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Protein

Kallikrein 1-related peptidase b27

Gene

Klk1b27

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine protease with chymotrypsin-like cleavage specificity. Shows activity towards casein, gelatin, IGFBP3 and fibronectin but not towards laminin or collagens I and IV. Does not hydrolyze kininogin to release Lys-bradykinin.

Enzyme regulationi

Strongly inhibited by protease inhibitors diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride and SBTI.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei65Charge relay systemBy similarity1
Active sitei122Charge relay systemBy similarity1
Active sitei215Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

  • proteolysis Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.073

Names & Taxonomyi

Protein namesi
Recommended name:
Kallikrein 1-related peptidase b27 (EC:3.4.21.-)
Alternative name(s):
Glandular kallikrein K27
Short name:
mGK-27
Tissue kallikrein 27
Short name:
mKlk27
Gene namesi
Name:Klk1b27
Synonyms:Klk-27, Klk27
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:891980 Klk1b27

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi209G → D: Loss of chymotryptic specificity and acquisition of trypsin-like cleavage specificity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002799518 – 24Activation peptideBy similarity7
ChainiPRO_000002799625 – 263Kallikrein 1-related peptidase b27Add BLAST239

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi31 ↔ 175PROSITE-ProRule annotationBy similarity
Disulfide bondi50 ↔ 66PROSITE-ProRule annotationBy similarity
Glycosylationi69N-linked (GlcNAc...) asparagineCurated1
Glycosylationi105N-linked (GlcNAc...) asparagineCurated1
Disulfide bondi154 ↔ 221PROSITE-ProRule annotationBy similarity
Disulfide bondi186 ↔ 200PROSITE-ProRule annotationBy similarity
Disulfide bondi211 ↔ 236PROSITE-ProRule annotationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9JM71
PaxDbiQ9JM71
PRIDEiQ9JM71

Expressioni

Tissue specificityi

Expressed in testis and submaxillary gland. Not expressed in heart, brain, spleen, lung, liver, muscle, kidney and ovary. In the testis, expression localized specifically to Leydig cells in the interstitial tissues.1 Publication

Developmental stagei

Detectable in testis 4 weeks after birth, becoming more prominent thereafter.1 Publication

Gene expression databases

BgeeiENSMUSG00000063177
CleanExiMM_KLK1B27
GenevisibleiQ9JM71 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000078786

Structurei

3D structure databases

ProteinModelPortaliQ9JM71
SMRiQ9JM71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini25 – 260Peptidase S1PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Belongs to the peptidase S1 family. Kallikrein subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00910000144067
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9JM71
OrthoDBiEOG091G0DF7
PhylomeDBiQ9JM71
TreeFamiTF331065

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9JM71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFLILFLAL SLGGIDAAPP VQSRIIGGFK CKKNSQPWHV AVLRSNKYIC
60 70 80 90 100
GGVLLDPNWV LTAAHCYGND TSQHNVWLGK NKLFQREPSA QHRWVSKSFP
110 120 130 140 150
HPDYNMSLLN DHIPHPEDKS NDLMLLRLSK PADITDAVKP IDLPTEEPKL
160 170 180 190 200
GSTCLASGWG SITPTKYQIP NDLQCVFIKL LPNENCAKAY VHKVTDVMLC
210 220 230 240 250
VGETGGGKGT CKGDSGGPLI CDGVLHGITS WGSIPCAKPN APGVFTKLIK
260
FTSWIKDTMA KNP
Length:263
Mass (Da):28,742
Last modified:October 1, 2000 - v1
Checksum:i2B396C2DD3B359FB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039275 mRNA Translation: BAA92318.1
BC132090 mRNA Translation: AAI32091.1
BC132665 mRNA Translation: AAI32666.1
CCDSiCCDS21194.1
RefSeqiNP_064664.1, NM_020268.3
UniGeneiMm.445911

Genome annotation databases

EnsembliENSMUST00000079859; ENSMUSP00000078786; ENSMUSG00000063177
GeneIDi16619
KEGGimmu:16619
UCSCiuc009gog.1 mouse

Similar proteinsi

Entry informationi

Entry nameiK1B27_MOUSE
AccessioniPrimary (citable) accession number: Q9JM71
Secondary accession number(s): A2RSF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: October 1, 2000
Last modified: April 25, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health