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Q9JMI9 (TRPC3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Short transient receptor potential channel 3

Short name=TrpC3
Alternative name(s):
Trp-related protein 3
Gene names
Name:Trpc3
Synonyms:Trrp3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length836 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol 1,4,5-triphosphate receptors (ITPR) with bound IP3 By similarity. May also be activated by internal calcium store depletion.

Subunit structure

Interacts with TRPC1, ITPR1, ITPR3, MX1 and RNF24. Interacts with JPH2; the interaction is involved in maintaining Ca2+ homeostasis in skeletal muscle and is mediated by JPH2 'Ser-165' phosphorylation By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Probable.

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC3 sub-subfamily. [View classification]

Contains 4 ANK repeats.

Sequence caution

The sequence BAA93434.1 differs from that shown. Reason: Chimeric cDNA.

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainANK repeat
Repeat
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionCalcium channel
Ion channel
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncalcium activated cation channel activity

Inferred from direct assay Ref.2. Source: RGD

calcium channel activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Select]
Isoform 1 (identifier: Q9JMI9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9JMI9-2)

Also known as: Trp3SV;

The sequence of this isoform is not available.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 836836Short transient receptor potential channel 3
PRO_0000215312

Regions

Topological domain1 – 369369Cytoplasmic Potential
Transmembrane370 – 39021Helical; Potential
Topological domain391 – 41828Extracellular Potential
Transmembrane419 – 43921Helical; Potential
Topological domain440 – 45112Cytoplasmic Potential
Transmembrane452 – 47221Helical; Potential
Topological domain473 – 52351Extracellular Potential
Transmembrane524 – 54421Helical; Potential
Topological domain545 – 56723Cytoplasmic Potential
Transmembrane568 – 58821Helical; Potential
Topological domain589 – 63749Extracellular Potential
Transmembrane638 – 65821Helical; Potential
Topological domain659 – 836178Cytoplasmic Potential
Repeat26 – 5530ANK 1
Repeat61 – 9030ANK 2
Repeat92 – 11827ANK 3
Repeat147 – 17630ANK 4

Amino acid modifications

Glycosylation4041N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3351A → S in BAA93434. Ref.2
Sequence conflict3611S → T in BAA93434. Ref.2
Sequence conflict3941D → H in BAA93434. Ref.2
Sequence conflict4861Q → H in BAA93434. Ref.2
Sequence conflict5061E → K in BAA93434. Ref.2
Sequence conflict5631K → E in BAA93434. Ref.2
Sequence conflict5671K → Q in BAA93434. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 26, 2009. Version 3.
Checksum: E405FBFC9285613F

FASTA83695,682
        10         20         30         40         50         60 
MRDKGRRQAV RGPAFMFGAR GPSLTAEEER FLDAAEYGNI PVVRKMLEES RTLNVNCVDY 

        70         80         90        100        110        120 
MGQNALQLAV GNEHLEVTEL LLKKENLARI GDALLLAISK GYVRIVEAIL GHPGFAASRR 

       130        140        150        160        170        180 
LTLSPCEQEL RDDDFYAYDE DGTRFSPDIT PIILAAHCHK YEVVHLLLLK GARIERPHDY 

       190        200        210        220        230        240 
FCRCADCAEK QRLDAFSHSR SRINAYKGLA SPAYLSLSSE DPVLTALELS NELAKLANIE 

       250        260        270        280        290        300 
KEFKNDYRKL SMQCKDFVVG VLDLCRDSEE VEAILNGDLE SVEPLERHGH KASLSRVKLA 

       310        320        330        340        350        360 
IKYEVKKFVA HPNCQQQLLT IWYENLSGLR EQTIAIKCLV VLVVALGLPF LAIGYWIAPC 

       370        380        390        400        410        420 
SRLGKILRSP FMKFVAHAAS FIIFLGLLVF NASDRFEGIT TLPNITVIDY PKQIFRVKTT 

       430        440        450        460        470        480 
QFTWTEMLIM VWVLGMMWSE CKELWLEGPR EYIVQLWNVL DFGMLSIFIA AFTARFLAFL 

       490        500        510        520        530        540 
QATKAQQYVD SHVQESDLSE VTLPPEVQYF TYARDKWLPS DPQIISEGLY AIAVVLSFSR 

       550        560        570        580        590        600 
IAYILPANES FGPLQISLGR TVKDIFKFMV LFIMVFLAFM IGMFILYSYY LGAKVNPAFT 

       610        620        630        640        650        660 
TVEESFKTLF WSIFGLSEVT SVVLKYDHKF IENIGYVLYG IYNVTMVVVL LNMLIAMINS 

       670        680        690        700        710        720 
SYQEIEDDSD VEWKFARSKL WLSYFDDGKT LPPPFSLVPS PKSFVYFIMR ITNFSKCRRR 

       730        740        750        760        770        780 
RLQKDLELGM GNSKSRLNLF TQSNSRVFES HSFNSILNQP TRYQQIMKRL IKRYVLKAQV 

       790        800        810        820        830 
DKENDEVNEG ELKEIKQDIS SLRYELLEDK SQATEELAIL IHKLSEKLNP SALRCE 

« Hide

Isoform 2 (Trp3SV) (Sequence not available).

References

« Hide 'large scale' references
[1]"Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. expand/collapse author list , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[2]"A calcium-activated cation current by an alternatively spliced form of Trp3 in the heart."
Ohki G., Miyoshi T., Murata M., Ishibashi K., Imai M., Suzuki M.
J. Biol. Chem. 275:39055-39060(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 133-836.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AABR03013307 Genomic DNA. No translation available.
AABR03015957 Genomic DNA. No translation available.
AB022331 mRNA. Translation: BAA93434.1. Sequence problems.
UniGeneRn.45385.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-59688N.

PTM databases

PhosphoSiteQ9JMI9.

Proteomic databases

PRIDEQ9JMI9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:61973. rat. [Q9JMI9-1]

Organism-specific databases

RGD61973. Trpc3.

Phylogenomic databases

eggNOGNOG254238.
HOGENOMHOG000020590.
HOVERGENHBG068337.
PhylomeDBQ9JMI9.

Gene expression databases

GenevestigatorQ9JMI9.

Family and domain databases

Gene3D1.25.40.20. 1 hit.
InterProIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR005821. Ion_trans_dom.
IPR004729. TRP_channel.
IPR013555. TRP_dom.
IPR005459. TRPC3_channel.
IPR002153. TRPC_channel.
[Graphical view]
PANTHERPTHR10117. PTHR10117. 1 hit.
PTHR10117:SF8. PTHR10117:SF8. 1 hit.
PfamPF12796. Ank_2. 1 hit.
PF00520. Ion_trans. 1 hit.
PF08344. TRP_2. 1 hit.
[Graphical view]
PRINTSPR01097. TRNSRECEPTRP.
PR01644. TRPCHANNEL3.
SMARTSM00248. ANK. 3 hits.
[Graphical view]
SUPFAMSSF48403. SSF48403. 1 hit.
TIGRFAMsTIGR00870. trp. 1 hit.
PROSITEPS50297. ANK_REP_REGION. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROQ9JMI9.

Entry information

Entry nameTRPC3_RAT
AccessionPrimary (citable) accession number: Q9JMI9
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 26, 2009
Last modified: April 16, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families