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Protein

Kallikrein 1-related peptidase b27

Gene

Klk1b27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease with chymotrypsin-like cleavage specificity. Shows activity towards casein, gelatin, IGFBP3 and fibronectin but not towards laminin or collagens I and IV. Does not hydrolyze kininogin to release Lys-bradykinin.

Enzyme regulationi

Strongly inhibited by protease inhibitors diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride and SBTI.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei65 – 651Charge relay systemBy similarity
Active sitei122 – 1221Charge relay systemBy similarity
Active sitei215 – 2151Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiS01.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b27 (EC:3.4.21.-)
Alternative name(s):
Glandular kallikrein K27
Short name:
mGK-27
Tissue kallikrein 27
Short name:
mKlk27
Gene namesi
Name:Klk1b27
Synonyms:Klk-27, Klk27
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:891980. Klk1b27.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi209 – 2091G → D: Loss of chymotryptic specificity and acquisition of trypsin-like cleavage specificity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence analysisAdd
BLAST
Propeptidei18 – 247Activation peptideBy similarityPRO_0000027995
Chaini25 – 263239Kallikrein 1-related peptidase b27PRO_0000027996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi31 ↔ 175PROSITE-ProRule annotationBy similarity
Disulfide bondi50 ↔ 66PROSITE-ProRule annotationBy similarity
Glycosylationi69 – 691N-linked (GlcNAc...)Curated
Glycosylationi105 – 1051N-linked (GlcNAc...)Curated
Disulfide bondi154 ↔ 221PROSITE-ProRule annotationBy similarity
Disulfide bondi186 ↔ 200PROSITE-ProRule annotationBy similarity
Disulfide bondi211 ↔ 236PROSITE-ProRule annotationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9JM71.
PaxDbiQ9JM71.
PRIDEiQ9JM71.

Expressioni

Tissue specificityi

Expressed in testis and submaxillary gland. Not expressed in heart, brain, spleen, lung, liver, muscle, kidney and ovary. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

Developmental stagei

Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

Gene expression databases

BgeeiENSMUSG00000063177.
CleanExiMM_KLK1B27.
GenevisibleiQ9JM71. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078786.

Structurei

3D structure databases

ProteinModelPortaliQ9JM71.
SMRiQ9JM71. Positions 25-263.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 260236Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9JM71.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9JM71.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JM71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP VQSRIIGGFK CKKNSQPWHV AVLRSNKYIC
60 70 80 90 100
GGVLLDPNWV LTAAHCYGND TSQHNVWLGK NKLFQREPSA QHRWVSKSFP
110 120 130 140 150
HPDYNMSLLN DHIPHPEDKS NDLMLLRLSK PADITDAVKP IDLPTEEPKL
160 170 180 190 200
GSTCLASGWG SITPTKYQIP NDLQCVFIKL LPNENCAKAY VHKVTDVMLC
210 220 230 240 250
VGETGGGKGT CKGDSGGPLI CDGVLHGITS WGSIPCAKPN APGVFTKLIK
260
FTSWIKDTMA KNP
Length:263
Mass (Da):28,742
Last modified:October 1, 2000 - v1
Checksum:i2B396C2DD3B359FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039275 mRNA. Translation: BAA92318.1.
BC132090 mRNA. Translation: AAI32091.1.
BC132665 mRNA. Translation: AAI32666.1.
CCDSiCCDS21194.1.
RefSeqiNP_064664.1. NM_020268.3.
UniGeneiMm.445911.

Genome annotation databases

EnsembliENSMUST00000079859; ENSMUSP00000078786; ENSMUSG00000063177.
GeneIDi16619.
KEGGimmu:16619.
UCSCiuc009gog.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039275 mRNA. Translation: BAA92318.1.
BC132090 mRNA. Translation: AAI32091.1.
BC132665 mRNA. Translation: AAI32666.1.
CCDSiCCDS21194.1.
RefSeqiNP_064664.1. NM_020268.3.
UniGeneiMm.445911.

3D structure databases

ProteinModelPortaliQ9JM71.
SMRiQ9JM71. Positions 25-263.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078786.

Protein family/group databases

MEROPSiS01.073.

Proteomic databases

MaxQBiQ9JM71.
PaxDbiQ9JM71.
PRIDEiQ9JM71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079859; ENSMUSP00000078786; ENSMUSG00000063177.
GeneIDi16619.
KEGGimmu:16619.
UCSCiuc009gog.1. mouse.

Organism-specific databases

CTDi16619.
MGIiMGI:891980. Klk1b27.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiQ9JM71.
OrthoDBiEOG091G0DF7.
PhylomeDBiQ9JM71.
TreeFamiTF331065.

Enzyme and pathway databases

ReactomeiR-MMU-1592389. Activation of Matrix Metalloproteinases.
R-MMU-381426. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

PROiQ9JM71.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000063177.
CleanExiMM_KLK1B27.
GenevisibleiQ9JM71. MM.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiIPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK1B27_MOUSE
AccessioniPrimary (citable) accession number: Q9JM71
Secondary accession number(s): A2RSF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.