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Protein

Endothelin-converting enzyme-like 1

Gene

Ecel1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

May contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi612Zinc; catalyticPROSITE-ProRule annotation1
Active sitei613PROSITE-ProRule annotation1
Metal bindingi616Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi672Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • metalloendopeptidase activity Source: MGI

GO - Biological processi

  • neuropeptide signaling pathway Source: InterPro
  • respiratory system process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM13.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Endothelin-converting enzyme-like 1 (EC:3.4.24.-)
Alternative name(s):
Damage-induced neuronal endopeptidase
Xce protein
Gene namesi
Name:Ecel1
Synonyms:Dine, Xce
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1343461. Ecel1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 61CytoplasmicSequence analysisAdd BLAST61
Transmembranei62 – 82Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini83 – 775LumenalSequence analysisAdd BLAST693

GO - Cellular componenti

  • integral component of plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

According to a report, mice die of respiratory failure shortly after birth (PubMed:10400672). According to a second report, mice exhibit perturbed terminal branching of motor neurons to the endplate of skeletal muscles, resulting in poor formation of the neuromuscular junction (PubMed:23261301).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000782251 – 775Endothelin-converting enzyme-like 1Add BLAST775

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1
Glycosylationi322N-linked (GlcNAc...)Sequence analysis1
Glycosylationi656N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9JMI0.
PaxDbiQ9JMI0.
PRIDEiQ9JMI0.

PTM databases

iPTMnetiQ9JMI0.
PhosphoSitePlusiQ9JMI0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026247.
CleanExiMM_ECEL1.
GenevisibleiQ9JMI0. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9JMI0. 1 interactor.
STRINGi10090.ENSMUSP00000027463.

Structurei

3D structure databases

ProteinModelPortaliQ9JMI0.
SMRiQ9JMI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M13 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiQ9JMI0.
KOiK09610.
OMAiHLYDNFT.
OrthoDBiEOG091G025Y.
PhylomeDBiQ9JMI0.
TreeFamiTF315192.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR029736. ECEL1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF108. PTHR11733:SF108. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JMI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEAPYSMTAH YDEFQEVKYV SRCGTGGARG TSLPPGFPRG SGRSASGSRS
60 70 80 90 100
GLPRWNRREV CLLSGLVFAA GLCAILAAML ALKYLGPGAA GGGGACPEGC
110 120 130 140 150
PERKAFARAA RFLSANLDAS IDPCQDFYSF ACGGWLRRHA IPDDKLTYGT
160 170 180 190 200
IAAIGEQNEE RLRRLLARPT GGPGGAAQRK VRAFFRSCLD MREIERLGPR
210 220 230 240 250
PMLEVIEDCG GWDLGGAADR PGAARWDLNR LLYKAQGVYS AAALFSLTVS
260 270 280 290 300
LDDRNSSRYV IRIDQDGLTL PERTLYLAQD EESEKILAAY RVFMQRLLRL
310 320 330 340 350
LGADAVEQKA QEILQLEQRL ANISVSEYDD LRRDVSSAYN KVTLGQLQKI
360 370 380 390 400
IPHLQWKWLL DQIFQEDFSE EEEVVLLATD YMQQVSQLIR STPRRILHNY
410 420 430 440 450
LVWRVVVVLS EHLSSPFREA LHELAKEMEG NDKPQELARV CLGQANRHFG
460 470 480 490 500
MALGALFVHE HFSAASKAKV QQLVEDIKYI LGQRLEELDW MDAQTKAAAR
510 520 530 540 550
AKLQYMMVMV GYPDFLLKPE AVDKEYEFEV HEKTYFKNIL NSIRFSIQLS
560 570 580 590 600
VKKIRQEVDK SSWLLPPQAL NAYYLPNKNQ MVFPAGILQP TLYDPDFPQS
610 620 630 640 650
LNYGGIGTII GHELTHGYDD WGGQYDRSGN LLHWWTETSY SHFLRKAECI
660 670 680 690 700
VRLYDNFTVY NQRVNGKHTL GENIADMGGL KLAYYAYQKW VREHGPEHPL
710 720 730 740 750
HRLKYTHNQL FFIAFAQNWC IKRRSQSIYL QVLTDKHAPE HYRVLGSVSQ
760 770
FEEFGRAFHC PKDSPMNPVH KCSVW
Length:775
Mass (Da):87,993
Last modified:July 27, 2011 - v2
Checksum:i37ACB98A53E5B48B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti562S → T in BAA95005 (PubMed:10759559).Curated1
Sequence conflicti583F → Y in BAA95005 (PubMed:10759559).Curated1
Sequence conflicti638T → A in BAA95005 (PubMed:10759559).Curated1
Sequence conflicti642H → R in BAA95005 (PubMed:10759559).Curated1
Sequence conflicti645R → H in BAA95005 (PubMed:10759559).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026294 mRNA. Translation: BAA95005.1.
CH466520 Genomic DNA. Translation: EDL40189.1.
BC057569 mRNA. Translation: AAH57569.1.
CCDSiCCDS15128.1.
RefSeqiNP_001264854.1. NM_001277925.1.
NP_067281.2. NM_021306.3.
XP_017170387.1. XM_017314898.1.
XP_017170391.1. XM_017314902.1.
XP_017170392.1. XM_017314903.1.
UniGeneiMm.140765.

Genome annotation databases

EnsembliENSMUST00000027463; ENSMUSP00000027463; ENSMUSG00000026247.
ENSMUST00000160810; ENSMUSP00000125557; ENSMUSG00000026247.
ENSMUST00000161002; ENSMUSP00000125096; ENSMUSG00000026247.
GeneIDi13599.
KEGGimmu:13599.
UCSCiuc007bwc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026294 mRNA. Translation: BAA95005.1.
CH466520 Genomic DNA. Translation: EDL40189.1.
BC057569 mRNA. Translation: AAH57569.1.
CCDSiCCDS15128.1.
RefSeqiNP_001264854.1. NM_001277925.1.
NP_067281.2. NM_021306.3.
XP_017170387.1. XM_017314898.1.
XP_017170391.1. XM_017314902.1.
XP_017170392.1. XM_017314903.1.
UniGeneiMm.140765.

3D structure databases

ProteinModelPortaliQ9JMI0.
SMRiQ9JMI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JMI0. 1 interactor.
STRINGi10090.ENSMUSP00000027463.

Protein family/group databases

MEROPSiM13.007.

PTM databases

iPTMnetiQ9JMI0.
PhosphoSitePlusiQ9JMI0.

Proteomic databases

MaxQBiQ9JMI0.
PaxDbiQ9JMI0.
PRIDEiQ9JMI0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027463; ENSMUSP00000027463; ENSMUSG00000026247.
ENSMUST00000160810; ENSMUSP00000125557; ENSMUSG00000026247.
ENSMUST00000161002; ENSMUSP00000125096; ENSMUSG00000026247.
GeneIDi13599.
KEGGimmu:13599.
UCSCiuc007bwc.2. mouse.

Organism-specific databases

CTDi9427.
MGIiMGI:1343461. Ecel1.

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
HOVERGENiHBG005554.
InParanoidiQ9JMI0.
KOiK09610.
OMAiHLYDNFT.
OrthoDBiEOG091G025Y.
PhylomeDBiQ9JMI0.
TreeFamiTF315192.

Miscellaneous databases

PROiQ9JMI0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026247.
CleanExiMM_ECEL1.
GenevisibleiQ9JMI0. MM.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR029736. ECEL1.
IPR024079. MetalloPept_cat_dom.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF108. PTHR11733:SF108. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECEL1_MOUSE
AccessioniPrimary (citable) accession number: Q9JMI0
Secondary accession number(s): Q6PFG4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.