Reviewed, UniProtKB/Swiss-Prot Q9JUD5 (CYSH_NEIMA)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	NMA1366

Organism

Neisseria meningitidis serogroup A [Complete proteome] [HAMAP]

Taxonomic identifier

65699 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100635

Sequences

	Sequence		Length	Mass (Da)
	Q9JUD5-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: F47196A2C19A0C52	FASTA	244	28,063
	10 20 30 40 50 60 MKIFKPALWK IPPIENGSET ALAEKTETLK QRLHRIAGSH RDARFASSLA AEDMVITDLI 70 80 90 100 110 120 AGENLNIGIF TLDTGLLHAE TLNLLDRIER VYPHMQIKRF QPIREDALHY VESKGRFAFY 130 140 150 160 170 180 DSVEARRECC RIRKTEPLDR AIAGADAWLT GQRREQSATR TELPFAEYDA GRGIDKYNPI 190 200 210 220 230 240 FDWSEHDVWA YILANNVPYN DLYRQGFPSI GCDPCTRPVK AGEDIRAGRW WWEDKNSKEC GLHK

References

[1]

"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491." Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G. Nature 404:502-506(2000) [PubMed: 10761919] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z2491 / Serogroup A / Serotype 4A.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL157959 Genomic DNA. Translation: CAM08539.1.
PIR	H81905.
RefSeq	YP_002342720.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	907773.
GenomeReviews	Gene locus NMA1366 in contig AL157959_GR.
KEGG	nma:NMA1366.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9JUD5.
OMA	Q9JUD5. WWWENPE.
Enzyme and pathway databases
BioCyc	NMEN122587:NMA1366-MON.
BRENDA	1.8.4.8. 292829.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_NEIMA

Accession

Primary (citable) accession number: Q9JUD5
Secondary accession number(s): A1IRZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)