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Protein

Beta-1,4-galactosyltransferase 5

Gene

B4galt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi236 – 2361ManganeseBy similarity
Binding sitei264 – 2641UDP-alpha-D-galactoseBy similarity
Binding sitei296 – 2961UDP-alpha-D-galactoseBy similarity
Metal bindingi329 – 3291Manganese; via tele nitrogenBy similarity
Binding sitei340 – 3401N-acetyl-D-glucosamineBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_278772. O-linked glycosylation of mucins.
REACT_308816. N-Glycan antennae elongation.
REACT_346228. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 5 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 5
Short name:
Beta4Gal-T5
Short name:
b4Gal-T5
Alternative name(s):
Beta-1,4-GalT II
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
Gene namesi
Name:B4galt5
Synonyms:Bgt-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1927169. B4galt5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini36 – 388353LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Beta-1,4-galactosyltransferase 5PRO_0000080546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi114 ↔ 158By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi229 ↔ 248By similarity
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence Analysis
Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9JMK0.
PRIDEiQ9JMK0.

PTM databases

PhosphoSiteiQ9JMK0.

Expressioni

Tissue specificityi

Highest levels in heart, brain, liver and kidney with lower levels in spleen, lung and testis.1 Publication

Developmental stagei

In the brain, expression increases after birth.1 Publication

Gene expression databases

BgeeiQ9JMK0.
ExpressionAtlasiQ9JMK0. baseline and differential.
GenevestigatoriQ9JMK0.

Structurei

3D structure databases

ProteinModelPortaliQ9JMK0.
SMRiQ9JMK0. Positions 114-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1735UDP-alpha-D-galactose bindingBy similarity
Regioni208 – 2103UDP-alpha-D-galactose bindingBy similarity
Regioni235 – 2362UDP-alpha-D-galactose bindingBy similarity
Regioni298 – 3014N-acetyl-D-glucosamine bindingBy similarity
Regioni329 – 3302UDP-alpha-D-galactose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9JMK0.
KOiK09905.
OMAiLPYTEFF.
OrthoDBiEOG7060R0.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JMK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG
60 70 80 90 100
ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT
110 120 130 140 150
FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG
160 170 180 190 200
GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI
210 220 230 240 250
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ
260 270 280 290 300
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
310 320 330 340 350
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER
360 370 380
QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY
Length:388
Mass (Da):44,814
Last modified:July 27, 2011 - v2
Checksum:iFB3D6FBA2F8B3775
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921R → A in BAA94791 (PubMed:11145975).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004786 mRNA. Translation: BAA94791.1.
AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
CH466551 Genomic DNA. Translation: EDL06506.1.
CCDSiCCDS17098.1.
RefSeqiNP_062809.2. NM_019835.2.
UniGeneiMm.200886.
Mm.489868.

Genome annotation databases

EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
GeneIDi56336.
KEGGimmu:56336.
UCSCiuc008nzm.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004786 mRNA. Translation: BAA94791.1.
AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
CH466551 Genomic DNA. Translation: EDL06506.1.
CCDSiCCDS17098.1.
RefSeqiNP_062809.2. NM_019835.2.
UniGeneiMm.200886.
Mm.489868.

3D structure databases

ProteinModelPortaliQ9JMK0.
SMRiQ9JMK0. Positions 114-380.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSiteiQ9JMK0.

Proteomic databases

MaxQBiQ9JMK0.
PRIDEiQ9JMK0.

Protocols and materials databases

DNASUi56336.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
GeneIDi56336.
KEGGimmu:56336.
UCSCiuc008nzm.1. mouse.

Organism-specific databases

CTDi9334.
MGIiMGI:1927169. B4galt5.

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9JMK0.
KOiK09905.
OMAiLPYTEFF.
OrthoDBiEOG7060R0.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_278772. O-linked glycosylation of mucins.
REACT_308816. N-Glycan antennae elongation.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiB4galt5. mouse.
NextBioi312326.
PROiQ9JMK0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JMK0.
ExpressionAtlasiQ9JMK0. baseline and differential.
GenevestigatoriQ9JMK0.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
    Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
    J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiB4GT5_MOUSE
AccessioniPrimary (citable) accession number: Q9JMK0
Secondary accession number(s): A2A5T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: May 27, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.