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Protein

Serum response factor

Gene

Srf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (By similarity). Required for cardiac differentiation and maturation.By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi129 – 21890By similarityAdd
BLAST

GO - Molecular functioni

  1. chromatin binding Source: MGI
  2. chromatin DNA binding Source: MGI
  3. DNA binding Source: MGI
  4. protein homodimerization activity Source: MGI
  5. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
  6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
  7. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity Source: Ensembl
  8. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  9. RNA polymerase II transcription factor binding transcription factor activity Source: BHF-UCL
  10. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
  11. sequence-specific DNA binding transcription factor activity Source: MGI
  12. serum response element binding Source: MGI
  13. transcription factor binding Source: MGI

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. actin filament organization Source: MGI
  3. associative learning Source: MGI
  4. bronchus cartilage development Source: MGI
  5. cardiac myofibril assembly Source: MGI
  6. cardiac vascular smooth muscle cell differentiation Source: MGI
  7. cell-matrix adhesion Source: MGI
  8. cell migration involved in sprouting angiogenesis Source: MGI
  9. cellular response to glucose stimulus Source: Ensembl
  10. cellular senescence Source: MGI
  11. contractile actin filament bundle assembly Source: MGI
  12. developmental growth Source: MGI
  13. dorsal aorta morphogenesis Source: MGI
  14. epithelial cell-cell adhesion Source: MGI
  15. epithelial structure maintenance Source: MGI
  16. erythrocyte development Source: MGI
  17. eyelid development in camera-type eye Source: MGI
  18. gastrulation Source: MGI
  19. heart development Source: MGI
  20. heart looping Source: MGI
  21. heart trabecula formation Source: MGI
  22. hematopoietic stem cell differentiation Source: MGI
  23. hippocampus development Source: MGI
  24. in utero embryonic development Source: MGI
  25. leukocyte differentiation Source: MGI
  26. long-term memory Source: Ensembl
  27. long term synaptic depression Source: MGI
  28. lung morphogenesis Source: MGI
  29. lung smooth muscle development Source: MGI
  30. megakaryocyte development Source: MGI
  31. mesoderm formation Source: MGI
  32. morphogenesis of an epithelial sheet Source: MGI
  33. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
  34. muscle cell cellular homeostasis Source: MGI
  35. negative regulation of beta-amyloid clearance Source: MGI
  36. negative regulation of cell migration Source: Ensembl
  37. negative regulation of cell proliferation Source: Ensembl
  38. neuron migration Source: MGI
  39. neuron projection development Source: MGI
  40. patterning of blood vessels Source: MGI
  41. platelet activation Source: MGI
  42. platelet formation Source: MGI
  43. positive regulation of axon extension Source: MGI
  44. positive regulation of cell differentiation Source: MGI
  45. positive regulation of filopodium assembly Source: MGI
  46. positive regulation of sequence-specific DNA binding transcription factor activity Source: MGI
  47. positive regulation of smooth muscle contraction Source: MGI
  48. positive regulation of transcription, DNA-templated Source: UniProtKB
  49. positive regulation of transcription by glucose Source: Ensembl
  50. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  51. positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: BHF-UCL
  52. positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  53. positive regulation of transcription via serum response element binding Source: MGI
  54. positive thymic T cell selection Source: MGI
  55. primitive streak formation Source: MGI
  56. regulation of cell adhesion Source: MGI
  57. regulation of transcription, DNA-templated Source: MGI
  58. regulation of water loss via skin Source: MGI
  59. response to cytokine Source: MGI
  60. response to hormone Source: MGI
  61. response to hypoxia Source: Ensembl
  62. sarcomere organization Source: MGI
  63. single organismal cell-cell adhesion Source: MGI
  64. skin morphogenesis Source: MGI
  65. stress fiber assembly Source: MGI
  66. tangential migration from the subventricular zone to the olfactory bulb Source: MGI
  67. tight junction assembly Source: MGI
  68. trachea cartilage development Source: MGI
  69. transcription from RNA polymerase II promoter Source: MGI
  70. trophectodermal cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serum response factor
Short name:
SRF
Gene namesi
Name:Srf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:106658. Srf.

Subcellular locationi

  1. Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nuclear chromatin Source: BHF-UCL
  3. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice lacking Srf in cardiac tissue display lethal cardiac defects between E10.5 and E13.5 characterized by abnormally thin myocardium, dilated cardiac chambers, poor trabeculation and a disorganised interventricular septum.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Serum response factorPRO_0000245225Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei73 – 731PhosphoserineBy similarity
Modified residuei75 – 751PhosphoserineBy similarity
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei81 – 811PhosphoserineBy similarity
Modified residuei99 – 991PhosphoserineBy similarity
Modified residuei220 – 2201Phosphoserine2 Publications
Modified residuei249 – 2491PhosphoserineBy similarity
Glycosylationi273 – 2731O-linked (GlcNAc)By similarity
Glycosylationi303 – 3031O-linked (GlcNAc)By similarity
Glycosylationi305 – 3051O-linked (GlcNAc)By similarity
Glycosylationi312 – 3121O-linked (GlcNAc)By similarity
Glycosylationi379 – 3791O-linked (GlcNAc)By similarity
Modified residuei431 – 4311Phosphoserine; by dsDNA kinaseBy similarity
Modified residuei442 – 4421Phosphoserine; by dsDNA kinaseBy similarity

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9JM73.

PTM databases

PhosphoSiteiQ9JM73.

Expressioni

Gene expression databases

BgeeiQ9JM73.
CleanExiMM_SRF.
GenevestigatoriQ9JM73.

Interactioni

Subunit structurei

Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1 (By similarity). Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI. Interacts with LPXN (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Srebf2Q3U1N23EBI-493266,EBI-645275

Protein-protein interaction databases

BioGridi203497. 6 interactions.
DIPiDIP-49624N.
IntActiQ9JM73. 3 interactions.
MINTiMINT-1739318.
STRINGi10090.ENSMUSP00000015749.

Structurei

3D structure databases

ProteinModelPortaliQ9JM73.
SMRiQ9JM73. Positions 131-219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini137 – 19761MADS-boxPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni164 – 21855Involved in dimerizationBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi13 – 138126Gly-richAdd
BLAST
Compositional biasi76 – 8611Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi238 – 25417Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Contains 1 MADS-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5068.
GeneTreeiENSGT00400000022158.
HOGENOMiHOG000012380.
HOVERGENiHBG014968.
InParanoidiQ9JM73.
KOiK04378.
OMAiNAFPQAP.
OrthoDBiEOG76DTVJ.
PhylomeDBiQ9JM73.
TreeFamiTF318482.

Family and domain databases

InterProiIPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JM73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSQAGAAA ALGRGSALGG NLNRTPTGRP GGGGGTRGAN GGRVPGNGAG
60 70 80 90 100
LGQSRLEREA AAAAAPTAGA LYSGSEGDSE SGEEEELGAE RRGLKRSLSE
110 120 130 140 150
MELGVVVGGP EAAAAAAGGY GPVSGAVSGA KPGKKTRGRV KIKMEFIDNK
160 170 180 190 200
LRRYTTFSKR KTGIMKKAYE LSTLTGTQVL LLVASETGHV YTFATRKLQP
210 220 230 240 250
MITSETGKAL IQTCLNSPDS PPRSDPTTDQ RMSATGFEEP DLTYQVSESD
260 270 280 290 300
SSGETKDTLK PAFTVTNLPG TTSTIQTAPS TSTTMQVSSG PSFPITNYLA
310 320 330 340 350
PVSASVSPSA VSSANGTVLK STGSGPVSSG GLMQLPTSFT LMPGGAVAQQ
360 370 380 390 400
VPVQAIHVHQ APQQASPSRD SSTDLTQTSS SGTVTLPATI MTSSVPTTVG
410 420 430 440 450
GHMMYPSPHA VMYAPTSGLA DGSLTVLNAF SQAPSTMQVS HSQVQEPGGV
460 470 480 490 500
PQVFLTAPSG TVQIPVSAVQ LHQMAVIGQQ AGSSSNLTEL QVVNLDATHS

TKSE
Length:504
Mass (Da):51,247
Last modified:October 1, 2000 - v1
Checksum:i26353F18A2B46F6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038376 Genomic DNA. Translation: BAA92314.1.
BC051950 mRNA. Translation: AAH51950.1.
CCDSiCCDS28831.1.
RefSeqiNP_065239.1. NM_020493.2.
UniGeneiMm.45044.

Genome annotation databases

EnsembliENSMUST00000015749; ENSMUSP00000015749; ENSMUSG00000015605.
GeneIDi20807.
KEGGimmu:20807.
UCSCiuc008ctg.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038376 Genomic DNA. Translation: BAA92314.1.
BC051950 mRNA. Translation: AAH51950.1.
CCDSiCCDS28831.1.
RefSeqiNP_065239.1. NM_020493.2.
UniGeneiMm.45044.

3D structure databases

ProteinModelPortaliQ9JM73.
SMRiQ9JM73. Positions 131-219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203497. 6 interactions.
DIPiDIP-49624N.
IntActiQ9JM73. 3 interactions.
MINTiMINT-1739318.
STRINGi10090.ENSMUSP00000015749.

PTM databases

PhosphoSiteiQ9JM73.

Proteomic databases

PRIDEiQ9JM73.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015749; ENSMUSP00000015749; ENSMUSG00000015605.
GeneIDi20807.
KEGGimmu:20807.
UCSCiuc008ctg.1. mouse.

Organism-specific databases

CTDi6722.
MGIiMGI:106658. Srf.

Phylogenomic databases

eggNOGiCOG5068.
GeneTreeiENSGT00400000022158.
HOGENOMiHOG000012380.
HOVERGENiHBG014968.
InParanoidiQ9JM73.
KOiK04378.
OMAiNAFPQAP.
OrthoDBiEOG76DTVJ.
PhylomeDBiQ9JM73.
TreeFamiTF318482.

Miscellaneous databases

NextBioi299523.
PROiQ9JM73.
SOURCEiSearch...

Gene expression databases

BgeeiQ9JM73.
CleanExiMM_SRF.
GenevestigatoriQ9JM73.

Family and domain databases

InterProiIPR002100. TF_MADSbox.
[Graphical view]
PfamiPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSiPR00404. MADSDOMAIN.
SMARTiSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMiSSF55455. SSF55455. 1 hit.
PROSITEiPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Serum response factor."
    Miwa T.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Identification of a novel serum response factor cofactor in cardiac gene regulation."
    Zhang X., Azhar G., Zhong Y., Wei J.Y.
    J. Biol. Chem. 279:55626-55632(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SRFBP1, SUBUNIT.
  4. "Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality."
    Parlakian A., Tuil D., Hamard G., Tavernier G., Hentzen D., Concordet J.-P., Paulin D., Li Z., Daegelen D.
    Mol. Cell. Biol. 24:5281-5289(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Zipzap/p200 is a novel zinc finger protein contributing to cardiac gene regulation."
    Zhang X., Azhar G., Zhong Y., Wei J.Y.
    Biochem. Biophys. Res. Commun. 346:794-801(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH ARID2, SUBUNIT.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  8. "SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin."
    Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R.
    Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MKL1 AND SCAI, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiSRF_MOUSE
AccessioniPrimary (citable) accession number: Q9JM73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: March 4, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.