Reviewed, UniProtKB/Swiss-Prot Q9JRT1 (CYSH_NEIMB)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH1
Ordered Locus Names:	NMB1155
AND
Name:	cysH2
Ordered Locus Names:	NMB1193

Organism

Neisseria meningitidis serogroup B [Complete proteome] [HAMAP]

Taxonomic identifier

491 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Neisseriales › Neisseriaceae › Neisseria

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_0000100636

Sequences

	Sequence		Length	Mass (Da)
	Q9JRT1-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FAD0206EF23FEB98	FASTA	246	28,091
	10 20 30 40 50 60 METTLFKPAL WQIPHIGSGG ETALAEKTET LKQRLHRIVG SHRDARFASS LAAEDMVITD 70 80 90 100 110 120 LIAGENLNIG IFTLDTGLLH TETLNLLDRL GRAYPHLRIK RFRPVREDAD RYVESKGRFA 130 140 150 160 170 180 FYDSVEARRE CCRIRKTEPL NRAIAGADAW LTGQRREQSA TRTELPFAEY DAGRGIGKYN 190 200 210 220 230 240 PIFDWSEHDV WAYILANNVP YNDLYRQGFP SIGCDPCTRP VKAGEDIRAG RWWWEGRNSK ECGLHK

References

[1]

"Complete genome sequence of Neisseria meningitidis serogroup B strain MC58." Tettelin H., Saunders N.J., Heidelberg J.F., Jeffries A.C., Nelson K.E., Eisen J.A., Ketchum K.A., Hood D.W., Peden J.F., Dodson R.J., Nelson W.C., Gwinn M.L., DeBoy R.T., Peterson J.D., Hickey E.K., Haft D.H., Salzberg S.L., White O. , Fleischmann R.D., Dougherty B.A., Mason T.M., Ciecko A., Parksey D.S., Blair E., Cittone H., Clark E.B., Cotton M.D., Utterback T.R., Khouri H.M., Qin H., Vamathevan J.J., Gill J., Scarlato V., Masignani V., Pizza M., Grandi G., Sun L., Smith H.O., Fraser C.M., Moxon E.R., Rappuoli R., Venter J.C. Science 287:1809-1815(2000) [PubMed: 10710307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MC58 / Serogroup B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE002098 Genomic DNA. Translation: AAF41541.1. AE002098 Genomic DNA. Translation: AAF41576.1.
PIR	C81111.
RefSeq	NP_274183.1. NP_274219.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	903576. 903613.
GenomeReviews	Gene locus NMB1155 in contig AE002098_GR. Gene locus NMB1193 in contig AE002098_GR.
KEGG	nme:NMB1155. nme:NMB1193.
TIGR	NMB1155. NMB1193.
Phylogenomic databases
HOGENOM	Q9JRT1.
OMA	Q9JRT1. WWWENPE.
Enzyme and pathway databases
BioCyc	NMEN122586:NMB_1155-MON. NMEN122586:NMB_1193-MON.
BRENDA	1.8.4.8. 293828.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_NEIMB

Accession

Primary (citable) accession number: Q9JRT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 22, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)