWJ3.
SMR	Q9JWJ3. Positions 227-640.
ModBase	Search...
Protein-protein interaction databases
STRING	122587.NMA0347.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAM07647; CAM07647; NMA0347.
GeneID	906353.
KEGG	nma:NMA0347.
PATRIC	20361360. VBINeiMen132687_0405.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0215.
HOGENOM	HOG000245250.
KO	K01883.
OMA	DFDALNM.
ProtClustDB	PRK14535.
Enzyme and pathway databases
BioCyc	NMEN122587:GI3Q-348-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
HAMAP	MF_00041. Cys_tRNA_synth. Divergent sequence.
InterPro	IPR015803. Cys-tRNA-ligase. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
PANTHER	PTHR10890. PTHR10890. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_NEIMA

Accession

Primary (citable) accession number: Q9JWJ3
Secondary accession number(s): A1IPH8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program