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Q9JMA1

- UBP14_MOUSE

UniProt

Q9JMA1 - UBP14_MOUSE

Protein

Ubiquitin carboxyl-terminal hydrolase 14

Gene

Usp14

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Maintains the cellular levels of monomeric ubiquitin in cells. Indispensable for synaptic development and function at neuromuscular junctions (NMJs). Is a reversibly associated subunit of the proteasome and a large fraction of proteasome-free protein exists within the cell. Required for the degradation of the chemokine receptor cxcr4 which is critical for CXCL12-induced cell chemotaxis. Serves also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1.2 Publications

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141NucleophilePROSITE-ProRule annotation
    Active sitei434 – 4341Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. endopeptidase inhibitor activity Source: Ensembl
    3. ubiquitin thiolesterase activity Source: Ensembl

    GO - Biological processi

    1. regulation of chemotaxis Source: Ensembl
    2. regulation of proteasomal protein catabolic process Source: Ensembl
    3. synaptic transmission Source: MGI
    4. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC19.015.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 14 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 14
    Ubiquitin thioesterase 14
    Ubiquitin-specific-processing protease 14
    Gene namesi
    Name:Usp14
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1928898. Usp14.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cell surface Source: Ensembl
    2. cytoplasmic membrane-bounded vesicle Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell
    4. proteasome complex Source: UniProtKB-KW
    5. synapse Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane, Proteasome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493Ubiquitin carboxyl-terminal hydrolase 14PRO_0000080637Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei143 – 1431Phosphoserine1 Publication
    Modified residuei235 – 2351PhosphothreonineBy similarity
    Modified residuei448 – 4481N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9JMA1.
    PaxDbiQ9JMA1.
    PRIDEiQ9JMA1.

    PTM databases

    PhosphoSiteiQ9JMA1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9JMA1.
    BgeeiQ9JMA1.
    CleanExiMM_USP14.
    GenevestigatoriQ9JMA1.

    Interactioni

    Subunit structurei

    Homodimer Potential. Interacts with FANCC, CXCR4 and ERN1 By similarity. Associates with the 26S proteasome.By similarityCurated

    Protein-protein interaction databases

    BioGridi208492. 5 interactions.
    IntActiQ9JMA1. 1 interaction.
    MINTiMINT-4139172.

    Structurei

    Secondary structure

    1
    493
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 107
    Beta strandi13 – 2412
    Helixi26 – 3611
    Turni41 – 433
    Beta strandi68 – 703

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1WGGNMR-A4-86[»]
    ProteinModelPortaliQ9JMA1.
    SMRiQ9JMA1. Positions 6-86, 98-482.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9JMA1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 8077Ubiquitin-likePROSITE-ProRule annotationAdd
    BLAST
    Domaini105 – 482378USPAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiNOG286607.
    GeneTreeiENSGT00390000009615.
    HOGENOMiHOG000202292.
    HOVERGENiHBG054185.
    KOiK11843.
    OMAiDWGNLKI.
    OrthoDBiEOG7F7W8H.
    PhylomeDBiQ9JMA1.
    TreeFamiTF314494.

    Family and domain databases

    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00213. UBQ. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    PROSITEiPS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JMA1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG    50
    GTLKDDDWGN IKMKNGMTVL MMGSADALPE EPSAKTVFVE DMTEEQLATA 100
    MELPCGLTNL GNTCYMNATV QCIRSVPELK DALKRYAGAL RASGEMASAQ 150
    YITAALRDLF DSMDKTSSSI PPIILLQFLH MAFPQFAEKG EQGQYLQQDA 200
    NECWIQMMRV LQQKLEAIED DSGRETDSSS APAVTPSKKK SLIDQYFGVE 250
    FETTMKCTES EEEEVTKGKE NQLQLSCFIN QEVKYLFTGL KLRLQEEITK 300
    QSPTLQRNAL YIKSSKISRL PAYLTIQMVR FFYKEKESVN AKVLKDVKFP 350
    LMLDVYELCT PELQEKMVSF RSKFKDLEDK KVNQQPNAND KNSPPKEIKY 400
    EPFSFADDIG SNNCGYYDLQ AVLTHQGRSS SSGHYVSWVR RKQDEWIKFD 450
    DDKVSIVTPE DILRLSGGGD WHIAYVLLYG PRRVEIMEEE SEQ 493
    Length:493
    Mass (Da):56,002
    Last modified:January 23, 2007 - v3
    Checksum:i4E5F5DCB86057FF9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti185 – 1851Q → R in BAA93551. 1 PublicationCurated
    Sequence conflicti310 – 3101L → S in BAA93551. 1 PublicationCurated
    Sequence conflicti385 – 3851Q → E in BAB27544. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB034633 mRNA. Translation: BAA93551.1.
    AK011322 mRNA. Translation: BAB27544.1.
    AK029977 mRNA. Translation: BAC26713.1.
    AK045909 mRNA. Translation: BAC32528.1.
    BC005571 mRNA. Translation: AAH05571.1.
    CCDSiCCDS37735.1.
    RefSeqiNP_067497.2. NM_021522.4.
    UniGeneiMm.329277.
    Mm.447089.

    Genome annotation databases

    EnsembliENSMUST00000092096; ENSMUSP00000089728; ENSMUSG00000047879.
    GeneIDi59025.
    KEGGimmu:59025.
    UCSCiuc008ean.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB034633 mRNA. Translation: BAA93551.1 .
    AK011322 mRNA. Translation: BAB27544.1 .
    AK029977 mRNA. Translation: BAC26713.1 .
    AK045909 mRNA. Translation: BAC32528.1 .
    BC005571 mRNA. Translation: AAH05571.1 .
    CCDSi CCDS37735.1.
    RefSeqi NP_067497.2. NM_021522.4.
    UniGenei Mm.329277.
    Mm.447089.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1WGG NMR - A 4-86 [» ]
    ProteinModelPortali Q9JMA1.
    SMRi Q9JMA1. Positions 6-86, 98-482.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208492. 5 interactions.
    IntActi Q9JMA1. 1 interaction.
    MINTi MINT-4139172.

    Protein family/group databases

    MEROPSi C19.015.

    PTM databases

    PhosphoSitei Q9JMA1.

    Proteomic databases

    MaxQBi Q9JMA1.
    PaxDbi Q9JMA1.
    PRIDEi Q9JMA1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000092096 ; ENSMUSP00000089728 ; ENSMUSG00000047879 .
    GeneIDi 59025.
    KEGGi mmu:59025.
    UCSCi uc008ean.1. mouse.

    Organism-specific databases

    CTDi 9097.
    MGIi MGI:1928898. Usp14.

    Phylogenomic databases

    eggNOGi NOG286607.
    GeneTreei ENSGT00390000009615.
    HOGENOMi HOG000202292.
    HOVERGENi HBG054185.
    KOi K11843.
    OMAi DWGNLKI.
    OrthoDBi EOG7F7W8H.
    PhylomeDBi Q9JMA1.
    TreeFami TF314494.

    Miscellaneous databases

    EvolutionaryTracei Q9JMA1.
    NextBioi 314596.
    PROi Q9JMA1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9JMA1.
    Bgeei Q9JMA1.
    CleanExi MM_USP14.
    Genevestigatori Q9JMA1.

    Family and domain databases

    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR000626. Ubiquitin-like.
    IPR029071. Ubiquitin-rel_dom.
    IPR019954. Ubiquitin_CS.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00213. UBQ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    PROSITEi PS00299. UBIQUITIN_1. 1 hit.
    PS50053. UBIQUITIN_2. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse deubiquitinating enzyme-type TGT."
      Hidaka T., Morishita T.
      Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Corpora quadrigemina, Embryo and Testis.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. "A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14."
      Borodovsky A., Kessler B.M., Casagrande R., Overkleeft H.S., Wilkinson K.D., Ploegh H.L.
      EMBO J. 20:5187-5196(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, ASSOCIATION WITH THE 26S PROTEASOME.
    5. "Loss of Usp14 results in reduced levels of ubiquitin in ataxia mice."
      Anderson C., Crimmins S., Wilson J.A., Korbel G.A., Ploegh H.L., Wilson S.M.
      J. Neurochem. 95:724-731(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ASSOCIATION WITH THE 26S PROTEASOME.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-143, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "The proteasome-associated deubiquitinating enzyme Usp14 is essential for the maintenance of synaptic ubiquitin levels and the development of neuromuscular junctions."
      Chen P.C., Qin L.N., Li X.M., Walters B.J., Wilson J.A., Mei L., Wilson S.M.
      J. Neurosci. 29:10909-10919(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Solution structure of the N-terminal ubiquitin-like domain of mouse ubiquitin specific protease 14 (usp14)."
      RIKEN structural genomics initiative (RSGI)
      Submitted (NOV-2004) to the PDB data bank
      Cited for: STRUCTURE BY NMR OF 4-86.

    Entry informationi

    Entry nameiUBP14_MOUSE
    AccessioniPrimary (citable) accession number: Q9JMA1
    Secondary accession number(s): Q543U5, Q923F2, Q9D0L0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 16, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3