Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9JM90

- STAP1_MOUSE

UniProt

Q9JM90 - STAP1_MOUSE

Protein

Signal-transducing adaptor protein 1

Gene

Stap1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    May function as an adapter molecule downstream of KIT in the proliferation or differentiation of hematopoietic stem cells.1 Publication

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. SH3/SH2 adaptor activity Source: MGI

    GO - Biological processi

    1. myeloid cell differentiation Source: MGI
    2. positive regulation of signal transduction Source: GOC
    3. transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal-transducing adaptor protein 1
    Short name:
    STAP-1
    Alternative name(s):
    Stem cell adaptor protein 1
    Gene namesi
    Name:Stap1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1926193. Stap1.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm Curated. Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. protein complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Signal-transducing adaptor protein 1PRO_0000072238Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei170 – 1701Phosphotyrosine2 Publications

    Post-translational modificationi

    Phosphorylated on tyrosine by TEC. Phosphorylated on tyrosine by KIT.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9JM90.
    PaxDbiQ9JM90.
    PRIDEiQ9JM90.

    PTM databases

    PhosphoSiteiQ9JM90.

    Expressioni

    Tissue specificityi

    Expression restricted to the bone marrow.1 Publication

    Gene expression databases

    BgeeiQ9JM90.
    CleanExiMM_STAP1.
    GenevestigatoriQ9JM90.

    Interactioni

    Subunit structurei

    Interacts with URI1; the interaction is phosphorylation-dependent occurs in a growth-dependent manner By similarity. Interacts with KIT and CSF1R.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi208177. 4 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JM90.
    SMRiQ9JM90. Positions 16-152, 173-270.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 12197PHPROSITE-ProRule annotationAdd
    BLAST
    Domaini179 – 27395SH2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 PH domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain

    Phylogenomic databases

    eggNOGiNOG47666.
    GeneTreeiENSGT00530000063841.
    HOGENOMiHOG000234375.
    HOVERGENiHBG062262.
    InParanoidiA6H6C6.
    OMAiPMPACFY.
    OrthoDBiEOG79KPFP.
    PhylomeDBiQ9JM90.
    TreeFamiTF332087.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000980. SH2.
    [Graphical view]
    PfamiPF00169. PH. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    SM00252. SH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55550. SSF55550. 1 hit.
    PROSITEiPS50003. PH_DOMAIN. 1 hit.
    PS50001. SH2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9JM90-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLVKRSDHQ EYKHYWTELR    50
    GTTLFFYTDK KSTIYVGKLD IIDLVCLTGQ HSTEKNCAKF TLVLPKEEVH 100
    VKTENTESGE EWRGFILTVT ELTVPQHVSL LPGQVIRLHE VLEREKKRRI 150
    ETDQLPLMPP EKEKEPVQDY ADVLNPLPEC FYAVSRKEAT AMLEKNPSWG 200
    NMILRPGSDS KNYSITIRQE IEMPRIKHFK VTRTGNNYTI ELEKPVTLPN 250
    LFSVIDYFVK ETRGNLRPFI HSADDNFGQD PNIEDRSEKF KKNPHNA 297
    Length:297
    Mass (Da):34,628
    Last modified:October 1, 2000 - v1
    Checksum:i8033C606990AAA75
    GO
    Isoform 2 (identifier: Q9JM90-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         122-127: LTVPQH → VIRFPL
         128-297: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:127
    Mass (Da):14,921
    Checksum:iAFAAE1584FA79F68
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei122 – 1276LTVPQH → VIRFPL in isoform 2. 1 PublicationVSP_013399
    Alternative sequencei128 – 297170Missing in isoform 2. 1 PublicationVSP_013400Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB036058 mRNA. Translation: BAA92531.1.
    AK041474 mRNA. Translation: BAC30953.1.
    AK162655 mRNA. Translation: BAE37007.1.
    BC057898 mRNA. Translation: AAH57898.1.
    BC145828 mRNA. Translation: AAI45829.1.
    BC145830 mRNA. Translation: AAI45831.1.
    CCDSiCCDS19377.1. [Q9JM90-1]
    RefSeqiNP_064376.1. NM_019992.3. [Q9JM90-1]
    XP_006535224.1. XM_006535161.1. [Q9JM90-2]
    UniGeneiMm.131237.

    Genome annotation databases

    EnsembliENSMUST00000031171; ENSMUSP00000031171; ENSMUSG00000029254. [Q9JM90-1]
    GeneIDi56792.
    KEGGimmu:56792.
    UCSCiuc008xxf.1. mouse. [Q9JM90-2]
    uc008xxg.1. mouse. [Q9JM90-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB036058 mRNA. Translation: BAA92531.1 .
    AK041474 mRNA. Translation: BAC30953.1 .
    AK162655 mRNA. Translation: BAE37007.1 .
    BC057898 mRNA. Translation: AAH57898.1 .
    BC145828 mRNA. Translation: AAI45829.1 .
    BC145830 mRNA. Translation: AAI45831.1 .
    CCDSi CCDS19377.1. [Q9JM90-1 ]
    RefSeqi NP_064376.1. NM_019992.3. [Q9JM90-1 ]
    XP_006535224.1. XM_006535161.1. [Q9JM90-2 ]
    UniGenei Mm.131237.

    3D structure databases

    ProteinModelPortali Q9JM90.
    SMRi Q9JM90. Positions 16-152, 173-270.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 208177. 4 interactions.

    PTM databases

    PhosphoSitei Q9JM90.

    Proteomic databases

    MaxQBi Q9JM90.
    PaxDbi Q9JM90.
    PRIDEi Q9JM90.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031171 ; ENSMUSP00000031171 ; ENSMUSG00000029254 . [Q9JM90-1 ]
    GeneIDi 56792.
    KEGGi mmu:56792.
    UCSCi uc008xxf.1. mouse. [Q9JM90-2 ]
    uc008xxg.1. mouse. [Q9JM90-1 ]

    Organism-specific databases

    CTDi 26228.
    MGIi MGI:1926193. Stap1.

    Phylogenomic databases

    eggNOGi NOG47666.
    GeneTreei ENSGT00530000063841.
    HOGENOMi HOG000234375.
    HOVERGENi HBG062262.
    InParanoidi A6H6C6.
    OMAi PMPACFY.
    OrthoDBi EOG79KPFP.
    PhylomeDBi Q9JM90.
    TreeFami TF332087.

    Miscellaneous databases

    NextBioi 313336.
    PROi Q9JM90.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JM90.
    CleanExi MM_STAP1.
    Genevestigatori Q9JM90.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    IPR000980. SH2.
    [Graphical view ]
    Pfami PF00169. PH. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    SM00252. SH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55550. SSF55550. 1 hit.
    PROSITEi PS50003. PH_DOMAIN. 1 hit.
    PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains."
      Masuhara M., Nagao K., Nishikawa M., Sasaki M., Yoshimura A., Osawa M.
      Biochem. Biophys. Res. Commun. 268:697-703(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, INTERACTION WITH KIT AND CSF1R, TISSUE SPECIFICITY.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6J.
      Tissue: Bone and Thymus.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: FVB/N.
      Tissue: Brain and Mammary gland.
    4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
      Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
      J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Mast cell.

    Entry informationi

    Entry nameiSTAP1_MOUSE
    AccessioniPrimary (citable) accession number: Q9JM90
    Secondary accession number(s): A6H6C6, Q3TRM1, Q6PES6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 12, 2005
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3