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Q9JMK0

- B4GT5_MOUSE

UniProt

Q9JMK0 - B4GT5_MOUSE

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Protein
Beta-1,4-galactosyltransferase 5
Gene
B4galt5, Bgt-5
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids By similarity.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi236 – 2361Manganese By similarity
Binding sitei264 – 2641UDP-alpha-D-galactose By similarity
Binding sitei296 – 2961UDP-alpha-D-galactose By similarity
Metal bindingi329 – 3291Manganese; via tele nitrogen By similarity
Binding sitei340 – 3401N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198517. O-linked glycosylation of mucins.
REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 5 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 5
Short name:
Beta4Gal-T5
Short name:
b4Gal-T5
Alternative name(s):
Beta-1,4-GalT II
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
Gene namesi
Name:B4galt5
Synonyms:Bgt-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:1927169. B4galt5.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei15 – 3521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini36 – 388353Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388Beta-1,4-galactosyltransferase 5
PRO_0000080546Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi77 – 771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi81 – 811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi90 – 901N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi114 ↔ 158 By similarity
Glycosylationi128 – 1281N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi229 ↔ 248 By similarity
Glycosylationi360 – 3601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi364 – 3641N-linked (GlcNAc...) Reviewed prediction
Glycosylationi373 – 3731N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9JMK0.

PTM databases

PhosphoSiteiQ9JMK0.

Expressioni

Tissue specificityi

Highest levels in heart, brain, liver and kidney with lower levels in spleen, lung and testis.1 Publication

Developmental stagei

In the brain, expression increases after birth.1 Publication

Gene expression databases

ArrayExpressiQ9JMK0.
BgeeiQ9JMK0.
GenevestigatoriQ9JMK0.

Structurei

3D structure databases

ProteinModelPortaliQ9JMK0.
SMRiQ9JMK0. Positions 114-380.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni169 – 1735UDP-alpha-D-galactose binding By similarity
Regioni208 – 2103UDP-alpha-D-galactose binding By similarity
Regioni235 – 2362UDP-alpha-D-galactose binding By similarity
Regioni298 – 3014N-acetyl-D-glucosamine binding By similarity
Regioni329 – 3302UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00750000117412.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiA2A5T7.
KOiK09905.
OMAiLPYTEFF.
OrthoDBiEOG7060R0.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JMK0-1 [UniParc]FASTAAdd to Basket

« Hide

MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG    50
ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT 100
FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG 150
GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI 200
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ 250
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED 300
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER 350
QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY 388
Length:388
Mass (Da):44,814
Last modified:July 27, 2011 - v2
Checksum:iFB3D6FBA2F8B3775
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti192 – 1921R → A in BAA94791. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004786 mRNA. Translation: BAA94791.1.
AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
CH466551 Genomic DNA. Translation: EDL06506.1.
CCDSiCCDS17098.1.
RefSeqiNP_062809.2. NM_019835.2.
UniGeneiMm.200886.
Mm.489868.

Genome annotation databases

EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
GeneIDi56336.
KEGGimmu:56336.
UCSCiuc008nzm.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT5

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB004786 mRNA. Translation: BAA94791.1 .
AL591854 , AL591762 Genomic DNA. Translation: CAM13428.1 .
CH466551 Genomic DNA. Translation: EDL06506.1 .
CCDSi CCDS17098.1.
RefSeqi NP_062809.2. NM_019835.2.
UniGenei Mm.200886.
Mm.489868.

3D structure databases

ProteinModelPortali Q9JMK0.
SMRi Q9JMK0. Positions 114-380.
ModBasei Search...

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei Q9JMK0.

Proteomic databases

PRIDEi Q9JMK0.

Protocols and materials databases

DNASUi 56336.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000109221 ; ENSMUSP00000104844 ; ENSMUSG00000017929 .
GeneIDi 56336.
KEGGi mmu:56336.
UCSCi uc008nzm.1. mouse.

Organism-specific databases

CTDi 9334.
MGIi MGI:1927169. B4galt5.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00750000117412.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi A2A5T7.
KOi K09905.
OMAi LPYTEFF.
OrthoDBi EOG7060R0.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198517. O-linked glycosylation of mucins.
REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSi B4GALT5. mouse.
NextBioi 312326.
PROi Q9JMK0.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9JMK0.
Bgeei Q9JMK0.
Genevestigatori Q9JMK0.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Differential gene expression of beta-1,4-galactosyltransferases I, II and V during mouse brain development."
    Nakamura N., Yamakawa N., Sato T., Tojo H., Tachi C., Furukawa K.
    J. Neurochem. 76:29-38(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: BALB/c.
    Tissue: Brain.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiB4GT5_MOUSE
AccessioniPrimary (citable) accession number: Q9JMK0
Secondary accession number(s): A2A5T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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