Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9JM73

- SRF_MOUSE

UniProt

Q9JM73 - SRF_MOUSE

Protein

Serum response factor

Gene

Srf

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS) By similarity. Required for cardiac differentiation and maturation.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi129 – 21890By similarityAdd
    BLAST

    GO - Molecular functioni

    1. chromatin binding Source: MGI
    2. chromatin DNA binding Source: MGI
    3. DNA binding Source: MGI
    4. protein binding Source: UniProtKB
    5. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: BHF-UCL
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
    7. RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity Source: Ensembl
    8. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    9. RNA polymerase II transcription factor binding transcription factor activity Source: BHF-UCL
    10. RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: BHF-UCL
    11. sequence-specific DNA binding transcription factor activity Source: MGI
    12. serum response element binding Source: MGI

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. actin filament organization Source: MGI
    3. associative learning Source: MGI
    4. cardiac myofibril assembly Source: MGI
    5. cardiac vascular smooth muscle cell differentiation Source: MGI
    6. cell-matrix adhesion Source: MGI
    7. cell migration involved in sprouting angiogenesis Source: MGI
    8. cellular response to glucose stimulus Source: Ensembl
    9. cellular senescence Source: Ensembl
    10. contractile actin filament bundle assembly Source: MGI
    11. developmental growth Source: MGI
    12. dorsal aorta morphogenesis Source: MGI
    13. epithelial cell-cell adhesion Source: MGI
    14. epithelial structure maintenance Source: MGI
    15. erythrocyte development Source: MGI
    16. eyelid development in camera-type eye Source: MGI
    17. gastrulation Source: MGI
    18. heart development Source: MGI
    19. heart looping Source: MGI
    20. heart trabecula formation Source: MGI
    21. hematopoietic stem cell differentiation Source: MGI
    22. hippocampus development Source: MGI
    23. in utero embryonic development Source: MGI
    24. leukocyte differentiation Source: MGI
    25. long-term memory Source: Ensembl
    26. long term synaptic depression Source: MGI
    27. megakaryocyte development Source: MGI
    28. mesoderm formation Source: MGI
    29. morphogenesis of an epithelial sheet Source: MGI
    30. mRNA transcription from RNA polymerase II promoter Source: BHF-UCL
    31. muscle cell cellular homeostasis Source: MGI
    32. negative regulation of beta-amyloid clearance Source: Ensembl
    33. negative regulation of cell migration Source: Ensembl
    34. negative regulation of cell proliferation Source: Ensembl
    35. neuron migration Source: MGI
    36. neuron projection development Source: MGI
    37. patterning of blood vessels Source: MGI
    38. platelet activation Source: MGI
    39. platelet formation Source: MGI
    40. positive regulation of cell differentiation Source: Ensembl
    41. positive regulation of filopodium assembly Source: MGI
    42. positive regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    43. positive regulation of smooth muscle contraction Source: Ensembl
    44. positive regulation of transcription, DNA-templated Source: UniProtKB
    45. positive regulation of transcription by glucose Source: Ensembl
    46. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    47. positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation Source: BHF-UCL
    48. positive regulation of transcription initiation from RNA polymerase II promoter Source: Ensembl
    49. positive regulation of transcription via serum response element binding Source: Ensembl
    50. positive thymic T cell selection Source: MGI
    51. primitive streak formation Source: MGI
    52. regulation of cell adhesion Source: MGI
    53. regulation of transcription, DNA-templated Source: MGI
    54. regulation of water loss via skin Source: MGI
    55. response to cytokine Source: Ensembl
    56. response to hormone Source: Ensembl
    57. response to hypoxia Source: Ensembl
    58. sarcomere organization Source: MGI
    59. single organismal cell-cell adhesion Source: MGI
    60. skin morphogenesis Source: MGI
    61. stress fiber assembly Source: MGI
    62. tangential migration from the subventricular zone to the olfactory bulb Source: MGI
    63. tight junction assembly Source: MGI
    64. trophectodermal cell differentiation Source: Ensembl

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serum response factor
    Short name:
    SRF
    Gene namesi
    Name:Srf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:106658. Srf.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nuclear chromatin Source: BHF-UCL
    3. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Mice lacking Srf in cardiac tissue display lethal cardiac defects between E10.5 and E13.5 characterized by abnormally thin myocardium, dilated cardiac chambers, poor trabeculation and a disorganised interventricular septum.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Serum response factorPRO_0000245225Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei73 – 731PhosphoserineBy similarity
    Modified residuei75 – 751PhosphoserineBy similarity
    Modified residuei79 – 791PhosphoserineBy similarity
    Modified residuei81 – 811PhosphoserineBy similarity
    Modified residuei99 – 991PhosphoserineBy similarity
    Modified residuei220 – 2201Phosphoserine2 Publications
    Modified residuei249 – 2491PhosphoserineBy similarity
    Glycosylationi273 – 2731O-linked (GlcNAc)By similarity
    Glycosylationi303 – 3031O-linked (GlcNAc)By similarity
    Glycosylationi305 – 3051O-linked (GlcNAc)By similarity
    Glycosylationi312 – 3121O-linked (GlcNAc)By similarity
    Glycosylationi379 – 3791O-linked (GlcNAc)By similarity
    Modified residuei431 – 4311Phosphoserine; by dsDNA kinaseBy similarity
    Modified residuei442 – 4421Phosphoserine; by dsDNA kinaseBy similarity

    Post-translational modificationi

    Phosphorylated by PRKDC.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiQ9JM73.

    PTM databases

    PhosphoSiteiQ9JM73.

    Expressioni

    Gene expression databases

    BgeeiQ9JM73.
    CleanExiMM_SRF.
    GenevestigatoriQ9JM73.

    Interactioni

    Subunit structurei

    Binds DNA as a multimer, probably a dimer. Interacts with MLLT7/FOXO4, NKX3A and SSRP1 By similarity. Interacts with ARID2 and SRFBP1. Forms complexes with ARID2, MYOCD, NKX2-5 and SRFBP1. Forms a nuclear ternary complex with MKL1 and SCAI. Interacts with LPXN By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Srebf2Q3U1N23EBI-493266,EBI-645275

    Protein-protein interaction databases

    BioGridi203497. 6 interactions.
    DIPiDIP-49624N.
    IntActiQ9JM73. 3 interactions.
    MINTiMINT-1739318.
    STRINGi10090.ENSMUSP00000015749.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9JM73.
    SMRiQ9JM73. Positions 131-219.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini137 – 19761MADS-boxPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni164 – 21855Involved in dimerizationBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi13 – 138126Gly-richAdd
    BLAST
    Compositional biasi76 – 8611Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi238 – 25417Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Contains 1 MADS-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5068.
    GeneTreeiENSGT00400000022158.
    HOGENOMiHOG000012380.
    HOVERGENiHBG014968.
    InParanoidiQ9JM73.
    KOiK04378.
    OMAiNAFPQAP.
    OrthoDBiEOG76DTVJ.
    PhylomeDBiQ9JM73.
    TreeFamiTF318482.

    Family and domain databases

    InterProiIPR002100. TF_MADSbox.
    [Graphical view]
    PfamiPF00319. SRF-TF. 1 hit.
    [Graphical view]
    PRINTSiPR00404. MADSDOMAIN.
    SMARTiSM00432. MADS. 1 hit.
    [Graphical view]
    SUPFAMiSSF55455. SSF55455. 1 hit.
    PROSITEiPS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9JM73-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPSQAGAAA ALGRGSALGG NLNRTPTGRP GGGGGTRGAN GGRVPGNGAG    50
    LGQSRLEREA AAAAAPTAGA LYSGSEGDSE SGEEEELGAE RRGLKRSLSE 100
    MELGVVVGGP EAAAAAAGGY GPVSGAVSGA KPGKKTRGRV KIKMEFIDNK 150
    LRRYTTFSKR KTGIMKKAYE LSTLTGTQVL LLVASETGHV YTFATRKLQP 200
    MITSETGKAL IQTCLNSPDS PPRSDPTTDQ RMSATGFEEP DLTYQVSESD 250
    SSGETKDTLK PAFTVTNLPG TTSTIQTAPS TSTTMQVSSG PSFPITNYLA 300
    PVSASVSPSA VSSANGTVLK STGSGPVSSG GLMQLPTSFT LMPGGAVAQQ 350
    VPVQAIHVHQ APQQASPSRD SSTDLTQTSS SGTVTLPATI MTSSVPTTVG 400
    GHMMYPSPHA VMYAPTSGLA DGSLTVLNAF SQAPSTMQVS HSQVQEPGGV 450
    PQVFLTAPSG TVQIPVSAVQ LHQMAVIGQQ AGSSSNLTEL QVVNLDATHS 500
    TKSE 504
    Length:504
    Mass (Da):51,247
    Last modified:October 1, 2000 - v1
    Checksum:i26353F18A2B46F6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038376 Genomic DNA. Translation: BAA92314.1.
    BC051950 mRNA. Translation: AAH51950.1.
    CCDSiCCDS28831.1.
    RefSeqiNP_065239.1. NM_020493.2.
    UniGeneiMm.45044.

    Genome annotation databases

    EnsembliENSMUST00000015749; ENSMUSP00000015749; ENSMUSG00000015605.
    GeneIDi20807.
    KEGGimmu:20807.
    UCSCiuc008ctg.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB038376 Genomic DNA. Translation: BAA92314.1 .
    BC051950 mRNA. Translation: AAH51950.1 .
    CCDSi CCDS28831.1.
    RefSeqi NP_065239.1. NM_020493.2.
    UniGenei Mm.45044.

    3D structure databases

    ProteinModelPortali Q9JM73.
    SMRi Q9JM73. Positions 131-219.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203497. 6 interactions.
    DIPi DIP-49624N.
    IntActi Q9JM73. 3 interactions.
    MINTi MINT-1739318.
    STRINGi 10090.ENSMUSP00000015749.

    PTM databases

    PhosphoSitei Q9JM73.

    Proteomic databases

    PRIDEi Q9JM73.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000015749 ; ENSMUSP00000015749 ; ENSMUSG00000015605 .
    GeneIDi 20807.
    KEGGi mmu:20807.
    UCSCi uc008ctg.1. mouse.

    Organism-specific databases

    CTDi 6722.
    MGIi MGI:106658. Srf.

    Phylogenomic databases

    eggNOGi COG5068.
    GeneTreei ENSGT00400000022158.
    HOGENOMi HOG000012380.
    HOVERGENi HBG014968.
    InParanoidi Q9JM73.
    KOi K04378.
    OMAi NAFPQAP.
    OrthoDBi EOG76DTVJ.
    PhylomeDBi Q9JM73.
    TreeFami TF318482.

    Miscellaneous databases

    NextBioi 299523.
    PROi Q9JM73.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9JM73.
    CleanExi MM_SRF.
    Genevestigatori Q9JM73.

    Family and domain databases

    InterProi IPR002100. TF_MADSbox.
    [Graphical view ]
    Pfami PF00319. SRF-TF. 1 hit.
    [Graphical view ]
    PRINTSi PR00404. MADSDOMAIN.
    SMARTi SM00432. MADS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55455. SSF55455. 1 hit.
    PROSITEi PS00350. MADS_BOX_1. 1 hit.
    PS50066. MADS_BOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Serum response factor."
      Miwa T.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Identification of a novel serum response factor cofactor in cardiac gene regulation."
      Zhang X., Azhar G., Zhong Y., Wei J.Y.
      J. Biol. Chem. 279:55626-55632(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SRFBP1, SUBUNIT.
    4. "Targeted inactivation of serum response factor in the developing heart results in myocardial defects and embryonic lethality."
      Parlakian A., Tuil D., Hamard G., Tavernier G., Hentzen D., Concordet J.-P., Paulin D., Li Z., Daegelen D.
      Mol. Cell. Biol. 24:5281-5289(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Zipzap/p200 is a novel zinc finger protein contributing to cardiac gene regulation."
      Zhang X., Azhar G., Zhong Y., Wei J.Y.
      Biochem. Biophys. Res. Commun. 346:794-801(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ARID2, SUBUNIT.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-220, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    8. "SCAI acts as a suppressor of cancer cell invasion through the transcriptional control of beta1-integrin."
      Brandt D.T., Baarlink C., Kitzing T.M., Kremmer E., Ivaska J., Nollau P., Grosse R.
      Nat. Cell Biol. 11:557-568(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MKL1 AND SCAI, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiSRF_MOUSE
    AccessioniPrimary (citable) accession number: Q9JM73
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2006
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3