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Q9JT70 (DHPS_NEIMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Dihydropteroate synthase

Short name=DHPS
EC=2.5.1.15
Alternative name(s):
Dihydropteroate pyrophosphorylase
Gene names
Name:folP
Synonyms:dhpS
Ordered Locus Names:NMA1950
OrganismNeisseria meningitidis serogroup A / serotype 4A (strain Z2491) [Complete proteome] [HAMAP]
Taxonomic identifier122587 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria

Protein attributes

Sequence length283 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DHPS catalyzes the formation of the immediate precursor of folic acid. It is implicated in resistance to sulfonamide.

Catalytic activity

(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate.

Cofactor

Binds 1 magnesium ion per subunit. Magnesium is required for activity, even if it interacts primarily with the substrate By similarity.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 7,8-dihydrofolate from 2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate and 4-aminobenzoate: step 1/2.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the DHPS family.

Contains 1 pterin-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 283283Dihydropteroate synthase
PRO_0000168217

Regions

Domain18 – 274257Pterin-binding
Region66 – 672Substrate binding By similarity

Sites

Metal binding251Magnesium By similarity
Binding site331Substrate By similarity
Binding site991Substrate By similarity
Binding site1191Substrate By similarity
Binding site1901Substrate By similarity
Binding site2271Substrate By similarity
Binding site2621Substrate By similarity
Binding site2641Substrate By similarity

Natural variations

Natural variant2 – 43VGC → ARH in strain: 1014 and 418.
Natural variant311L → F in strain: 1014 and 418.
Natural variant681P → L in strain: 418.
Natural variant751P → S in strain: 1014.
Natural variant841S → P in strain: 1014 and 418.
Natural variant871A → E in strain: 418.
Natural variant871A → V in strain: 1014.
Natural variant1021H → R in strain: 1014 and 418.
Natural variant1051V → I in strain: 418.
Natural variant1151I → V in strain: 418.
Natural variant1251T → N in strain: 418.
Natural variant1301L → V in strain: 418.
Natural variant1351C → R in strain: 418.
Natural variant1471Q → R in strain: 1014.
Natural variant1511K → E in strain: 1014.
Natural variant1521N → T in strain: 418.
Natural variant1741A → S in strain: 418.
Natural variant1881T → I in strain: 418.
Natural variant1941C → G in strain: 1014.
Natural variant1941C → GSG in strain: 418.
Natural variant1981T → P in strain: 418.
Natural variant2041T → A in strain: 1014 and 418.
Natural variant2181Y → F in strain: 1014 and 418.
Natural variant2281S → R in strain: 1014.
Natural variant2291M → T in strain: 418.
Natural variant2301I → V in strain: 1014.
Natural variant237 – 2382TD → AN in strain: 418.
Natural variant2411A → E in strain: 418.
Natural variant2431G → V in strain: 1014 and 418.
Natural variant2531A → S in strain: 1014 and 418.
Natural variant2551A → R in strain: 1014.
Natural variant2591K → Q in strain: 1014 and 418.
Natural variant2751A → V in strain: 1014 and 418.

Sequences

Sequence LengthMass (Da)Tools
Q9JT70 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 89A0976A527C9E27

FASTA28329,928
        10         20         30         40         50         60 
MVGCVWQAGR FEIGLDKPKI MGIVNLTPDS LSDGGAYSQN AQTALAHAER LLKEGADILD 

        70         80         90        100        110        120 
IGGESTRPGA DYVSPEEEWA RVESVLAEVA GWGVPVSLDT RHTVVMEKAL ALGGIDIIND 

       130        140        150        160        170        180 
VAALTDEGAL ELLACQADTG ICLMHMQGLP KNMQINPKYQ DVVGEVARYL KARAAECIAA 

       190        200        210        220        230        240 
GIAPQRITLD PGFCFGKTLQ HNITLMRHLP ELMAETGYPL LIGVSRKSMI GELTGETDAA 

       250        260        270        280 
ARGHGSVAAA LAAVARGAKI VRVHDVKATA DALKAWEALG INL 

« Hide

References

« Hide 'large scale' references
[1]"Transformational exchanges in the dihydropteroate synthase gene of Neisseria meningitidis: a novel mechanism for acquisition of sulfonamide resistance."
Raadstroem P., Fermer C., Kristiansen B.-E., Jenkins A., Skoeld O., Swedberg G.
J. Bacteriol. 174:6386-6393(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1014 / Serogroup A / Serotype 4/21 and 418 / Serogroup A / Serotype 4/21.
[2]"Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491."
Parkhill J., Achtman M., James K.D., Bentley S.D., Churcher C.M., Klee S.R., Morelli G., Basham D., Brown D., Chillingworth T., Davies R.M., Davis P., Devlin K., Feltwell T., Hamlin N., Holroyd S., Jagels K., Leather S. expand/collapse author list , Moule S., Mungall K.L., Quail M.A., Rajandream M.A., Rutherford K.M., Simmonds M., Skelton J., Whitehead S., Spratt B.G., Barrell B.G.
Nature 404:502-506(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Z2491.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X68065 Genomic DNA. Translation: CAA48202.1.
X68068 Genomic DNA. Translation: CAA48205.1.
AL157959 Genomic DNA. Translation: CAM09062.1.
PIRD81823.
S25610.
S65838.
RefSeqYP_002343223.1. NC_003116.1.

3D structure databases

ProteinModelPortalQ9JT70.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING122587.NMA1950.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAM09062; CAM09062; NMA1950.
GeneID907184.
KEGGnma:NMA1950.
PATRIC20365276. VBINeiMen132687_2326.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0294.
HOGENOMHOG000217510.
KOK00796.
OMARIMLDPG.
OrthoDBEOG67T5P5.

Enzyme and pathway databases

BioCycNMEN122587:GI3Q-1793-MONOMER.
UniPathwayUPA00077; UER00156.

Family and domain databases

Gene3D3.20.20.20. 1 hit.
InterProIPR006390. DHP_synth.
IPR011005. Dihydropteroate_synth-like.
IPR000489. Pterin-binding.
[Graphical view]
PfamPF00809. Pterin_bind. 1 hit.
[Graphical view]
SUPFAMSSF51717. SSF51717. 1 hit.
TIGRFAMsTIGR01496. DHPS. 1 hit.
PROSITEPS00792. DHPS_1. 1 hit.
PS00793. DHPS_2. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHPS_NEIMA
AccessionPrimary (citable) accession number: Q9JT70
Secondary accession number(s): A1ITE8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: October 1, 2000
Last modified: May 14, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways