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Q9JTJ6 (GSHB_NEIMA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:NMA1747
OrganismNeisseria meningitidis serogroup A / serotype 4A (strain Z2491) [Complete proteome] [HAMAP]
Taxonomic identifier122587 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Magnesium
Manganese
Metal-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytosol

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutathione synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197469

Regions

Domain128 – 313186ATP-grasp
Nucleotide binding154 – 21057ATP By similarity

Sites

Metal binding2841Magnesium or manganese By similarity
Metal binding2861Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JTJ6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: D22681F6229E7BD6

FASTA31835,172
        10         20         30         40         50         60 
MKVLFIADPM ASFKTYKDTT YAMMREMAKR GWRLFHTLSG ELSVKNGLVV ADASAFEFLG 

        70         80         90        100        110        120 
AQDDHDKNWF AAADKVQTAL KAFDAVIMRT DPPFDMQYLY ATQLLTLAEQ QGAKVFNSGQ 

       130        140        150        160        170        180 
AMRDFNEKLA ILNFSRFTAP TLVTTRSADV RAFLKEHGDI IIKPLDGMGG MGIFRLTEKD 

       190        200        210        220        230        240 
PNIGSILETL MQLDSRTIMA QRYIPEIVHG DKRILIIGGE VVPYALARIP QNGETRGNLA 

       250        260        270        280        290        300 
AGGRGVAQEL SERDREIAET LAPELKRRGI LLAGLDVIGS NLTEVNVTSP TGFQEIMKQK 

       310 
GFDVAAMFAD AVAEWSVR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL157959 Genomic DNA. Translation: CAM08875.1.
PIRC81799.
RefSeqYP_002343039.1. NC_003116.1.

3D structure databases

ProteinModelPortalQ9JTJ6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING122587.NMA1747.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM08875; CAM08875; NMA1747.
GeneID907674.
KEGGnma:NMA1747.
PATRIC20364784. VBINeiMen132687_2095.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAPTCFQEI.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycNMEN122587:GI3Q-1592-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_NEIMA
AccessionPrimary (citable) accession number: Q9JTJ6
Secondary accession number(s): A1ISW3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: February 19, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways