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Protein

Sialidase-3

Gene

Neu3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates.1 Publication

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei25 – 251SubstrateBy similarity
Binding sitei45 – 451SubstrateBy similarity
Active sitei50 – 501Proton acceptorBy similarity
Binding sitei179 – 1791SubstrateBy similarity
Binding sitei181 – 1811SubstrateBy similarity
Binding sitei223 – 2231SubstrateBy similarity
Binding sitei243 – 2431SubstrateSequence Analysis
Binding sitei339 – 3391SubstrateBy similarity
Active sitei369 – 3691NucleophileBy similarity
Active sitei386 – 3861Sequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_310546. Sialic acid metabolism.
REACT_339900. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-3 (EC:3.2.1.18)
Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3
Gene namesi
Name:Neu3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1355305. Neu3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 418418Sialidase-3PRO_0000208904Add
BLAST

Proteomic databases

MaxQBiQ9JMH7.
PRIDEiQ9JMH7.

PTM databases

PhosphoSiteiQ9JMH7.

Expressioni

Tissue specificityi

Expressed in heart, brain and cerebral cortex.1 Publication

Gene expression databases

BgeeiQ9JMH7.
CleanExiMM_NEU3.
ExpressionAtlasiQ9JMH7. baseline and differential.
GenevisibleiQ9JMH7. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045222.

Structurei

3D structure databases

ProteinModelPortaliQ9JMH7.
SMRiQ9JMH7. Positions 10-410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati129 – 14012BNR 1Add
BLAST
Repeati203 – 21412BNR 2Add
BLAST
Repeati254 – 26512BNR 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi24 – 274FRIP motif

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 3 BNR repeats.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG84455.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ9JMH7.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG7MSMNP.
PhylomeDBiQ9JMH7.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.

Sequencei

Sequence statusi: Complete.

Q9JMH7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEVPPYSLS STLFQQEEQS GVTYRIPALL YLPPTHTFLA FAEKRTSVRD
60 70 80 90 100
EDAACLVLRR GLMKGRSVQW GPQRLLMEAT LPGHRTMNPC PVWEKNTGRV
110 120 130 140 150
YLFFICVRGH VTERCQIVWG KNAARLCFLC SEDAGCSWGE VKDLTEEVIG
160 170 180 190 200
SEVKRWATFA VGPGHGIQLH SGRLIIPAYA YYVSRWFLCF ACSVKPHSLM
210 220 230 240 250
IYSDDFGVTW HHGKFIEPQV TGECQVAEVA GTAGNPVLYC SARTPSRFRA
260 270 280 290 300
EAFSTDSGGC FQKPTLNPQL HEPRTGCQGS VVSFRPLKMP NTYQDSIGKG
310 320 330 340 350
APATQKCPLL DSPLEVEKGA ETPSATWLLY SHPTSKRKRI NLGIYYNRNP
360 370 380 390 400
LEVNCWSRPW ILNRGPSGYS DLAVVEEQDL VACLFECGEK NEYERIDFCL
410
FSDHEVLSCE DCTSPSSD
Length:418
Mass (Da):46,846
Last modified:October 1, 2000 - v1
Checksum:i64853FC963FE7686
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026842 mRNA. Translation: BAA92868.1.
CCDSiCCDS21489.1.
RefSeqiNP_057929.1. NM_016720.2.
XP_011240157.1. XM_011241855.1.
XP_011240158.1. XM_011241856.1.
XP_011240159.1. XM_011241857.1.
XP_011240160.1. XM_011241858.1.
UniGeneiMm.103703.

Genome annotation databases

EnsembliENSMUST00000036331; ENSMUSP00000045222; ENSMUSG00000035239.
GeneIDi50877.
KEGGimmu:50877.
UCSCiuc009imc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB026842 mRNA. Translation: BAA92868.1.
CCDSiCCDS21489.1.
RefSeqiNP_057929.1. NM_016720.2.
XP_011240157.1. XM_011241855.1.
XP_011240158.1. XM_011241856.1.
XP_011240159.1. XM_011241857.1.
XP_011240160.1. XM_011241858.1.
UniGeneiMm.103703.

3D structure databases

ProteinModelPortaliQ9JMH7.
SMRiQ9JMH7. Positions 10-410.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000045222.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSiteiQ9JMH7.

Proteomic databases

MaxQBiQ9JMH7.
PRIDEiQ9JMH7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036331; ENSMUSP00000045222; ENSMUSG00000035239.
GeneIDi50877.
KEGGimmu:50877.
UCSCiuc009imc.1. mouse.

Organism-specific databases

CTDi10825.
MGIiMGI:1355305. Neu3.

Phylogenomic databases

eggNOGiNOG84455.
GeneTreeiENSGT00390000011171.
HOGENOMiHOG000233778.
HOVERGENiHBG052608.
InParanoidiQ9JMH7.
KOiK12357.
OMAiVYLFFIC.
OrthoDBiEOG7MSMNP.
PhylomeDBiQ9JMH7.
TreeFamiTF331063.

Enzyme and pathway databases

ReactomeiREACT_310546. Sialic acid metabolism.
REACT_339900. Glycosphingolipid metabolism.

Miscellaneous databases

NextBioi307853.
PROiQ9JMH7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JMH7.
CleanExiMM_NEU3.
ExpressionAtlasiQ9JMH7. baseline and differential.
GenevisibleiQ9JMH7. MM.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026944. Sialidase-3.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAMiSSF50939. SSF50939. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation."
    Hasegawa T., Yamaguchi K., Wada T., Takeda A., Itoyama Y., Miyagi T.
    J. Biol. Chem. 275:8007-8015(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Brain.

Entry informationi

Entry nameiNEUR3_MOUSE
AccessioniPrimary (citable) accession number: Q9JMH7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: July 22, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.