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Protein

Acetoacetyl-CoA synthetase

Gene

Aacs

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development.

Catalytic activityi

ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.1 Publication

GO - Molecular functioni

  • acetoacetate-CoA ligase activity Source: RGD
  • ATP binding Source: UniProtKB-KW
  • butyrate-CoA ligase activity Source: RGD

GO - Biological processi

  • adipose tissue development Source: RGD
  • cellular response to cholesterol Source: RGD
  • cellular response to glucose stimulus Source: RGD
  • cellular response to testosterone stimulus Source: RGD
  • fatty acid metabolic process Source: UniProtKB-KW
  • liver development Source: RGD
  • positive regulation of insulin secretion Source: RGD
  • response to drug Source: RGD
  • response to ethanol Source: RGD
  • response to nutrient Source: RGD
  • response to oleic acid Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organonitrogen compound Source: RGD
  • response to purine-containing compound Source: RGD
  • response to starvation Source: RGD
  • white fat cell differentiation Source: RGD

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.16. 5301.
ReactomeiR-RNO-77111. Synthesis of Ketone Bodies.
SABIO-RKiQ9JMI1.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetoacetyl-CoA synthetase (EC:6.2.1.16)
Gene namesi
Name:Aacs
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi708522. Aacs.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003157871 – 672Acetoacetyl-CoA synthetaseAdd BLAST672

Proteomic databases

PaxDbiQ9JMI1.
PRIDEiQ9JMI1.

PTM databases

iPTMnetiQ9JMI1.
PhosphoSitePlusiQ9JMI1.

Expressioni

Tissue specificityi

Abundant in male subcutaneous white adipose tissue after weaning. In white adipose tissue, it is preferentially detected in mature adipocytes but not in preadipocytes. The expression in primary preadipocytes increases during the adipocyte differentiation. In brain, it is expressed in the midbrain, pons/medulla, cerebral cortex, hippocampus and cerebellum. The expression in the cerebellum is restricted primarily to glial cells, while in the cerebral cortex, it is restricted to neuronal cells.2 Publications

Gene expression databases

BgeeiENSRNOG00000000967.
GenevisibleiQ9JMI1. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001292.

Structurei

3D structure databases

ProteinModelPortaliQ9JMI1.
SMRiQ9JMI1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1175. Eukaryota.
COG0365. LUCA.
GeneTreeiENSGT00900000141060.
HOGENOMiHOG000229992.
HOVERGENiHBG059947.
InParanoidiQ9JMI1.
KOiK01907.
OMAiYEYVYRC.
OrthoDBiEOG091G05B0.
PhylomeDBiQ9JMI1.
TreeFamiTF354241.

Family and domain databases

InterProiView protein in InterPro
IPR005914. Acac_CoA_synth.
IPR032387. ACAS_N.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
PfamiView protein in Pfam
PF16177. ACAS_N. 1 hit.
PF00501. AMP-binding. 1 hit.
TIGRFAMsiTIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JMI1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKLARLERE EIMECQVMWE PDSKKDTQMD RFRAAVGTAC GLALGNYDDL
60 70 80 90 100
YHWSVRSYSD FWAEFWKFSG IVCSRMYDEV VDTSKGIADV PEWFRGSRLN
110 120 130 140 150
YAENLLRHKE NDRVALYVAR EGREEIAKVT FEELRQQVAL FAAAMRKMGV
160 170 180 190 200
KKGDRVVGYL PNSAHAVEAM LAAASIGAIW SSTSPDFGVN GVLDRFSQIQ
210 220 230 240 250
PKLIFSVEAV VYNGKEHGHL EKLQRVVKGL PDLQRVVLIP YVLPREKIDI
260 270 280 290 300
SKIPNSMFLD DFLASGTGAQ APQLEFEQLP FSHPLFIMFS SGTTGAPKCM
310 320 330 340 350
VHSAGGTLIQ HLKEHVLHGN MTSSDILLYY TTVGWMMWNW MVSALATGAS
360 370 380 390 400
LVLYDGSPLV PTPNVLWDLV DRIGITILGT GAKWLSVLEE KDMKPMETHN
410 420 430 440 450
LHTLHTILST GSPLKAQSYE YVYRCIKSTV LLGSISGGTD IISCFMGQNS
460 470 480 490 500
SIPVYKGEIQ ARNLGMAVEA WDEEGKTVWG ASGELVCTKP IPCQPTHFWN
510 520 530 540 550
DENGSKYRKA YFSKYPGVWA HGDYCRINPK TGGIVMLGRS DGTLNPNGVR
560 570 580 590 600
FGSSEIYNIV EAFDEVEDSL CVPQYNRDGE ERVVLFLKMA SGHTFQPDLV
610 620 630 640 650
KHIRDAIRLG LSARHVPSLI LETQGIPYTI NGKKVEVAVK QVIAGKTVEH
660 670
RGAFSNPESL DLYRDIPELQ DF
Length:672
Mass (Da):75,040
Last modified:October 1, 2000 - v1
Checksum:iFB6FD5C6D1A927A9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026291 mRNA. Translation: BAA90828.1.
BC061803 mRNA. Translation: AAH61803.1.
RefSeqiNP_075592.1. NM_023104.1.
UniGeneiRn.9215.

Genome annotation databases

EnsembliENSRNOT00000001292; ENSRNOP00000001292; ENSRNOG00000000967.
GeneIDi65984.
KEGGirno:65984.
UCSCiRGD:708522. rat.

Similar proteinsi

Entry informationi

Entry nameiAACS_RAT
AccessioniPrimary (citable) accession number: Q9JMI1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: October 25, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families