SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9JN65

- TOP1_MYCGA

UniProt

Q9JN65 - TOP1_MYCGA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA topoisomerase 1

Gene
topA, MYCGA6340, MGA_0454
Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Magnesium 1; catalytic By similarity
Sitei36 – 361Interaction with DNA By similarity
Metal bindingi106 – 1061Magnesium 1; catalytic By similarity
Metal bindingi106 – 1061Magnesium 2 By similarity
Metal bindingi108 – 1081Magnesium 2 By similarity
Sitei166 – 1661Interaction with DNA By similarity
Sitei167 – 1671Interaction with DNA By similarity
Sitei170 – 1701Interaction with DNA By similarity
Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei336 – 3361Interaction with DNA By similarity
Sitei532 – 5321Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri620 – 64627C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri667 – 69933C4-type 2UniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:GC09-715-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:MYCGA6340
ORF Names:MGA_0454
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719DNA topoisomerase 1UniRule annotationPRO_0000145154Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233150.MGA_0454.

Structurei

3D structure databases

ProteinModelPortaliQ9JN65.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 137132ToprimAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1946Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri620 – 64627C4-type 1UniRule annotationAdd
BLAST
Zinc fingeri667 – 69933C4-type 2UniRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JN65-1 [UniParc]FASTAAdd to Basket

« Hide

MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE    50
DFVPKWEIIQ EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE 100
IYLATDPDRE GEAISWHVYD VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA 150
NPREIQKDWV SSQITRRRLD RLIGFKLSSL MKSKINADSA GRVQSIALKF 200
ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD KLGFKELEEV 250
SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA 300
INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV 350
LDFVAKTYGK DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD 400
SVKNKISSDQ YSLYKLIWTR TVAHYMAAAV YELVNIRFIN NNNKFYAVNN 450
TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI DKEFEAKDVL LNEHQTKPPA 500
RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG RYYKTTDLGQ 550
KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV 600
LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE 650
KSEQKAKEIV YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK 700
YTKSLEPEKD ETIRDSTII 719
Length:719
Mass (Da):82,773
Last modified:August 15, 2003 - v2
Checksum:iF9699B867C942796
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681V → T in AAF36767. 1 Publication
Sequence conflicti244 – 2441F → Y in AAF36767. 1 Publication
Sequence conflicti656 – 6561A → P in AAF36767. 1 Publication
Sequence conflicti666 – 6661K → E in AAF36767. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35043 Genomic DNA. Translation: AAF36767.1.
AE015450 Genomic DNA. Translation: AAP56984.1.
RefSeqiNP_853416.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56984; AAP56984; MGA_0454.
GeneIDi1089951.
KEGGimga:MGA_0454.
PATRICi20009163. VBIMycGal115357_0711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L35043 Genomic DNA. Translation: AAF36767.1 .
AE015450 Genomic DNA. Translation: AAP56984.1 .
RefSeqi NP_853416.1. NC_004829.2.

3D structure databases

ProteinModelPortali Q9JN65.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233150.MGA_0454.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAP56984 ; AAP56984 ; MGA_0454 .
GeneIDi 1089951.
KEGGi mga:MGA_0454.
PATRICi 20009163. VBIMycGal115357_0711.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci MGAL710127:GC09-715-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Skamrov A.V., Feoktistova E.S., Gol'dman M.A., Bibilashvili R.S.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A5969Var.B.
  2. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).
  3. "Mycoplasma gallisepticum strain S6 genome contains three regions hybridizing with 16 S rRNA and two regions hybridizing with 23 S and 5 S rRNA."
    Skamrov A.V., Bibilashvili R.S.
    FEBS Lett. 291:71-74(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-719.
    Strain: A5969Var.B.

Entry informationi

Entry nameiTOP1_MYCGA
AccessioniPrimary (citable) accession number: Q9JN65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 15, 2003
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi