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Q9JM73 (SRF_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serum response factor

Short name=SRF
Gene names
Name:Srf
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) By similarity. Required for cardiac differentiation and maturation.

Subunit structure

Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1 By similarity. Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI. Interacts with LPXN By similarity. Ref.3 Ref.5 Ref.8

Subcellular location

Nucleus Ref.8.

Post-translational modification

Phosphorylated by PRKDC By similarity.

Disruption phenotype

Mice lacking Srf in cardiac tissue display lethal cardiac defects between E10.5 and E13.5 characterized by abnormally thin myocardium, dilated cardiac chambers, poor trabeculation and a disorganised interventricular septum. Ref.4

Sequence similarities

Contains 1 MADS-box domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from mutant phenotype PubMed 15837932PubMed 20047077. Source: MGI

actin filament organization

Inferred from mutant phenotype PubMed 18804439. Source: MGI

associative learning

Inferred from mutant phenotype PubMed 16600861. Source: MGI

cardiac myofibril assembly

Inferred from mutant phenotype PubMed 15569937. Source: MGI

cardiac vascular smooth muscle cell differentiation

Inferred from mutant phenotype PubMed 15569937. Source: MGI

cell migration involved in sprouting angiogenesis

Inferred from mutant phenotype PubMed 18804439. Source: MGI

cell-matrix adhesion

Inferred from mutant phenotype PubMed 20525922PubMed 20709909PubMed 21401944. Source: MGI

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular senescence

Inferred from electronic annotation. Source: Ensembl

contractile actin filament bundle assembly

Inferred from mutant phenotype PubMed 15569937. Source: MGI

developmental growth

Inferred from mutant phenotype PubMed 9799237. Source: MGI

dorsal aorta morphogenesis

Inferred from mutant phenotype PubMed 15569937. Source: MGI

epithelial cell-cell adhesion

Inferred from mutant phenotype PubMed 20047077. Source: MGI

epithelial structure maintenance

Inferred from mutant phenotype PubMed 20047077. Source: MGI

erythrocyte development

Inferred from mutant phenotype PubMed 20709909. Source: MGI

eyelid development in camera-type eye

Inferred from mutant phenotype PubMed 20047077. Source: MGI

gastrulation

Inferred from mutant phenotype PubMed 9799237. Source: MGI

heart development

Inferred from mutant phenotype Ref.4PubMed 15929941. Source: MGI

heart looping

Inferred from mutant phenotype PubMed 15929941. Source: MGI

heart trabecula formation

Inferred from mutant phenotype PubMed 15569937. Source: MGI

hematopoietic stem cell differentiation

Inferred from mutant phenotype PubMed 20709909. Source: MGI

hippocampus development

Inferred from mutant phenotype PubMed 15837932. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 9799237. Source: MGI

leukocyte differentiation

Inferred from mutant phenotype PubMed 20709909. Source: MGI

long term synaptic depression

Inferred from mutant phenotype PubMed 16600861. Source: MGI

long-term memory

Inferred from electronic annotation. Source: Ensembl

mRNA transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19783823. Source: BHF-UCL

megakaryocyte development

Inferred from mutant phenotype PubMed 20525922PubMed 20709909. Source: MGI

mesoderm formation

Inferred from mutant phenotype PubMed 11060034PubMed 9799237. Source: MGI

morphogenesis of an epithelial sheet

Inferred from mutant phenotype PubMed 20047077. Source: MGI

muscle cell cellular homeostasis

Inferred from mutant phenotype PubMed 15647354. Source: MGI

negative regulation of beta-amyloid clearance

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell migration

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

neuron migration

Inferred from mutant phenotype PubMed 15837932. Source: MGI

neuron projection development

Inferred from mutant phenotype PubMed 19357276. Source: MGI

patterning of blood vessels

Inferred from mutant phenotype PubMed 18804439. Source: MGI

platelet activation

Inferred from mutant phenotype PubMed 20525922. Source: MGI

platelet formation

Inferred from mutant phenotype PubMed 20525922PubMed 20709909. Source: MGI

positive regulation of cell differentiation

Inferred from electronic annotation. Source: Ensembl

positive regulation of filopodium assembly

Inferred from mutant phenotype PubMed 18804439. Source: MGI

positive regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

positive regulation of smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription by glucose

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 11278942PubMed 8900044. Source: MGI

positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation

Inferred from mutant phenotype PubMed 19783823. Source: BHF-UCL

positive regulation of transcription initiation from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription via serum response element binding

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 19797053. Source: UniProtKB

positive thymic T cell selection

Inferred from mutant phenotype PubMed 21098124. Source: MGI

primitive streak formation

Inferred from mutant phenotype PubMed 9799237. Source: MGI

regulation of cell adhesion

Inferred from mutant phenotype PubMed 18804439. Source: MGI

regulation of transcription, DNA-templated

Inferred from direct assay PubMed 12525493. Source: MGI

regulation of water loss via skin

Inferred from mutant phenotype PubMed 20047077. Source: MGI

response to cytokine

Inferred from electronic annotation. Source: Ensembl

response to hormone

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

sarcomere organization

Inferred from mutant phenotype PubMed 15569937. Source: MGI

single organismal cell-cell adhesion

Inferred from mutant phenotype PubMed 21401944. Source: MGI

skin morphogenesis

Inferred from mutant phenotype PubMed 20047077. Source: MGI

stress fiber assembly

Inferred from mutant phenotype PubMed 20525922. Source: MGI

tangential migration from the subventricular zone to the olfactory bulb

Inferred from mutant phenotype PubMed 15837932. Source: MGI

tight junction assembly

Inferred from mutant phenotype PubMed 20047077. Source: MGI

trophectodermal cell differentiation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17576768. Source: MGI

nuclear chromatin

Inferred from direct assay PubMed 16427017. Source: BHF-UCL

nucleus

Inferred from direct assay PubMed 15929941PubMed 17576768. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12486129PubMed 17030628PubMed 21098124PubMed 8900044. Source: MGI

RNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 19783823. Source: BHF-UCL

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 19783823. Source: BHF-UCL

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 21379568. Source: MGI

RNA polymerase II transcription factor binding transcription factor activity

Inferred from direct assay PubMed 11439182. Source: BHF-UCL

RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 19783823. Source: BHF-UCL

chromatin DNA binding

Inferred from direct assay PubMed 18458156. Source: MGI

chromatin binding

Inferred from direct assay PubMed 21379568. Source: MGI

protein binding

Inferred from physical interaction PubMed 12397177Ref.8. Source: UniProtKB

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 12525493PubMed 8900044. Source: MGI

serum response element binding

Inferred from direct assay PubMed 2062642. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Srebf2Q3U1N23EBI-493266,EBI-645275

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Serum response factor
PRO_0000245225

Regions

Domain137 – 19761MADS-box
DNA binding129 – 21890 By similarity
Region164 – 21855Involved in dimerization By similarity
Compositional bias13 – 138126Gly-rich
Compositional bias76 – 8611Asp/Glu-rich (acidic)
Compositional bias238 – 25417Asp/Glu-rich (acidic)

Amino acid modifications

Modified residue731Phosphoserine By similarity
Modified residue751Phosphoserine By similarity
Modified residue791Phosphoserine By similarity
Modified residue811Phosphoserine By similarity
Modified residue991Phosphoserine By similarity
Modified residue2201Phosphoserine Ref.6 Ref.7
Modified residue2491Phosphoserine By similarity
Modified residue4311Phosphoserine; by dsDNA kinase By similarity
Modified residue4421Phosphoserine; by dsDNA kinase By similarity
Glycosylation2731O-linked (GlcNAc) By similarity
Glycosylation3031O-linked (GlcNAc) By similarity
Glycosylation3051O-linked (GlcNAc) By similarity
Glycosylation3121O-linked (GlcNAc) By similarity
Glycosylation3791O-linked (GlcNAc) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9JM73 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 26353F18A2B46F6B

FASTA50451,247
        10         20         30         40         50         60 
MLPSQAGAAA ALGRGSALGG NLNRTPTGRP GGGGGTRGAN GGRVPGNGAG LGQSRLEREA 

        70         80         90        100        110        120 
AAAAAPTAGA LYSGSEGDSE SGEEEELGAE RRGLKRSLSE MELGVVVGGP EAAAAAAGGY 

       130        140        150        160        170        180 
GPVSGAVSGA KPGKKTRGRV KIKMEFIDNK LRRYTTFSKR KTGIMKKAYE LSTLTGTQVL 

       190        200        210        220        230        240 
LLVASETGHV YTFATRKLQP MITSETGKAL IQTCLNSPDS PPRSDPTTDQ RMSATGFEEP 

       250        260        270        280        290        300 
DLTYQVSESD SSGETKDTLK PAFTVTNLPG TTSTIQTAPS TSTTMQVSSG PSFPITNYLA 

       310        320        330        340        350        360 
PVSASVSPSA VSSANGTVLK STGSGPVSSG GLMQLPTSFT LMPGGAVAQQ VPVQAIHVHQ 

       370        380        390        400        410        420 
APQQASPSRD SSTDLTQTSS SGTVTLPATI MTSSVPTTVG GHMMYPSPHA VMYAPTSGLA 

       430        440        450        460        470        480 
DGSLTVLNAF SQAPSTMQVS HSQVQEPGGV PQVFLTAPSG TVQIPVSAVQ LHQMAVIGQQ 

       490        500 
AGSSSNLTEL QVVNLDATHS TKSE 

« Hide

References

« Hide 'large scale' references
[1]"Serum response factor."
Miwa T.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"Identification of a novel serum response factor cofactor in cardiac gene regulation."
Zhang X., Azhar G., Zhong Y., Wei J.Y.
J. Biol. Chem. 279:55626-55632(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SRFBP1, SUBUNIT.
[4]"Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality."
Parlakian A., Tuil D., Hamard G., Tavernier G., Hentzen D., Concordet J.-P., Paulin D., Li Z., Daegelen D.
Mol. Cell. Biol. 24:5281-5289(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"Zipzap/p200 is a novel zinc finger protein contributing to cardiac gene regulation."
Zhang X., Azhar G., Zhong Y., Wei J.Y.
Biochem. Biophys. Res. Commun. 346:794-801(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARID2, SUBUNIT.
[6]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[7]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[8]"SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin."
Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R.
Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH MKL1 AND SCAI, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB038376 Genomic DNA. Translation: BAA92314.1.
BC051950 mRNA. Translation: AAH51950.1.
CCDSCCDS28831.1.
RefSeqNP_065239.1. NM_020493.2.
UniGeneMm.45044.

3D structure databases

ProteinModelPortalQ9JM73.
SMRQ9JM73. Positions 131-219.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203497. 6 interactions.
IntActQ9JM73. 3 interactions.
MINTMINT-1739318.
STRING10090.ENSMUSP00000015749.

PTM databases

PhosphoSiteQ9JM73.

Proteomic databases

PRIDEQ9JM73.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000015749; ENSMUSP00000015749; ENSMUSG00000015605.
GeneID20807.
KEGGmmu:20807.
UCSCuc008ctg.1. mouse.

Organism-specific databases

CTD6722.
MGIMGI:106658. Srf.

Phylogenomic databases

eggNOGCOG5068.
GeneTreeENSGT00400000022158.
HOGENOMHOG000012380.
HOVERGENHBG014968.
InParanoidQ9JM73.
KOK04378.
OMANAFPQAP.
OrthoDBEOG76DTVJ.
PhylomeDBQ9JM73.
TreeFamTF318482.

Gene expression databases

BgeeQ9JM73.
CleanExMM_SRF.
GenevestigatorQ9JM73.

Family and domain databases

InterProIPR002100. TF_MADSbox.
[Graphical view]
PfamPF00319. SRF-TF. 1 hit.
[Graphical view]
PRINTSPR00404. MADSDOMAIN.
SMARTSM00432. MADS. 1 hit.
[Graphical view]
SUPFAMSSF55455. SSF55455. 1 hit.
PROSITEPS00350. MADS_BOX_1. 1 hit.
PS50066. MADS_BOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio299523.
PROQ9JM73.
SOURCESearch...

Entry information

Entry nameSRF_MOUSE
AccessionPrimary (citable) accession number: Q9JM73
Entry history
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: October 1, 2000
Last modified: July 9, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot