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Protein

Acetoacetyl-CoA synthetase

Gene

Aacs

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.

Catalytic activityi

ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.1 Publication

GO - Molecular functioni

  • acetoacetate-CoA ligase activity Source: RGD
  • ATP binding Source: UniProtKB-KW
  • butyrate-CoA ligase activity Source: RGD

GO - Biological processi

  • adipose tissue development Source: RGD
  • cellular response to cholesterol Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to testosterone stimulus Source: RGD
  • fatty acid metabolic process Source: UniProtKB-KW
  • liver development Source: RGD
  • positive regulation of insulin secretion Source: RGD
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to nutrient Source: RGD
  • response to oleic acid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to purine-containing compound Source: RGD
  • response to starvation Source: RGD
  • white fat cell differentiation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.16. 5301.
ReactomeiR-RNO-77111. Synthesis of Ketone Bodies.
SABIO-RKQ9JMI1.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetoacetyl-CoA synthetase (EC:6.2.1.16)
Gene namesi
Name:Aacs
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi708522. Aacs.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 672672Acetoacetyl-CoA synthetasePRO_0000315787Add
BLAST

Proteomic databases

PaxDbiQ9JMI1.
PRIDEiQ9JMI1.

PTM databases

iPTMnetiQ9JMI1.
PhosphoSiteiQ9JMI1.

Expressioni

Tissue specificityi

Abundant in male subcutaneous white adipose tissue after weaning. In white adipose tissue, it is preferentially detected in mature adipocytes but not in preadipocytes. The expression in primary preadipocytes increases during the adipocyte differentiation. In brain, it is expressed in the midbrain, pons/medulla, cerebral cortex, hippocampus and cerebellum. The expression in the cerebellum is restricted primarily to glial cells, while in the cerebral cortex, it is restricted to neuronal cells.2 Publications

Gene expression databases

BgeeiENSRNOG00000000967.
GenevisibleiQ9JMI1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001292.

Structurei

3D structure databases

ProteinModelPortaliQ9JMI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00820000127056.
HOGENOMiHOG000229992.
HOVERGENiHBG059947.
InParanoidiQ9JMI1.
KOiK01907.
OMAiVWDFCGI.
OrthoDBiEOG091G05B0.
PhylomeDBiQ9JMI1.
TreeFamiTF354241.

Family and domain databases

InterProiIPR005914. Acac_CoA_synth.
IPR032387. ACAS_N.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JMI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLARLERE EIMECQVMWE PDSKKDTQMD RFRAAVGTAC GLALGNYDDL
60 70 80 90 100
YHWSVRSYSD FWAEFWKFSG IVCSRMYDEV VDTSKGIADV PEWFRGSRLN
110 120 130 140 150
YAENLLRHKE NDRVALYVAR EGREEIAKVT FEELRQQVAL FAAAMRKMGV
160 170 180 190 200
KKGDRVVGYL PNSAHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ
210 220 230 240 250
PKLIFSVEAV VYNGKEHGHL EKLQRVVKGL PDLQRVVLIP YVLPREKIDI
260 270 280 290 300
SKIPNSMFLD DFLASGTGAQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM
310 320 330 340 350
VHSAGGTLIQ HLKEHVLHGN MTSSDILLYY TTVGWMMWNW MVSALATGAS
360 370 380 390 400
LVLYDGSPLV PTPNVLWDLV DRIGITILGT GAKWLSVLEE KDMKPMETHN
410 420 430 440 450
LHTLHTILST GSPLKAQSYE YVYRCIKSTV LLGSISGGTD IISCFMGQNS
460 470 480 490 500
SIPVYKGEIQ ARNLGMAVEA WDEEGKTVWG ASGELVCTKP IPCQPTHFWN
510 520 530 540 550
DENGSKYRKA YFSKYPGVWA HGDYCRINPK TGGIVMLGRS DGTLNPNGVR
560 570 580 590 600
FGSSEIYNIV EAFDEVEDSL CVPQYNRDGE ERVVLFLKMA SGHTFQPDLV
610 620 630 640 650
KHIRDAIRLG LSARHVPSLI LETQGIPYTI NGKKVEVAVK QVIAGKTVEH
660 670
RGAFSNPESL DLYRDIPELQ DF
Length:672
Mass (Da):75,040
Last modified:October 1, 2000 - v1
Checksum:iFB6FD5C6D1A927A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026291 mRNA. Translation: BAA90828.1.
BC061803 mRNA. Translation: AAH61803.1.
RefSeqiNP_075592.1. NM_023104.1.
UniGeneiRn.9215.

Genome annotation databases

EnsembliENSRNOT00000001292; ENSRNOP00000001292; ENSRNOG00000000967.
GeneIDi65984.
KEGGirno:65984.
UCSCiRGD:708522. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026291 mRNA. Translation: BAA90828.1.
BC061803 mRNA. Translation: AAH61803.1.
RefSeqiNP_075592.1. NM_023104.1.
UniGeneiRn.9215.

3D structure databases

ProteinModelPortaliQ9JMI1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001292.

PTM databases

iPTMnetiQ9JMI1.
PhosphoSiteiQ9JMI1.

Proteomic databases

PaxDbiQ9JMI1.
PRIDEiQ9JMI1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001292; ENSRNOP00000001292; ENSRNOG00000000967.
GeneIDi65984.
KEGGirno:65984.
UCSCiRGD:708522. rat.

Organism-specific databases

CTDi65985.
RGDi708522. Aacs.

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00820000127056.
HOGENOMiHOG000229992.
HOVERGENiHBG059947.
InParanoidiQ9JMI1.
KOiK01907.
OMAiVWDFCGI.
OrthoDBiEOG091G05B0.
PhylomeDBiQ9JMI1.
TreeFamiTF354241.

Enzyme and pathway databases

BRENDAi6.2.1.16. 5301.
ReactomeiR-RNO-77111. Synthesis of Ketone Bodies.
SABIO-RKQ9JMI1.

Miscellaneous databases

PROiQ9JMI1.

Gene expression databases

BgeeiENSRNOG00000000967.
GenevisibleiQ9JMI1. RN.

Family and domain databases

InterProiIPR005914. Acac_CoA_synth.
IPR032387. ACAS_N.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAACS_RAT
AccessioniPrimary (citable) accession number: Q9JMI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.