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Q9JTG4

- SYA_NEIMA

UniProt

Q9JTG4 - SYA_NEIMA

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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi562 – 5621ZincUniRule annotation
Metal bindingi566 – 5661ZincUniRule annotation
Metal bindingi664 – 6641ZincUniRule annotation
Metal bindingi668 – 6681ZincUniRule annotation

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciNMEN122587:GI3Q-1636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:NMA1788
OrganismiNeisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Taxonomic identifieri122587 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000626: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Alanine--tRNA ligasePRO_0000075160Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi122587.NMA1788.

Structurei

3D structure databases

ProteinModelPortaliQ9JTG4.
SMRiQ9JTG4. Positions 1-460.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156964.
KOiK01872.
OMAiKYHTATH.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JTG4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKTSELRQKF LKFFETKGHT VVRSSSLVPH DDPTLLFTNA GMNQFKDVFL
60 70 80 90 100
GFDKRPYSRA TTAQKCVRAG GKHNDLENVG YTARHHTFFE MMGNFSFGDY
110 120 130 140 150
FKRDAIHFAW EFLTSPEWLN IPKDKLLATV YAEDDEAYNI WLNEIGMPSE
160 170 180 190 200
RIVRIGDNKG AKYVSDNFWQ MGDTGPCGPC SEIFYDHGEE IWGGIPGSPE
210 220 230 240 250
EDGDRWIEIW NCVFMQFNRD EQGNMNPLPK PSVDTGMGLE RMAAVMQHVH
260 270 280 290 300
SNYEIDLFQD LLKAVARETG AAFSMDEPSL KVIADHIRSC SFLIADGVLP
310 320 330 340 350
SNEGRGYVLR RIIRRAVRHG YKLGQSKPFF HKLVADLVKE MGGAYPELKE
360 370 380 390 400
KQAQIEEALK NEESRFAQTL ETGMALLENA LAKGGKTLDG EIIFKLYDTY
410 420 430 440 450
GFPYDLTADI CRERNIELDE AGFEREMEAQ RARARAAQSF KANAQLPYDG
460 470 480 490 500
QDTEFKGYSE RQTESKVLAL YKDGEQVNEL NEGDSGAVVI DFTPFYAESG
510 520 530 540 550
GQVGDVGYIF SSENRFEVRD TQKIKAAVFG QFGVQTSGRL KVGDSVTAKV
560 570 580 590 600
DDEIRNANMR NHSATHLMHK ALRDVLGGHV EQKGSLVTAE STRFDISHPQ
610 620 630 640 650
AVTAEEIAEV ERRVNEAVLA NVAVNAAIMS MEDAQKTGAM MLFGEKYGEE
660 670 680 690 700
VRVLQMGGFS TELCGGTHVS RTGDIGLFKI ISEGGIAAGV RRIEAITGLN
710 720 730 740 750
ALKWAQEQER LVKDIIAETK AQTEKDVLAK IQAGAAHAKA LEKELARAKA
760 770 780 790 800
ELAVHAGAKL LDDAKDLGAA KLVAAQIEAD AAALREIVTD LTGKSDNAVI
810 820 830 840 850
LLAAVNDGKV SLCAGVSKPL TGKVKAGDLV KFAAEQVGGK GGGRPDLAQA
860 870
GGTDAGKLPE MLVSVESWLC QKLS
Length:874
Mass (Da):96,032
Last modified:October 1, 2000 - v1
Checksum:i17EE6A14668211EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157959 Genomic DNA. Translation: CAM08912.1.
PIRiC81804.
RefSeqiYP_002343076.1. NC_003116.1.

Genome annotation databases

EnsemblBacteriaiCAM08912; CAM08912; NMA1788.
GeneIDi907637.
KEGGinma:NMA1788.
PATRICi20364887. VBINeiMen132687_2139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157959 Genomic DNA. Translation: CAM08912.1 .
PIRi C81804.
RefSeqi YP_002343076.1. NC_003116.1.

3D structure databases

ProteinModelPortali Q9JTG4.
SMRi Q9JTG4. Positions 1-460.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 122587.NMA1788.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM08912 ; CAM08912 ; NMA1788 .
GeneIDi 907637.
KEGGi nma:NMA1788.
PATRICi 20364887. VBINeiMen132687_2139.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156964.
KOi K01872.
OMAi KYHTATH.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci NMEN122587:GI3Q-1636-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z2491.

Entry informationi

Entry nameiSYA_NEIMA
AccessioniPrimary (citable) accession number: Q9JTG4
Secondary accession number(s): A1IT00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3