Q9JM99 (PRG4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteoglycan 4

Alternative name(s):
Lubricin
Megakaryocyte-stimulating factor
Superficial zone proteoglycan

Cleaved into the following chain:

1. Proteoglycan 4 C-terminal part

Gene names

Name:	Prg4
Synonyms:	Msf, Szp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1054 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Ref.3
Subunit structure	Homodimer; disulfide-linked. Ref.4
Subcellular location	Secreted By similarity.
Tissue specificity	Highly expressed in cartilage, bone and liver and weakly expressed in heart, brain and muscle. Expressed in the surface chondrocytes and in synovial intimal cells. Isoform B is expressed in bone, small intestine, muscle, testis, heart, liver and lung. Isoform C and isoform D are widely expressed. Ref.1 Ref.3
Developmental stage	First detected at the forming joint surface from E15.5, after cavitation has begun. At later stages of morphogenesis, strong expression is observed in cartilage surface cells (superficial zone chondocytes) and in the newly forming synovium. Ref.3
Post-translational modification	N-glycosylated By similarity. O-glycosylated; contains glycosaminoglycan chondroitin sulfate and keratan sulfate By similarity. The disulfide bond between Cys-795 and Cys-1053 is essential for protein cleavage.
Disruption phenotype	Mice are viable and fertile. In the newborn period, their joints appear normal. The aged mice exhibit abnormal protein deposits on the cartilage surface and disappearance of underlying superficial zone chondrocytes. In addition to cartilage surface changes and subsequent cartilage deterioration, intimal cells in the synovium surrounding the joint space become hyperplastic, which further contribute to joint failure. Ref.3
Miscellaneous	Different forms varying in molecular weight have been observed. Such forms are possibly due to different levels of glycosylation and protein cleavage.
Sequence similarities	Contains 2 hemopexin-like domains. Contains 2 SMB (somatomedin-B) domains.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
PTM	Disulfide bond Glycoprotein Proteoglycan
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	hematopoietic stem cell proliferation Inferred from mutant phenotype PubMed 21939632. Source: MGI immune response Inferred from electronic annotation. Source: InterPro negative regulation of interleukin-6 biosynthetic process Inferred from mutant phenotype PubMed 21939632. Source: MGI regulation of cell proliferation Inferred from mutant phenotype Ref.3. Source: MGI
Cellular_component	extracellular space Inferred from direct assay Ref.3. Source: MGI
Molecular_function	polysaccharide binding Inferred from electronic annotation. Source: InterPro scavenger receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9JM99-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9JM99-2)

The sequence of this isoform differs from the canonical sequence as follows:
     26-66: Missing.

Isoform C (identifier: Q9JM99-3)

The sequence of this isoform differs from the canonical sequence as follows:
     107-194: Missing.

Isoform D (identifier: Q9JM99-4)

The sequence of this isoform differs from the canonical sequence as follows:
     26-66: Missing.
     107-194: Missing.

Isoform E (identifier: Q9JM99-5)

The sequence of this isoform differs from the canonical sequence as follows:
     107-194: Missing.
     224-766: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 1054	1030	Proteoglycan 4		PRO_0000043234
	Chain	956 – 1054	99	Proteoglycan 4 C-terminal part		PRO_0000043235
Regions
	Domain	26 – 69	44	SMB 1
	Domain	66 – 108	43	SMB 2
	Repeat	317 – 324	8	1; approximate
	Repeat	325 – 332	8	2; approximate
	Repeat	333 – 340	8	3; approximate
	Repeat	349 – 356	8	4; approximate
	Repeat	357 – 364	8	5
	Repeat	365 – 371	7	6; approximate
	Repeat	372 – 379	8	7
	Repeat	380 – 387	8	8
	Repeat	388 – 395	8	9
	Repeat	396 – 403	8	10
	Repeat	404 – 411	8	11
	Repeat	412 – 418	7	12; approximate
	Repeat	419 – 426	8	13
	Repeat	427 – 434	8	14
	Repeat	435 – 442	8	15
	Repeat	443 – 450	8	16; approximate
	Repeat	451 – 458	8	17
	Repeat	459 – 466	8	18
	Repeat	467 – 474	8	19
	Repeat	475 – 482	8	20
	Repeat	483 – 490	8	21
	Repeat	491 – 498	8	22
	Repeat	499 – 506	8	23
	Repeat	507 – 514	8	24
	Repeat	515 – 522	8	25
	Repeat	523 – 530	8	26
	Repeat	531 – 538	8	27
	Repeat	539 – 546	8	28
	Repeat	547 – 554	8	29
	Repeat	555 – 562	8	30
	Repeat	563 – 570	8	31
	Repeat	571 – 578	8	32
	Repeat	579 – 586	8	33
	Repeat	587 – 594	8	34
	Repeat	595 – 602	8	35
	Repeat	603 – 610	8	36
	Repeat	611 – 618	8	37
	Domain	800 – 842	43	Hemopexin-like 1
	Domain	844 – 890	47	Hemopexin-like 2
	Region	317 – 618	302	37 X 8 AA repeats of K-X-P-X-P-T-T-X
	Compositional bias	157 – 184	28	Ser-rich
	Compositional bias	350 – 606	257	Glu-rich
	Compositional bias	659 – 699	41	Lys-rich
Sites
	Site	955 – 956	2	Cleavage; by subtilisin-like proprotein convertase 4
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	938	1	N-linked (GlcNAc...) Potential
	Disulfide bond	30 ↔ 46		Alternate By similarity
	Disulfide bond	30 ↔ 34		By similarity
	Disulfide bond	34 ↔ 64		Alternate By similarity
	Disulfide bond	44 ↔ 57		Alternate By similarity
	Disulfide bond	44 ↔ 46		By similarity
	Disulfide bond	50 ↔ 56		By similarity
	Disulfide bond	57 ↔ 64		By similarity
	Disulfide bond	70 ↔ 86		Alternate By similarity
	Disulfide bond	70 ↔ 74		By similarity
	Disulfide bond	74 ↔ 104		Alternate By similarity
	Disulfide bond	84 ↔ 97		Alternate By similarity
	Disulfide bond	84 ↔ 86		By similarity
	Disulfide bond	90 ↔ 96		By similarity
	Disulfide bond	97 ↔ 104		By similarity
	Disulfide bond	795 ↔ 1053		Ref.4
Natural variations
	Alternative sequence	26 – 66	41	Missing in isoform B and isoform D.		VSP_016471
	Alternative sequence	107 – 194	88	Missing in isoform C, isoform D and isoform E.		VSP_016472
	Alternative sequence	224 – 766	543	Missing in isoform E.		VSP_016473
Experimental info
	Mutagenesis	795	1	C → A: Not cleaved by subtilisin-like proprotein convertase. Not cleaved by subtilisin-like proprotein convertase; when associated with A-1053. Ref.4
	Mutagenesis	851 – 853	3	CNC → ANA: Cleaved by subtilisin-like proprotein convertase. Ref.4
	Mutagenesis	851	1	C → A: Cleaved by subtilisin-like proprotein convertase.
	Mutagenesis	930	1	C → A: Cleaved by subtilisin-like proprotein convertase. Ref.4
	Mutagenesis	949 – 955	7	RRRRFER → ARARFEA: Not cleaved by subtilisin-like proprotein convertase 4. Ref.4
	Mutagenesis	949 – 951	3	RRR → ARA: Cleaved by subtilisin-like proprotein convertase 4. Ref.4
	Mutagenesis	955	1	R → A: Not cleaved by subtilisin-like proprotein convertase 4.
	Mutagenesis	1053	1	C → A: Not cleaved by subtilisin-like proprotein convertase 4. Not cleaved by subtilisin-like proprotein convertase; when associated with A-795. Ref.4
	Sequence conflict	65 – 66	2	SP → TT in BAE28900. Ref.2
	Sequence conflict	703	1	S → A in BAA92310. Ref.1
	Sequence conflict	780	1	Q → R in BAA92310. Ref.1
	Sequence conflict	788	1	L → P in BAA92310. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified December 6, 2005. Version 2. Checksum: 13312FB7070DF2A4	FASTA	1,054	115,996
	10 20 30 40 50 60 MGWKILPVCL SLLLPVVLIQ QVSSQDLSSC AGRCGEGYSR DATCNCDYNC QHYMECCPDF 70 80 90 100 110 120 KRVCSPELSC KGRCFESFAR GRECDCDSQC KQYGKCCADY DSFCEEVHNS TSPSSKTAPT 130 140 150 160 170 180 PAGASDTIKS TTKRSPKSPT TRTIKVVESE ELTEEHSDSE NQESSSSSSS SSSTIRKIKS 190 200 210 220 230 240 SKNSANRELQ KNPNVKDNKK NTPKKKPNPE PPAVDEAGSG LDNGEFKLTP PPPDPPTTPH 250 260 270 280 290 300 SKVATSPKTT AAKPVTPKPS LAPNSETSKE ASLASNKETT VETKETTATN KQSSASKKKT 310 320 330 340 350 360 TSVKETRSAE KTSDKDVEPT STTPKNSAPT TTKKPVTTTK ESKFLPLPQE PEPTTAKEPP 370 380 390 400 410 420 PTTKKPEPTT RKEPEPTTPK EPEPTTPKEP EPTTPKEPEP TTPKEPPPTT KKPEPTTPKE 430 440 450 460 470 480 PGPTTPKEPE PTTTKEPEPT TTKEPESTTR KEPEPTTPKE PEPTTPKEPE PTTLKEPEPT 490 500 510 520 530 540 TPKEPEPTTP KEPEPTTPKE PEPTTPKEPE PTTPKEPEPT TPKEPEPTTP KEPEPTTPKE 550 560 570 580 590 600 PEPTTPKKPE PTTPKEPVPT TPKEPEPTTP KEPEPTTPKE PEPTTRKEPE PTTPKEPEPT 610 620 630 640 650 660 TPKEPEPTTP KKPEPTTTSP KTTTLKATTL APKVTAPAEE IQNKPEETTP ASEDSDDSKT 670 680 690 700 710 720 TLKPQKPTKA PKPTKKPTKA PKKPTSTKKP KTPKTRKPKT TPSPLKTTSA TPELNTTPLE 730 740 750 760 770 780 VMLPTTTIPK QTPNPETAEV NPDHEDADGG EGEKPLIPGP PVLFPTAIPG TDLLAGRLNQ 790 800 810 820 830 840 GININPMLSD ETNLCNGKPV DGLTTLRNGT LVAFRGHYFW MLNPFRPPSP PRRITEVWGI 850 860 870 880 890 900 PSPIDTVFTR CNCEGKTFFF KDSQYWRFTN DVVDPGYPKQ IVKGFGGLTG KIVAALSIAK 910 920 930 940 950 960 YKDRPESVYF FKRGGNIQQY TYKQEPMKKC TGRRPAINYS VYGEAAQVRR RRFERAVGPF 970 980 990 1000 1010 1020 QTHTFRIHYS VPMRVSYQDK GFLHNEVKVS TMWRGFPNVV TSAITLPNIR KPDGYDYYAF 1030 1040 1050 SKDQYYNIDV PTRTARAITT RSGQTLSKIW YNCP
	Isoform B [UniParc]. Checksum: 49334633D98BD1E4	FASTA	1,013	111,425
	10 20 30 40 50 60 MGWKILPVCL SLLLPVVLIQ QVSSQELSCK GRCFESFARG RECDCDSQCK QYGKCCADYD 70 80 90 100 110 120 SFCEEVHNST SPSSKTAPTP AGASDTIKST TKRSPKSPTT RTIKVVESEE LTEEHSDSEN 130 140 150 160 170 180 QESSSSSSSS SSTIRKIKSS KNSANRELQK NPNVKDNKKN TPKKKPNPEP PAVDEAGSGL 190 200 210 220 230 240 DNGEFKLTPP PPDPPTTPHS KVATSPKTTA AKPVTPKPSL APNSETSKEA SLASNKETTV 250 260 270 280 290 300 ETKETTATNK QSSASKKKTT SVKETRSAEK TSDKDVEPTS TTPKNSAPTT TKKPVTTTKE 310 320 330 340 350 360 SKFLPLPQEP EPTTAKEPPP TTKKPEPTTR KEPEPTTPKE PEPTTPKEPE PTTPKEPEPT 370 380 390 400 410 420 TPKEPPPTTK KPEPTTPKEP GPTTPKEPEP TTTKEPEPTT TKEPESTTRK EPEPTTPKEP 430 440 450 460 470 480 EPTTPKEPEP TTLKEPEPTT PKEPEPTTPK EPEPTTPKEP EPTTPKEPEP TTPKEPEPTT 490 500 510 520 530 540 PKEPEPTTPK EPEPTTPKEP EPTTPKKPEP TTPKEPVPTT PKEPEPTTPK EPEPTTPKEP 550 560 570 580 590 600 EPTTRKEPEP TTPKEPEPTT PKEPEPTTPK KPEPTTTSPK TTTLKATTLA PKVTAPAEEI 610 620 630 640 650 660 QNKPEETTPA SEDSDDSKTT LKPQKPTKAP KPTKKPTKAP KKPTSTKKPK TPKTRKPKTT 670 680 690 700 710 720 PSPLKTTSAT PELNTTPLEV MLPTTTIPKQ TPNPETAEVN PDHEDADGGE GEKPLIPGPP 730 740 750 760 770 780 VLFPTAIPGT DLLAGRLNQG ININPMLSDE TNLCNGKPVD GLTTLRNGTL VAFRGHYFWM 790 800 810 820 830 840 LNPFRPPSPP RRITEVWGIP SPIDTVFTRC NCEGKTFFFK DSQYWRFTND VVDPGYPKQI 850 860 870 880 890 900 VKGFGGLTGK IVAALSIAKY KDRPESVYFF KRGGNIQQYT YKQEPMKKCT GRRPAINYSV 910 920 930 940 950 960 YGEAAQVRRR RFERAVGPFQ THTFRIHYSV PMRVSYQDKG FLHNEVKVST MWRGFPNVVT 970 980 990 1000 1010 SAITLPNIRK PDGYDYYAFS KDQYYNIDVP TRTARAITTR SGQTLSKIWY NCP
	Isoform C [UniParc]. Checksum: 123569A1FABFC5BD	FASTA	966	106,637
	10 20 30 40 50 60 MGWKILPVCL SLLLPVVLIQ QVSSQDLSSC AGRCGEGYSR DATCNCDYNC QHYMECCPDF 70 80 90 100 110 120 KRVCSPELSC KGRCFESFAR GRECDCDSQC KQYGKCCADY DSFCEEVKDN KKNTPKKKPN 130 140 150 160 170 180 PEPPAVDEAG SGLDNGEFKL TPPPPDPPTT PHSKVATSPK TTAAKPVTPK PSLAPNSETS 190 200 210 220 230 240 KEASLASNKE TTVETKETTA TNKQSSASKK KTTSVKETRS AEKTSDKDVE PTSTTPKNSA 250 260 270 280 290 300 PTTTKKPVTT TKESKFLPLP QEPEPTTAKE PPPTTKKPEP TTRKEPEPTT PKEPEPTTPK 310 320 330 340 350 360 EPEPTTPKEP EPTTPKEPPP TTKKPEPTTP KEPGPTTPKE PEPTTTKEPE PTTTKEPEST 370 380 390 400 410 420 TRKEPEPTTP KEPEPTTPKE PEPTTLKEPE PTTPKEPEPT TPKEPEPTTP KEPEPTTPKE 430 440 450 460 470 480 PEPTTPKEPE PTTPKEPEPT TPKEPEPTTP KEPEPTTPKK PEPTTPKEPV PTTPKEPEPT 490 500 510 520 530 540 TPKEPEPTTP KEPEPTTRKE PEPTTPKEPE PTTPKEPEPT TPKKPEPTTT SPKTTTLKAT 550 560 570 580 590 600 TLAPKVTAPA EEIQNKPEET TPASEDSDDS KTTLKPQKPT KAPKPTKKPT KAPKKPTSTK 610 620 630 640 650 660 KPKTPKTRKP KTTPSPLKTT SATPELNTTP LEVMLPTTTI PKQTPNPETA EVNPDHEDAD 670 680 690 700 710 720 GGEGEKPLIP GPPVLFPTAI PGTDLLAGRL NQGININPML SDETNLCNGK PVDGLTTLRN 730 740 750 760 770 780 GTLVAFRGHY FWMLNPFRPP SPPRRITEVW GIPSPIDTVF TRCNCEGKTF FFKDSQYWRF 790 800 810 820 830 840 TNDVVDPGYP KQIVKGFGGL TGKIVAALSI AKYKDRPESV YFFKRGGNIQ QYTYKQEPMK 850 860 870 880 890 900 KCTGRRPAIN YSVYGEAAQV RRRRFERAVG PFQTHTFRIH YSVPMRVSYQ DKGFLHNEVK 910 920 930 940 950 960 VSTMWRGFPN VVTSAITLPN IRKPDGYDYY AFSKDQYYNI DVPTRTARAI TTRSGQTLSK IWYNCP
	Isoform D [UniParc]. Checksum: 1CD5556F5467CD48	FASTA	925	102,066
	10 20 30 40 50 60 MGWKILPVCL SLLLPVVLIQ QVSSQELSCK GRCFESFARG RECDCDSQCK QYGKCCADYD 70 80 90 100 110 120 SFCEEVKDNK KNTPKKKPNP EPPAVDEAGS GLDNGEFKLT PPPPDPPTTP HSKVATSPKT 130 140 150 160 170 180 TAAKPVTPKP SLAPNSETSK EASLASNKET TVETKETTAT NKQSSASKKK TTSVKETRSA 190 200 210 220 230 240 EKTSDKDVEP TSTTPKNSAP TTTKKPVTTT KESKFLPLPQ EPEPTTAKEP PPTTKKPEPT 250 260 270 280 290 300 TRKEPEPTTP KEPEPTTPKE PEPTTPKEPE PTTPKEPPPT TKKPEPTTPK EPGPTTPKEP 310 320 330 340 350 360 EPTTTKEPEP TTTKEPESTT RKEPEPTTPK EPEPTTPKEP EPTTLKEPEP TTPKEPEPTT 370 380 390 400 410 420 PKEPEPTTPK EPEPTTPKEP EPTTPKEPEP TTPKEPEPTT PKEPEPTTPK EPEPTTPKKP 430 440 450 460 470 480 EPTTPKEPVP TTPKEPEPTT PKEPEPTTPK EPEPTTRKEP EPTTPKEPEP TTPKEPEPTT 490 500 510 520 530 540 PKKPEPTTTS PKTTTLKATT LAPKVTAPAE EIQNKPEETT PASEDSDDSK TTLKPQKPTK 550 560 570 580 590 600 APKPTKKPTK APKKPTSTKK PKTPKTRKPK TTPSPLKTTS ATPELNTTPL EVMLPTTTIP 610 620 630 640 650 660 KQTPNPETAE VNPDHEDADG GEGEKPLIPG PPVLFPTAIP GTDLLAGRLN QGININPMLS 670 680 690 700 710 720 DETNLCNGKP VDGLTTLRNG TLVAFRGHYF WMLNPFRPPS PPRRITEVWG IPSPIDTVFT 730 740 750 760 770 780 RCNCEGKTFF FKDSQYWRFT NDVVDPGYPK QIVKGFGGLT GKIVAALSIA KYKDRPESVY 790 800 810 820 830 840 FFKRGGNIQQ YTYKQEPMKK CTGRRPAINY SVYGEAAQVR RRRFERAVGP FQTHTFRIHY 850 860 870 880 890 900 SVPMRVSYQD KGFLHNEVKV STMWRGFPNV VTSAITLPNI RKPDGYDYYA FSKDQYYNID 910 920 VPTRTARAIT TRSGQTLSKI WYNCP
	Isoform E [UniParc]. Checksum: EBC6B86287506E4C	FASTA	423	47,989
	10 20 30 40 50 60 MGWKILPVCL SLLLPVVLIQ QVSSQDLSSC AGRCGEGYSR DATCNCDYNC QHYMECCPDF 70 80 90 100 110 120 KRVCSPELSC KGRCFESFAR GRECDCDSQC KQYGKCCADY DSFCEEVKDN KKNTPKKKPN 130 140 150 160 170 180 PEPPAVDEAG SGLDNAIPGT DLLAGRLNQG ININPMLSDE TNLCNGKPVD GLTTLRNGTL 190 200 210 220 230 240 VAFRGHYFWM LNPFRPPSPP RRITEVWGIP SPIDTVFTRC NCEGKTFFFK DSQYWRFTND 250 260 270 280 290 300 VVDPGYPKQI VKGFGGLTGK IVAALSIAKY KDRPESVYFF KRGGNIQQYT YKQEPMKKCT 310 320 330 340 350 360 GRRPAINYSV YGEAAQVRRR RFERAVGPFQ THTFRIHYSV PMRVSYQDKG FLHNEVKVST 370 380 390 400 410 420 MWRGFPNVVT SAITLPNIRK PDGYDYYAFS KDQYYNIDVP TRTARAITTR SGQTLSKIWY NCP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, characterization and mapping of the mouse and human PRG4 (proteoglycan 4) genes." Ikegawa S., Sano M., Koshizuka Y., Nakamura Y. Cytogenet. Cell Genet. 90:291-297(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, IDENTIFICATION OF ISOFORMS B; C AND D.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM E), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 572-1054. Strain: C57BL/6J. Tissue: Head and Liver.
[3]	"The secreted glycoprotein lubricin protects cartilage surfaces and inhibits synovial cell overgrowth." Rhee D.K., Marcelino J., Baker M., Gong Y., Smits P., Lefebvre V., Jay G.D., Stewart M., Wang H., Warman M.L., Carpten J.D. J. Clin. Invest. 115:622-631(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[4]	"Consequences of disease-causing mutations on lubricin protein synthesis, secretion, and post-translational processing." Rhee D.K., Marcelino J., Al-Mayouf S., Schelling D.K., Bartels C.F., Cui Y., Laxer R., Goldbach-Mansky R., Warman M.L. J. Biol. Chem. 280:31325-31332(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING, DISULFIDE BOND, MUTAGENESIS OF 949-ARG--ARG-955; 949-ARG--ARG-951; CYS-795; 851-CYS--CYS-853; CYS-930 AND CYS-1053.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB034730 mRNA. Translation: BAA92310.1. AK132597 mRNA. Translation: BAE21253.1. AK149469 mRNA. Translation: BAE28900.1.
IPI	IPI00652394. IPI00656174. IPI00656236. IPI00656272. IPI00656304.
RefSeq	NP_001103616.1. NM_001110146.1.
UniGene	Mm.174256. Mm.329131.
3D structure databases
ProteinModelPortal	Q9JM99.
SMR	Q9JM99. Positions 26-105, 793-929.
ModBase	Search...
PTM databases
PhosphoSite	Q9JM99.
Proteomic databases
PaxDb	Q9JM99.
PRIDE	Q9JM99.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000006171; ENSMUSP00000006171; ENSMUSG00000006014. ENSMUST00000162367; ENSMUSP00000125551; ENSMUSG00000006014.
GeneID	96875.
KEGG	mmu:96875.
Organism-specific databases
CTD	10216.
MGI	MGI:1891344. Prg4.
Phylogenomic databases
eggNOG	NOG12793.
GeneTree	ENSGT00530000063751.
HOGENOM	HOG000115691.
HOVERGEN	HBG101613.
InParanoid	Q9JM99.
OrthoDB	EOG4JM7QM.
Gene expression databases
ArrayExpress	Q9JM99.
Bgee	Q9JM99.
Genevestigator	Q9JM99.
GermOnline	ENSMUSG00000006014. Mus musculus.
Family and domain databases
Gene3D	2.110.10.10. 2 hits.
InterPro	IPR000585. Hemopexin-like_dom. IPR018487. Hemopexin-like_repeat. IPR018486. Hemopexin_CS. IPR020436. Somatomedin_B_chordata. IPR001212. Somatomedin_B_dom. [Graphical view]
Pfam	PF00045. Hemopexin. 2 hits. PF01033. Somatomedin_B. 2 hits. [Graphical view]
PRINTS	PR00022. SOMATOMEDINB.
SMART	SM00120. HX. 2 hits. SM00201. SO. 2 hits. [Graphical view]
SUPFAM	SSF50923. Hemopexin. 1 hit.
PROSITE	PS00024. HEMOPEXIN. 1 hit. PS00524. SMB_1. 2 hits. PS50958. SMB_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	352339.
SOURCE	Search...

Entry information

Entry name

PRG4_MOUSE

Accession

Primary (citable) accession number: Q9JM99
Secondary accession number(s): Q3UEL1, Q3V198

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 6, 2005
Last sequence update:	December 6, 2005
Last modified:	May 1, 2013
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program