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Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

Usp14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Maintains the cellular levels of monomeric ubiquitin in cells. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor cxcr4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1.2 Publications

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei114NucleophilePROSITE-ProRule annotation1
Active sitei434Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.

Protein family/group databases

MEROPSiC19.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 14
Ubiquitin thioesterase 14
Ubiquitin-specific-processing protease 14
Gene namesi
Name:Usp14
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1928898. Usp14.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806371 – 493Ubiquitin carboxyl-terminal hydrolase 14Add BLAST493

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphothreonineCombined sources1
Modified residuei143PhosphoserineCombined sources1
Modified residuei148PhosphoserineCombined sources1
Modified residuei235PhosphothreonineBy similarity1
Modified residuei237PhosphoserineBy similarity1
Modified residuei302PhosphoserineBy similarity1
Modified residuei431PhosphoserineBy similarity1
Modified residuei448N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9JMA1.
MaxQBiQ9JMA1.
PaxDbiQ9JMA1.
PeptideAtlasiQ9JMA1.
PRIDEiQ9JMA1.

PTM databases

iPTMnetiQ9JMA1.
PhosphoSitePlusiQ9JMA1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000047879.
CleanExiMM_USP14.
ExpressionAtlasiQ9JMA1. baseline and differential.
GenevisibleiQ9JMA1. MM.

Interactioni

Subunit structurei

Homodimer (Potential). Interacts with FANCC, CXCR4 and ERN1 (By similarity). Associates with the 26S proteasome.By similarityCurated

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208492. 7 interactors.
IntActiQ9JMA1. 1 interactor.
MINTiMINT-4139172.
STRINGi10090.ENSMUSP00000089728.

Structurei

Secondary structure

1493
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 10Combined sources7
Beta strandi13 – 24Combined sources12
Helixi26 – 36Combined sources11
Turni41 – 43Combined sources3
Beta strandi68 – 70Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGGNMR-A4-86[»]
ProteinModelPortaliQ9JMA1.
SMRiQ9JMA1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JMA1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 80Ubiquitin-likePROSITE-ProRule annotationAdd BLAST77
Domaini105 – 482USPAdd BLAST378

Sequence similaritiesi

Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
HOVERGENiHBG054185.
InParanoidiQ9JMA1.
KOiK11843.
OMAiFKVKVKW.
OrthoDBiEOG091G06MR.
PhylomeDBiQ9JMA1.
TreeFamiTF314494.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JMA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG
60 70 80 90 100
GTLKDDDWGN IKMKNGMTVL MMGSADALPE EPSAKTVFVE DMTEEQLATA
110 120 130 140 150
MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ
160 170 180 190 200
YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA
210 220 230 240 250
NECWIQMMRV LQQKLEAIED DSGRETDSSS APAVTPSKKK SLIDQYFGVE
260 270 280 290 300
FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK
310 320 330 340 350
QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP
360 370 380 390 400
LMLDVYELCT PELQEKMVSF RSKFKDLEDK KVNQQPNAND KNSPPKEIKY
410 420 430 440 450
EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVR RKQDEWIKFD
460 470 480 490
DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE SEQ
Length:493
Mass (Da):56,002
Last modified:January 23, 2007 - v3
Checksum:i4E5F5DCB86057FF9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185Q → R in BAA93551 (Ref. 1) Curated1
Sequence conflicti310L → S in BAA93551 (Ref. 1) Curated1
Sequence conflicti385Q → E in BAB27544 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB034633 mRNA. Translation: BAA93551.1.
AK011322 mRNA. Translation: BAB27544.1.
AK029977 mRNA. Translation: BAC26713.1.
AK045909 mRNA. Translation: BAC32528.1.
BC005571 mRNA. Translation: AAH05571.1.
CCDSiCCDS37735.1.
RefSeqiNP_067497.2. NM_021522.4.
UniGeneiMm.329277.
Mm.447089.

Genome annotation databases

EnsembliENSMUST00000092096; ENSMUSP00000089728; ENSMUSG00000047879.
GeneIDi59025.
KEGGimmu:59025.
UCSCiuc008ean.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB034633 mRNA. Translation: BAA93551.1.
AK011322 mRNA. Translation: BAB27544.1.
AK029977 mRNA. Translation: BAC26713.1.
AK045909 mRNA. Translation: BAC32528.1.
BC005571 mRNA. Translation: AAH05571.1.
CCDSiCCDS37735.1.
RefSeqiNP_067497.2. NM_021522.4.
UniGeneiMm.329277.
Mm.447089.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WGGNMR-A4-86[»]
ProteinModelPortaliQ9JMA1.
SMRiQ9JMA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208492. 7 interactors.
IntActiQ9JMA1. 1 interactor.
MINTiMINT-4139172.
STRINGi10090.ENSMUSP00000089728.

Protein family/group databases

MEROPSiC19.015.

PTM databases

iPTMnetiQ9JMA1.
PhosphoSitePlusiQ9JMA1.

Proteomic databases

EPDiQ9JMA1.
MaxQBiQ9JMA1.
PaxDbiQ9JMA1.
PeptideAtlasiQ9JMA1.
PRIDEiQ9JMA1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000092096; ENSMUSP00000089728; ENSMUSG00000047879.
GeneIDi59025.
KEGGimmu:59025.
UCSCiuc008ean.1. mouse.

Organism-specific databases

CTDi9097.
MGIiMGI:1928898. Usp14.

Phylogenomic databases

eggNOGiKOG1872. Eukaryota.
ENOG410XP96. LUCA.
GeneTreeiENSGT00390000009615.
HOGENOMiHOG000202292.
HOVERGENiHBG054185.
InParanoidiQ9JMA1.
KOiK11843.
OMAiFKVKVKW.
OrthoDBiEOG091G06MR.
PhylomeDBiQ9JMA1.
TreeFamiTF314494.

Enzyme and pathway databases

ReactomeiR-MMU-5689880. Ub-specific processing proteases.

Miscellaneous databases

EvolutionaryTraceiQ9JMA1.
PROiQ9JMA1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047879.
CleanExiMM_USP14.
ExpressionAtlasiQ9JMA1. baseline and differential.
GenevisibleiQ9JMA1. MM.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
IPR000626. Ubiquitin_dom.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP14_MOUSE
AccessioniPrimary (citable) accession number: Q9JMA1
Secondary accession number(s): Q543U5, Q923F2, Q9D0L0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.