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Protein

Signal-transducing adaptor protein 1

Gene

Stap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as an adapter molecule downstream of KIT in the proliferation or differentiation of hematopoietic stem cells.1 Publication

GO - Molecular functioni

  1. SH3/SH2 adaptor activity Source: MGI

GO - Biological processi

  1. myeloid cell differentiation Source: MGI
  2. positive regulation of signal transduction Source: GOC
  3. transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Signal-transducing adaptor protein 1
Short name:
STAP-1
Alternative name(s):
Stem cell adaptor protein 1
Gene namesi
Name:Stap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:1926193. Stap1.

Subcellular locationi

Nucleus By similarity. Cytoplasm Curated. Mitochondrion By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. mitochondrion Source: MGI
  3. nucleus Source: MGI
  4. protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Signal-transducing adaptor protein 1PRO_0000072238Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei170 – 1701Phosphotyrosine1 Publication

Post-translational modificationi

Phosphorylated on tyrosine by TEC. Phosphorylated on tyrosine by KIT.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JM90.
PaxDbiQ9JM90.
PRIDEiQ9JM90.

PTM databases

PhosphoSiteiQ9JM90.

Expressioni

Tissue specificityi

Expression restricted to the bone marrow.1 Publication

Gene expression databases

BgeeiQ9JM90.
CleanExiMM_STAP1.
GenevestigatoriQ9JM90.

Interactioni

Subunit structurei

Interacts with URI1; the interaction is phosphorylation-dependent occurs in a growth-dependent manner (By similarity). Interacts with KIT and CSF1R.By similarity1 Publication

Protein-protein interaction databases

BioGridi208177. 4 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ9JM90.
SMRiQ9JM90. Positions 16-152, 173-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 12197PHPROSITE-ProRule annotationAdd
BLAST
Domaini179 – 27395SH2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG47666.
GeneTreeiENSGT00530000063841.
HOGENOMiHOG000234375.
HOVERGENiHBG062262.
InParanoidiQ9JM90.
OMAiALPLYFE.
OrthoDBiEOG79KPFP.
PhylomeDBiQ9JM90.
TreeFamiTF332087.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9JM90-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLVKRSDHQ EYKHYWTELR
60 70 80 90 100
GTTLFFYTDK KSTIYVGKLD IIDLVCLTGQ HSTEKNCAKF TLVLPKEEVH
110 120 130 140 150
VKTENTESGE EWRGFILTVT ELTVPQHVSL LPGQVIRLHE VLEREKKRRI
160 170 180 190 200
ETDQLPLMPP EKEKEPVQDY ADVLNPLPEC FYAVSRKEAT AMLEKNPSWG
210 220 230 240 250
NMILRPGSDS KNYSITIRQE IEMPRIKHFK VTRTGNNYTI ELEKPVTLPN
260 270 280 290
LFSVIDYFVK ETRGNLRPFI HSADDNFGQD PNIEDRSEKF KKNPHNA
Length:297
Mass (Da):34,628
Last modified:October 1, 2000 - v1
Checksum:i8033C606990AAA75
GO
Isoform 2 (identifier: Q9JM90-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-127: LTVPQH → VIRFPL
     128-297: Missing.

Note: No experimental confirmation available.

Show »
Length:127
Mass (Da):14,921
Checksum:iAFAAE1584FA79F68
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei122 – 1276LTVPQH → VIRFPL in isoform 2. 1 PublicationVSP_013399
Alternative sequencei128 – 297170Missing in isoform 2. 1 PublicationVSP_013400Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036058 mRNA. Translation: BAA92531.1.
AK041474 mRNA. Translation: BAC30953.1.
AK162655 mRNA. Translation: BAE37007.1.
BC057898 mRNA. Translation: AAH57898.1.
BC145828 mRNA. Translation: AAI45829.1.
BC145830 mRNA. Translation: AAI45831.1.
CCDSiCCDS19377.1. [Q9JM90-1]
RefSeqiNP_064376.1. NM_019992.3. [Q9JM90-1]
XP_006535224.1. XM_006535161.1. [Q9JM90-2]
UniGeneiMm.131237.

Genome annotation databases

EnsembliENSMUST00000031171; ENSMUSP00000031171; ENSMUSG00000029254. [Q9JM90-1]
GeneIDi56792.
KEGGimmu:56792.
UCSCiuc008xxf.1. mouse. [Q9JM90-2]
uc008xxg.1. mouse. [Q9JM90-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036058 mRNA. Translation: BAA92531.1.
AK041474 mRNA. Translation: BAC30953.1.
AK162655 mRNA. Translation: BAE37007.1.
BC057898 mRNA. Translation: AAH57898.1.
BC145828 mRNA. Translation: AAI45829.1.
BC145830 mRNA. Translation: AAI45831.1.
CCDSiCCDS19377.1. [Q9JM90-1]
RefSeqiNP_064376.1. NM_019992.3. [Q9JM90-1]
XP_006535224.1. XM_006535161.1. [Q9JM90-2]
UniGeneiMm.131237.

3D structure databases

ProteinModelPortaliQ9JM90.
SMRiQ9JM90. Positions 16-152, 173-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208177. 4 interactions.

PTM databases

PhosphoSiteiQ9JM90.

Proteomic databases

MaxQBiQ9JM90.
PaxDbiQ9JM90.
PRIDEiQ9JM90.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031171; ENSMUSP00000031171; ENSMUSG00000029254. [Q9JM90-1]
GeneIDi56792.
KEGGimmu:56792.
UCSCiuc008xxf.1. mouse. [Q9JM90-2]
uc008xxg.1. mouse. [Q9JM90-1]

Organism-specific databases

CTDi26228.
MGIiMGI:1926193. Stap1.

Phylogenomic databases

eggNOGiNOG47666.
GeneTreeiENSGT00530000063841.
HOGENOMiHOG000234375.
HOVERGENiHBG062262.
InParanoidiQ9JM90.
OMAiALPLYFE.
OrthoDBiEOG79KPFP.
PhylomeDBiQ9JM90.
TreeFamiTF332087.

Miscellaneous databases

NextBioi313336.
PROiQ9JM90.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JM90.
CleanExiMM_STAP1.
GenevestigatoriQ9JM90.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR001849. PH_domain.
IPR011993. PH_like_dom.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of murine STAP-1, the stem-cell-specific adaptor protein containing PH and SH2 domains."
    Masuhara M., Nagao K., Nishikawa M., Sasaki M., Yoshimura A., Osawa M.
    Biochem. Biophys. Res. Commun. 268:697-703(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, INTERACTION WITH KIT AND CSF1R, TISSUE SPECIFICITY.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Bone and Thymus.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Brain and Mammary gland.
  4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.

Entry informationi

Entry nameiSTAP1_MOUSE
AccessioniPrimary (citable) accession number: Q9JM90
Secondary accession number(s): A6H6C6, Q3TRM1, Q6PES6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: October 1, 2000
Last modified: February 4, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.