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Q9JTG4

- SYA_NEIMA

UniProt

Q9JTG4 - SYA_NEIMA

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Protein
Alanine--tRNA ligase
Gene
alaS, NMA1788
Organism
Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi562 – 5621Zinc Reviewed prediction
Metal bindingi566 – 5661Zinc Reviewed prediction
Metal bindingi664 – 6641Zinc Reviewed prediction
Metal bindingi668 – 6681Zinc Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciNMEN122587:GI3Q-1636-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:NMA1788
OrganismiNeisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Taxonomic identifieri122587 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
ProteomesiUP000000626: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Alanine--tRNA ligaseUniRule annotation
PRO_0000075160Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi122587.NMA1788.

Structurei

3D structure databases

ProteinModelPortaliQ9JTG4.
SMRiQ9JTG4. Positions 1-460.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156964.
KOiK01872.
OMAiKYHTATH.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JTG4-1 [UniParc]FASTAAdd to Basket

« Hide

MKTSELRQKF LKFFETKGHT VVRSSSLVPH DDPTLLFTNA GMNQFKDVFL    50
GFDKRPYSRA TTAQKCVRAG GKHNDLENVG YTARHHTFFE MMGNFSFGDY 100
FKRDAIHFAW EFLTSPEWLN IPKDKLLATV YAEDDEAYNI WLNEIGMPSE 150
RIVRIGDNKG AKYVSDNFWQ MGDTGPCGPC SEIFYDHGEE IWGGIPGSPE 200
EDGDRWIEIW NCVFMQFNRD EQGNMNPLPK PSVDTGMGLE RMAAVMQHVH 250
SNYEIDLFQD LLKAVARETG AAFSMDEPSL KVIADHIRSC SFLIADGVLP 300
SNEGRGYVLR RIIRRAVRHG YKLGQSKPFF HKLVADLVKE MGGAYPELKE 350
KQAQIEEALK NEESRFAQTL ETGMALLENA LAKGGKTLDG EIIFKLYDTY 400
GFPYDLTADI CRERNIELDE AGFEREMEAQ RARARAAQSF KANAQLPYDG 450
QDTEFKGYSE RQTESKVLAL YKDGEQVNEL NEGDSGAVVI DFTPFYAESG 500
GQVGDVGYIF SSENRFEVRD TQKIKAAVFG QFGVQTSGRL KVGDSVTAKV 550
DDEIRNANMR NHSATHLMHK ALRDVLGGHV EQKGSLVTAE STRFDISHPQ 600
AVTAEEIAEV ERRVNEAVLA NVAVNAAIMS MEDAQKTGAM MLFGEKYGEE 650
VRVLQMGGFS TELCGGTHVS RTGDIGLFKI ISEGGIAAGV RRIEAITGLN 700
ALKWAQEQER LVKDIIAETK AQTEKDVLAK IQAGAAHAKA LEKELARAKA 750
ELAVHAGAKL LDDAKDLGAA KLVAAQIEAD AAALREIVTD LTGKSDNAVI 800
LLAAVNDGKV SLCAGVSKPL TGKVKAGDLV KFAAEQVGGK GGGRPDLAQA 850
GGTDAGKLPE MLVSVESWLC QKLS 874
Length:874
Mass (Da):96,032
Last modified:October 1, 2000 - v1
Checksum:i17EE6A14668211EA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL157959 Genomic DNA. Translation: CAM08912.1.
PIRiC81804.
RefSeqiYP_002343076.1. NC_003116.1.

Genome annotation databases

EnsemblBacteriaiCAM08912; CAM08912; NMA1788.
GeneIDi907637.
KEGGinma:NMA1788.
PATRICi20364887. VBINeiMen132687_2139.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL157959 Genomic DNA. Translation: CAM08912.1 .
PIRi C81804.
RefSeqi YP_002343076.1. NC_003116.1.

3D structure databases

ProteinModelPortali Q9JTG4.
SMRi Q9JTG4. Positions 1-460.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 122587.NMA1788.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAM08912 ; CAM08912 ; NMA1788 .
GeneIDi 907637.
KEGGi nma:NMA1788.
PATRICi 20364887. VBINeiMen132687_2139.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156964.
KOi K01872.
OMAi KYHTATH.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci NMEN122587:GI3Q-1636-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z2491.

Entry informationi

Entry nameiSYA_NEIMA
AccessioniPrimary (citable) accession number: Q9JTG4
Secondary accession number(s): A1IT00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi