Reviewed, UniProtKB/Swiss-Prot Q9JUV5 (LST_NEIMA)

Names and origin

Protein names

Recommended name:
    CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase
      Short name=Beta-galactoside alpha-2,3-sialyltransferase
      Short name=Alpha 2,3-ST
    EC=2.4.99.-
Alternative name(s):
    Lipooligosaccharide sialyltransferase

Gene names

Name:	lst
Ordered Locus Names:	NMA1118

Organism

Neisseria meningitidis serogroup A [Complete proteome] [HAMAP]

Taxonomic identifier

65699 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transfers sialic acid from the substrate CMP-sialic acid donor to the terminal beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine on the lacto-N-neotetraose branch of the lipooligosaccharide.
Catalytic activity	CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-acetyl-beta-D-glucosamine = CMP + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-N-acetyl-beta-D-glucosamine.
Pathway	Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Sequence similarities	Belongs to the glycosyltransferase 52 family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	sialyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase		PRO_0000080573

Sequences

	Sequence		Length	Mass (Da)
	Q9JUV5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F2D3F60DC588AC2B	FASTA	371	42,554
	10 20 30 40 50 60 MGLKKACLTV LCLIVFCFGI FYTFDRVNQG ERNAVSLLKD KLFNEEGEPV NLIFCYTILQ 70 80 90 100 110 120 MKVAERIMAQ HPGERFYVVL MSENRNEKYD YYFNQIKDKA ERAYFFHLPY GLNKSFNFIP 130 140 150 160 170 180 TMAELKVKSM LLPKVKRIYL ASLEKVSIAA FLSTYPDAEI KTFDDGTGNL IQSSSYLGDE 190 200 210 220 230 240 FSVNGTIKRN FARMMIGDWS IAKTRNASDE HYTIFKGLKN IMDDGRRKMT YLPLFDASEL 250 260 270 280 290 300 KAGDETGGTV RILLGSPDKE MKEISEKAAK NFNIQYVAPH PRQTYGLSGV TTLNSPYVIE 310 320 330 340 350 360 DYILREIKKN PHTRYEIYTF FSGAALTMKD FPNVHVYALK PASLPEDYWL KPVYALFTQS 370 GIPILTFDDK D

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed: 10761919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491 / Serogroup A / Serotype 4A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL157959 Genomic DNA. Translation: CAM08326.1.
PIR	B81878.
RefSeq	YP_002342521.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT52. Glycosyltransferase Family 52.
Genome annotation databases
GeneID	907087.
GenomeReviews	Gene locus NMA1118 in contig AL157959_GR.
KEGG	nma:NMA1118.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9JUV5.
OMA	Q9JUV5. ERIMAQH.
Enzyme and pathway databases
BioCyc	NMEN122587:NMA1118-MON.
Family and domain databases
InterPro	IPR012477. Glyco_transf_52. [Graphical view]
Pfam	PF07922. Glyco_transf_52. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LST_NEIMA

Accession

Primary (citable) accession number: Q9JUV5
Secondary accession number(s): A1IRE0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)