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Protein

Serum response factor

Gene

Srf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) (By similarity). Required for cardiac differentiation and maturation.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi129 – 218By similarityAdd BLAST90

GO - Molecular functioni

  • chromatin binding Source: MGI
  • chromatin DNA binding Source: MGI
  • DNA binding Source: MGI
  • DNA binding transcription factor activity Source: MGI
  • primary miRNA binding Source: BHF-UCL
  • protein homodimerization activity Source: MGI
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • sequence-specific DNA binding Source: MGI
  • serum response element binding Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: BHF-UCL
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: BHF-UCL
  • transcription factor activity, RNA polymerase II core promoter sequence-specific DNA binding Source: Ensembl
  • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding Source: MGI
  • transcription factor activity, RNA polymerase II transcription factor binding Source: BHF-UCL
  • transcription factor binding Source: MGI

GO - Biological processi

  • actin cytoskeleton organization Source: MGI
  • actin filament organization Source: MGI
  • associative learning Source: MGI
  • bicellular tight junction assembly Source: MGI
  • branching involved in blood vessel morphogenesis Source: MGI
  • bronchus cartilage development Source: MGI
  • cardiac myofibril assembly Source: MGI
  • cardiac vascular smooth muscle cell differentiation Source: MGI
  • cell-cell adhesion Source: MGI
  • cell-matrix adhesion Source: MGI
  • cell migration involved in sprouting angiogenesis Source: MGI
  • cellular response to glucose stimulus Source: Ensembl
  • cellular senescence Source: MGI
  • contractile actin filament bundle assembly Source: MGI
  • developmental growth Source: MGI
  • dorsal aorta morphogenesis Source: MGI
  • epithelial cell-cell adhesion Source: MGI
  • epithelial structure maintenance Source: MGI
  • erythrocyte development Source: MGI
  • eyelid development in camera-type eye Source: MGI
  • face development Source: MGI
  • forebrain development Source: MGI
  • gastrulation Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • heart trabecula formation Source: MGI
  • hematopoietic stem cell differentiation Source: MGI
  • hippocampus development Source: MGI
  • in utero embryonic development Source: MGI
  • leukocyte differentiation Source: MGI
  • long-term memory Source: Ensembl
  • long term synaptic depression Source: MGI
  • lung morphogenesis Source: MGI
  • lung smooth muscle development Source: MGI
  • megakaryocyte development Source: MGI
  • mesoderm formation Source: MGI
  • morphogenesis of an epithelial sheet Source: MGI
  • mRNA transcription by RNA polymerase II Source: BHF-UCL
  • muscle cell cellular homeostasis Source: MGI
  • negative regulation of amyloid-beta clearance Source: MGI
  • negative regulation of cell migration Source: Ensembl
  • negative regulation of cell proliferation Source: Ensembl
  • negative regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
  • neuron migration Source: MGI
  • neuron projection development Source: MGI
  • platelet activation Source: MGI
  • platelet formation Source: MGI
  • positive regulation of axon extension Source: MGI
  • positive regulation of cell differentiation Source: MGI
  • positive regulation of DNA binding transcription factor activity Source: MGI
  • positive regulation of filopodium assembly Source: MGI
  • positive regulation of pri-miRNA transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of smooth muscle contraction Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by glucose Source: Ensembl
  • positive regulation of transcription by RNA polymerase II Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: BHF-UCL
  • positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  • positive regulation of transcription via serum response element binding Source: MGI
  • positive thymic T cell selection Source: MGI
  • primitive streak formation Source: MGI
  • regulation of cell adhesion Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • regulation of water loss via skin Source: MGI
  • response to cytokine Source: MGI
  • response to hormone Source: MGI
  • response to hypoxia Source: Ensembl
  • sarcomere organization Source: MGI
  • skin morphogenesis Source: MGI
  • stress fiber assembly Source: MGI
  • tangential migration from the subventricular zone to the olfactory bulb Source: MGI
  • thymus development Source: MGI
  • thyroid gland development Source: MGI
  • trachea cartilage development Source: MGI
  • transcription by RNA polymerase II Source: MGI
  • trophectodermal cell differentiation Source: MGI

Keywordsi

Molecular functionActivator, Developmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins

Names & Taxonomyi

Protein namesi
Recommended name:
Serum response factor
Short name:
SRF
Gene namesi
Name:Srf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:106658 Srf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice lacking Srf in cardiac tissue display lethal cardiac defects between E10.5 and E13.5 characterized by abnormally thin myocardium, dilated cardiac chambers, poor trabeculation and a disorganised interventricular septum.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002452251 – 504Serum response factorAdd BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei73PhosphoserineBy similarity1
Modified residuei75PhosphoserineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei81PhosphoserineBy similarity1
Modified residuei99PhosphoserineBy similarity1
Modified residuei220PhosphoserineCombined sources1
Modified residuei249PhosphoserineBy similarity1
Glycosylationi273O-linked (GlcNAc) serineBy similarity1
Glycosylationi303O-linked (GlcNAc) serineBy similarity1
Glycosylationi305O-linked (GlcNAc) serineBy similarity1
Glycosylationi312O-linked (GlcNAc) serineBy similarity1
Glycosylationi379O-linked (GlcNAc) serineBy similarity1
Modified residuei431Phosphoserine; by dsDNA kinaseBy similarity1
Modified residuei442Phosphoserine; by dsDNA kinaseBy similarity1

Post-translational modificationi

Phosphorylated by PRKDC.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9JM73
PaxDbiQ9JM73
PeptideAtlasiQ9JM73
PRIDEiQ9JM73

PTM databases

iPTMnetiQ9JM73
PhosphoSitePlusiQ9JM73

Expressioni

Gene expression databases

BgeeiENSMUSG00000015605
CleanExiMM_SRF
GenevisibleiQ9JM73 MM

Interactioni

Subunit structurei

Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1 (By similarity). Interacts with ARID2 (PubMed:16782067). Interacts with SRFBP1 (PubMed:15492011). Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI (PubMed:19350017). Interacts with LPXN (By similarity). Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation (By similarity).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Srebf2Q3U1N23EBI-493266,EBI-645275

GO - Molecular functioni

Protein-protein interaction databases

BioGridi203497, 6 interactors
CORUMiQ9JM73
DIPiDIP-49624N
IntActiQ9JM73, 4 interactors
STRINGi10090.ENSMUSP00000015749

Structurei

3D structure databases

ProteinModelPortaliQ9JM73
SMRiQ9JM73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini137 – 197MADS-boxPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni164 – 218Involved in dimerizationBy similarityAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 138Gly-richAdd BLAST126
Compositional biasi76 – 86Asp/Glu-rich (acidic)Add BLAST11
Compositional biasi238 – 254Asp/Glu-rich (acidic)Add BLAST17

Phylogenomic databases

eggNOGiKOG0015 Eukaryota
COG5068 LUCA
GeneTreeiENSGT00400000022158
HOGENOMiHOG000012380
HOVERGENiHBG014968
InParanoidiQ9JM73
KOiK04378
OMAiTSTSMQV
OrthoDBiEOG091G0X24
PhylomeDBiQ9JM73
TreeFamiTF318482

Family and domain databases

CDDicd00266 MADS_SRF_like, 1 hit
Gene3Di3.40.1810.10, 1 hit
InterProiView protein in InterPro
IPR033897 MADS_SRF-like
IPR002100 TF_MADSbox
IPR036879 TF_MADSbox_sf
PfamiView protein in Pfam
PF00319 SRF-TF, 1 hit
PRINTSiPR00404 MADSDOMAIN
SMARTiView protein in SMART
SM00432 MADS, 1 hit
SUPFAMiSSF55455 SSF55455, 1 hit
PROSITEiView protein in PROSITE
PS00350 MADS_BOX_1, 1 hit
PS50066 MADS_BOX_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9JM73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPSQAGAAA ALGRGSALGG NLNRTPTGRP GGGGGTRGAN GGRVPGNGAG
60 70 80 90 100
LGQSRLEREA AAAAAPTAGA LYSGSEGDSE SGEEEELGAE RRGLKRSLSE
110 120 130 140 150
MELGVVVGGP EAAAAAAGGY GPVSGAVSGA KPGKKTRGRV KIKMEFIDNK
160 170 180 190 200
LRRYTTFSKR KTGIMKKAYE LSTLTGTQVL LLVASETGHV YTFATRKLQP
210 220 230 240 250
MITSETGKAL IQTCLNSPDS PPRSDPTTDQ RMSATGFEEP DLTYQVSESD
260 270 280 290 300
SSGETKDTLK PAFTVTNLPG TTSTIQTAPS TSTTMQVSSG PSFPITNYLA
310 320 330 340 350
PVSASVSPSA VSSANGTVLK STGSGPVSSG GLMQLPTSFT LMPGGAVAQQ
360 370 380 390 400
VPVQAIHVHQ APQQASPSRD SSTDLTQTSS SGTVTLPATI MTSSVPTTVG
410 420 430 440 450
GHMMYPSPHA VMYAPTSGLA DGSLTVLNAF SQAPSTMQVS HSQVQEPGGV
460 470 480 490 500
PQVFLTAPSG TVQIPVSAVQ LHQMAVIGQQ AGSSSNLTEL QVVNLDATHS

TKSE
Length:504
Mass (Da):51,247
Last modified:October 1, 2000 - v1
Checksum:i26353F18A2B46F6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB038376 Genomic DNA Translation: BAA92314.1
BC051950 mRNA Translation: AAH51950.1
CCDSiCCDS28831.1
RefSeqiNP_065239.1, NM_020493.2
UniGeneiMm.45044

Genome annotation databases

EnsembliENSMUST00000015749; ENSMUSP00000015749; ENSMUSG00000015605
GeneIDi20807
KEGGimmu:20807
UCSCiuc008ctg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSRF_MOUSE
AccessioniPrimary (citable) accession number: Q9JM73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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