Reviewed,
UniProtKB/Swiss-Prot Q9JM54 (APR_MOUSE)
Last modified
February 9, 2010.
Version 69.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: Phorbol-12-myristate-13-acetate-induced protein 1 Alternative name(s): Protein Noxa | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 103 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1 By similarity. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1. Ref.1 Ref.4 Ref.5 Ref.6 Ref.7 |
| Subunit structure | Interacts with MCL1 and BAX By similarity. Ref.1 Ref.4 Ref.5 Ref.7 |
| Subcellular location | |
| Tissue specificity | Detected in thymocytes after irradiation with X-rays. Not detectable in untreated thymocytes (at protein level). Detected in embryonic neural precursor cells of the telencephalon Constitutively expressed at low levels in adult brain, testis, thymus, spleen, lung and kidney. Ref.6 |
| Induction | Up-regulated after exposure to ionizing radiation and other genotoxic agents. Up-regulation is mediated by p53. Ref.6 |
| Domain | The BH3 motif is essential for pro-apoptotic activity. |
| Sequence similarities | Belongs to the PMAIP1 family. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| MCL1 | Q07820 | 1 | EBI-709183,EBI-1003422 | From a different organism. |
| Mcl1 | P97287 | 1 | EBI-709183,EBI-707292 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 103 | 103 | Phorbol-12-myristate-13-acetate-induced protein 1 | PRO_0000333230 | |||||||
Regions | |||||||||||
| Region | 90 – 99 | 10 | Required for mitochondrial location By similarity | ||||||||
| Motif | 27 – 35 | 9 | BH3 1 | ||||||||
| Motif | 78 – 86 | 9 | BH3 2 | ||||||||
Experimental info | |||||||||||
| Mutagenesis | 27 | 1 | L → A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-78. Ref.1 | ||||||||
| Mutagenesis | 78 | 1 | L → A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-27. Ref.1 | ||||||||
Secondary structure | |||||||||||
Helix Strand Turn | |||||||||||
| Helix | 71 – 93 | 23 | |||||||||
Sequences
References
| « Hide 'large scale' references | |
| [1] | "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." Oda E., Ohki R., Murasawa H., Nemoto J., Shibue T., Yamashita T., Tokino T., Taniguchi T., Tanaka N. Science 288:1053-1058(2000) [PubMed: 10807576] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCL1, SUBCELLULAR LOCATION, FUNCTION, MUTAGENESIS OF LEU-27 AND LEU-78. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and NOD. Tissue: Brain cortex, Dendritic cell, Kidney and Thymus. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Limb. |
| [4] | "Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins." Willis S.N., Chen L., Dewson G., Wei A., Naik E., Fletcher J.I., Adams J.M., Huang D.C.S. Genes Dev. 19:1294-1305(2005) [PubMed: 15901672] [Abstract] Cited for: FUNCTION, INTERACTION WITH MCL1. |
| [5] | "Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function." Chen L., Willis S.N., Wei A., Smith B.J., Fletcher J.I., Hinds M.G., Colman P.M., Day C.L., Adams J.M., Huang D.C.S. Mol. Cell 17:393-403(2005) [PubMed: 15694340] [Abstract] Cited for: FUNCTION, INTERACTION WITH MCL1. |
| [6] | "BH3-only proapoptotic Bcl-2 family members Noxa and Puma mediate neural precursor cell death." Akhtar R.S., Geng Y., Klocke B.J., Latham C.B., Villunger A., Michalak E.M., Strasser A., Carroll S.L., Roth K.A. J. Neurosci. 26:7257-7264(2006) [PubMed: 16822983] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY. |
| [7] | "Structural insights into the degradation of Mcl-1 induced by BH3 domains." Czabotar P.E., Lee E.F., van Delft M.F., Day C.L., Smith B.J., Huang D.C.S., Fairlie W.D., Hinds M.G., Colman P.M. Proc. Natl. Acad. Sci. U.S.A. 104:6217-6222(2007) [PubMed: 17389404] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 68-93 IN COMPLEX WITH MCL1, STRUCTURE BY NMR OF 68-94 IN COMPLEX WITH MCL1, FUNCTION, MASS SPECTROMETRY, INTERACTION WITH MCL1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | AB041230 mRNA. Translation: BAA95781.1. AK043856 mRNA. Translation: BAC31682.1. AK088556 mRNA. Translation: BAC40421.1. AK143990 mRNA. Translation: BAE25650.1. AK169914 mRNA. Translation: BAE41454.1. BC050821 mRNA. Translation: AAH50821.1. | ||||||||||||||||||||||||
| IPI | IPI00124757. | ||||||||||||||||||||||||
| RefSeq | NP_067426.1. | ||||||||||||||||||||||||
| UniGene | Mm.271878 | ||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||||||||||||||
| ModBase | Search... | ||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||
| IntAct | Q9JM54. 2 interactions. | ||||||||||||||||||||||||
| STRING | Q9JM54. | ||||||||||||||||||||||||
Proteomic databases | |||||||||||||||||||||||||
| PRIDE | Q9JM54. | ||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||
| Ensembl | ENSMUST00000025399; ENSMUSP00000025399; ENSMUSG00000024521; Mus musculus. [Genome view] | ||||||||||||||||||||||||
| GeneID | 58801. | ||||||||||||||||||||||||
| KEGG | mmu:58801. | ||||||||||||||||||||||||
| UCSC | uc008fft.1. mouse. | ||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||
| CTD | 58801. | ||||||||||||||||||||||||
| MGI | MGI:1930146. Pmaip1. | ||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||
| eggNOG | maNOG21706. | ||||||||||||||||||||||||
| HOVERGEN | Q9JM54. | ||||||||||||||||||||||||
| InParanoid | Q9JM54. | ||||||||||||||||||||||||
| OMA | RKARRNA. | ||||||||||||||||||||||||
| OrthoDB | EOG93JFRD. | ||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||
| ArrayExpress | Q9JM54. | ||||||||||||||||||||||||
| Bgee | Q9JM54. | ||||||||||||||||||||||||
| Genevestigator | Q9JM54. | ||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||
| PROSITE | PS01259. BH3. False negative. [Graphical view] | ||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||
| NextBio | 314374. | ||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||
Entry information
| Entry name | APR_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9JM54 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


