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Protein

Dihydrofolate reductase

Gene

folA

Organism
Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29SubstrateBy similarity1
Binding sitei60SubstrateBy similarity1
Binding sitei117SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 9NADPBy similarity2
Nucleotide bindingi16 – 21NADPBy similarity6
Nucleotide bindingi45 – 48NADPBy similarity4
Nucleotide bindingi65 – 68NADPBy similarity4
Nucleotide bindingi98 – 103NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Ordered Locus Names:NMA2179
OrganismiNeisseria meningitidis serogroup A / serotype 4A (strain Z2491)
Taxonomic identifieri122587 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaNeisserialesNeisseriaceaeNeisseria
Proteomesi
  • UP000000626 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001864021 – 162Dihydrofolate reductaseAdd BLAST162

Proteomic databases

PRIDEiQ9JSQ9

Structurei

3D structure databases

ProteinModelPortaliQ9JSQ9
SMRiQ9JSQ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 161DHFRPROSITE-ProRule annotationAdd BLAST159

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 9Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

HOGENOMiHOG000040234
KOiK00287
OMAiRDNQLPW

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.101 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
PIRSFiPIRSF000194 DHFR, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JSQ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKITLIAAC AENLCIGAGN AMPWHIPEDF AFFKAYTLGK PVIMGRKTWE
60 70 80 90 100
SLPVKPLPGR RNIVISRQAD YCAAGAETAA SLEAALALCA GAEEAVIMGG
110 120 130 140 150
AQIYGQAMPL ATDLRITEVD LSVEGDAFFP AIDRTHWKEA ERTERRVSSK
160
GTSYAFVHYL RY
Length:162
Mass (Da):17,682
Last modified:October 1, 2000 - v1
Checksum:iB5EAF03CEABB8F62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL157959 Genomic DNA Translation: CAM09274.1
PIRiH81790
RefSeqiWP_002216373.1, NC_003116.1

Genome annotation databases

EnsemblBacteriaiCAM09274; CAM09274; NMA2179
KEGGinma:NMA2179

Similar proteinsi

Entry informationi

Entry nameiDYR_NEIMA
AccessioniPrimary (citable) accession number: Q9JSQ9
Secondary accession number(s): A1IU05
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome