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Protein

Transmembrane and ubiquitin-like domain-containing protein 1

Gene

Tmub1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in sterol-regulated ubiquitination and degradation of HMG-CoA reductase HMGCR (By similarity). Involved in positive regulation of AMPA-selective glutamate receptor GRIA2 recycling to the cell surface (By similarity). Acts as negative regulator of hepatocyte growth during regeneration (By similarity).By similarity3 Publications
iHOPS: May contribute to the regulation of translation during cell-cycle progression. May contribute to the regulation of cell proliferation (PubMed:16014383). May be involved in centrosome assembly (PubMed:18418082). Modulates stabilization and nucleolar localization of tumor suppressor CDKN2A and enhances association between CDKN2A and NPM1 (PubMed:22890319).2 Publications

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Transmembrane and ubiquitin-like domain-containing protein 1
Alternative name(s):
Hepatocyte odd protein shuttling protein
Cleaved into the following chain:
iHOPS1 Publication
Gene namesi
Name:Tmub1
Synonyms:Hops
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923764. Tmub1.

Subcellular locationi

Isoform 1 :
  • Membrane By similarity1 Publication
Isoform 2 :
  • Membrane By similarity1 Publication
iHOPS :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei11 – 31HelicalSequence analysisAdd BLAST21
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoskeleton, Endosome, Membrane, Nucleus, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Disruption phenotypei

Strong increase in home cage locomotor activity during the dark phase (subjective day) of the light:dark (L:D) cycle. There were no changes in activity during the light period and in locomotor activity observed in other assays, e.g. novel open-field.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000435489? – 245iHOPS
ChainiPRO_00001149231 – 245Transmembrane and ubiquitin-like domain-containing protein 1Add BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineBy similarity1
Modified residuei97PhosphoserineBy similarity1
Modified residuei126PhosphoserineBy similarity1

Post-translational modificationi

iHOPS: Isoform 1 (lHOPS) is processed by regulated intramembrane proteolysis (RIP) in the N-terminus to release iHOPS from membranes.1 Publication
Isoform 2 seems to undergo a selective cleavage in the C-terminal region to release an additional cytoplasmic form.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JMG3.
PaxDbiQ9JMG3.
PRIDEiQ9JMG3.

PTM databases

iPTMnetiQ9JMG3.
PhosphoSitePlusiQ9JMG3.

Expressioni

Tissue specificityi

Expressed in adult brain; at protein level (PubMed:18665261, PubMed:20582322). Isoform 1 (lHOPS) is highly expressed in small intestine, stomach and epididymis. Isoform 2 (sHOPS) and iHOPS are abundantly expressed in brain, liver and adrenal gland (PubMed:24240191).2 Publications

Inductioni

Up-regulated in regenerating liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000028958.
ExpressionAtlasiQ9JMG3. baseline and differential.
GenevisibleiQ9JMG3. MM.

Interactioni

Subunit structurei

Interacts with EEF1A1, CAMLG, GRIA2 and GRIP1. Interacts with NPM1 and CDKN2A; TMUB1 can enhance interaction between NPM1 and CDKN2A and is proposed to bridge the proteins; proposed to be mediated by iHOPS (PubMed:16014383, PubMed:18665261, PubMed:20582322, PubMed:22890319, PubMed:24240191). Interacts with TUBG1 (By similarity). Interacts with ERLIN2 and AMFR; TMUB1 promotes the interaction of ERLIN2 with AMFR (By similarity).By similarity5 Publications

Protein-protein interaction databases

BioGridi211057. 2 interactors.
IntActiQ9JMG3. 3 interactors.
MINTiMINT-4090680.
STRINGi10090.ENSMUSP00000030799.

Structurei

3D structure databases

ProteinModelPortaliQ9JMG3.
SMRiQ9JMG3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini102 – 175Ubiquitin-likePROSITE-ProRule annotationAdd BLAST74

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 30Required to release iHOPS from membranes1 PublicationAdd BLAST29

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG1T. Eukaryota.
ENOG4111WH7. LUCA.
GeneTreeiENSGT00390000014069.
HOGENOMiHOG000232067.
HOVERGENiHBG050920.
InParanoidiQ9JMG3.
PhylomeDBiQ9JMG3.
TreeFamiTF329265.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9JMG3-1) [UniParc]FASTAAdd to basket
Also known as: lHOPS

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALIEGVGDE VTVLFAVLAC LLVLALAWVS THTTESTDPQ PQPPGTTTPA
60 70 80 90 100
QPSEAMSASD SIREEAPGAE SPSLRHRGPS AQPEPDTGVT ASTPPDSPQE
110 120 130 140 150
PLLLRLKFLN DSEQVARAWP QDTIGSLKRT QFPGQEQQVR LIYQGQLLGD
160 170 180 190 200
DTQTLGSLHL PPNCVLHCHV STRVGPPHPP CPPGSEPGPS GLEIGSLLLP
210 220 230 240
LLLLLLLLLW YCQIQYRPFF PLTATLGLAG FTLLLSLLAF AMYRP
Length:245
Mass (Da):26,316
Last modified:October 1, 2000 - v1
Checksum:i02C1D1B24C13139D
GO
Isoform 2 (identifier: Q9JMG3-2) [UniParc]FASTAAdd to basket
Also known as: sHOPS

The sequence of this isoform differs from the canonical sequence as follows:
     1-55: Missing.

Show »
Length:190
Mass (Da):20,659
Checksum:i2CF0794030B77FA8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti233L → Q in AAU00155 (PubMed:16014383).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0581021 – 55Missing in isoform 2. 1 PublicationAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030183 mRNA. Translation: BAA92747.1.
AY603379 mRNA. Translation: AAU00155.1.
AK008109 mRNA. Translation: BAB25465.1.
BC019547 mRNA. Translation: AAH19547.1.
CCDSiCCDS19121.1. [Q9JMG3-1]
RefSeqiNP_071863.1. NM_022418.3. [Q9JMG3-1]
XP_006535849.1. XM_006535786.2. [Q9JMG3-1]
UniGeneiMm.150098.

Genome annotation databases

EnsembliENSMUST00000030799; ENSMUSP00000030799; ENSMUSG00000028958. [Q9JMG3-1]
ENSMUST00000115033; ENSMUSP00000110685; ENSMUSG00000028958. [Q9JMG3-1]
GeneIDi64295.
KEGGimmu:64295.
UCSCiuc008wrt.2. mouse. [Q9JMG3-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030183 mRNA. Translation: BAA92747.1.
AY603379 mRNA. Translation: AAU00155.1.
AK008109 mRNA. Translation: BAB25465.1.
BC019547 mRNA. Translation: AAH19547.1.
CCDSiCCDS19121.1. [Q9JMG3-1]
RefSeqiNP_071863.1. NM_022418.3. [Q9JMG3-1]
XP_006535849.1. XM_006535786.2. [Q9JMG3-1]
UniGeneiMm.150098.

3D structure databases

ProteinModelPortaliQ9JMG3.
SMRiQ9JMG3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211057. 2 interactors.
IntActiQ9JMG3. 3 interactors.
MINTiMINT-4090680.
STRINGi10090.ENSMUSP00000030799.

PTM databases

iPTMnetiQ9JMG3.
PhosphoSitePlusiQ9JMG3.

Proteomic databases

MaxQBiQ9JMG3.
PaxDbiQ9JMG3.
PRIDEiQ9JMG3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030799; ENSMUSP00000030799; ENSMUSG00000028958. [Q9JMG3-1]
ENSMUST00000115033; ENSMUSP00000110685; ENSMUSG00000028958. [Q9JMG3-1]
GeneIDi64295.
KEGGimmu:64295.
UCSCiuc008wrt.2. mouse. [Q9JMG3-1]

Organism-specific databases

CTDi83590.
MGIiMGI:1923764. Tmub1.

Phylogenomic databases

eggNOGiENOG410IG1T. Eukaryota.
ENOG4111WH7. LUCA.
GeneTreeiENSGT00390000014069.
HOGENOMiHOG000232067.
HOVERGENiHBG050920.
InParanoidiQ9JMG3.
PhylomeDBiQ9JMG3.
TreeFamiTF329265.

Miscellaneous databases

ChiTaRSiTmub1. mouse.
PROiQ9JMG3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028958.
ExpressionAtlasiQ9JMG3. baseline and differential.
GenevisibleiQ9JMG3. MM.

Family and domain databases

InterProiIPR029071. Ubiquitin-rel_dom.
IPR000626. Ubiquitin_dom.
[Graphical view]
PfamiPF00240. ubiquitin. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS50053. UBIQUITIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTMUB1_MOUSE
AccessioniPrimary (citable) accession number: Q9JMG3
Secondary accession number(s): Q53AQ3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.