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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Magnesium 1; catalyticUniRule annotation
Sitei36 – 361Interaction with DNAUniRule annotation
Metal bindingi106 – 1061Magnesium 1; catalyticUniRule annotation
Metal bindingi106 – 1061Magnesium 2UniRule annotation
Metal bindingi108 – 1081Magnesium 2UniRule annotation
Sitei166 – 1661Interaction with DNAUniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei170 – 1701Interaction with DNAUniRule annotation
Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei336 – 3361Interaction with DNAUniRule annotation
Sitei532 – 5321Interaction with DNAUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri620 – 64627C4-type 1Add
BLAST
Zinc fingeri667 – 69933C4-type 2Add
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:GC09-715-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MYCGA6340
ORF Names:MGA_0454
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719DNA topoisomerase 1PRO_0000145154Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi233150.MGA_0454.

Structurei

3D structure databases

ProteinModelPortaliQ9JN65.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 137132ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni189 – 1946Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri620 – 64627C4-type 1Add
BLAST
Zinc fingeri667 – 69933C4-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiPNQHFTE.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JN65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE
60 70 80 90 100
DFVPKWEIIQ EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE
110 120 130 140 150
IYLATDPDRE GEAISWHVYD VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA
160 170 180 190 200
NPREIQKDWV SSQITRRRLD RLIGFKLSSL MKSKINADSA GRVQSIALKF
210 220 230 240 250
ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD KLGFKELEEV
260 270 280 290 300
SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA
310 320 330 340 350
INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV
360 370 380 390 400
LDFVAKTYGK DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD
410 420 430 440 450
SVKNKISSDQ YSLYKLIWTR TVAHYMAAAV YELVNIRFIN NNNKFYAVNN
460 470 480 490 500
TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI DKEFEAKDVL LNEHQTKPPA
510 520 530 540 550
RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG RYYKTTDLGQ
560 570 580 590 600
KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV
610 620 630 640 650
LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE
660 670 680 690 700
KSEQKAKEIV YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK
710
YTKSLEPEKD ETIRDSTII
Length:719
Mass (Da):82,773
Last modified:August 15, 2003 - v2
Checksum:iF9699B867C942796
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti68 – 681V → T in AAF36767 (Ref. 1) Curated
Sequence conflicti244 – 2441F → Y in AAF36767 (Ref. 1) Curated
Sequence conflicti656 – 6561A → P in AAF36767 (Ref. 1) Curated
Sequence conflicti666 – 6661K → E in AAF36767 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35043 Genomic DNA. Translation: AAF36767.1.
AE015450 Genomic DNA. Translation: AAP56984.1.
RefSeqiNP_853416.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56984; AAP56984; MGA_0454.
KEGGimga:MGA_0454.
PATRICi20009163. VBIMycGal115357_0711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35043 Genomic DNA. Translation: AAF36767.1.
AE015450 Genomic DNA. Translation: AAP56984.1.
RefSeqiNP_853416.1. NC_004829.2.

3D structure databases

ProteinModelPortaliQ9JN65.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233150.MGA_0454.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56984; AAP56984; MGA_0454.
KEGGimga:MGA_0454.
PATRICi20009163. VBIMycGal115357_0711.

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiPNQHFTE.
OrthoDBiEOG6S7XQ9.

Enzyme and pathway databases

BioCyciMGAL710127:GC09-715-MONOMER.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR013498. Topo_IA_Znf.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF01396. zf-C4_Topoisom. 2 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Skamrov A.V., Feoktistova E.S., Gol'dman M.A., Bibilashvili R.S.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A5969Var.B.
  2. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).
  3. "Mycoplasma gallisepticum strain S6 genome contains three regions hybridizing with 16 S rRNA and two regions hybridizing with 23 S and 5 S rRNA."
    Skamrov A.V., Bibilashvili R.S.
    FEBS Lett. 291:71-74(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-719.
    Strain: A5969Var.B.

Entry informationi

Entry nameiTOP1_MYCGA
AccessioniPrimary (citable) accession number: Q9JN65
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 15, 2003
Last modified: April 1, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.