Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Casein kinase I isoform epsilon

Gene

Csnk1e

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates DVL1. Central component of the circadian clock. In balance with PP1, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. Inhibits cytokine-induced granuloytic differentiation.5 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Enzyme regulationi

Phosphorylation leads to a decrease in the catalytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATPPROSITE-ProRule annotation1
Active sitei128Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 23ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cellular protein localization Source: MGI
  • cellular response to nerve growth factor stimulus Source: Ensembl
  • circadian behavior Source: Ensembl
  • circadian regulation of gene expression Source: UniProtKB
  • circadian rhythm Source: MGI
  • endocytosis Source: GO_Central
  • negative regulation of protein binding Source: MGI
  • negative regulation of Wnt signaling pathway Source: MGI
  • peptidyl-serine phosphorylation Source: MGI
  • positive regulation of beta-amyloid formation Source: Ensembl
  • positive regulation of canonical Wnt signaling pathway Source: MGI
  • positive regulation of non-canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
  • positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  • positive regulation of Wnt signaling pathway Source: ParkinsonsUK-UCL
  • protein phosphorylation Source: UniProtKB
  • regulation of cell shape Source: GO_Central
  • regulation of cellular protein localization Source: ParkinsonsUK-UCL
  • regulation of circadian rhythm Source: UniProtKB
  • Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Biological rhythms, Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.
ReactomeiR-MMU-201688. WNT mediated activation of DVL.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-400253. Circadian Clock.
R-MMU-508751. Circadian Clock.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I isoform epsilon (EC:2.7.11.1)
Short name:
CKI-epsilon
Short name:
CKIe
Gene namesi
Name:Csnk1e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1351660. Csnk1e.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • growth cone Source: Ensembl
  • intracellular ribonucleoprotein complex Source: MGI
  • neuronal cell body Source: Ensembl
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Has no apparent effect on circadian oscillation of protein levels. Mice exhibit a small but significant increase in circadian period length.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38K → A: Decreases PER1 phosphorylation. 1 Publication1
Mutagenesisi38K → R: Increases PER1 nuclear import. 1 Publication1
Mutagenesisi178R → C: Shortens circadian rhythm. Accelerates PER2 degradation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001928381 – 416Casein kinase I isoform epsilonAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1
Modified residuei354PhosphoserineBy similarity1
Modified residuei362PhosphothreonineBy similarity1
Modified residuei363PhosphoserineBy similarity1
Modified residuei382Omega-N-methylarginineCombined sources1
Modified residuei389PhosphoserineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei408PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated. Partially dephosphorylated by PPP5C. May be dephosphorylated by PP1.

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

MaxQBiQ9JMK2.
PaxDbiQ9JMK2.
PeptideAtlasiQ9JMK2.
PRIDEiQ9JMK2.

PTM databases

iPTMnetiQ9JMK2.
PhosphoSitePlusiQ9JMK2.

Expressioni

Tissue specificityi

Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. Expressed in monocytes and lymphocytes but not in granulocytes.1 Publication

Inductioni

Down-regulated during granulocytic differentiation. Does not show circadian oscillations.1 Publication

Gene expression databases

BgeeiENSMUSG00000022433.
CleanExiMM_CSNK1E.
ExpressionAtlasiQ9JMK2. baseline and differential.
GenevisibleiQ9JMK2. MM.

Interactioni

Subunit structurei

Monomer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARTNL/BMAL1 or ARTNL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts with ANKRD6, DBNDD2, LRP5, LRP6 and SOCS3. Interacts with SNAI1 (via zinc fingers).1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ClockO087852EBI-771709,EBI-79859

Protein-protein interaction databases

BioGridi205180. 49 interactors.
DIPiDIP-32410N.
IntActiQ9JMK2. 58 interactors.
STRINGi10090.ENSMUSP00000113341.

Structurei

3D structure databases

ProteinModelPortaliQ9JMK2.
SMRiQ9JMK2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini9 – 277Protein kinasePROSITE-ProRule annotationAdd BLAST269

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
HOVERGENiHBG000176.
InParanoidiQ9JMK2.
KOiK08960.
OrthoDBiEOG091G0AFG.
PhylomeDBiQ9JMK2.
TreeFamiTF300544.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JMK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH
60 70 80 90 100
IESKFYKMMQ GGVGIPSIKW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF
110 120 130 140 150
SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFST
260 270 280 290 300
YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGAARN
310 320 330 340 350
PEDVDRERRE HEREERMGQL RGSATRALPP GPPTGATANR LRSAAEPVAS
360 370 380 390 400
TPASRIQQTG NTSPRAISRA DRERKVSMRL HRGAPANVSS SDLTGRQEVS
410
RLAASQTSVP FDHLGK
Length:416
Mass (Da):47,322
Last modified:May 10, 2004 - v2
Checksum:i38CC5299BB9040D7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28N → D in BAA88107 (PubMed:10640823).Curated1
Sequence conflicti310E → G in BAA88107 (PubMed:10640823).Curated1
Sequence conflicti379R → G in BAA88107 (PubMed:10640823).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028736 mRNA. Translation: BAA88107.2.
BC026127 mRNA. Translation: AAH26127.1.
CCDSiCCDS27640.1.
PIRiS47616.
RefSeqiNP_001276827.1. NM_001289898.1.
NP_038795.3. NM_013767.6.
XP_017172146.1. XM_017316657.1.
XP_017172147.1. XM_017316658.1.
XP_017172148.1. XM_017316659.1.
UniGeneiMm.30199.

Genome annotation databases

EnsembliENSMUST00000117786; ENSMUSP00000113341; ENSMUSG00000022433.
ENSMUST00000120859; ENSMUSP00000113975; ENSMUSG00000022433.
GeneIDi27373.
KEGGimmu:27373.
UCSCiuc007wtl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028736 mRNA. Translation: BAA88107.2.
BC026127 mRNA. Translation: AAH26127.1.
CCDSiCCDS27640.1.
PIRiS47616.
RefSeqiNP_001276827.1. NM_001289898.1.
NP_038795.3. NM_013767.6.
XP_017172146.1. XM_017316657.1.
XP_017172147.1. XM_017316658.1.
XP_017172148.1. XM_017316659.1.
UniGeneiMm.30199.

3D structure databases

ProteinModelPortaliQ9JMK2.
SMRiQ9JMK2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205180. 49 interactors.
DIPiDIP-32410N.
IntActiQ9JMK2. 58 interactors.
STRINGi10090.ENSMUSP00000113341.

PTM databases

iPTMnetiQ9JMK2.
PhosphoSitePlusiQ9JMK2.

Proteomic databases

MaxQBiQ9JMK2.
PaxDbiQ9JMK2.
PeptideAtlasiQ9JMK2.
PRIDEiQ9JMK2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117786; ENSMUSP00000113341; ENSMUSG00000022433.
ENSMUST00000120859; ENSMUSP00000113975; ENSMUSG00000022433.
GeneIDi27373.
KEGGimmu:27373.
UCSCiuc007wtl.2. mouse.

Organism-specific databases

CTDi1454.
MGIiMGI:1351660. Csnk1e.

Phylogenomic databases

eggNOGiKOG1164. Eukaryota.
ENOG410XPGP. LUCA.
GeneTreeiENSGT00760000119040.
HOGENOMiHOG000182055.
HOVERGENiHBG000176.
InParanoidiQ9JMK2.
KOiK08960.
OrthoDBiEOG091G0AFG.
PhylomeDBiQ9JMK2.
TreeFamiTF300544.

Enzyme and pathway databases

BRENDAi2.7.11.1. 3474.
ReactomeiR-MMU-201688. WNT mediated activation of DVL.
R-MMU-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-MMU-380259. Loss of Nlp from mitotic centrosomes.
R-MMU-380270. Recruitment of mitotic centrosome proteins and complexes.
R-MMU-400253. Circadian Clock.
R-MMU-508751. Circadian Clock.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.
R-MMU-8854518. AURKA Activation by TPX2.

Miscellaneous databases

PROiQ9JMK2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022433.
CleanExiMM_CSNK1E.
ExpressionAtlasiQ9JMK2. baseline and differential.
GenevisibleiQ9JMK2. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKC1E_MOUSE
AccessioniPrimary (citable) accession number: Q9JMK2
Secondary accession number(s): Q8R389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 10, 2004
Last modified: November 2, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.