DNA topoisomerase 1 - Mycoplasma gallisepticum

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Reviewed, UniProtKB/Swiss-Prot Q9JN65 (TOP1_MYCGA)

Last modified November 3, 2009. Version 66. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	MYCGA6340
ORF Names:	MGA_0454

Organism

Mycoplasma gallisepticum [Complete proteome] [HAMAP]

Taxonomic identifier

2096 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

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Protein attributes

Sequence length	719 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

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General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

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Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 719

719

DNA topoisomerase 1

PRO_0000145154

Regions

Zinc finger

620 – 646

C4-type 1

Zinc finger

667 – 699

C4-type 2

Sites

Active site

334

For DNA cleavage activity By similarity

Experimental info

Sequence conflict

V → T in AAF36767. Ref.1

Sequence conflict

244

F → Y in AAF36767. Ref.1

Sequence conflict

656

A → P in AAF36767. Ref.1

Sequence conflict

666

K → E in AAF36767. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9JN65-1 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: F9699B867C942796
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FASTA

719

82,773

        10         20         30         40         50         60 
MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE DFVPKWEIIQ 

        70         80         90        100        110        120 
EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE IYLATDPDRE GEAISWHVYD 

       130        140        150        160        170        180 
VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA NPREIQKDWV SSQITRRRLD RLIGFKLSSL 

       190        200        210        220        230        240 
MKSKINADSA GRVQSIALKF ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD 

       250        260        270        280        290        300 
KLGFKELEEV SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA 

       310        320        330        340        350        360 
INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV LDFVAKTYGK 

       370        380        390        400        410        420 
DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD SVKNKISSDQ YSLYKLIWTR 

       430        440        450        460        470        480 
TVAHYMAAAV YELVNIRFIN NNNKFYAVNN TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI 

       490        500        510        520        530        540 
DKEFEAKDVL LNEHQTKPPA RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG 

       550        560        570        580        590        600 
RYYKTTDLGQ KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV 

       610        620        630        640        650        660 
LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE KSEQKAKEIV 

       670        680        690        700        710 
YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK YTKSLEPEKD ETIRDSTII

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Skamrov A.V., Feoktistova E.S., Gol'dman M.A., Bibilashvili R.S. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A5969Var.B.
[2]	"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)." Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J. Microbiology 149:2307-2316(2003) [PubMed: 12949158] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: R(low / passage 15 / clone 2).
[3]	"Mycoplasma gallisepticum strain S6 genome contains three regions hybridizing with 16 S rRNA and two regions hybridizing with 23 S and 5 S rRNA." Skamrov A.V., Bibilashvili R.S. FEBS Lett. 291:71-74(1991) [PubMed: 1718781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 706-719. Strain: A5969Var.B.

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Cross-references

Sequence databases
	L35043 Genomic DNA. Translation: AAF36767.1. AE015450 Genomic DNA. Translation: AAP56984.1.
RefSeq	NP_853416.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	1089951.
GenomeReviews	Gene locus MYCGA6340 in contig AE015450_GR.
KEGG	mga:MGA_0454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9JN65.
OMA	RKFYGCS.
Enzyme and pathway databases
BRENDA	5.99.1.2. 141882.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 1 hit. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 2 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

TOP1_MYCGA

Accession

Primary (citable) accession number: Q9JN65

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	August 15, 2003
Last modified:	November 3, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents