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Q9JTG4

- SYA_NEIMA

UniProt

Q9JTG4 - SYA_NEIMA

Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

    Catalytic activityi

    ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi562 – 5621ZincUniRule annotation
    Metal bindingi566 – 5661ZincUniRule annotation
    Metal bindingi664 – 6641ZincUniRule annotation
    Metal bindingi668 – 6681ZincUniRule annotation

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciNMEN122587:GI3Q-1636-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
    Alternative name(s):
    Alanyl-tRNA synthetaseUniRule annotation
    Short name:
    AlaRSUniRule annotation
    Gene namesi
    Name:alaSUniRule annotation
    Ordered Locus Names:NMA1788
    OrganismiNeisseria meningitidis serogroup A / serotype 4A (strain Z2491)
    Taxonomic identifieri122587 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
    ProteomesiUP000000626: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 874874Alanine--tRNA ligasePRO_0000075160Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi122587.NMA1788.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JTG4.
    SMRiQ9JTG4. Positions 1-460.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0013.
    HOGENOMiHOG000156964.
    KOiK01872.
    OMAiKYHTATH.
    OrthoDBiEOG6Q2SQ2.

    Family and domain databases

    HAMAPiMF_00036_B. Ala_tRNA_synth_B.
    InterProiIPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR00980. TRNASYNTHALA.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsiTIGR00344. alaS. 1 hit.
    PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JTG4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTSELRQKF LKFFETKGHT VVRSSSLVPH DDPTLLFTNA GMNQFKDVFL    50
    GFDKRPYSRA TTAQKCVRAG GKHNDLENVG YTARHHTFFE MMGNFSFGDY 100
    FKRDAIHFAW EFLTSPEWLN IPKDKLLATV YAEDDEAYNI WLNEIGMPSE 150
    RIVRIGDNKG AKYVSDNFWQ MGDTGPCGPC SEIFYDHGEE IWGGIPGSPE 200
    EDGDRWIEIW NCVFMQFNRD EQGNMNPLPK PSVDTGMGLE RMAAVMQHVH 250
    SNYEIDLFQD LLKAVARETG AAFSMDEPSL KVIADHIRSC SFLIADGVLP 300
    SNEGRGYVLR RIIRRAVRHG YKLGQSKPFF HKLVADLVKE MGGAYPELKE 350
    KQAQIEEALK NEESRFAQTL ETGMALLENA LAKGGKTLDG EIIFKLYDTY 400
    GFPYDLTADI CRERNIELDE AGFEREMEAQ RARARAAQSF KANAQLPYDG 450
    QDTEFKGYSE RQTESKVLAL YKDGEQVNEL NEGDSGAVVI DFTPFYAESG 500
    GQVGDVGYIF SSENRFEVRD TQKIKAAVFG QFGVQTSGRL KVGDSVTAKV 550
    DDEIRNANMR NHSATHLMHK ALRDVLGGHV EQKGSLVTAE STRFDISHPQ 600
    AVTAEEIAEV ERRVNEAVLA NVAVNAAIMS MEDAQKTGAM MLFGEKYGEE 650
    VRVLQMGGFS TELCGGTHVS RTGDIGLFKI ISEGGIAAGV RRIEAITGLN 700
    ALKWAQEQER LVKDIIAETK AQTEKDVLAK IQAGAAHAKA LEKELARAKA 750
    ELAVHAGAKL LDDAKDLGAA KLVAAQIEAD AAALREIVTD LTGKSDNAVI 800
    LLAAVNDGKV SLCAGVSKPL TGKVKAGDLV KFAAEQVGGK GGGRPDLAQA 850
    GGTDAGKLPE MLVSVESWLC QKLS 874
    Length:874
    Mass (Da):96,032
    Last modified:October 1, 2000 - v1
    Checksum:i17EE6A14668211EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL157959 Genomic DNA. Translation: CAM08912.1.
    PIRiC81804.
    RefSeqiYP_002343076.1. NC_003116.1.

    Genome annotation databases

    EnsemblBacteriaiCAM08912; CAM08912; NMA1788.
    GeneIDi907637.
    KEGGinma:NMA1788.
    PATRICi20364887. VBINeiMen132687_2139.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL157959 Genomic DNA. Translation: CAM08912.1 .
    PIRi C81804.
    RefSeqi YP_002343076.1. NC_003116.1.

    3D structure databases

    ProteinModelPortali Q9JTG4.
    SMRi Q9JTG4. Positions 1-460.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 122587.NMA1788.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAM08912 ; CAM08912 ; NMA1788 .
    GeneIDi 907637.
    KEGGi nma:NMA1788.
    PATRICi 20364887. VBINeiMen132687_2139.

    Phylogenomic databases

    eggNOGi COG0013.
    HOGENOMi HOG000156964.
    KOi K01872.
    OMAi KYHTATH.
    OrthoDBi EOG6Q2SQ2.

    Enzyme and pathway databases

    BioCyci NMEN122587:GI3Q-1636-MONOMER.

    Family and domain databases

    HAMAPi MF_00036_B. Ala_tRNA_synth_B.
    InterProi IPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00980. TRNASYNTHALA.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsi TIGR00344. alaS. 1 hit.
    PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Z2491.

    Entry informationi

    Entry nameiSYA_NEIMA
    AccessioniPrimary (citable) accession number: Q9JTG4
    Secondary accession number(s): A1IT00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3