Q9JM90 (STAP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal-transducing adaptor protein 1
Short name=STAP-1

Alternative name(s):
Stem cell adaptor protein 1

Gene names

Name:

Stap1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as an adapter molecule downstream of KIT in the proliferation or differentiation of hematopoietic stem cells. Ref.1
Subunit structure	Interacts with URI1; the interaction is phosphorylation-dependent occurs in a growth-dependent manner By similarity. Interacts with KIT and CSF1R. Ref.1
Subcellular location	Nucleus By similarity. Cytoplasm Probable. Mitochondrion By similarity.
Tissue specificity	Expression restricted to the bone marrow. Ref.1
Post-translational modification	Phosphorylated on tyrosine by TEC By similarity. Phosphorylated on tyrosine by KIT By similarity. Ref.1
Sequence similarities	Contains 1 PH domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion Nucleus
Coding sequence diversity	Alternative splicing
Domain	SH2 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	myeloid cell differentiation Traceable author statement Ref.1. Source: MGI transmembrane receptor protein tyrosine kinase signaling pathway Inferred from direct assay Ref.1. Source: MGI
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell protein complex Inferred from sequence orthology PubMed 18468998. Source: MGI
Molecular_function	SH3/SH2 adaptor activity Inferred from direct assay Ref.1. Source: MGI phospholipid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9JM90-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9JM90-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-127: LTVPQH → VIRFPL
     128-297: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Signal-transducing adaptor protein 1		PRO_0000072238
Regions
	Domain	25 – 121	97	PH
	Domain	179 – 273	95	SH2
Amino acid modifications
	Modified residue	170	1	Phosphotyrosine Ref.4
Natural variations
	Alternative sequence	122 – 127	6	LTVPQH → VIRFPL in isoform 2.		VSP_013399
	Alternative sequence	128 – 297	170	Missing in isoform 2.		VSP_013400

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8033C606990AAA75	FASTA	297	34,628
	10 20 30 40 50 60 MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLVKRSDHQ EYKHYWTELR GTTLFFYTDK 70 80 90 100 110 120 KSTIYVGKLD IIDLVCLTGQ HSTEKNCAKF TLVLPKEEVH VKTENTESGE EWRGFILTVT 130 140 150 160 170 180 ELTVPQHVSL LPGQVIRLHE VLEREKKRRI ETDQLPLMPP EKEKEPVQDY ADVLNPLPEC 190 200 210 220 230 240 FYAVSRKEAT AMLEKNPSWG NMILRPGSDS KNYSITIRQE IEMPRIKHFK VTRTGNNYTI 250 260 270 280 290 ELEKPVTLPN LFSVIDYFVK ETRGNLRPFI HSADDNFGQD PNIEDRSEKF KKNPHNA
	Isoform 2 [UniParc]. Checksum: AFAAE1584FA79F68	FASTA	127	14,921
	10 20 30 40 50 60 MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLVKRSDHQ EYKHYWTELR GTTLFFYTDK 70 80 90 100 110 120 KSTIYVGKLD IIDLVCLTGQ HSTEKNCAKF TLVLPKEEVH VKTENTESGE EWRGFILTVT EVIRFPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains." Masuhara M., Nagao K., Nishikawa M., Sasaki M., Yoshimura A., Osawa M. Biochem. Biophys. Res. Commun. 268:697-703(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, INTERACTION WITH KIT AND CSF1R, TISSUE SPECIFICITY.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Bone and Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: FVB/N. Tissue: Brain and Mammary gland.
[4]	"Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-170, MASS SPECTROMETRY. Tissue: Mast cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB036058 mRNA. Translation: BAA92531.1. AK041474 mRNA. Translation: BAC30953.1. AK162655 mRNA. Translation: BAE37007.1. BC057898 mRNA. Translation: AAH57898.1. BC145828 mRNA. Translation: AAI45829.1. BC145830 mRNA. Translation: AAI45831.1.
IPI	IPI00124897. IPI00554951.
RefSeq	NP_064376.1. NM_019992.3.
UniGene	Mm.131237.
3D structure databases
ProteinModelPortal	Q9JM90.
SMR	Q9JM90. Positions 16-152, 173-270.
ModBase	Search...
PTM databases
PhosphoSite	Q9JM90.
Proteomic databases
PaxDb	Q9JM90.
PRIDE	Q9JM90.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000031171; ENSMUSP00000031171; ENSMUSG00000029254.
GeneID	56792.
KEGG	mmu:56792.
UCSC	uc008xxf.1. mouse. uc008xxg.1. mouse.
Organism-specific databases
CTD	26228.
MGI	MGI:1926193. Stap1.
Phylogenomic databases
eggNOG	NOG47666.
GeneTree	ENSGT00530000063841.
HOGENOM	HOG000234375.
HOVERGEN	HBG062262.
InParanoid	A6H6C6.
OMA	NMILRPG.
OrthoDB	EOG4THVTM.
Gene expression databases
Bgee	Q9JM90.
CleanEx	MM_STAP1.
Genevestigator	Q9JM90.
GermOnline	ENSMUSG00000029254. Mus musculus.
Family and domain databases
Gene3D	2.30.29.30. 1 hit. 3.30.505.10. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000980. SH2. [Graphical view]
Pfam	PF00169. PH. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00252. SH2. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	313336.
SOURCE	Search...

Entry information

Entry name

STAP1_MOUSE

Accession

Primary (citable) accession number: Q9JM90
Secondary accession number(s): A6H6C6, Q3TRM1, Q6PES6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program