Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phorbol-12-myristate-13-acetate-induced protein 1

Gene

Pmaip1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1 (By similarity). Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1.By similarity5 Publications

GO - Biological processi

  • activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  • apoptotic process Source: MGI
  • cellular response to DNA damage stimulus Source: MGI
  • cellular response to glucose starvation Source: Ensembl
  • cellular response to hypoxia Source: Ensembl
  • defense response to virus Source: Ensembl
  • intrinsic apoptotic signaling pathway by p53 class mediator Source: UniProtKB
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
  • intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress Source: ParkinsonsUK-UCL
  • negative regulation of fibroblast proliferation Source: MGI
  • negative regulation of mitochondrial membrane potential Source: UniProtKB
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of DNA damage response, signal transduction by p53 class mediator Source: UniProtKB
  • positive regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: Ensembl
  • positive regulation of glucose metabolic process Source: Ensembl
  • positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
  • positive regulation of neuron apoptotic process Source: MGI
  • positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway Source: MGI
  • positive regulation of protein oligomerization Source: Ensembl
  • positive regulation of release of cytochrome c from mitochondria Source: Ensembl
  • proteasomal protein catabolic process Source: Ensembl
  • reactive oxygen species metabolic process Source: Ensembl
  • release of cytochrome c from mitochondria Source: UniProtKB
  • response to dsRNA Source: Ensembl
  • response to UV Source: MGI
  • response to X-ray Source: MGI
  • T cell homeostasis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

ReactomeiR-MMU-111448. Activation of NOXA and translocation to mitochondria.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.

Names & Taxonomyi

Protein namesi
Recommended name:
Phorbol-12-myristate-13-acetate-induced protein 1
Alternative name(s):
Protein Noxa
Gene namesi
Name:Pmaip1
Synonyms:Noxa
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1930146. Pmaip1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Ensembl
  • mitochondrion Source: MGI
  • nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27L → A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-78. 1 Publication1
Mutagenesisi78L → A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-27. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003332301 – 103Phorbol-12-myristate-13-acetate-induced protein 1Add BLAST103

Proteomic databases

PaxDbiQ9JM54.
PRIDEiQ9JM54.

PTM databases

iPTMnetiQ9JM54.
PhosphoSitePlusiQ9JM54.

Expressioni

Tissue specificityi

Detected in thymocytes after irradiation with X-rays. Not detectable in untreated thymocytes (at protein level). Detected in embryonic neural precursor cells of the telencephalon Constitutively expressed at low levels in adult brain, testis, thymus, spleen, lung and kidney.1 Publication

Inductioni

Up-regulated after exposure to ionizing radiation and other genotoxic agents. Up-regulation is mediated by p53.1 Publication

Gene expression databases

BgeeiENSMUSG00000024521.
GenevisibleiQ9JM54. MM.

Interactioni

Subunit structurei

Interacts with MCL1, BCL2A1 and BAX.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MCL1Q078202EBI-709183,EBI-1003422From a different organism.
Mcl1P972874EBI-709183,EBI-707292

Protein-protein interaction databases

BioGridi208439. 2 interactors.
DIPiDIP-45232N.
IntActiQ9JM54. 2 interactors.
MINTiMINT-7965607.
STRINGi10090.ENSMUSP00000025399.

Structurei

Secondary structure

1103
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi22 – 39Combined sources18
Helixi77 – 90Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JM6NMR-A68-93[»]
2NLAX-ray2.80B68-93[»]
2RODNMR-B17-42[»]
ProteinModelPortaliQ9JM54.
SMRiQ9JM54.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9JM54.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 99Required for mitochondrial locationBy similarity10

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi27 – 35BH3 19
Motifi78 – 86BH3 29

Domaini

The BH3 motif is essential for pro-apoptotic activity.

Sequence similaritiesi

Belongs to the PMAIP1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410J6XT. Eukaryota.
ENOG4111AWA. LUCA.
GeneTreeiENSGT00530000065105.
HOGENOMiHOG000034021.
HOVERGENiHBG095669.
InParanoidiQ9JM54.
KOiK10131.
OMAiADLKDEC.
OrthoDBiEOG091G1040.
TreeFamiTF339379.

Family and domain databases

InterProiIPR024140. Noxa.
[Graphical view]
PANTHERiPTHR14299. PTHR14299. 1 hit.
PfamiPF15150. PMAIP1. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9JM54-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGRKARRNA PVNPTRAELP PEFAAQLRKI GDKVYCTWSA PDITVVLAQM
60 70 80 90 100
PGKSQKSRMR SPSPTRVPAD LKDECAQLRR IGDKVNLRQK LLNLISKLFN

LVT
Length:103
Mass (Da):11,566
Last modified:October 1, 2000 - v1
Checksum:i9B9A5B04D5535E30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041230 mRNA. Translation: BAA95781.1.
AK043856 mRNA. Translation: BAC31682.1.
AK088556 mRNA. Translation: BAC40421.1.
AK143990 mRNA. Translation: BAE25650.1.
AK169914 mRNA. Translation: BAE41454.1.
BC050821 mRNA. Translation: AAH50821.1.
CCDSiCCDS29315.1.
RefSeqiNP_067426.1. NM_021451.2.
UniGeneiMm.271878.

Genome annotation databases

EnsembliENSMUST00000025399; ENSMUSP00000025399; ENSMUSG00000024521.
GeneIDi58801.
KEGGimmu:58801.
UCSCiuc008fft.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB041230 mRNA. Translation: BAA95781.1.
AK043856 mRNA. Translation: BAC31682.1.
AK088556 mRNA. Translation: BAC40421.1.
AK143990 mRNA. Translation: BAE25650.1.
AK169914 mRNA. Translation: BAE41454.1.
BC050821 mRNA. Translation: AAH50821.1.
CCDSiCCDS29315.1.
RefSeqiNP_067426.1. NM_021451.2.
UniGeneiMm.271878.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JM6NMR-A68-93[»]
2NLAX-ray2.80B68-93[»]
2RODNMR-B17-42[»]
ProteinModelPortaliQ9JM54.
SMRiQ9JM54.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208439. 2 interactors.
DIPiDIP-45232N.
IntActiQ9JM54. 2 interactors.
MINTiMINT-7965607.
STRINGi10090.ENSMUSP00000025399.

PTM databases

iPTMnetiQ9JM54.
PhosphoSitePlusiQ9JM54.

Proteomic databases

PaxDbiQ9JM54.
PRIDEiQ9JM54.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025399; ENSMUSP00000025399; ENSMUSG00000024521.
GeneIDi58801.
KEGGimmu:58801.
UCSCiuc008fft.1. mouse.

Organism-specific databases

CTDi5366.
MGIiMGI:1930146. Pmaip1.

Phylogenomic databases

eggNOGiENOG410J6XT. Eukaryota.
ENOG4111AWA. LUCA.
GeneTreeiENSGT00530000065105.
HOGENOMiHOG000034021.
HOVERGENiHBG095669.
InParanoidiQ9JM54.
KOiK10131.
OMAiADLKDEC.
OrthoDBiEOG091G1040.
TreeFamiTF339379.

Enzyme and pathway databases

ReactomeiR-MMU-111448. Activation of NOXA and translocation to mitochondria.
R-MMU-111453. BH3-only proteins associate with and inactivate anti-apoptotic BCL-2 members.

Miscellaneous databases

ChiTaRSiPmaip1. mouse.
EvolutionaryTraceiQ9JM54.
PROiQ9JM54.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024521.
GenevisibleiQ9JM54. MM.

Family and domain databases

InterProiIPR024140. Noxa.
[Graphical view]
PANTHERiPTHR14299. PTHR14299. 1 hit.
PfamiPF15150. PMAIP1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPR_MOUSE
AccessioniPrimary (citable) accession number: Q9JM54
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.