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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Magnesium 1; catalyticUniRule annotation1
Metal bindingi106Magnesium 1; catalyticUniRule annotation1
Metal bindingi106Magnesium 2UniRule annotation1
Metal bindingi108Magnesium 2UniRule annotation1
Active sitei334O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri620 – 646C4-type 1Add BLAST27
Zinc fingeri667 – 699C4-type 2Add BLAST33

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMGAL710127:G1GL5-735-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:MYCGA6340
ORF Names:MGA_0454
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451541 – 719DNA topoisomerase 1Add BLAST719

Proteomic databases

PRIDEiQ9JN65

Interactioni

Subunit structurei

Monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei36Interaction with DNAUniRule annotation1
Sitei166Interaction with DNAUniRule annotation1
Sitei167Interaction with DNAUniRule annotation1
Sitei170Interaction with DNAUniRule annotation1
Sitei336Interaction with DNAUniRule annotation1
Sitei532Interaction with DNAUniRule annotation1

Structurei

3D structure databases

ProteinModelPortaliQ9JN65
SMRiQ9JN65
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 137ToprimUniRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni189 – 194Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri620 – 646C4-type 1Add BLAST27
Zinc fingeri667 – 699C4-type 2Add BLAST33

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

HOGENOMiHOG000004018
KOiK03168
OMAiPECKYTR

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03363 TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
HAMAPiMF_00952 Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR013498 Topo_IA_Znf
IPR005733 TopoI_bac-type
IPR028612 Topoisom_1_IA
IPR006171 TOPRIM_domain
IPR034149 TOPRIM_TopoI
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PF01396 zf-C4_Topoisom, 2 hits
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
TIGRFAMsiTIGR01051 topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JN65-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNHSSKILV IESPNKVKTI KKYLTDDYEI VATVGHIRDL PKYTLGFNTE
60 70 80 90 100
DFVPKWEIIQ EKKPAKGVSA TKKAKTVKKA VKNKKEIIDE LVDKAKKADE
110 120 130 140 150
IYLATDPDRE GEAISWHVYD VLKEKGVNVN KCKRIIFNEI SEKAVKNAIA
160 170 180 190 200
NPREIQKDWV SSQITRRRLD RLIGFKLSSL MKSKINADSA GRVQSIALKF
210 220 230 240 250
ITEREELINK FVPRFWWTLD VILKDGTELM LRKIDEKLKD KLGFKELEEV
260 270 280 290 300
SGIDFNTKED AELVKQHLSD KYRVYAIDTP KLKSSYPKEV YKTSTLQQDA
310 320 330 340 350
INKLKWRSMK ITSVAQELYE GVTIDDEQIA LISYPRTDST RLSPEYGKTV
360 370 380 390 400
LDFVAKTYGK DYVATQSQLN GETKANKKQK AKVQDAHEAI HPIDISITPD
410 420 430 440 450
SVKNKISSDQ YSLYKLIWTR TVAHYMAAAV YELVNIRFIN NNNKFYAVNN
460 470 480 490 500
TLKFDGYKKI YTHYDDKDHL RKLDLNHFAI DKEFEAKDVL LNEHQTKPPA
510 520 530 540 550
RFTQATLIEA LETEGIGRPS TYSTILDIVL KRNYAELVNG RYYKTTDLGQ
560 570 580 590 600
KLAFELDKNF PTIINKEFTK NMETTLDEIA QGTVNDVSYL QAFWNDFSEV
610 620 630 640 650
LNEAKTNIVK KVEYIEGKNC PNCSSQLVRR EGRYGPFVGC SNFPKCKFIE
660 670 680 690 700
KSEQKAKEIV YEEGVKCELC HAKLIRRVAK KKGRNKDNTF LGCSNFPKCK
710
YTKSLEPEKD ETIRDSTII
Length:719
Mass (Da):82,773
Last modified:August 15, 2003 - v2
Checksum:iF9699B867C942796
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68V → T in AAF36767 (Ref. 1) Curated1
Sequence conflicti244F → Y in AAF36767 (Ref. 1) Curated1
Sequence conflicti656A → P in AAF36767 (Ref. 1) Curated1
Sequence conflicti666K → E in AAF36767 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L35043 Genomic DNA Translation: AAF36767.1
AE015450 Genomic DNA Translation: AAP56984.1
RefSeqiWP_011113893.1, NC_004829.2

Genome annotation databases

EnsemblBacteriaiAAP56984; AAP56984; MGA_0454
GeneIDi1089951
KEGGimga:MGA_0454
PATRICifig|233150.7.peg.711

Similar proteinsi

Entry informationi

Entry nameiTOP1_MYCGA
AccessioniPrimary (citable) accession number: Q9JN65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: August 15, 2003
Last modified: May 23, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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