Q9JMH7 (NEUR3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialidase-3
EC=3.2.1.18

Alternative name(s):
Ganglioside sialidase
Membrane sialidase
N-acetyl-alpha-neuraminidase 3

Gene names

Name:

Neu3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Plays a role in modulating the ganglioside content of the lipid bilayer at the level of membrane-bound sialyl glycoconjugates By similarity.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Membrane; Peripheral membrane protein By similarity.
Tissue specificity	Expressed in heart, brain and cerebral cortex.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 3 BNR repeats.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from sequence alignment PubMed 12730204. Source: MGI
Cellular_component	plasma membrane Inferred from sequence alignment PubMed 12730204. Source: MGI
Molecular_function	alpha-sialidase activity Inferred from sequence alignment PubMed 12730204. Source: MGI exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Sialidase-3		PRO_0000208904
Regions
	Repeat	129 – 140	12	BNR 1
	Repeat	203 – 214	12	BNR 2
	Repeat	254 – 265	12	BNR 3
	Motif	24 – 27	4	FRIP motif
Sites
	Active site	50	1	Proton acceptor By similarity
	Active site	369	1	Nucleophile By similarity
	Active site	386	1	Potential
	Binding site	25	1	Substrate By similarity
	Binding site	243	1	Substrate Potential
	Binding site	339	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JMH7 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 64853FC963FE7686	FASTA	418	46,846
	10 20 30 40 50 60 MEEVPPYSLS STLFQQEEQS GVTYRIPALL YLPPTHTFLA FAEKRTSVRD EDAACLVLRR 70 80 90 100 110 120 GLMKGRSVQW GPQRLLMEAT LPGHRTMNPC PVWEKNTGRV YLFFICVRGH VTERCQIVWG 130 140 150 160 170 180 KNAARLCFLC SEDAGCSWGE VKDLTEEVIG SEVKRWATFA VGPGHGIQLH SGRLIIPAYA 190 200 210 220 230 240 YYVSRWFLCF ACSVKPHSLM IYSDDFGVTW HHGKFIEPQV TGECQVAEVA GTAGNPVLYC 250 260 270 280 290 300 SARTPSRFRA EAFSTDSGGC FQKPTLNPQL HEPRTGCQGS VVSFRPLKMP NTYQDSIGKG 310 320 330 340 350 360 APATQKCPLL DSPLEVEKGA ETPSATWLLY SHPTSKRKRI NLGIYYNRNP LEVNCWSRPW 370 380 390 400 410 ILNRGPSGYS DLAVVEEQDL VACLFECGEK NEYERIDFCL FSDHEVLSCE DCTSPSSD

References

[1]	"Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation." Hasegawa T., Yamaguchi K., Wada T., Takeda A., Itoyama Y., Miyagi T. J. Biol. Chem. 275:8007-8015(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB026842 mRNA. Translation: BAA92868.1.
IPI	IPI00125683.
RefSeq	NP_057929.1. NM_016720.2.
UniGene	Mm.103703.
3D structure databases
ProteinModelPortal	Q9JMH7.
SMR	Q9JMH7. Positions 10-410.
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
PTM databases
PhosphoSite	Q9JMH7.
Proteomic databases
PRIDE	Q9JMH7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036331; ENSMUSP00000045222; ENSMUSG00000035239.
GeneID	50877.
KEGG	mmu:50877.
Organism-specific databases
CTD	10825.
MGI	MGI:1355305. Neu3.
Phylogenomic databases
eggNOG	NOG84455.
GeneTree	ENSGT00390000011171.
HOGENOM	HOG000233778.
HOVERGEN	HBG052608.
InParanoid	Q9JMH7.
KO	K12357.
OMA	VYLFFIC.
OrthoDB	EOG4FR0RV.
Gene expression databases
ArrayExpress	Q9JMH7.
Bgee	Q9JMH7.
CleanEx	MM_NEU3.
Genevestigator	Q9JMH7.
GermOnline	ENSMUSG00000035239. Mus musculus.
Family and domain databases
Gene3D	2.120.10.10. 1 hit.
InterPro	IPR026944. Sialidase-3. IPR026856. Sialidase_fam. IPR011040. Sialidases. [Graphical view]
PANTHER	PTHR10628. PTHR10628. 1 hit. PTHR10628:SF7. PTHR10628:SF7. 1 hit.
SUPFAM	SSF50939. Sialidase. 1 hit.
ProtoNet	Search...
Other
NextBio	307853.
SOURCE	Search...

Entry information

Entry name

NEUR3_MOUSE

Accession

Primary (citable) accession number: Q9JMH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program