Q9JM63 (IRK10_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-sensitive inward rectifier potassium channel 10

Alternative name(s):
Inward rectifier K(+) channel Kir4.1
Potassium channel, inwardly rectifying subfamily J member 10

Gene names

Name:

Kcnj10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium By similarity.
Subunit structure	Seems to form heterodimer with Kir5.1/KCNJ16 By similarity. Interacts with INADL. Ref.3
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding Potassium
Molecular function	Ionic channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	adult walking behavior Inferred from mutant phenotype. Source: MGI central nervous system myelination Inferred from mutant phenotype. Source: MGI glutamate uptake involved in synaptic transmission Inferred from mutant phenotype. Source: MGI potassium ion homeostasis Inferred from mutant phenotype. Source: MGI regulation of long-term neuronal synaptic plasticity Inferred from mutant phenotype. Source: MGI regulation of resting membrane potential Inferred from mutant phenotype. Source: MGI visual perception Inferred from mutant phenotype. Source: MGI
Cellular component	basolateral plasma membrane Inferred from direct assay. Source: MGI integral to plasma membrane Inferred by curator Ref.3. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW inward rectifier potassium channel activity Inferred from mutant phenotype. Source: MGI protein binding Inferred from physical interaction Ref.3. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	ATP-sensitive inward rectifier potassium channel 10		PRO_0000154954
Regions
	Topological domain	1 – 64	64	Cytoplasmic By similarity
	Transmembrane	65 – 89	25	Helical; Name=M1; By similarity
	Topological domain	90 – 114	25	Extracellular By similarity
	Intramembrane	115 – 126	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	127 – 133	7	Pore-forming; By similarity
	Topological domain	134 – 142	9	Extracellular By similarity
	Transmembrane	143 – 164	22	Helical; Name=M2; By similarity
	Topological domain	165 – 379	215	Cytoplasmic By similarity
	Nucleotide binding	210 – 217	8	ATP Potential
	Motif	128 – 133	6	Selectivity filter By similarity
Sites
	Site	158	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9JM63 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 7FF08446B7F43453	FASTA	379	42,432
	10 20 30 40 50 60 MTSVAKVYYS QTTQTESRPL VAPGIRRRRV LTKDGRSNVR MEHIADKRFL YLKDLWTTFI 70 80 90 100 110 120 DMQWRYKLLL FSATFAGTWF LFGVVWYLVA VAHGDLLELG PPANHTPCVV QVHTLTGAFL 130 140 150 160 170 180 FSLESQTTIG YGFRYISEEC PLAIVLLIAQ LVLTTILEIF ITGTFLAKIA RPKKRAETIR 190 200 210 220 230 240 FSQHAVVASH NGKPCLMIRV ANMRKSLLIG CQVTGKLLQT HQTKEGENIR LNQVNVTFQV 250 260 270 280 290 300 DTASDSPFLI LPLTFYHVVD ETSPLKDLPL RSGEGDFELV LILSGTVEST SATCQVRTSY 310 320 330 340 350 360 LPEEILWGYE FTPAISLSAS GKYIADFSLF DQVVKVASPS GLRDSTVRYG DPEKLKLEES 370 LREQAEKEGS ALSVRISNV

References

[1]	Inanobe A., Takahashi K., Tanemoto M., Fujita A., Kurachi Y. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of an inward rectifier potassium channel gene expressed in mouse cortical astrocytes." Li L., Head V., Timpe L.C. Glia 33:57-71(2001) [PubMed: 11169792] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD-1 and ICR. Tissue: Brain.
[3]	"CIPP, a novel multivalent PDZ domain protein, selectively interacts with Kir4.0 family members, NMDA receptor subunits, neurexins, and neuroligins." Kurschner C., Mermelstein P.G., Holden W.T., Surmeier D.J. Mol. Cell. Neurosci. 11:161-172(1998) [PubMed: 9647694] [Abstract] Cited for: INTERACTION WITH INADL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB039879 mRNA. Translation: BAA92432.1. AF322631 mRNA. Translation: AAG42845.1.
IPI	IPI00124792.
RefSeq	NP_001034573.1. NM_001039484.1.
UniGene	Mm.254563.
3D structure databases
ProteinModelPortal	Q9JM63.
SMR	Q9JM63. Positions 27-353.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9JM63. 1 interaction.
STRING	Q9JM63.
Proteomic databases
PRIDE	Q9JM63.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000056136; ENSMUSP00000054356; ENSMUSG00000044708. ENSMUST00000165893; ENSMUSP00000131200; ENSMUSG00000044708.
GeneID	16513.
KEGG	mmu:16513.
Organism-specific databases
CTD	3766.
MGI	MGI:1194504. Kcnj10.
Phylogenomic databases
GeneTree	ENSGT00560000076991.
HOVERGEN	HBG006178.
InParanoid	Q9JM63.
OMA	LGPPANH.
OrthoDB	EOG483D4R.
PhylomeDB	Q9JM63.
Gene expression databases
ArrayExpress	Q9JM63.
Bgee	Q9JM63.
Genevestigator	Q9JM63.
Family and domain databases
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR001838. K_chnl_inward-rec_Kir-like. IPR003269. K_chnl_inward-rec_Kir1.2. IPR013521. K_chnl_inward-rec_Kir_Cr2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
Gene3D	G3DSA:2.60.40.1400. IR_K+channel_cytopl. 1 hit.
KO	K05003.
PANTHER	PTHR11767. K+channel_IR. 1 hit. PTHR11767:SF21. KIR12_channel. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01322. KIR12CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	289861.
SOURCE	Search...

Entry information

Entry name

IRK10_MOUSE

Accession

Primary (citable) accession number: Q9JM63

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program