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Q9JN65 (TOP1_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:MYCGA6340
ORF Names:MGA_0454
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length719 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Ontologies

Keywords
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Magnesium
Metal-binding
Zinc
   Molecular functionIsomerase
Topoisomerase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 719719DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145154

Regions

Domain6 – 137132Toprim
Zinc finger620 – 64627C4-type 1 HAMAP-Rule MF_00952
Zinc finger667 – 69933C4-type 2 HAMAP-Rule MF_00952
Region189 – 1946Interaction with DNA By similarity

Sites

Active site3341O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding121Magnesium 1; catalytic By similarity
Metal binding1061Magnesium 1; catalytic By similarity
Metal binding1061Magnesium 2 By similarity
Metal binding1081Magnesium 2 By similarity
Site361Interaction with DNA By similarity
Site1661Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site1701Interaction with DNA By similarity
Site3361Interaction with DNA By similarity
Site5321Interaction with DNA By similarity

Experimental info

Sequence conflict681V → T in AAF36767. Ref.1
Sequence conflict2441F → Y in AAF36767. Ref.1
Sequence conflict6561A → P in AAF36767. Ref.1
Sequence conflict6661K → E in AAF36767. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JN65 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: F9699B867C942796

FASTA71982,773
        10         20         30         40         50         60 
MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE DFVPKWEIIQ 

        70         80         90        100        110        120 
EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE IYLATDPDRE GEAISWHVYD 

       130        140        150        160        170        180 
VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA NPREIQKDWV SSQITRRRLD RLIGFKLSSL 

       190        200        210        220        230        240 
MKSKINADSA GRVQSIALKF ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD 

       250        260        270        280        290        300 
KLGFKELEEV SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA 

       310        320        330        340        350        360 
INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV LDFVAKTYGK 

       370        380        390        400        410        420 
DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD SVKNKISSDQ YSLYKLIWTR 

       430        440        450        460        470        480 
TVAHYMAAAV YELVNIRFIN NNNKFYAVNN TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI 

       490        500        510        520        530        540 
DKEFEAKDVL LNEHQTKPPA RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG 

       550        560        570        580        590        600 
RYYKTTDLGQ KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV 

       610        620        630        640        650        660 
LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE KSEQKAKEIV 

       670        680        690        700        710 
YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK YTKSLEPEKD ETIRDSTII 

« Hide

References

« Hide 'large scale' references
[1]Skamrov A.V., Feoktistova E.S., Gol'dman M.A., Bibilashvili R.S.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A5969Var.B.
[2]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).
[3]"Mycoplasma gallisepticum strain S6 genome contains three regions hybridizing with 16 S rRNA and two regions hybridizing with 23 S and 5 S rRNA."
Skamrov A.V., Bibilashvili R.S.
FEBS Lett. 291:71-74(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-719.
Strain: A5969Var.B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L35043 Genomic DNA. Translation: AAF36767.1.
AE015450 Genomic DNA. Translation: AAP56984.1.
RefSeqNP_853416.1. NC_004829.2.

3D structure databases

ProteinModelPortalQ9JN65.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_0454.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP56984; AAP56984; MGA_0454.
GeneID1089951.
KEGGmga:MGA_0454.
PATRIC20009163. VBIMycGal115357_0711.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAVVECDKC.
OrthoDBEOG6S7XQ9.

Enzyme and pathway databases

BioCycMGAL710127:GC09-715-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_MYCGA
AccessionPrimary (citable) accession number: Q9JN65
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 15, 2003
Last modified: July 9, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families