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Protein

ATP-sensitive inward rectifier potassium channel 10

Gene

Kcnj10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium (By similarity). In the kidney, together with KCNJ16, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei158 – 1581Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi210 – 2178ATPSequence analysis

GO - Molecular functioni

  • ATP-activated inward rectifier potassium channel activity Source: GO_Central
  • ATP binding Source: UniProtKB-KW
  • inward rectifier potassium channel activity Source: MGI
  • potassium channel activity Source: MGI

GO - Biological processi

  • adult walking behavior Source: MGI
  • central nervous system myelination Source: MGI
  • glutamate reuptake Source: MGI
  • oligodendrocyte development Source: MGI
  • potassium ion homeostasis Source: MGI
  • potassium ion import Source: GO_Central
  • potassium ion transmembrane transport Source: MGI
  • potassium ion transport Source: MGI
  • regulation of long-term neuronal synaptic plasticity Source: MGI
  • regulation of membrane potential Source: MGI
  • regulation of resting membrane potential Source: MGI
  • visual perception Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296067. Potassium transport channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 10
Alternative name(s):
Inward rectifier K(+) channel Kir4.1
Potassium channel, inwardly rectifying subfamily J member 10
Gene namesi
Name:Kcnj10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1194504. Kcnj10.

Subcellular locationi

  • Membrane By similarity; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity

  • Note: In kidney distal convoluted tubules, located in the basolateral membrane where it colocalizes with KCNJ16.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6464CytoplasmicBy similarityAdd
BLAST
Transmembranei65 – 8925Helical; Name=M1By similarityAdd
BLAST
Topological domaini90 – 11425ExtracellularBy similarityAdd
BLAST
Intramembranei115 – 12612Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei127 – 1337Pore-formingBy similarity
Topological domaini134 – 1429ExtracellularBy similarity
Transmembranei143 – 16422Helical; Name=M2By similarityAdd
BLAST
Topological domaini165 – 379215CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: MGI
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: MGI
  • presynapse Source: GOC
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379ATP-sensitive inward rectifier potassium channel 10PRO_0000154954Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei338 – 3381PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9JM63.
PaxDbiQ9JM63.
PRIDEiQ9JM63.

PTM databases

iPTMnetiQ9JM63.
PhosphoSiteiQ9JM63.
SwissPalmiQ9JM63.

Expressioni

Tissue specificityi

Widely expressed in adult brain, including in the neocortex, the stratum pyrimadale of the hippocampus and the piriform cortex. Expressed by cultured astrocytes and also by cocultured cortical neurons (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000044708.
ExpressionAtlasiQ9JM63. baseline and differential.
GenevisibleiQ9JM63. MM.

Interactioni

Subunit structurei

Heterodimer with Kir5.1/KCNJ16; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. Interacts with MAGI1, alone and possibly as a heterodimer with KCNJ16; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells (By similarity). Interacts with INADL (PubMed:9647694).By similarity1 Publication

Protein-protein interaction databases

BioGridi200895. 2 interactions.
IntActiQ9JM63. 3 interactions.
MINTiMINT-1891836.
STRINGi10090.ENSMUSP00000054356.

Structurei

3D structure databases

ProteinModelPortaliQ9JM63.
SMRiQ9JM63. Positions 27-339.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi128 – 1336Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOVERGENiHBG006178.
InParanoidiQ9JM63.
KOiK05003.
OMAiGTWFAFG.
OrthoDBiEOG091G08HC.
PhylomeDBiQ9JM63.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003269. K_chnl_inward-rec_Kir1.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF21. PTHR11767:SF21. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01322. KIR12CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9JM63-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL
60 70 80 90 100
YLKDLWTTFI DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG
110 120 130 140 150
PPANHTPCVV QVHTLTGAFL FSLESQTTIG YGFRYISEEC PLAIVLLIAQ
160 170 180 190 200
LVLTTILEIF ITGTFLAKIA RPKKRAETIR FSQHAVVASH NGKPCLMIRV
210 220 230 240 250
ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV DTASDSPFLI
260 270 280 290 300
LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY
310 320 330 340 350
LPEEILWGYE FTPAISLSAS GKYIADFSLF DQVVKVASPS GLRDSTVRYG
360 370
DPEKLKLEES LREQAEKEGS ALSVRISNV
Length:379
Mass (Da):42,432
Last modified:October 1, 2000 - v1
Checksum:i7FF08446B7F43453
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039879 mRNA. Translation: BAA92432.1.
AF322631 mRNA. Translation: AAG42845.1.
CCDSiCCDS15512.1.
RefSeqiNP_001034573.1. NM_001039484.1.
XP_006496740.2. XM_006496677.2.
UniGeneiMm.254563.

Genome annotation databases

EnsembliENSMUST00000056136; ENSMUSP00000054356; ENSMUSG00000044708.
GeneIDi16513.
KEGGimmu:16513.
UCSCiuc007dqj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB039879 mRNA. Translation: BAA92432.1.
AF322631 mRNA. Translation: AAG42845.1.
CCDSiCCDS15512.1.
RefSeqiNP_001034573.1. NM_001039484.1.
XP_006496740.2. XM_006496677.2.
UniGeneiMm.254563.

3D structure databases

ProteinModelPortaliQ9JM63.
SMRiQ9JM63. Positions 27-339.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200895. 2 interactions.
IntActiQ9JM63. 3 interactions.
MINTiMINT-1891836.
STRINGi10090.ENSMUSP00000054356.

PTM databases

iPTMnetiQ9JM63.
PhosphoSiteiQ9JM63.
SwissPalmiQ9JM63.

Proteomic databases

MaxQBiQ9JM63.
PaxDbiQ9JM63.
PRIDEiQ9JM63.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056136; ENSMUSP00000054356; ENSMUSG00000044708.
GeneIDi16513.
KEGGimmu:16513.
UCSCiuc007dqj.1. mouse.

Organism-specific databases

CTDi3766.
MGIiMGI:1194504. Kcnj10.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOVERGENiHBG006178.
InParanoidiQ9JM63.
KOiK05003.
OMAiGTWFAFG.
OrthoDBiEOG091G08HC.
PhylomeDBiQ9JM63.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296067. Potassium transport channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

ChiTaRSiKcnj10. mouse.
PROiQ9JM63.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000044708.
ExpressionAtlasiQ9JM63. baseline and differential.
GenevisibleiQ9JM63. MM.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003269. K_chnl_inward-rec_Kir1.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF21. PTHR11767:SF21. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01322. KIR12CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCJ10_MOUSE
AccessioniPrimary (citable) accession number: Q9JM63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.