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Q9JMH3

- NEUR2_MOUSE

UniProt

Q9JMH3 - NEUR2_MOUSE

Protein

Sialidase-2

Gene

Neu2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei21 – 211SubstrateBy similarity
    Binding sitei41 – 411SubstrateBy similarity
    Active sitei46 – 461Proton acceptorBy similarity
    Binding sitei179 – 1791SubstrateBy similarity
    Binding sitei181 – 1811SubstrateBy similarity
    Binding sitei218 – 2181SubstrateBy similarity
    Binding sitei237 – 2371SubstrateSequence Analysis
    Active sitei303 – 3031By similarity
    Binding sitei303 – 3031SubstrateBy similarity
    Active sitei333 – 3331NucleophileBy similarity
    Active sitei354 – 3541Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. ganglioside catabolic process Source: UniProtKB
    2. oligosaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-2 (EC:3.2.1.18)
    Alternative name(s):
    Cytosolic sialidase
    Mouse skeletal muscle sialidase
    Short name:
    MSS
    Murine thymic sialidase
    Short name:
    MTS
    N-acetyl-alpha-neuraminidase 2
    Gene namesi
    Name:Neu2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1344417. Neu2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Sialidase-2PRO_0000208900Add
    BLAST

    Proteomic databases

    PaxDbiQ9JMH3.
    PRIDEiQ9JMH3.

    PTM databases

    PhosphoSiteiQ9JMH3.

    Expressioni

    Tissue specificityi

    Highly expressed in heart.1 Publication

    Gene expression databases

    ArrayExpressiQ9JMH3.
    BgeeiQ9JMH3.
    CleanExiMM_NEU2.
    GenevestigatoriQ9JMH3.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JMH3.
    SMRiQ9JMH3. Positions 1-376.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati127 – 13812BNR 1Add
    BLAST
    Repeati197 – 20812BNR 2Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi20 – 234FRIP motif

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 2 BNR repeats.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG84455.
    GeneTreeiENSGT00390000011171.
    HOGENOMiHOG000233778.
    HOVERGENiHBG052608.
    InParanoidiQ9JMH3.
    KOiK12357.
    PhylomeDBiQ9JMH3.
    TreeFamiTF331063.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026945. Sialidase-2.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF6. PTHR10628:SF6. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9JMH3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATCPVLQKE TLFRTGVHAY RIPALLYLKK QKTLLAFAEK RASKTDEHAE    50
    LIVLRRGSYN EATNRVKWQP EEVVTQAQLE GHRSMNPCPL YDKQTKTLFL 100
    FFIAVPGRVS EHHQLHTKVN VTRLCCVSST DHGRTWSPIQ DLTETTIGST 150
    HQEWATFAVG PGHCLQLRNP AGSLLVPAYA YRKLHPAQKP TPFAFCFISL 200
    DHGHTWKLGN FVAENSLECQ VAEVGTGAQR MVYLNARSFL GARVQAQSPN 250
    DGLDFQDNRV VSKLVEPPHG CHGSVVAFHN PISKPHALDT WLLYTHPTDS 300
    RNRTNLGVYL NQMPLDPTAW SEPTLLAMGI CAYSDLQNMG QGPDGSPQFG 350
    CLYESGNYEE IIFLIFTLKQ AFPTVFDAQ 379
    Length:379
    Mass (Da):42,403
    Last modified:October 1, 2000 - v1
    Checksum:i02124A46398F6793
    GO

    Sequence cautioni

    The sequence BAB39152.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti40 – 401K → R in AF139059. (PubMed:10329453)Curated
    Sequence conflicti91 – 977YDKQTKT → MTSKKD in AF139059. (PubMed:10329453)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF139059 mRNA. No translation available.
    AB028023 mRNA. Translation: BAA92867.1.
    AB048604 mRNA. Translation: BAB39152.1. Different initiation.
    CCDSiCCDS15135.1.
    RefSeqiNP_001153635.1. NM_001160163.1.
    NP_001153636.1. NM_001160164.1.
    NP_001153637.1. NM_001160165.1.
    NP_056565.1. NM_015750.3.
    XP_006529586.1. XM_006529523.1.
    XP_006529587.1. XM_006529524.1.
    UniGeneiMm.143717.

    Genome annotation databases

    EnsembliENSMUST00000070898; ENSMUSP00000065439; ENSMUSG00000079434.
    ENSMUST00000164128; ENSMUSP00000127913; ENSMUSG00000079434.
    ENSMUST00000165109; ENSMUSP00000126509; ENSMUSG00000079434.
    ENSMUST00000166259; ENSMUSP00000132513; ENSMUSG00000079434.
    GeneIDi23956.
    KEGGimmu:23956.
    UCSCiuc007bxa.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF139059 mRNA. No translation available.
    AB028023 mRNA. Translation: BAA92867.1 .
    AB048604 mRNA. Translation: BAB39152.1 . Different initiation.
    CCDSi CCDS15135.1.
    RefSeqi NP_001153635.1. NM_001160163.1.
    NP_001153636.1. NM_001160164.1.
    NP_001153637.1. NM_001160165.1.
    NP_056565.1. NM_015750.3.
    XP_006529586.1. XM_006529523.1.
    XP_006529587.1. XM_006529524.1.
    UniGenei Mm.143717.

    3D structure databases

    ProteinModelPortali Q9JMH3.
    SMRi Q9JMH3. Positions 1-376.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    PTM databases

    PhosphoSitei Q9JMH3.

    Proteomic databases

    PaxDbi Q9JMH3.
    PRIDEi Q9JMH3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000070898 ; ENSMUSP00000065439 ; ENSMUSG00000079434 .
    ENSMUST00000164128 ; ENSMUSP00000127913 ; ENSMUSG00000079434 .
    ENSMUST00000165109 ; ENSMUSP00000126509 ; ENSMUSG00000079434 .
    ENSMUST00000166259 ; ENSMUSP00000132513 ; ENSMUSG00000079434 .
    GeneIDi 23956.
    KEGGi mmu:23956.
    UCSCi uc007bxa.2. mouse.

    Organism-specific databases

    CTDi 4759.
    MGIi MGI:1344417. Neu2.

    Phylogenomic databases

    eggNOGi NOG84455.
    GeneTreei ENSGT00390000011171.
    HOGENOMi HOG000233778.
    HOVERGENi HBG052608.
    InParanoidi Q9JMH3.
    KOi K12357.
    PhylomeDBi Q9JMH3.
    TreeFami TF331063.

    Enzyme and pathway databases

    Reactomei REACT_199008. Glycosphingolipid metabolism.
    REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 303788.
    PROi Q9JMH3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JMH3.
    Bgeei Q9JMH3.
    CleanExi MM_NEU2.
    Genevestigatori Q9JMH3.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026945. Sialidase-2.
    IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    PTHR10628:SF6. PTHR10628:SF6. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of mouse brain sialidase."
      Fronda C.L., Zeng G., Gao L., Yu R.K.
      Biochem. Biophys. Res. Commun. 258:727-731(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY.
      Tissue: Brain.
    2. "Molecular cloning of mouse ganglioside sialidase and its increased expression in Neuro2a cell differentiation."
      Hasegawa T., Yamaguchi K., Wada T., Takeda A., Itoyama Y., Miyagi T.
      J. Biol. Chem. 275:8007-8015(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Tissue: Skeletal muscle.
    3. "Cloning, chromosomal mapping, and characteristic 5'-UTR sequence of murine cytosolic sialidase."
      Kotani K., Kuroiwa A., Saito T., Matsuda Y., Koda T., Kijimoto-Ochiai S.
      Biochem. Biophys. Res. Commun. 286:250-258(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Thymus.

    Entry informationi

    Entry nameiNEUR2_MOUSE
    AccessioniPrimary (citable) accession number: Q9JMH3
    Secondary accession number(s): Q99NA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 16, 2001
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3