Reviewed, UniProtKB/Swiss-Prot Q9JVZ6 (CARA_NEIMA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	NMA0608

Organism

Neisseria meningitidis serogroup A [Complete proteome] [HAMAP]

Taxonomic identifier

65699 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112298
Regions
	Domain	190 – 377	188	Glutamine amidotransferase type-1
	Region	1 – 186	186	CPSase HAMAP MF_01209
Sites
	Active site	266	1	Nucleophile By similarity
	Active site	350	1	By similarity
	Active site	352	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JVZ6-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1C64175800E26272	FASTA	377	40,605
	10 20 30 40 50 60 MSTPALLVLA DGSVFHGTSI GYEGSTSGEV VFNTSMTGYQ EILTDPSYCK QIVTLTYPHI 70 80 90 100 110 120 GNTGTNAEDE ESRSVYAAGL IIRDLPLLHS NFRASESLHD YLVRNKTVAI ADIDTRRLTT 130 140 150 160 170 180 LLREKGAQGG AILTGADATI EKAQELIAAF GSMVGKDLAK EVSCTETYEW TEGEWALGKG 190 200 210 220 230 240 FVTPDEQPYH VVAYDFGVKT NILRMLASRG CRLTVVPAQT SAQDVLALNP DGVFLSNGPG 250 260 270 280 290 300 DPEPCTYAIE AVQKLMESGK PIFGICLGHQ LISLAIGAKT LKMRFSHHGA NHPVQDLDSG 310 320 330 340 350 360 KVVITSQNHG FAVDADTLPA NARITHKSLF DNTLQGIELT DKPVFCFQGH PEASPGPQDV 370 GYLFDKFIGN MKAAKRA

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed: 10761919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491 / Serogroup A / Serotype 4A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL157959 Genomic DNA. Translation: CAM07875.1.
PIR	D81980.
RefSeq	YP_002342081.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	906603.
GenomeReviews	Gene locus NMA0608 in contig AL157959_GR.
KEGG	nma:NMA0608.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9JVZ6.
OMA	Q9JVZ6. LFDGSNC.
Enzyme and pathway databases
BioCyc	NMEN122587:NMA0608-MON.
BRENDA	6.3.5.5. 292829.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_NEIMA

Accession

Primary (citable) accession number: Q9JVZ6
Secondary accession number(s): A1IQ49

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)