Reviewed, UniProtKB/Swiss-Prot Q9JWC5 (PUR4_NEIMA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine synthase
      Short name=FGAM synthase
      Short name=FGAMS
    EC=6.3.5.3
Alternative name(s):
    Formylglycinamide ribotide amidotransferase
      Short name=FGARAT
    Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	NMA0445

Organism

Neisseria meningitidis serogroup A [Complete proteome] [HAMAP]

Taxonomic identifier

65699 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	1320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1320	1320	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100410
Regions
	Domain	1069 – 1320	252	Glutamine amidotransferase type-1
	Nucleotide binding	311 – 322	12	ATP Potential
Sites
	Active site	1162	1	Nucleophile By similarity
	Active site	1285	1	By similarity
	Active site	1287	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JWC5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FEB32DC315CEDD50	FASTA	1,320	143,791
	10 20 30 40 50 60 MSVVLPLRGV TALSDFRVEK LLQKAAALGL PEVKLSSEFW YFVGSEKALD AATVEKLQAL 70 80 90 100 110 120 LAAQSVEQTP KAREGLHLFL VTPRLGTISP WASKATNIAE NCGLAGIERI ERGMAVWLEG 130 140 150 160 170 180 ALTDEQQQQW AALLHDRMTE SVLPDFQTAS KLFHHLESET FSTVDVLGGG KEALVKANTE 190 200 210 220 230 240 MGLALSADEI DYLVENYQAL QRNPSDVELM MFAQANSEHC RHKIFNADFI LNGEKQPKSL 250 260 270 280 290 300 FGMIRDTHNA HPEGTVVAYK DNSSVIEGAK VERFYPNAAE NQGYRFHEED THIIMKVETH 310 320 330 340 350 360 NHPTAIAPFA GAATGAGGEI RDEGATGKGS RPKAGLTGFT VSNLNIPGLK QPWEQDYGKP 370 380 390 400 410 420 EHISSPLDIM IEGPIGGAAF NNEFGRPNLL GYFRTFEEKF DGQVRGYHKP IMIAGGLGSI 430 440 450 460 470 480 QAQQTHKDEI PEGALLIQLG GPGMLIGLGG GAASSMDTGT NDASLDFNSV QRGNPEIERR 490 500 510 520 530 540 AQEVIDRCWQ LGDKNPIISI HDVGAGGLSN AFPELVNDAG RGAVFKLREV PLEEHGLNPL 550 560 570 580 590 600 QIWCNESQER YVLSILEKDL DIFRSICERE RCPFAVVGTA TDDGHLKVRD DLFSNNPVDL 610 620 630 640 650 660 PLNVLLGKPP KTTRTDKTVA PSKKPFHAGD IDITEAAYRV LRLPAVAAKN FLITIGDRSV 670 680 690 700 710 720 GGMTHRDQMV GKYQTPVADC AVTMMGFNTY RGEAMSMGEK PTVALFDAPA SGRMCVGEAI 730 740 750 760 770 780 TNIAAANIGD IGNIKLSANW MAACGNEGED EKLYRTVEAV SKACQALDLS IPVGKDSLSM 790 800 810 820 830 840 KTVWQDGEEK KSVVSPLSLI ISAFAPVQDV RKTVTPELKN VEDSVLLFVD LGFGKARMGG 850 860 870 880 890 900 SAFGQVYNNM SGDAPDLDDT SRLKAFYNVI QQLVAEDKLL AYHDRSDGGL FAVLVEMAFA 910 920 930 940 950 960 GRCGLDIDLN LLLAQTFITN HTALSQSLRT EEVKALAEWQ ETIARTLFNE ELGAVIQVRK 970 980 990 1000 1010 1020 QDVADIINLF YQQQLHHNVF EIGTLTDENT LIIRDGQTHL ISDNLIKLQQ TWQETSHQIQ 1030 1040 1050 1060 1070 1080 RLRDNPACAD SEFALIGDNG RSALFANLKF DVNEDIAAPF INSGAKPKIA ILREQGVNGQ 1090 1100 1110 1120 1130 1140 IEMAAAFTRA GFDAYDVHMS DLMAGRVHLA DFKMLAACGG FSYGDVLGAG KGWAKSILFH 1150 1160 1170 1180 1190 1200 PALRDQFAAF FTDPNTLTLG VCNGCQMVSN LAEIIPGTAG WPKFKRNLSE QFEARLSMVH 1210 1220 1230 1240 1250 1260 VPKSASLILN EMQGSSLPVV VSHGEGRADF ALHGGNISAD LGIALQYVDG QNQITQTYPL 1270 1280 1290 1300 1310 1320 NPNGSPQGIA GVTNADGRVT IMMPHPERVY RAAQMSWKPE DWTELSGWYR LFAGARKALG

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed: 10761919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491 / Serogroup A / Serotype 4A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL157959 Genomic DNA. Translation: CAM07730.1.
PIR	G81961.
RefSeq	YP_002341941.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	906445.
GenomeReviews	Gene locus NMA0445 in contig AL157959_GR.
KEGG	nma:NMA0445.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9JWC5.
OMA	ERGIAYY.
Enzyme and pathway databases
BioCyc	NMEN122587:NMA0445-MON.
BRENDA	6.3.5.3. 292829.
Family and domain databases
HAMAP	MF_00419. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_NEIMA

Accession

Primary (citable) accession number: Q9JWC5
Secondary accession number(s): A1IPQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	October 1, 2000
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)