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Protein

Kv channel-interacting protein 2

Gene

Kcnip2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels. Modulates channel density, inactivation kinetics and rate of recovery from inactivation in a calcium-dependent and isoform-specific manner (PubMed:16820361). In vitro, modulates KCND2/Kv4.2 and KCND3/Kv4.3 currents. Involved in KCND2 and KCND3 trafficking to the cell surface. Essential for the expression of I(To) currents in the heart (By similarity). Required for normal protein levels of KCND2 in the heart ventricle (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi189 – 200121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi237 – 248122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: RGD
  • potassium channel activity Source: UniProtKB-KW
  • potassium channel regulator activity Source: UniProtKB
  • voltage-gated ion channel activity Source: UniProtKB-KW

GO - Biological processi

  • clustering of voltage-gated potassium channels Source: RGD
  • protein heterooligomerization Source: RGD
  • regulation of potassium ion transmembrane transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Names & Taxonomyi

Protein namesi
Recommended name:
Kv channel-interacting protein 2
Short name:
KChIP2
Alternative name(s):
A-type potassium channel modulatory protein 2
Potassium channel-interacting protein 2
Gene namesi
Name:Kcnip2
Synonyms:Kchip2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi70887. Kcnip2.

Subcellular locationi

GO - Cellular componenti

  • dendrite Source: RGD
  • plasma membrane Source: UniProtKB
  • voltage-gated potassium channel complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi45 – 462CC → AA or SS: Abolishes plasma membrane localization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270Kv channel-interacting protein 2PRO_0000073824Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei9 – 91PhosphoserineCombined sources
Lipidationi45 – 451S-palmitoyl cysteine1 Publication
Lipidationi46 – 461S-palmitoyl cysteine1 Publication

Post-translational modificationi

Palmitoylated. Palmitoylation enhances association with the plasma membrane.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9JM59.
PRIDEiQ9JM59.

PTM databases

iPTMnetiQ9JM59.
SwissPalmiQ9JM59.

Expressioni

Tissue specificityi

Expressed in heart, brain and lung. In brain, abundantly expressed in striatum, hippocampus and olfactory bulb, moderately expressed in cerebral cortex and lowly expressed in thalamus and hypothalamus. Isoform 1 is predominant in cerebral cortex, striatum and hippocampus. Isoform 1, isoform 2 and isoform 3 are equally expressed in olfactory bulb. Iisoform 3 is expressed at high levels and isoform 1 at low levels in heart (in PubMed:11263977).4 Publications

Gene expression databases

ExpressionAtlasiQ9JM59. baseline and differential.
GenevisibleiQ9JM59. RN.

Interactioni

Subunit structurei

Component of heteromultimeric potassium channels. Identified in potassium channel complexes containing KCND1, KCND2, KCND3, KCNIP1, KCNIP2, KCNIP3, KCNIP4, DPP6 and DPP10 (By similarity). The KCND2-KCNIP2 channel complex contains four KCND2 and four KCNIP2 subunits (By similarity). Probably part of a complex consisting of KCNIP1, KCNIP2 isoform 3 and KCND2. At least isoform 2 and isoform 3 can self-associate to form homodimers and homotetramers. Isoform 3 interacts with KCNIP1 in a calcium-dependent manner (By similarity). Interacts with KCND2 (PubMed:16820361). Isoform 1 and isoform 3 interact with KCND3 isoform 1.By similarity3 Publications

Protein-protein interaction databases

BioGridi248591. 2 interactions.
STRINGi10116.ENSRNOP00000059337.

Structurei

3D structure databases

ProteinModelPortaliQ9JM59.
SMRiQ9JM59. Positions 90-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 13757EF-hand 1; degeneratePROSITE-ProRule annotationAdd
BLAST
Domaini140 – 17536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini176 – 21136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini224 – 25936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni257 – 27014Interaction with KCND2By similarityAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ9JM59.
OMAiYELEPTR.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ9JM59.
TreeFamiTF318560.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029532. KCNIP2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF65. PTHR23055:SF65. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9JM59-1) [UniParc]FASTAAdd to basket

Also known as: B, KCHIP2a, KChIP2L, rKCHIP2b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGQGRKESL SESRDLDGSY DQLTGHPPGP SKKALKQRFL KLLPCCGPQA
60 70 80 90 100
LPSVSETLAA PASLRPHRPR PLDPDSVEDE FELSTVCHRP EGLEQLQEQT
110 120 130 140 150
KFTRRELQVL YRGFKNECPS GIVNEENFKQ IYSQFFPQGD SSNYATFLFN
160 170 180 190 200
AFDTNHDGSV SFEDFVAGLS VILRGTIDDR LSWAFNLYDL NKDGCITKEE
210 220 230 240 250
MLDIMKSIYD MMGKYTYPAL REEAPREHVE SFFQKMDRNK DGVVTIEEFI
260 270
ESCQQDENIM RSMQLFDNVI
Length:270
Mass (Da):30,933
Last modified:August 16, 2005 - v2
Checksum:iC797DEC90FDC3B92
GO
Isoform 2 (identifier: Q9JM59-2) [UniParc]FASTAAdd to basket

Also known as: KCHIP2b

The sequence of this isoform differs from the canonical sequence as follows:
     57-75: TLAAPASLRPHRPRPLDPD → N

Show »
Length:252
Mass (Da):28,985
Checksum:i19F48D83B765295B
GO
Isoform 3 (identifier: Q9JM59-3) [UniParc]FASTAAdd to basket

Also known as: rKCHIP2a, KCHIP2L

The sequence of this isoform differs from the canonical sequence as follows:
     25-74: Missing.

Show »
Length:220
Mass (Da):25,618
Checksum:i519D144AB55A9BBC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121E → D in BAA92743 (PubMed:11263977).Curated
Sequence conflicti12 – 121E → D in BAA92744 (PubMed:11263977).Curated
Sequence conflicti31 – 311S → T in BAA92744 (PubMed:11263977).Curated
Sequence conflicti31 – 311S → T in AAH85905 (PubMed:15489334).Curated
Sequence conflicti50 – 501A → V in BAA92744 (PubMed:11263977).Curated
Sequence conflicti50 – 501A → V in AAH85905 (PubMed:15489334).Curated
Sequence conflicti105 – 1051R → K in BAA92743 (PubMed:11263977).Curated
Sequence conflicti105 – 1051R → K in BAA92744 (PubMed:11263977).Curated
Sequence conflicti143 – 1431N → T in BAA92743 (PubMed:11263977).Curated
Sequence conflicti143 – 1431N → T in BAA92744 (PubMed:11263977).Curated
Sequence conflicti177 – 1771I → V in BAA92743 (PubMed:11263977).Curated
Sequence conflicti177 – 1771I → V in BAA92744 (PubMed:11263977).Curated
Sequence conflicti182 – 1821S → N in BAA92743 (PubMed:11263977).Curated
Sequence conflicti182 – 1821S → N in BAA92744 (PubMed:11263977).Curated
Sequence conflicti255 – 2551Q → K in BAA92743 (PubMed:11263977).Curated
Sequence conflicti255 – 2551Q → K in BAA92744 (PubMed:11263977).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei25 – 7450Missing in isoform 3. 3 PublicationsVSP_015064Add
BLAST
Alternative sequencei57 – 7519TLAAP…PLDPD → N in isoform 2. 1 PublicationVSP_015065Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040031 mRNA. Translation: BAA92743.1.
AB040032 mRNA. Translation: BAA92744.1.
AF269283 mRNA. Translation: AAF81755.1.
AF269284 mRNA. Translation: AAF81756.1.
AF269285 mRNA. Translation: AAF81757.1.
BC085905 mRNA. Translation: AAH85905.1.
PIRiJC7631.
RefSeqiNP_001029133.1. NM_001033961.1. [Q9JM59-3]
NP_064479.2. NM_020094.2. [Q9JM59-1]
NP_064480.2. NM_020095.2. [Q9JM59-2]
XP_008758701.1. XM_008760479.1. [Q9JM59-1]
XP_008758702.1. XM_008760480.1. [Q9JM59-2]
XP_008758704.1. XM_008760482.1. [Q9JM59-3]
UniGeneiRn.98384.

Genome annotation databases

EnsembliENSRNOT00000024709; ENSRNOP00000024709; ENSRNOG00000018018. [Q9JM59-2]
ENSRNOT00000024750; ENSRNOP00000024750; ENSRNOG00000018018. [Q9JM59-3]
ENSRNOT00000044195; ENSRNOP00000040277; ENSRNOG00000050450. [Q9JM59-1]
ENSRNOT00000055915; ENSRNOP00000052766; ENSRNOG00000050450. [Q9JM59-2]
ENSRNOT00000086164; ENSRNOP00000073300; ENSRNOG00000050450. [Q9JM59-3]
GeneIDi100911951.
56817.
KEGGirno:100911951.
rno:56817.
UCSCiRGD:70887. rat. [Q9JM59-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB040031 mRNA. Translation: BAA92743.1.
AB040032 mRNA. Translation: BAA92744.1.
AF269283 mRNA. Translation: AAF81755.1.
AF269284 mRNA. Translation: AAF81756.1.
AF269285 mRNA. Translation: AAF81757.1.
BC085905 mRNA. Translation: AAH85905.1.
PIRiJC7631.
RefSeqiNP_001029133.1. NM_001033961.1. [Q9JM59-3]
NP_064479.2. NM_020094.2. [Q9JM59-1]
NP_064480.2. NM_020095.2. [Q9JM59-2]
XP_008758701.1. XM_008760479.1. [Q9JM59-1]
XP_008758702.1. XM_008760480.1. [Q9JM59-2]
XP_008758704.1. XM_008760482.1. [Q9JM59-3]
UniGeneiRn.98384.

3D structure databases

ProteinModelPortaliQ9JM59.
SMRiQ9JM59. Positions 90-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248591. 2 interactions.
STRINGi10116.ENSRNOP00000059337.

PTM databases

iPTMnetiQ9JM59.
SwissPalmiQ9JM59.

Proteomic databases

PaxDbiQ9JM59.
PRIDEiQ9JM59.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024709; ENSRNOP00000024709; ENSRNOG00000018018. [Q9JM59-2]
ENSRNOT00000024750; ENSRNOP00000024750; ENSRNOG00000018018. [Q9JM59-3]
ENSRNOT00000044195; ENSRNOP00000040277; ENSRNOG00000050450. [Q9JM59-1]
ENSRNOT00000055915; ENSRNOP00000052766; ENSRNOG00000050450. [Q9JM59-2]
ENSRNOT00000086164; ENSRNOP00000073300; ENSRNOG00000050450. [Q9JM59-3]
GeneIDi100911951.
56817.
KEGGirno:100911951.
rno:56817.
UCSCiRGD:70887. rat. [Q9JM59-1]

Organism-specific databases

CTDi30819.
RGDi70887. Kcnip2.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiQ9JM59.
OMAiYELEPTR.
OrthoDBiEOG7GJ6F3.
PhylomeDBiQ9JM59.
TreeFamiTF318560.

Enzyme and pathway databases

ReactomeiR-RNO-5576894. Phase 1 - inactivation of fast Na+ channels.

Miscellaneous databases

PROiQ9JM59.

Gene expression databases

ExpressionAtlasiQ9JM59. baseline and differential.
GenevisibleiQ9JM59. RN.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR029532. KCNIP2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF65. PTHR23055:SF65. 1 hit.
PfamiPF13499. EF-hand_7. 1 hit.
PF13833. EF-hand_8. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of the novel splice variants of K(+) channel-interacting protein 2."
    Ohya S., Morohashi Y., Muraki K., Tomita T., Watanabe M., Iwatsubo T., Imaizumi Y.
    Biochem. Biophys. Res. Commun. 282:96-102(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), INTERACTION WITH KCND3, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Tissue: Brain.
  2. "Palmitoylation of KChIP splicing variants is required for efficient cell surface expression of Kv4.3 channels."
    Takimoto K., Yang E.-K., Conforti L.
    J. Biol. Chem. 277:26904-26911(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, PALMITOYLATION AT CYS-45 AND CYS-46, MUTAGENESIS OF 45-CYS-CYS-46.
    Strain: Sprague-Dawley.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Tissue: Heart.
  4. Cited for: FUNCTION, INTERACTION WITH KCND2 AND KCND3, TISSUE SPECIFICITY.
  5. "Functional properties of a brain-specific NH2-terminally spliced modulator of Kv4 channels."
    Boland L.M., Jiang M., Lee S.Y., Fahrenkrug S.C., Harnett M.T., O'Grady S.M.
    Am. J. Physiol. 285:C161-C170(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "C-terminal domain of Kv4.2 and associated KChIP2 interactions regulate functional expression and gating of Kv4.2."
    Han W., Nattel S., Noguchi T., Shrier A.
    J. Biol. Chem. 281:27134-27144(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH KCND2, SUBCELLULAR LOCATION.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Localization of Kv4.2 and KChIP2 in lipid rafts and modulation of outward K(+) currents by membrane cholesterol content in rat left ventricular myocytes."
    Rudakova E., Wagner M., Frank M., Volk T.
    Pflugers Arch. 467:299-309(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiKCIP2_RAT
AccessioniPrimary (citable) accession number: Q9JM59
Secondary accession number(s): Q9JI21
, Q9JI22, Q9JI23, Q9JM60
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: August 16, 2005
Last modified: June 8, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.