Q9JMD3 (PCTL_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
PCTP-like protein
Short name=PCTP-L

Alternative name(s):
START domain-containing protein 10
Short name=StARD10
Serologically defined colon cancer antigen 28 homolog
StAR-related lipid transfer protein 10

Gene names

Name:	Stard10
Synonyms:	Pctpl, Sdccag28, Sdccagg28

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Phospholipid transfer protein that preferentially selects lipid species containing a palmitoyl or stearoyl chain on the sn-1 and an unsaturated fatty acyl chain (18:1 or 18:2) on the sn-2 position. Able to transfer phosphatidylcholine (PC) and phosphatidyetanolamline (PE) between membranes By similarity. May play metabolic roles in sperm maturation or fertilization. Ref.1
Subcellular location	Cell projection › cilium › flagellum. Cytoplasm By similarity. Membrane By similarity. Note: Mainly cytosolic By similarity. In testis was predominantly detected at the flagella of elongated spermatids, with a strong signal also found at the tail of epididymal sperm. Ref.1
Tissue specificity	Testis, kidney, liver, and intestine with the highest level in the testis.
Developmental stage	During male germ cell development, it was detected first in the 23-day-old mouse testis, and the signal increased with age.
Post-translational modification	Phosphorylation at Ser-284 by CK2 negatively regulates lipid transfer activity, possibly by decreasing membrane association By similarity.
Sequence similarities	Contains 1 START domain.

Ontologies

Keywords
Biological process	Lipid transport Transport
Cellular component	Cell projection Cilium Cytoplasm Flagellum Membrane
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cilium Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	lipid binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	PCTP-like protein		PRO_0000220662
Regions
	Domain	14 – 224	211	START
Amino acid modifications
	Modified residue	13	1	Phosphoserine Ref.3
	Modified residue	284	1	Phosphoserine; by CK2 Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9JMD3 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 598467C430D97080	FASTA	291	32,951
	10 20 30 40 50 60 MEKPAASTEP QGSRPALGRE SVQVPDDQDF RSFRSECEAE VGWNLTYSKA GVSVWVQAVE 70 80 90 100 110 120 MDRTLHKIKC RMECCDVPAE TLYDVLHDIE YRKKWDSNVI ETFDIARLTV NADVGYYSWR 130 140 150 160 170 180 CPKPLKNRDV ITLRSWLPMG ADYIIMNYSV KHPKYPPRKD LVRAVSIQTG YLIQSTGPKS 190 200 210 220 230 240 CVITYLAQVD PKGSLPKWVV NKSSQFLAPK AMKKMYKACI KYPEWKQKHQ PHFKPWLHPE 250 260 270 280 290 QSPLPSLALS ELSVQHADSL ENIDESAVTE SREERAGGAG GEGSDDDTSL T

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of phosphatidylcholine transfer protein-like protein gene expressed in murine haploid germ cells." Yamanaka M., Koga M., Tanaka H., Nakamura Y., Ohta H., Yomogida K., Tsuchida J., Iguchi N., Nojima H., Nozaki M., Matsumiya K., Okuyama A., Toshimori K., Nishimune Y. Biol. Reprod. 62:1694-1701(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION. Tissue: Testis.
[2]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-284, MASS SPECTROMETRY. Tissue: Liver.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-13, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB031550 mRNA. Translation: BAA92233.1.
IPI	IPI00125515.
RefSeq	NP_064374.1. NM_019990.4.
UniGene	Mm.28896.
3D structure databases
ProteinModelPortal	Q9JMD3.
SMR	Q9JMD3. Positions 42-198.
ModBase	Search...
PTM databases
PhosphoSite	Q9JMD3.
Proteomic databases
PaxDb	Q9JMD3.
PRIDE	Q9JMD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032927; ENSMUSP00000032927; ENSMUSG00000030688. ENSMUST00000164479; ENSMUSP00000133002; ENSMUSG00000030688.
GeneID	56018.
KEGG	mmu:56018.
Organism-specific databases
CTD	10809.
MGI	MGI:1860093. Stard10.
Phylogenomic databases
eggNOG	NOG292439.
GeneTree	ENSGT00510000047611.
HOVERGEN	HBG031807.
InParanoid	Q9JMD3.
OrthoDB	EOG4001K5.
Gene expression databases
ArrayExpress	Q9JMD3.
Bgee	Q9JMD3.
CleanEx	MM_STARD10.
Genevestigator	Q9JMD3.
GermOnline	ENSMUSG00000030688. Mus musculus.
Family and domain databases
Gene3D	3.30.530.20. 1 hit.
InterPro	IPR023393. START-like_dom. IPR002913. START_lipid-bd_dom. [Graphical view]
Pfam	PF01852. START. 1 hit. [Graphical view]
SMART	SM00234. START. 1 hit. [Graphical view]
PROSITE	PS50848. START. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	STARD10. mouse.
NextBio	311762.
SOURCE	Search...

Entry information

Entry name

PCTL_MOUSE

Accession

Primary (citable) accession number: Q9JMD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program