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Protein

Beta-1,4-galactosyltransferase 5

Gene

B4galt5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.By similarity

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi236ManganeseBy similarity1
Binding sitei264UDP-alpha-D-galactoseBy similarity1
Binding sitei296UDP-alpha-D-galactoseBy similarity1
Metal bindingi329Manganese; via tele nitrogenBy similarity1
Binding sitei340N-acetyl-D-glucosamineBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.
R-MMU-975577. N-Glycan antennae elongation.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Chemistry databases

SwissLipidsiSLP:000000786.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 5 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 5
Short name:
Beta4Gal-T5
Short name:
b4Gal-T5
Alternative name(s):
Beta-1,4-GalT II
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 5
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 5
Gene namesi
Name:B4galt5
Synonyms:Bgt-5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1927169. B4galt5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicSequence analysisAdd BLAST14
Transmembranei15 – 35Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini36 – 388LumenalSequence analysisAdd BLAST353

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000805461 – 388Beta-1,4-galactosyltransferase 5Add BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi90N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi114 ↔ 158By similarity
Glycosylationi128N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi229 ↔ 248By similarity
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Glycosylationi364N-linked (GlcNAc...)Sequence analysis1
Glycosylationi373N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiQ9JMK0.
MaxQBiQ9JMK0.
PaxDbiQ9JMK0.
PRIDEiQ9JMK0.

PTM databases

PhosphoSitePlusiQ9JMK0.

Expressioni

Tissue specificityi

Highest levels in heart, brain, liver and kidney with lower levels in spleen, lung and testis.1 Publication

Developmental stagei

In the brain, expression increases after birth.1 Publication

Gene expression databases

BgeeiENSMUSG00000017929.
ExpressionAtlasiQ9JMK0. baseline and differential.
GenevisibleiQ9JMK0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000104844.

Structurei

3D structure databases

ProteinModelPortaliQ9JMK0.
SMRiQ9JMK0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni169 – 173UDP-alpha-D-galactose bindingBy similarity5
Regioni208 – 210UDP-alpha-D-galactose bindingBy similarity3
Regioni235 – 236UDP-alpha-D-galactose bindingBy similarity2
Regioni298 – 301N-acetyl-D-glucosamine bindingBy similarity4
Regioni329 – 330UDP-alpha-D-galactose bindingBy similarity2

Sequence similaritiesi

Belongs to the glycosyltransferase 7 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9JMK0.
KOiK09905.
OMAiVPRWKVA.
OrthoDBiEOG091G0P66.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JMK0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRARRGLLRL PRRSLLAALF FFSLSSSLLY FVYVAPGIVN TYLFMVQAQG
60 70 80 90 100
ILLRDNVRTI GAQVYEQVVR SAYAKRNSSL NDSDYPLDLN HSEAFPPTTT
110 120 130 140 150
FLPEDFTYFA NHPCPERLPS MKGPIDINMS EIAMDDIHEL FSRDPAIKLG
160 170 180 190 200
GHWKPADCVP RWKVAILIPF RNRHEHLPVL LRHLLPMLQR QRLQFAFYVI
210 220 230 240 250
EQVGTQPFNR AMLFNVGFQE AMKDLDWDCL IFHDVDHIPE SDRNYYGCGQ
260 270 280 290 300
MPRHFATKLD KYMYLLPYTE FFGGVSGLTV EQFRKINGFP NAFWGWGGED
310 320 330 340 350
DDLWNRVQNA GYSVSRPEGD TGKYKSIPHH HRGEVQFLGR YALLRKSKER
360 370 380
QGLDGLNNLN YSANVTYDAL YKNITVNLTP ELAQVTEY
Length:388
Mass (Da):44,814
Last modified:July 27, 2011 - v2
Checksum:iFB3D6FBA2F8B3775
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti192R → A in BAA94791 (PubMed:11145975).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004786 mRNA. Translation: BAA94791.1.
AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
CH466551 Genomic DNA. Translation: EDL06506.1.
CCDSiCCDS17098.1.
RefSeqiNP_062809.2. NM_019835.2.
UniGeneiMm.200886.
Mm.489868.

Genome annotation databases

EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
GeneIDi56336.
KEGGimmu:56336.
UCSCiuc008nzm.1. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB004786 mRNA. Translation: BAA94791.1.
AL591854, AL591762 Genomic DNA. Translation: CAM13428.1.
CH466551 Genomic DNA. Translation: EDL06506.1.
CCDSiCCDS17098.1.
RefSeqiNP_062809.2. NM_019835.2.
UniGeneiMm.200886.
Mm.489868.

3D structure databases

ProteinModelPortaliQ9JMK0.
SMRiQ9JMK0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000104844.

Chemistry databases

SwissLipidsiSLP:000000786.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitePlusiQ9JMK0.

Proteomic databases

EPDiQ9JMK0.
MaxQBiQ9JMK0.
PaxDbiQ9JMK0.
PRIDEiQ9JMK0.

Protocols and materials databases

DNASUi56336.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000109221; ENSMUSP00000104844; ENSMUSG00000017929.
GeneIDi56336.
KEGGimmu:56336.
UCSCiuc008nzm.1. mouse.

Organism-specific databases

CTDi9334.
MGIiMGI:1927169. B4galt5.

Phylogenomic databases

eggNOGiKOG3916. Eukaryota.
ENOG410ZYYA. LUCA.
GeneTreeiENSGT00760000119140.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9JMK0.
KOiK09905.
OMAiVPRWKVA.
OrthoDBiEOG091G0P66.
TreeFamiTF312834.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
R-MMU-913709. O-linked glycosylation of mucins.
R-MMU-975577. N-Glycan antennae elongation.

Miscellaneous databases

ChiTaRSiB4galt5. mouse.
PROiQ9JMK0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017929.
ExpressionAtlasiQ9JMK0. baseline and differential.
GenevisibleiQ9JMK0. MM.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB4GT5_MOUSE
AccessioniPrimary (citable) accession number: Q9JMK0
Secondary accession number(s): A2A5T7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.