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Protein

High affinity choline transporter 1

Gene

Slc5a7

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transmembrane transporter that imports choline from the extracellular space to the neuron with high affinity. Choline uptake is the rate-limiting step in acetylcholine synthesis. Sodium ion- and chloride ion-dependent.1 Publication

Miscellaneous

Specifically inhibited by nanomolar concentrations of hemicholinium 3.

GO - Molecular functioni

  • choline:sodium symporter activity Source: GO_Central
  • choline binding Source: GO_Central
  • choline transmembrane transporter activity Source: UniProtKB

GO - Biological processi

  • acetylcholine biosynthetic process Source: UniProtKB
  • neuromuscular synaptic transmission Source: GO_Central
  • synaptic transmission, cholinergic Source: GO_Central

Keywordsi

Biological processIon transport, Neurotransmitter biosynthesis, Sodium transport, Symport, Transport
LigandSodium

Protein family/group databases

TCDBi2.A.21.8.1 the solute:sodium symporter (sss) family

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity choline transporter 1
Alternative name(s):
Hemicholinium-3-sensitive choline transporter
Short name:
CHT
Solute carrier family 5 member 7
Gene namesi
Name:Slc5a7
Synonyms:Cht1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69270 Slc5a7

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6ExtracellularSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 48CytoplasmicSequence analysisAdd BLAST21
Transmembranei49 – 69HelicalSequence analysisAdd BLAST21
Topological domaini70 – 81ExtracellularSequence analysisAdd BLAST12
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 125CytoplasmicSequence analysisAdd BLAST23
Transmembranei126 – 146HelicalSequence analysisAdd BLAST21
Topological domaini147 – 164ExtracellularSequence analysisAdd BLAST18
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 191CytoplasmicSequence analysis6
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 237ExtracellularSequence analysisAdd BLAST25
Transmembranei238 – 258HelicalSequence analysisAdd BLAST21
Topological domaini259 – 274CytoplasmicSequence analysisAdd BLAST16
Transmembranei275 – 295HelicalSequence analysisAdd BLAST21
Topological domaini296 – 317ExtracellularSequence analysisAdd BLAST22
Transmembranei318 – 338HelicalSequence analysisAdd BLAST21
Topological domaini339 – 376CytoplasmicSequence analysisAdd BLAST38
Transmembranei377 – 397HelicalSequence analysisAdd BLAST21
Topological domaini398 – 406ExtracellularSequence analysis9
Transmembranei407 – 427HelicalSequence analysisAdd BLAST21
Topological domaini428 – 435CytoplasmicSequence analysis8
Transmembranei436 – 456HelicalSequence analysisAdd BLAST21
Topological domaini457 – 481ExtracellularSequence analysisAdd BLAST25
Transmembranei482 – 502HelicalSequence analysisAdd BLAST21
Topological domaini503 – 580CytoplasmicSequence analysisAdd BLAST78

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5797

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001053931 – 580High affinity choline transporter 1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi301N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9JMD7
PRIDEiQ9JMD7

PTM databases

iPTMnetiQ9JMD7
PhosphoSitePlusiQ9JMD7

Expressioni

Tissue specificityi

Expressed in basal forebrain, brain stem, spinal chord, and striatum. Specific for cholinergic neurons.1 Publication

Interactioni

Subunit structurei

Homooligomerizes at cell surface (By similarity). Interacts with SEC14L1; may regulate SLC5A7 (PubMed:17092608).By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000014548

Chemistry databases

BindingDBiQ9JMD7

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni502 – 580Mediates interaction with SEC14L11 PublicationAdd BLAST79

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3761 Eukaryota
COG0591 LUCA
HOGENOMiHOG000016386
HOVERGENiHBG054160
InParanoidiQ9JMD7
KOiK14387
PhylomeDBiQ9JMD7

Family and domain databases

Gene3Di1.20.1730.10, 1 hit
InterProiView protein in InterPro
IPR038377 Na/Glc_symporter_sf
IPR001734 Na/solute_symporter
PfamiView protein in Pfam
PF00474 SSF, 1 hit
PROSITEiView protein in PROSITE
PS50283 NA_SOLUT_SYMP_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JMD7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFHVEGLVA IILFYLLIFL VGIWAAWKTK NSGNAEERSE AIIVGGRDIG
60 70 80 90 100
LLVGGFTMTA TWVGGGYING TAEAVYGPGC GLAWAQAPIG YSLSLILGGL
110 120 130 140 150
FFAKPMRSKG YVTMLDPFQQ IYGKRMGGLL FIPALMGEMF WAAAIFSALG
160 170 180 190 200
ATISVIIDVD VNISVIVSAL IAILYTLVGG LYSVAYTDVV QLFCIFIGLW
210 220 230 240 250
ISVPFALSHP AVTDIGFTAV HAKYQSPWLG TIESVEVYTW LDNFLLLMLG
260 270 280 290 300
GIPWQAYFQR VLSSSSATYA QVLSFLAAFG CLVMALPAIC IGAIGASTDW
310 320 330 340 350
NQTAYGFPDP KTKEEADMIL PIVLQYLCPV YISFFGLGAV SAAVMSSADS
360 370 380 390 400
SILSASSMFA RNIYQLSFRQ NASDKEIVWV MRITVFVFGA SATAMALLTK
410 420 430 440 450
TVYGLWYLSS DLVYIIIFPQ LLCVLFIKGT NTYGAVAGYI FGLFLRITGG
460 470 480 490 500
EPYLYLQPLI FYPGYYPDKN GIYNQRFPFK TLSMVTSFFT NICVSYLAKY
510 520 530 540 550
LFESGTLPPK LDIFDAVVSR HSEENMDKTI LVRNENIKLN ELAPVKPRQS
560 570 580
LTLSSTFTNK EALLDVDSSP EGSGTEDNLQ
Length:580
Mass (Da):63,407
Last modified:October 1, 2000 - v1
Checksum:iB7CB73323DAD17A7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB030947 mRNA Translation: BAA90484.1
RefSeqiNP_445973.1, NM_053521.1
UniGeneiRn.44872

Genome annotation databases

GeneIDi85426
KEGGirno:85426
UCSCiRGD:69270 rat

Similar proteinsi

Entry informationi

Entry nameiSC5A7_RAT
AccessioniPrimary (citable) accession number: Q9JMD7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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