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Q9JMH3 (NEUR2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-2

EC=3.2.1.18
Alternative name(s):
Cytosolic sialidase
Mouse skeletal muscle sialidase
Short name=MSS
Murine thymic sialidase
Short name=MTS
N-acetyl-alpha-neuraminidase 2
Gene names
Name:Neu2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length379 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates. Ref.1

Subcellular location

Cytoplasm.

Tissue specificity

Highly expressed in heart. Ref.2

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 2 BNR repeats.

Sequence caution

The sequence BAB39152.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 379379Sialidase-2
PRO_0000208900

Regions

Repeat127 – 13812BNR 1
Repeat197 – 20812BNR 2
Motif20 – 234FRIP motif

Sites

Active site461Proton acceptor By similarity
Active site3031 By similarity
Active site3331Nucleophile By similarity
Active site3541 Potential
Binding site211Substrate By similarity
Binding site411Substrate By similarity
Binding site1791Substrate By similarity
Binding site1811Substrate By similarity
Binding site2181Substrate By similarity
Binding site2371Substrate Potential
Binding site3031Substrate By similarity

Experimental info

Sequence conflict401K → R in AF139059. Ref.1
Sequence conflict91 – 977YDKQTKT → MTSKKD in AF139059. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9JMH3 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 02124A46398F6793

FASTA37942,403
        10         20         30         40         50         60 
MATCPVLQKE TLFRTGVHAY RIPALLYLKK QKTLLAFAEK RASKTDEHAE LIVLRRGSYN 

        70         80         90        100        110        120 
EATNRVKWQP EEVVTQAQLE GHRSMNPCPL YDKQTKTLFL FFIAVPGRVS EHHQLHTKVN 

       130        140        150        160        170        180 
VTRLCCVSST DHGRTWSPIQ DLTETTIGST HQEWATFAVG PGHCLQLRNP AGSLLVPAYA 

       190        200        210        220        230        240 
YRKLHPAQKP TPFAFCFISL DHGHTWKLGN FVAENSLECQ VAEVGTGAQR MVYLNARSFL 

       250        260        270        280        290        300 
GARVQAQSPN DGLDFQDNRV VSKLVEPPHG CHGSVVAFHN PISKPHALDT WLLYTHPTDS 

       310        320        330        340        350        360 
RNRTNLGVYL NQMPLDPTAW SEPTLLAMGI CAYSDLQNMG QGPDGSPQFG CLYESGNYEE 

       370 
IIFLIFTLKQ AFPTVFDAQ 

« Hide

References

[1]"Molecular cloning and expression of mouse brain sialidase."
Fronda C.L., Zeng G., Gao L., Yu R.K.
Biochem. Biophys. Res. Commun. 258:727-731(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY.
Tissue: Brain.
[2]"Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation."
Hasegawa T., Yamaguchi K., Wada T., Takeda A., Itoyama Y., Miyagi T.
J. Biol. Chem. 275:8007-8015(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Skeletal muscle.
[3]"Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase."
Kotani K., Kuroiwa A., Saito T., Matsuda Y., Koda T., Kijimoto-Ochiai S.
Biochem. Biophys. Res. Commun. 286:250-258(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF139059 mRNA. No translation available.
AB028023 mRNA. Translation: BAA92867.1.
AB048604 mRNA. Translation: BAB39152.1. Different initiation.
CCDSCCDS15135.1.
RefSeqNP_001153635.1. NM_001160163.1.
NP_001153636.1. NM_001160164.1.
NP_001153637.1. NM_001160165.1.
NP_056565.1. NM_015750.3.
XP_006529586.1. XM_006529523.1.
XP_006529587.1. XM_006529524.1.
UniGeneMm.143717.

3D structure databases

ProteinModelPortalQ9JMH3.
SMRQ9JMH3. Positions 1-376.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

PTM databases

PhosphoSiteQ9JMH3.

Proteomic databases

PaxDbQ9JMH3.
PRIDEQ9JMH3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000070898; ENSMUSP00000065439; ENSMUSG00000079434.
ENSMUST00000164128; ENSMUSP00000127913; ENSMUSG00000079434.
ENSMUST00000165109; ENSMUSP00000126509; ENSMUSG00000079434.
ENSMUST00000166259; ENSMUSP00000132513; ENSMUSG00000079434.
GeneID23956.
KEGGmmu:23956.
UCSCuc007bxa.2. mouse.

Organism-specific databases

CTD4759.
MGIMGI:1344417. Neu2.

Phylogenomic databases

eggNOGNOG84455.
GeneTreeENSGT00390000011171.
HOGENOMHOG000233778.
HOVERGENHBG052608.
InParanoidQ9JMH3.
KOK12357.
PhylomeDBQ9JMH3.
TreeFamTF331063.

Gene expression databases

ArrayExpressQ9JMH3.
BgeeQ9JMH3.
CleanExMM_NEU2.
GenevestigatorQ9JMH3.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026945. Sialidase-2.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF6. PTHR10628:SF6. 1 hit.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Other

NextBio303788.
PROQ9JMH3.
SOURCESearch...

Entry information

Entry nameNEUR2_MOUSE
AccessionPrimary (citable) accession number: Q9JMH3
Secondary accession number(s): Q99NA3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries