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Protein

Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6

Gene

St6galnac6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no activity toward glycoproteins. Responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in kidney.1 Publication

GO - Molecular functioni

  • alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity Source: MGI
  • sialyltransferase activity Source: BHF-UCL

GO - Biological processi

  • cell-cell recognition Source: BHF-UCL
  • ganglioside biosynthetic process Source: MGI
  • glycoprotein metabolic process Source: MGI
  • glycosphingolipid metabolic process Source: MGI
  • glycosylceramide metabolic process Source: MGI
  • oligosaccharide biosynthetic process Source: BHF-UCL
  • protein N-linked glycosylation via asparagine Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Chemistry databases

SwissLipidsiSLP:000000778.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (EC:2.4.99.-)
Alternative name(s):
GalNAc alpha-2,6-sialyltransferase VI
ST6GalNAc VI
Short name:
ST6GalNAcVI
Sialyltransferase 7F
Short name:
SIAT7-F
Gene namesi
Name:St6galnac6
Synonyms:Siat7f
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1355316. St6galnac6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 43CytoplasmicSequence analysisAdd BLAST43
Transmembranei44 – 64Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini65 – 333LumenalSequence analysisAdd BLAST269

GO - Cellular componenti

  • cytoplasm Source: MGI
  • Golgi membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003147961 – 333Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6Add BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi108 ↔ 256By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9JM95.
PRIDEiQ9JM95.

PTM databases

iPTMnetiQ9JM95.
PhosphoSitePlusiQ9JM95.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Inductioni

After inflammation stimulus.1 Publication

Gene expression databases

BgeeiENSMUSG00000026811.
ExpressionAtlasiQ9JM95. baseline and differential.
GenevisibleiQ9JM95. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071983.

Structurei

3D structure databases

ProteinModelPortaliQ9JM95.
SMRiQ9JM95.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi30 – 33Poly-Arg4

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOVERGENiHBG058710.
InParanoidiQ9JM95.
KOiK03376.
OMAiDYCSQRP.
OrthoDBiEOG091G0H9S.
PhylomeDBiQ9JM95.
TreeFamiTF323961.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JM95-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MACSRPPSQC DPTTLPPGPP AGRWPLPFSR RRREMSSNKE QRSAVFVILF
60 70 80 90 100
ALITILILYS SNSANEVFHY GSLRGRTRRP VNLKKWSFSS AYFPILGNKT
110 120 130 140 150
LPSRCNQCVI ITSSSHLLGT KLGPEIERAE CTIRMNDAPT SGYSADVGNK
160 170 180 190 200
TTFRVVAHSS VFRVLRKPQE FVNRTPETVF IFWGPPNKMQ KPQGSLLRVI
210 220 230 240 250
QRAGLMFPNM EAYAVSPARM QQFDDLFRGE TGKDREKSHS WLSTGWFTMV
260 270 280 290 300
IAVELCDHVH VYGMVPPDYC SQRPRLQRMP YHYYEPKGPD ECVTYIQNEH
310 320 330
SRKGNHHRFI TEKRVFSSWA QLYGITFSHP SWT
Length:333
Mass (Da):38,167
Last modified:October 1, 2000 - v1
Checksum:iD3841828D389CDEA
GO
Isoform 2 (identifier: Q9JM95-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-9: MACSRPPSQ → MAQGNHEAWGW

Show »
Length:335
Mass (Da):38,477
Checksum:iB0D1B9E878501D78
GO
Isoform 3 (identifier: Q9JM95-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     230-235: ETGKDR → KSPIPG
     236-333: Missing.

Note: No experimental confirmation.
Show »
Length:235
Mass (Da):26,377
Checksum:i5F0B7183FCCEA3AC
GO

Sequence cautioni

The sequence CAM16604 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16608 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16609 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16610 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAM16611 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti103S → A in AAH22924 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0303601 – 9MACSRPPSQ → MAQGNHEAWGW in isoform 2. 1 Publication9
Alternative sequenceiVSP_030361230 – 235ETGKDR → KSPIPG in isoform 3. 1 Publication6
Alternative sequenceiVSP_030362236 – 333Missing in isoform 3. 1 PublicationAdd BLAST98

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035123 mRNA. Translation: BAA95940.1.
AB035174 mRNA. Translation: BAA87036.1.
AK030648 mRNA. Translation: BAC27064.1.
AL772271 Genomic DNA. Translation: CAM16604.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16606.1.
AL772271 Genomic DNA. Translation: CAM16607.1.
AL772271 Genomic DNA. Translation: CAM16608.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16609.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16610.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16611.1. Sequence problems.
BC022924 mRNA. Translation: AAH22924.1.
CCDSiCCDS15923.1. [Q9JM95-1]
RefSeqiNP_001020481.1. NM_001025310.2.
NP_001020482.1. NM_001025311.2.
NP_001276476.1. NM_001289547.1.
NP_001276477.1. NM_001289548.1.
NP_001276478.1. NM_001289549.1.
NP_058669.1. NM_016973.3. [Q9JM95-1]
UniGeneiMm.88831.

Genome annotation databases

EnsembliENSMUST00000072111; ENSMUSP00000071983; ENSMUSG00000026811. [Q9JM95-1]
ENSMUST00000081879; ENSMUSP00000080555; ENSMUSG00000026811. [Q9JM95-2]
ENSMUST00000095044; ENSMUSP00000092654; ENSMUSG00000026811. [Q9JM95-1]
ENSMUST00000183538; ENSMUSP00000138916; ENSMUSG00000026811. [Q9JM95-3]
GeneIDi50935.
KEGGimmu:50935.
UCSCiuc008jgb.2. mouse. [Q9JM95-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB035123 mRNA. Translation: BAA95940.1.
AB035174 mRNA. Translation: BAA87036.1.
AK030648 mRNA. Translation: BAC27064.1.
AL772271 Genomic DNA. Translation: CAM16604.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16606.1.
AL772271 Genomic DNA. Translation: CAM16607.1.
AL772271 Genomic DNA. Translation: CAM16608.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16609.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16610.1. Sequence problems.
AL772271 Genomic DNA. Translation: CAM16611.1. Sequence problems.
BC022924 mRNA. Translation: AAH22924.1.
CCDSiCCDS15923.1. [Q9JM95-1]
RefSeqiNP_001020481.1. NM_001025310.2.
NP_001020482.1. NM_001025311.2.
NP_001276476.1. NM_001289547.1.
NP_001276477.1. NM_001289548.1.
NP_001276478.1. NM_001289549.1.
NP_058669.1. NM_016973.3. [Q9JM95-1]
UniGeneiMm.88831.

3D structure databases

ProteinModelPortaliQ9JM95.
SMRiQ9JM95.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000071983.

Chemistry databases

SwissLipidsiSLP:000000778.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

iPTMnetiQ9JM95.
PhosphoSitePlusiQ9JM95.

Proteomic databases

PaxDbiQ9JM95.
PRIDEiQ9JM95.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072111; ENSMUSP00000071983; ENSMUSG00000026811. [Q9JM95-1]
ENSMUST00000081879; ENSMUSP00000080555; ENSMUSG00000026811. [Q9JM95-2]
ENSMUST00000095044; ENSMUSP00000092654; ENSMUSG00000026811. [Q9JM95-1]
ENSMUST00000183538; ENSMUSP00000138916; ENSMUSG00000026811. [Q9JM95-3]
GeneIDi50935.
KEGGimmu:50935.
UCSCiuc008jgb.2. mouse. [Q9JM95-1]

Organism-specific databases

CTDi30815.
MGIiMGI:1355316. St6galnac6.

Phylogenomic databases

eggNOGiKOG2692. Eukaryota.
ENOG410XT8P. LUCA.
GeneTreeiENSGT00550000074444.
HOVERGENiHBG058710.
InParanoidiQ9JM95.
KOiK03376.
OMAiDYCSQRP.
OrthoDBiEOG091G0H9S.
PhylomeDBiQ9JM95.
TreeFamiTF323961.

Enzyme and pathway databases

ReactomeiR-MMU-4085001. Sialic acid metabolism.

Miscellaneous databases

PROiQ9JM95.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026811.
ExpressionAtlasiQ9JM95. baseline and differential.
GenevisibleiQ9JM95. MM.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSIA7F_MOUSE
AccessioniPrimary (citable) accession number: Q9JM95
Secondary accession number(s): A2AK72
, A2AK76, A2AK77, A2AK78, A2AK79, Q05CN1, Q8CDC3, Q9R0G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.