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Q9JMK0

- B4GT5_MOUSE

UniProt

Q9JMK0 - B4GT5_MOUSE

Protein

Beta-1,4-galactosyltransferase 5

Gene

B4galt5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi236 – 2361ManganeseBy similarity
    Binding sitei264 – 2641UDP-alpha-D-galactoseBy similarity
    Binding sitei296 – 2961UDP-alpha-D-galactoseBy similarity
    Metal bindingi329 – 3291Manganese; via tele nitrogenBy similarity
    Binding sitei340 – 3401N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198517. O-linked glycosylation of mucins.
    REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 5 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 5
    Short name:
    Beta4Gal-T5
    Short name:
    b4Gal-T5
    Alternative name(s):
    Beta-1,4-GalT II
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
    Gene namesi
    Name:B4galt5
    Synonyms:Bgt-5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1927169. B4galt5.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 388388Beta-1,4-galactosyltransferase 5PRO_0000080546Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi90 – 901N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi114 ↔ 158By similarity
    Glycosylationi128 – 1281N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi229 ↔ 248By similarity
    Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi364 – 3641N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9JMK0.

    PTM databases

    PhosphoSiteiQ9JMK0.

    Expressioni

    Tissue specificityi

    Highest levels in heart, brain, liver and kidney with lower levels in spleen, lung and testis.1 Publication

    Developmental stagei

    In the brain, expression increases after birth.1 Publication

    Gene expression databases

    ArrayExpressiQ9JMK0.
    BgeeiQ9JMK0.
    GenevestigatoriQ9JMK0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JMK0.
    SMRiQ9JMK0. Positions 114-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 388353LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni169 – 1735UDP-alpha-D-galactose bindingBy similarity
    Regioni208 – 2103UDP-alpha-D-galactose bindingBy similarity
    Regioni235 – 2362UDP-alpha-D-galactose bindingBy similarity
    Regioni298 – 3014N-acetyl-D-glucosamine bindingBy similarity
    Regioni329 – 3302UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00750000117412.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiA2A5T7.
    KOiK09905.
    OMAiLPYTEFF.
    OrthoDBiEOG7060R0.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9JMK0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG    50
    ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT 100
    FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG 150
    GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI 200
    EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ 250
    MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED 300
    DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER 350
    QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY 388
    Length:388
    Mass (Da):44,814
    Last modified:July 27, 2011 - v2
    Checksum:iFB3D6FBA2F8B3775
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti192 – 1921R → A in BAA94791. (PubMed:11145975)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004786 mRNA. Translation: BAA94791.1.
    AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
    CH466551 Genomic DNA. Translation: EDL06506.1.
    CCDSiCCDS17098.1.
    RefSeqiNP_062809.2. NM_019835.2.
    UniGeneiMm.200886.
    Mm.489868.

    Genome annotation databases

    EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
    GeneIDi56336.
    KEGGimmu:56336.
    UCSCiuc008nzm.1. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT5

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB004786 mRNA. Translation: BAA94791.1 .
    AL591854 , AL591762 Genomic DNA. Translation: CAM13428.1 .
    CH466551 Genomic DNA. Translation: EDL06506.1 .
    CCDSi CCDS17098.1.
    RefSeqi NP_062809.2. NM_019835.2.
    UniGenei Mm.200886.
    Mm.489868.

    3D structure databases

    ProteinModelPortali Q9JMK0.
    SMRi Q9JMK0. Positions 114-380.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q9JMK0.

    Proteomic databases

    PRIDEi Q9JMK0.

    Protocols and materials databases

    DNASUi 56336.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000109221 ; ENSMUSP00000104844 ; ENSMUSG00000017929 .
    GeneIDi 56336.
    KEGGi mmu:56336.
    UCSCi uc008nzm.1. mouse.

    Organism-specific databases

    CTDi 9334.
    MGIi MGI:1927169. B4galt5.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00750000117412.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi A2A5T7.
    KOi K09905.
    OMAi LPYTEFF.
    OrthoDBi EOG7060R0.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198517. O-linked glycosylation of mucins.
    REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    ChiTaRSi B4GALT5. mouse.
    NextBioi 312326.
    PROi Q9JMK0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JMK0.
    Bgeei Q9JMK0.
    Genevestigatori Q9JMK0.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
      Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
      J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Brain.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiB4GT5_MOUSE
    AccessioniPrimary (citable) accession number: Q9JMK0
    Secondary accession number(s): A2A5T7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 107 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3