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Protein

BUB3-interacting and GLEBS motif-containing protein ZNF207

Gene

Znf207

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Kinetochore- and microtubule-binding protein that plays a key role in spindle assembly. ZNF207/BuGZ is mainly composed of disordered low-complexity regions and undergoes phase transition or coacervation to form temperature-dependent liquid droplets. Coacervation promotes microtubule bundling and concentrates tubulin, promoting microtubule polymerization and assembly of spindle and spindle matrix by concentrating its building blocks (PubMed:26388440). Also acts as a regulator of mitotic chromosome alignment by mediating the stability and kinetochore loading of BUB3. Mechanisms by which BUB3 is protected are unclear: according to a first report, ZNF207/BuGZ may act by blocking ubiquitination and proteasomal degradation of BUB3. According to another report, the stabilization is independent of the proteasome (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 3424C2H2-type 1Add
BLAST
Zinc fingeri35 – 5824C2H2-type 2Add
BLAST

GO - Molecular functioni

  • heparin binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • microtubule binding Source: UniProtKB
  • poly(A) RNA binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Chromosome partition, Mitosis

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
BUB3-interacting and GLEBS motif-containing protein ZNF207By similarity
Short name:
BuGZBy similarity
Alternative name(s):
49 kDa zinc finger protein
Zinc finger protein 207By similarity
Gene namesi
Name:Znf207By similarity
Synonyms:BugzBy similarity, Zep1 Publication, Zfp207Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1340045. Zfp207.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosomecentromerekinetochore By similarity
  • Cytoplasmcytoskeletonspindle By similarity

  • Note: Localizes primarily to the nucleus in interphase, concentrates at kinetochores prior to nuclear envelope breakdown and during early prometaphase, and disappears from kinetochores upon microtubule-binding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi390 – 3912EE → AA: Abolishes interaction and stabilization of BUB3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495BUB3-interacting and GLEBS motif-containing protein ZNF207PRO_0000428733Add
BLAST

Proteomic databases

MaxQBiQ9JMD0.
PaxDbiQ9JMD0.
PeptideAtlasiQ9JMD0.
PRIDEiQ9JMD0.

PTM databases

iPTMnetiQ9JMD0.
PhosphoSiteiQ9JMD0.

Expressioni

Tissue specificityi

In day-13 embryo, strongly expressed in the nervous system (brain, spinal cord and dorsal root ganglia), with strong to weak expression in other regions. Continues to be strongly expressed in the neonatal brain while expression is weak in the brain and spinal cord of adult.1 Publication

Gene expression databases

BgeeiENSMUSG00000017421.
ExpressionAtlasiQ9JMD0. baseline and differential.
GenevisibleiQ9JMD0. MM.

Interactioni

Subunit structurei

Interacts (via GLEBS region) with BUB3.1 Publication

GO - Molecular functioni

  • microtubule binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9JMD0. 2 interactions.
MINTiMINT-4131445.
STRINGi10090.ENSMUSP00000054168.

Structurei

3D structure databases

ProteinModelPortaliQ9JMD0.
SMRiQ9JMD0. Positions 10-61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 9292Microtubule-binding regionBy similarityAdd
BLAST
Regioni376 – 40833GLEBSBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi4 – 74Poly-Lys
Compositional biasi129 – 487359Pro-richAdd
BLAST
Compositional biasi326 – 37752Thr-richAdd
BLAST

Domaini

Mainly composed of disordered low-complexity regions outside of the C2H2-type zinc fingers. Coacervation depends on hydrophobic and aromatic Phe and Tyr in the disordered low-complexity region, that may promote coacervation by forming intermolecular hydrophobic interactions.1 Publication
The GLEBS region mediates interaction with BUB3.By similarity
The microtubule-binding region is required for efficient loading of BUB3 onto kinetochores and proper mitosis.By similarity

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri11 – 3424C2H2-type 1Add
BLAST
Zinc fingeri35 – 5824C2H2-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2893. Eukaryota.
ENOG410ZJGQ. LUCA.
GeneTreeiENSGT00730000111057.
HOGENOMiHOG000013124.
HOVERGENiHBG054182.
InParanoidiQ9JMD0.
OMAiCGGNMMM.
OrthoDBiEOG091G0N0A.
PhylomeDBiQ9JMD0.
TreeFamiTF313844.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JMD0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGRKKKKQLK PWCWYCNRDF DDEKILIQHQ KAKHFKCHIC HKKLYTGPGL
60 70 80 90 100
AIHCMQVHKE TIDAVPNAIP GRTDIELEIY GMEGIPEKDM DERRRLLEQK
110 120 130 140 150
TQESQKKKQQ DDSDEYDDDE SAASTSFQPQ PVQPQQGYIP PMAQPGLPPV
160 170 180 190 200
PGAPGMPPGI PPLMPGVPPL MPGMPPVMPG MPPGLHHQRK YTQSFCGENI
210 220 230 240 250
MMPMGGMMPP GPGIPPLMPG MPPGMPPPVP RPGIPPMTQA QAVSAPGILN
260 270 280 290 300
RPPAPTAAVP APQPPVTKPL FPSAGQMGTP VTSSSTASSN SESLSASSKA
310 320 330 340 350
LFPSTAQAQA AVQGPVGTDF KPLNSTPAAT TTEPPKPTFP AYTQSTASTT
360 370 380 390 400
STTNSTAAKP AASITSKPAT LTTTSATSKL IHPDEDISLE ERRAQLPKYQ
410 420 430 440 450
RNLPRPGQTP IGNPPVGPIG GMMPPQPGLP QQQAMRPPMP PHGQYGGHHQ
460 470 480 490
GMPGYLPGAM PPYGQGPPMV PPYQGGPPRP PMGMRPPVMS QGGRY
Length:495
Mass (Da):52,793
Last modified:October 1, 2000 - v1
Checksum:i71EB32147BB6A5AD
GO
Isoform 2 (identifier: Q9JMD0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     185-200: Missing.

Show »
Length:479
Mass (Da):50,850
Checksum:i5C1F3F5AEF7C9BB6
GO
Isoform 3 (identifier: Q9JMD0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     276-306: Missing.

Show »
Length:464
Mass (Da):49,792
Checksum:iE1486181B179DEE4
GO
Isoform 4 (identifier: Q9JMD0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     219-222: Missing.
     276-306: Missing.

Show »
Length:460
Mass (Da):49,409
Checksum:i03A7D7302B45D6E7
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei185 – 20016Missing in isoform 2. CuratedVSP_054255Add
BLAST
Alternative sequencei219 – 2224Missing in isoform 4. 1 PublicationVSP_054256
Alternative sequencei276 – 30631Missing in isoform 3 and isoform 4. 3 PublicationsVSP_054257Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013357 mRNA. Translation: BAA37094.1.
AB032196 mRNA. Translation: BAA92375.1.
AK087885 mRNA. Translation: BAC40034.1.
AK146787 mRNA. Translation: BAE27431.1.
AK159683 mRNA. Translation: BAE35286.1.
AL591146 Genomic DNA. No translation available.
CH466556 Genomic DNA. Translation: EDL15636.1.
CH466556 Genomic DNA. Translation: EDL15638.1.
BC003715 mRNA. Translation: AAH03715.1.
CCDSiCCDS48863.1. [Q9JMD0-1]
CCDS48864.1. [Q9JMD0-3]
CCDS48865.1. [Q9JMD0-2]
PIRiJE0367.
RefSeqiNP_001123641.1. NM_001130169.1. [Q9JMD0-1]
NP_001123642.1. NM_001130170.1. [Q9JMD0-2]
NP_001123643.1. NM_001130171.1. [Q9JMD0-3]
NP_035881.1. NM_011751.3. [Q9JMD0-4]
XP_006533252.1. XM_006533189.1. [Q9JMD0-1]
UniGeneiMm.401332.
Mm.440925.
Mm.456081.
Mm.468199.

Genome annotation databases

EnsembliENSMUST00000017567; ENSMUSP00000017567; ENSMUSG00000017421. [Q9JMD0-3]
ENSMUST00000053740; ENSMUSP00000054168; ENSMUSG00000017421. [Q9JMD0-1]
ENSMUST00000165565; ENSMUSP00000132968; ENSMUSG00000017421. [Q9JMD0-2]
ENSMUST00000178665; ENSMUSP00000136727; ENSMUSG00000017421. [Q9JMD0-3]
ENSMUST00000188489; ENSMUSP00000139653; ENSMUSG00000017421. [Q9JMD0-3]
GeneIDi22680.
KEGGimmu:22680.
UCSCiuc007kly.2. mouse. [Q9JMD0-4]
uc007klz.2. mouse. [Q9JMD0-1]
uc007kma.2. mouse. [Q9JMD0-3]
uc007kmb.2. mouse. [Q9JMD0-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013357 mRNA. Translation: BAA37094.1.
AB032196 mRNA. Translation: BAA92375.1.
AK087885 mRNA. Translation: BAC40034.1.
AK146787 mRNA. Translation: BAE27431.1.
AK159683 mRNA. Translation: BAE35286.1.
AL591146 Genomic DNA. No translation available.
CH466556 Genomic DNA. Translation: EDL15636.1.
CH466556 Genomic DNA. Translation: EDL15638.1.
BC003715 mRNA. Translation: AAH03715.1.
CCDSiCCDS48863.1. [Q9JMD0-1]
CCDS48864.1. [Q9JMD0-3]
CCDS48865.1. [Q9JMD0-2]
PIRiJE0367.
RefSeqiNP_001123641.1. NM_001130169.1. [Q9JMD0-1]
NP_001123642.1. NM_001130170.1. [Q9JMD0-2]
NP_001123643.1. NM_001130171.1. [Q9JMD0-3]
NP_035881.1. NM_011751.3. [Q9JMD0-4]
XP_006533252.1. XM_006533189.1. [Q9JMD0-1]
UniGeneiMm.401332.
Mm.440925.
Mm.456081.
Mm.468199.

3D structure databases

ProteinModelPortaliQ9JMD0.
SMRiQ9JMD0. Positions 10-61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9JMD0. 2 interactions.
MINTiMINT-4131445.
STRINGi10090.ENSMUSP00000054168.

PTM databases

iPTMnetiQ9JMD0.
PhosphoSiteiQ9JMD0.

Proteomic databases

MaxQBiQ9JMD0.
PaxDbiQ9JMD0.
PeptideAtlasiQ9JMD0.
PRIDEiQ9JMD0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017567; ENSMUSP00000017567; ENSMUSG00000017421. [Q9JMD0-3]
ENSMUST00000053740; ENSMUSP00000054168; ENSMUSG00000017421. [Q9JMD0-1]
ENSMUST00000165565; ENSMUSP00000132968; ENSMUSG00000017421. [Q9JMD0-2]
ENSMUST00000178665; ENSMUSP00000136727; ENSMUSG00000017421. [Q9JMD0-3]
ENSMUST00000188489; ENSMUSP00000139653; ENSMUSG00000017421. [Q9JMD0-3]
GeneIDi22680.
KEGGimmu:22680.
UCSCiuc007kly.2. mouse. [Q9JMD0-4]
uc007klz.2. mouse. [Q9JMD0-1]
uc007kma.2. mouse. [Q9JMD0-3]
uc007kmb.2. mouse. [Q9JMD0-2]

Organism-specific databases

CTDi22680.
MGIiMGI:1340045. Zfp207.

Phylogenomic databases

eggNOGiKOG2893. Eukaryota.
ENOG410ZJGQ. LUCA.
GeneTreeiENSGT00730000111057.
HOGENOMiHOG000013124.
HOVERGENiHBG054182.
InParanoidiQ9JMD0.
OMAiCGGNMMM.
OrthoDBiEOG091G0N0A.
PhylomeDBiQ9JMD0.
TreeFamiTF313844.

Miscellaneous databases

PROiQ9JMD0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000017421.
ExpressionAtlasiQ9JMD0. baseline and differential.
GenevisibleiQ9JMD0. MM.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiZN207_MOUSE
AccessioniPrimary (citable) accession number: Q9JMD0
Secondary accession number(s): E9PW12, Q99LA2, Q9Z326
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2014
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.