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Q9JN65

- TOP1_MYCGA

UniProt

Q9JN65 - TOP1_MYCGA

Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (15 Aug 2003)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi12 – 121Magnesium 1; catalyticUniRule annotation
    Sitei36 – 361Interaction with DNAUniRule annotation
    Metal bindingi106 – 1061Magnesium 1; catalyticUniRule annotation
    Metal bindingi106 – 1061Magnesium 2UniRule annotation
    Metal bindingi108 – 1081Magnesium 2UniRule annotation
    Sitei166 – 1661Interaction with DNAUniRule annotation
    Sitei167 – 1671Interaction with DNAUniRule annotation
    Sitei170 – 1701Interaction with DNAUniRule annotation
    Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei336 – 3361Interaction with DNAUniRule annotation
    Sitei532 – 5321Interaction with DNAUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri620 – 64627C4-type 1Add
    BLAST
    Zinc fingeri667 – 69933C4-type 2Add
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGAL710127:GC09-715-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:MYCGA6340
    ORF Names:MGA_0454
    OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
    Taxonomic identifieri710127 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000001418: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 719719DNA topoisomerase 1PRO_0000145154Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi233150.MGA_0454.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JN65.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini6 – 137132ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni189 – 1946Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri620 – 64627C4-type 1Add
    BLAST
    Zinc fingeri667 – 69933C4-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0551.
    HOGENOMiHOG000004018.
    KOiK03168.
    OMAiVVECDKC.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JN65-1 [UniParc]FASTAAdd to Basket

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    MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE    50
    DFVPKWEIIQ EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE 100
    IYLATDPDRE GEAISWHVYD VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA 150
    NPREIQKDWV SSQITRRRLD RLIGFKLSSL MKSKINADSA GRVQSIALKF 200
    ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD KLGFKELEEV 250
    SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA 300
    INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV 350
    LDFVAKTYGK DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD 400
    SVKNKISSDQ YSLYKLIWTR TVAHYMAAAV YELVNIRFIN NNNKFYAVNN 450
    TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI DKEFEAKDVL LNEHQTKPPA 500
    RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG RYYKTTDLGQ 550
    KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV 600
    LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE 650
    KSEQKAKEIV YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK 700
    YTKSLEPEKD ETIRDSTII 719
    Length:719
    Mass (Da):82,773
    Last modified:August 15, 2003 - v2
    Checksum:iF9699B867C942796
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti68 – 681V → T in AAF36767. 1 PublicationCurated
    Sequence conflicti244 – 2441F → Y in AAF36767. 1 PublicationCurated
    Sequence conflicti656 – 6561A → P in AAF36767. 1 PublicationCurated
    Sequence conflicti666 – 6661K → E in AAF36767. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35043 Genomic DNA. Translation: AAF36767.1.
    AE015450 Genomic DNA. Translation: AAP56984.1.
    RefSeqiNP_853416.1. NC_004829.2.

    Genome annotation databases

    EnsemblBacteriaiAAP56984; AAP56984; MGA_0454.
    GeneIDi1089951.
    KEGGimga:MGA_0454.
    PATRICi20009163. VBIMycGal115357_0711.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L35043 Genomic DNA. Translation: AAF36767.1 .
    AE015450 Genomic DNA. Translation: AAP56984.1 .
    RefSeqi NP_853416.1. NC_004829.2.

    3D structure databases

    ProteinModelPortali Q9JN65.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233150.MGA_0454.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAP56984 ; AAP56984 ; MGA_0454 .
    GeneIDi 1089951.
    KEGGi mga:MGA_0454.
    PATRICi 20009163. VBIMycGal115357_0711.

    Phylogenomic databases

    eggNOGi COG0551.
    HOGENOMi HOG000004018.
    KOi K03168.
    OMAi VVECDKC.
    OrthoDBi EOG6S7XQ9.

    Enzyme and pathway databases

    BioCyci MGAL710127:GC09-715-MONOMER.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR013498. Topo_IA_Znf.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF01396. zf-C4_Topoisom. 2 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Skamrov A.V., Feoktistova E.S., Gol'dman M.A., Bibilashvili R.S.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A5969Var.B.
    2. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
      Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
      Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: R(low / passage 15 / clone 2).
    3. "Mycoplasma gallisepticum strain S6 genome contains three regions hybridizing with 16 S rRNA and two regions hybridizing with 23 S and 5 S rRNA."
      Skamrov A.V., Bibilashvili R.S.
      FEBS Lett. 291:71-74(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-719.
      Strain: A5969Var.B.

    Entry informationi

    Entry nameiTOP1_MYCGA
    AccessioniPrimary (citable) accession number: Q9JN65
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2001
    Last sequence update: August 15, 2003
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3